References of "Haubruge, Eric"
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See detailEvaluation of hoverfly Episyrphus balteatus De Geer (Diptera: Syrphidae) oviposition behaviour toward aphid-infested plants using a leaf disc system.
Almohamad, Raki; Verheggen, François ULg; Francis, Frédéric ULg et al

in Communications in Agricultural and Applied Biological Sciences (2006), 71(2 Pt B), 403-12

Several aphidophagous beneficials such as parasitoids and predators are known to respond positively to aphid infested plants. Semiochemicals from the latter association play an important role in the ... [more ▼]

Several aphidophagous beneficials such as parasitoids and predators are known to respond positively to aphid infested plants. Semiochemicals from the latter association play an important role in the foraging of predators in a tritrophic approach. In this work, three host plants infested with green peach aphid, Myzus persicae Sulzer were used to study the effect of prey density and aphid colony location on plant toward Episyrphus balteatus female. Their reproductive behaviour and efficiency (in terms of fecundity) were observed in net cages. Three kinds of experiments were performed using a disc leaf on agar diet in small Petri dishes under different conditions: 1) leaf disc of broad bean (Vicia faba) were infested with different aphid prey densities, 2) three host plants (Vicia faba, Solanum tuberosum, Solanum nigrum) infested with 3 different aphid prey densities and 3) three different heights of aphid colony location on V. faba plant infested with constant aphid density were also tested using leaf disc system. Aphid-free leaf discs were also used as control for predator behaviour observations. Oviposition rates of hoverfly on leaf disc system vary significantly with prey aphid densities. The means of eggs per laying were 0.9, 5.3, and 31.2 for 0, 10 and 100 aphid densities respectively. E. balteatus females were also able to evaluate and adjust oviposition rates according to different aphid prey densities/host plant. The 5 and 20 cm stakes were the most attractive heights of aphid colony location for the hoverfly oviposition. The means of eggs per laying were 16.7, 18.5 and 5.8 for 5, 20 and 40cm heights respectively. Our leaf disc system was found to be a practical and efficient way to assess chemical cues from aphids according to different conditions on the hoverfly reproductive behaviour. [less ▲]

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See detailUse of artificial diet system to assess the potential bio-insecticide effect of a fungal lectin from Xerocomus chrysenteron (XCL) on Myzus persicae.
Jaber, K.; Paquereau, L.; Fournier, D. et al

in Communications in Agricultural and Applied Biological Sciences (2006), 71(2 Pt B), 497-505

Lectins are carbohydrate binding proteins which are widely distributed in nature: they have been isolated from microorganisms, fungi, plants and animals. Many of these proteins were tested for their ... [more ▼]

Lectins are carbohydrate binding proteins which are widely distributed in nature: they have been isolated from microorganisms, fungi, plants and animals. Many of these proteins were tested for their potential biocide effect on lot of pests. Indeed, lectins can cause dramatic changes in the cellular morphology and metabolism, particularly on the digestive system of insect having ingested them, by lectin binding to membrane glycosyl groups of the digestive tract cells. A fungal lectin, namely Xerocomus Chrysenteron lectin (XCL) was previously purified and was shown to be toxic to several pests including aphids. At the cell level, an increase in the endocytosis, the induction of morphological changes such as the actin cytoskeleton shape was determined. In this work, the recombinant XCL was produced and was tested for its potential aphicide effect on Myzus persicae, a polyphagous aphid found on more than 400 host plant species and transmitting more than 100 viral diseases. We developed bioassays using different artificial diets incorporating a broad range of XCL concentrations (from 10 microgx ml(-1) to lmg.ml(-1)) to assess the potential negative effects of XCL on the development and reproduction of the M. persicae aphid. Significant mortality rates, changes of developmental durations and nymph production were observed depending on the XCL concentration in the artificial diet. Concanavalin A lectin was also used in a new range of experiments to compare the effects of the two lectins on the aphid biological parameters. According to the observed dose responses toward the XCL and Con-A lectins included in the artificial diet and the action mode of this kind of proteins, the perspectives of lectin use in pest control will be discussed. [less ▲]

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See detailPerception of aphid infested tomato plant volatiles by the predator Episyrphus balteatus
Verheggen, François ULg; Arnaud, Ludovic; Capella, Quentin et al

Poster (2006)

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See detailCharacterisation of bio-insecticide proteins in industrial residues from pea seeds (Pisum sativum L.)
Cuertero Diaz, Gaetan; Colinet, Frederic; Portetelle, Daniel ULg et al

in Abstract book (2006)

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See detailEtude de la diversité des pucerons et des auxiliaires adiphages relative à la présence d'orties en bordures de champ
Alhmedi, A.; Francis, Frédéric ULg; Bodson, Bernard ULg et al

in Notes Fauniques de Gembloux (2006), 59(2), 121-124

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See detailDéfense végétale, biotechnologies et développement de bio-insecticides
Francis, Frédéric ULg; Blecker, Christophe ULg; Deroanne, Claude et al

in Abstract book (2006)

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See detailProteomic approach to investigate aphid - plant interactions
Francis, Frédéric ULg; Harmel, Nicolas; De Pauw, Edwin ULg et al

in Abstract book (2006)

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See detailDIGE application to investigate aphid adaptation to resistant host plant
Francis, Frédéric ULg; Goggin, Fiona; Guillonneau, François et al

in Abstract book (2006)

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See detailSynthese enzymatique d’esters de sucre au départ d’huiles végétales
Detry, J.; Destain, Jacqueline ULg; Lognay, Georges ULg et al

Poster (2005, October 14)

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See detailSugar esters lipase mediated synthesis from vegetable oils
Destain, Jacqueline ULg; Detry, Jean; Lognay, Georges ULg et al

Scientific conference (2005, September 19)

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See detailSugar esters lipase mediated synthesis from vegetable oils
Destain, Jacqueline ULg; Detry, J.; Lognay, Georges ULg et al

Poster (2005, September)

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See detailGlutathione S-transferases in the adaptation to plant secondary metabolites in the Myzus persicae aphid.
Francis, Frédéric ULg; Vanhaelen, Nicolas; Haubruge, Eric ULg

in Archives of Insect Biochemistry and Physiology (2005), 58(3), 166-74

Glutathione S-transferases (GST) in insects play an important role in the detoxification of many substances including allelochemicals from plants. Induction of GST activity in Myzus persicae in response ... [more ▼]

Glutathione S-transferases (GST) in insects play an important role in the detoxification of many substances including allelochemicals from plants. Induction of GST activity in Myzus persicae in response to secondary metabolites from Brassica plants was determined using different host plant species and confirmed using artificial diet with pure allelochemicals added. The 2,4-dinitro-1-iodobenzene (DNIB) was found to be a useful substrate for identifying particular GSTs in insects. GSTs from M. persicae were purified using different affinity chromatography columns and related kinetic parameters were calculated. GST isoenzymes were characterised using electrophoretic methods. Although SDS-PAGE results indicated similarity among the purified enzymes from each affinity column, biochemical studies indicated significant differences in kinetic parameters. Finally, the GST pattern of M. persicae was discussed in terms of insect adaptation to the presence of plant secondary substances such as the glucosinolates and the isothiocyanates, from Brassicaceae host plants. [less ▲]

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See detailAphid adaptation to plant defence system
Francis, Frédéric ULg; Harmel, Nicolas ULg; Gerkens, Pascal et al

in Abstract book (2005)

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