References of "Hanikenne, Marc"
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See detailChlamydomonas reinhardtii as a eukaryotic photosynthetic model for studies of heavy metal homeostasis and tolerance
Hanikenne, Marc ULg

in New Phytologist (2003), 159(2), 331-340

The green alga Chlamydomonas reinhardtii is a useful model of a photosynthetic cell. This unicellular eukaryote has been intensively used for studies of a number of physiological processes such as ... [more ▼]

The green alga Chlamydomonas reinhardtii is a useful model of a photosynthetic cell. This unicellular eukaryote has been intensively used for studies of a number of physiological processes such as photosynthesis, respiration, nitrogen assimilation, flagella motility and basal body function. Its easy-to-manipulate and short life cycle make this organism a powerful tool for genetic analysis. Over the past 15 yr, a dramatically increased number of molecular technologies (including nuclear and organellar transformation systems, cosmid, yeast artificial chromosome (YAC) and bacterial artificial chromosome (BAC) libraries, reporter genes, RNA interference, DNA microarrays, etc.) have been applied to Chlamydomonas . Moreover, as parts of the Chlamydomonas genome project, molecular mapping, as well as whole genome and extended expressed sequence tag (EST) sequencing programs, are currently underway. These developments have allowed Chlamydomonas to become an extremely valuable model for molecular approaches to heavy metal homeostasis and tolerance in photosynthetic organisms. [less ▲]

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See detailHeavy metal hypersensitive mutants in Chlamydomonas reinhardtii.
Hanikenne, Marc ULg

Scientific conference (2002, December 16)

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See detailPleiotropic Mutants Hypersensitive to Heavy Metals and to Oxidative Stress in Chlamydomonas Reinhardtii
Hanikenne, Marc ULg; Matagne, René F.; Loppes, Roland ULg

in FEMS Microbiology Letters (2001), 196(2),

Insertional mutagenesis was used in Chlamydomonas reinhardtii to isolate original mutants hypersensitive to multiple drugs and physical agents. Out of 5200 transformants analyzed, 13 mutants belonging to ... [more ▼]

Insertional mutagenesis was used in Chlamydomonas reinhardtii to isolate original mutants hypersensitive to multiple drugs and physical agents. Out of 5200 transformants analyzed, 13 mutants belonging to seven phenotypic classes were isolated. Five were exclusively sensitive to cadmium and represented two loci. The other mutants were pleiotropic and presented a cross sensitivity to several (2--6) of the following agents: cadmium, copper, lead, paraquat, hydrogen peroxide, UVC and light. In all mutants analyzed, the hypersensitive phenotype was most probably due to a single mutational event. The sensitivity of several pleiotropic mutants to a broad range of physical and chemical agents suggests that the disrupted genes are involved in multiple stress responses. [less ▲]

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