References of "Hanikenne, Marc"
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See detailMetal accumulation in tobacco expressing Arabidopsis halleri metal hyperaccumulation gene depends on external supply.
Barabasz, Anna; Krämer, Ute; Hanikenne, Marc ULg et al

in Journal of Experimental Botany (2010), 61(11), 3057-67

Engineering enhanced transport of zinc to the aerial parts of plants is a major goal in bio-fortification. In Arabidopsis halleri, high constitutive expression of the AhHMA4 gene encoding a metal pump of ... [more ▼]

Engineering enhanced transport of zinc to the aerial parts of plants is a major goal in bio-fortification. In Arabidopsis halleri, high constitutive expression of the AhHMA4 gene encoding a metal pump of the P(1B)-ATPase family is necessary for both Zn hyperaccumulation and the full extent of Zn and Cd hypertolerance that are characteristic of this species. In this study, an AhHMA4 cDNA was introduced into N. tabacum var. Xanthi for expression under the control of its endogenous A. halleri promoter known to confer high and cell-type specific expression levels in both A. halleri and the non-hyperaccumulator A. thaliana. The transgene was expressed at similar levels in both roots and shoots upon long-term exposure to low Zn, control, and increased Zn concentrations. A down-regulation of AhHMA4 transcript levels was detected with 10 muM Zn resupply to tobacco plants cultivated in low Zn concentrations. In general, a transcriptional regulation of AhHMA4 in tobacco contrasted with the constitutively high expression previously observed in A. halleri. Differences in root/shoot partitioning of Zn and Cd between transgenic lines and the wild type were strongly dependent on metal concentrations in the hydroponic medium. Under low Zn conditions, an increased Zn accumulation in the upper leaves in the AhHMA4-expressing lines was detected. Moreover, transgenic plants exposed to cadmium accumulated less metal than the wild type. Both modifications of zinc and cadmium accumulation are noteworthy outcomes from the biofortification perspective and healthy food production. Expression of AhHMA4 may be useful in crops grown on soils poor in Zn. [less ▲]

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See detailMetal hyperaccumulation in Arabidopsis halleri: How is Zinc accumulating in the leaves?
Hanikenne, Marc ULg

Scientific conference (2009, October 02)

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See detailEvolution of metal hyperaccumulation in Arabidopsis halleri
Hanikenne, Marc ULg; Kroymann, Juergen; Talke, Ina N. et al

Conference (2009, March 04)

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See detailOrigin and evolution of SR proteins in Eukaryotes
Califice, Sophie ULg; Baurain, Denis ULg; Hanikenne, Marc ULg et al

Poster (2009, February 05)

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See detailTransition Metal Nutrition: A Balance Between Deficiency and Toxicity
Hanikenne, Marc ULg; Merchant, Sabeeha; Hamel, Patrice

in Stern, David; Harris, Elizabeth H. (Eds.) The Chlamydomonas Sourcebook, volume 2: Organellar and Metabolic Processes (2009)

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See detailMetal hyperaccumulation in Arabidopsis halleri: How is Zinc accumulating in the leaves?
Hanikenne, Marc ULg

Scientific conference (2008, October 21)

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See detailMetal hyperaccumulation in Arabidopsis halleri: How is Zinc accumulating in the leaves?
Hanikenne, Marc ULg

Scientific conference (2008, June 23)

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See detailEvolution of metal hyperaccumulation required cis-regulatory changes and triplication of HMA4
Hanikenne, Marc ULg; Talke, Ina N.; Haydon, Michael J. et al

in Nature (2008), 453

Little is known about the types of mutations underlying the evolution of species-specific traits. The metal hyperaccumulator Arabidopsis halleri has the rare ability to colonize heavy-metal-polluted soils ... [more ▼]

Little is known about the types of mutations underlying the evolution of species-specific traits. The metal hyperaccumulator Arabidopsis halleri has the rare ability to colonize heavy-metal-polluted soils, and, as an extremophile sister species of Arabidopsis thaliana, it is a powerful model for research on adaptation. A. halleri naturally accumulates and tolerates leaf concentrations as high as 2.2% zinc and 0.28% cadmium in dry biomass. On the basis of transcriptomics studies, metal hyperaccumulation in A. halleri has been associated with more than 30 candidate genes that are expressed at higher levels in A. halleri than in A. thaliana. Some of these genes have been genetically mapped to broad chromosomal segments of between 4 and 24 cM co-segregating with Zn and Cd hypertolerance. However, the in planta loss-of-function approaches required to demonstrate the contribution of a given candidate gene to metal hyperaccumulation or hypertolerance have not been pursued to date. Using RNA interference to downregulate HMA4 (HEAVY METAL ATPASE 4) expression, we show here that Zn hyperaccumulation and full hypertolerance to Cd and Zn in A. halleri depend on the metal pump HMA4. Contrary to a postulated global trans regulatory factor governing high expression of numerous metal hyperaccumulation genes, we demonstrate that enhanced expression of HMA4 in A. halleri is attributable to a combination of modified cis-regulatory sequences and copy number expansion, in comparison to A. thaliana. Transfer of an A. halleri HMA4 gene to A. thaliana recapitulates Zn partitioning into xylem vessels and the constitutive transcriptional upregulation of Zn deficiency response genes characteristic of Zn hyperaccumulators. Our results demonstrate the importance of cis-regulatory mutations and gene copy number expansion in the evolution of a complex naturally selected extreme trait. The elucidation of a natural strategy for metal hyperaccumulation enables the rational design of technologies for the clean-up of metal-contaminated soils and for bio-fortification. [less ▲]

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See detailArabidopsis halleri, a model system to understand zinc homeostasis in plants
Hanikenne, Marc ULg; Talke, Ina N.; Hamilton, Christopher ULg et al

Poster (2007, November 16)

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See detailAnalysis of the dynamic localization of atRSZp22 splicing factor by Flip-shuttling assay
Rausin, Glwadys ULg; Tillemans, Vinciane ULg; Wernimont, Pierre et al

Poster (2007, October 11)

Detailed reference viewed: 8 (1 ULg)
See detailKey roles for metal transport proteins in naturally selected Zn/Cd hyperaccumulation and tolerance in A. halleri
Talke, Ina N.; Hanikenne, Marc ULg; Ertych, N. et al

Poster (2007, June 26)

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See detailTransition metal transport
Krämer, Ute; Talke, Ina N.; Hanikenne, Marc ULg

in FEBS Letters (2007), 581(12 Sp. Iss. SI), 2263-2272

Transition metal transporters are of central importance in the plant metal homeostasis network which maintains internal metal concentrations within physiological limits. An overview is given of the ... [more ▼]

Transition metal transporters are of central importance in the plant metal homeostasis network which maintains internal metal concentrations within physiological limits. An overview is given of the functions of known transition metal transporters in the context of the unique chemical properties of their substrates. The modifications of the metal homeostasis network associated with the adaptation to an extreme metalliferous environment are illustrated in two Brassicaceae metal hyperaccumulator model plants based on cross-species transcriptomics studies. In a comparison between higher plants and unicellular algae, hypotheses are generated for evolutionary changes in metal transporter complements associated with the transition to multicellularity. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. [less ▲]

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