Three cases of infection due to Scedosporium apiopermum

Poster (1994, October)

We report three cases of infection dur to Scedosporium apiospermum in Amiens university hospital. Two cases concerned two immunosuppressed patient under corticoid tretament, but the third case concerned a ... [more ▼]

We report three cases of infection dur to Scedosporium apiospermum in Amiens university hospital. Two cases concerned two immunosuppressed patient under corticoid tretament, but the third case concerned a young healthy woman with chronic sinusitis. [less ▲]

Drug sensitivity and clinical aspects of Plasmodium falciparum malaria in african children

Conference (1994, October)

Faisabilité de la PCR dans la détection du développement de Toxoplasma gondii en culture cellulaire MRC5.

Poster (1994, June)

OBJECTIF Etudier la faisabilité de la technique PCR pour la détection du développement de Toxoplasma gondii sur milieu cellulaire à partir des culots et des surnageants de cultures sur fibroblastes ... [more ▼]

OBJECTIF Etudier la faisabilité de la technique PCR pour la détection du développement de Toxoplasma gondii sur milieu cellulaire à partir des culots et des surnageants de cultures sur fibroblastes humains MRCS. METHODES Les surnageants de culture et les cellules correspondant à différents inoculums sont prélevés après un délai variant de 24 à 120h. La technique PCR utilisée comporte une extraction à l'iodure de sodium, l'amplification d'une séquence anonyme TGRI E spécifique de T.gondii (191 paires de bases) et une révélation après électrophorèse sur gel d'agarose-bromure d'éthidium en· UV. La positivité des cultures est controlée parallèlement par immunofluorescence à l'aide d'un anticorps monoclonal antiP30 (Sanofi Pasteur) réalisée sur les culots cellulaires des mêmes cultures. RESULTATS La positivité et la cohérence des résultats erl PCR (positivité de tous les inoculums > plus faible inoculum donnant un résultat positif), aussi bien à partir des culots cellulaires que des surnageants, démontrent la faisabilité de la méthode avec en particulier l'absence d'inhibiteur de la Taq polymérase dans le milieu de culture. CONCLUSION Le développement des toxoplasmes sur milieu cellulaire peut être détecté par la technique PCR. La version sur surnageant de culture à l'intérêt de laisser intact les cellules et de témoigner du développement réel des toxoplasmes. [less ▲]

Evaluation de la chimiorésistance de Plasmodium falciparum. Revue des tests in vitro: méthodologie, intérêts et limites

in Publications Médicales Africaines (1994), 129

The chloroquine-resistance of Plasmodium falciparum appeared first in Southeast Asia and South-America during the sixties. It reached further the african continent in 1977, through Kenya and Tanzania. In ... [more ▼]

The chloroquine-resistance of Plasmodium falciparum appeared first in Southeast Asia and South-America during the sixties. It reached further the african continent in 1977, through Kenya and Tanzania. In Africa, the front of the resistance spread from East to West, and now completely covers Black Africa with different degrees of resistance. Rapidly, "in vivo" and "in vitro" tests were developped to assess the sensitivity of P. falciparum strains against the different antimalarial drugs. This paper is a review of existing "in vitro" and "in vivo" tests with their interest and limits. [less ▲]

Histoplasmose africiaine à Histoplasma duboisii (Histoplasma capsulatum var. duboisii): quatorze cas congolais observés en 10 ans (1981-1990)

in Journal de Mycologie Médicale (1993), 3

Fourteen cases of histoplasmosis due to Histoplasma duboisii seen in Congo from 1981 through 1990, are reported: the average age of the patients was 25 years (the youngest being 1,5 y and the oldest 50y ... [more ▼]

Fourteen cases of histoplasmosis due to Histoplasma duboisii seen in Congo from 1981 through 1990, are reported: the average age of the patients was 25 years (the youngest being 1,5 y and the oldest 50y), the M/F ratio was 2,5; most of the patients (10/14) live in a rural area. there was a rather constant frequency of one to three cases per annum from 1981 through 1990. The fisrt congolese AIDS-associated systemic histoplasmosis duboisii case was observed in 1990. Lymph node involvment and mucocutaneous lesions were the most frequent clinical manifestations. In all cases, the disgnosis was established by histological examination. When performed, direct microscopy was always positive (11/11). Isolation of H. duboisii in culture was successful in 7 out of 9 attempts. Two cases of systemic infection were rapidly fatal but a good clinical response was obtained in the other cases wih amphotericin B, which due to intolerance, was replaced by ketoconazole in 2 patients. [less ▲]

Presence of human antibodies reacting with Candida albicans O-linked oligomannosides revealed by using an enzyme-linked immunosorbent assay and neoglycolipids

in Journal of Clinical Microbiology (1992), 30(2), 411-417

In order to study the presence of antibodies directed against Candida albicans O-linked oligomannosides (oligomannosides O) in patient sera, we have developed an enzyme-linked immunosorbent assay (ELISA ... [more ▼]

In order to study the presence of antibodies directed against Candida albicans O-linked oligomannosides (oligomannosides O) in patient sera, we have developed an enzyme-linked immunosorbent assay (ELISA) involving neoglycolipids constructed with these residues (NGLO). Oligomannosides O released by mild alkaline degradation of the C. albicans cell wall phosphopeptidomannan (PPM) contained one to seven mannose residues, among which the quantitatively major components, mannobiose and mannotriose, were shown by 1H nuclear magnetic resonance to contain exclusively alpha (1-2) linkages. The pool of oligomannosides was converted to neoglycolipids by coupling them to 4-hexadecylaniline in an equimolar reaction checked by thin-layer chromatography. We have tested against these neoantigens, coated on ELISA plates, 15 pairs of sera corresponding to individual seroconversions observed in 15 patients during the course of a mycological and serological survey of candidiasis. For all patients, seroconversions resulted in an increased level of antibodies against NGLO. A significant correlation was observed between the results of ELISA-NGLO, ELISA involving the original PPM molecule, and routine antibody detection tests, indirect immunofluorescence assay, and cocounterimmunoelectrophoresis. These results therefore demonstrate the synthesis of human antibodies reactive with oligomannosides O constitutive of the C. albicans mannan molecule which have been previously described as exhibiting an inhibitory effect on human lymphocytic proliferation. [less ▲]

Immunocytochimie et diagnostic des leucémies aigües: mise au point d'une technique utilisant le complexe phosphatase alcaline-anticorps anti-phosphatase alcaline.

Master of advanced studies dissertation (1990)

Le diagnostic des leucémies aigues (LA) bénéficie à l'heure actuelle de différents outils complémentaires. Même si la classification FAB (Franco-Américaine-Britannique) reste la référence, il n'en demeure ... [more ▼]

Le diagnostic des leucémies aigues (LA) bénéficie à l'heure actuelle de différents outils complémentaires. Même si la classification FAB (Franco-Américaine-Britannique) reste la référence, il n'en demeure pas moins vrai que l'introduction des anticorps (Ac) monoclonaux a ouvert des perspectives nouvelles en associant des profils immunologiques aux différents types de leucémies aiguës. Des techniques immunocytochimiques utilisant différents enzymes, phosphatases alcaline et peroxydase, associés à des systèmes d'amplification, complexes phosphatase alcaline anticorps anti-phosphatase alcaline (APAAP) ou peroxydase anticorps anti-peroxydase (PAP) ou avidine-biotine peroxydase (ABC), permettent de travailler directement sur frottis sanguins ou médullaires. Ce travail propose une mise au point de la technique à l'APAAP sur lame, afin de répondre au problème posé par les moelles pauvres en blastes dont l'étude est difficile à réaliser par immunofluorescence en cytométrie de flux. [less ▲]