References of "HAYETTE, Marie-Pierre"
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See detailEchinoccose alvéolaire : l'expérience liégeoise
Hayette, Marie-Pierre ULg

Conference (2012, June 09)

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See detailEvaluation of three immunoassays for serodiagnosis of human Mycoplasma pneumoniae infection
HUYNEN, Pascale ULg; TOUSSAINT, Françoise ULg; HAYETTE, Marie-Pierre ULg et al

in Clinical Microbiology & Infection (2012, April), 18(S3), 231

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See detailEvaluation of three immunoassays for serodiagnosis of human Mycoplasma pneumoniae infection
HUYNEN, Pascale ULg; TOUSSAINT, Françoise ULg; HAYETTE, Marie-Pierre ULg et al

Poster (2012, April)

The aim of this study was to evaluate three commercial automated immunoassays for the serological diagnosis of M. pneumoniae infection.

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See detailFilamentous fungi recovered from the water distribution system of a Belgian university hospital
Hayette, Marie-Pierre ULg; Christiaens, Geneviève ULg; Mutsers, Jacques ULg et al

in Medical Mycology (2010), 48(7), 969-974

A study was carried out over a 4-month winter period in order to assess the presence of filamentous fungi in the water distribution system of the University Hospital of Liège. A total of 197 hot and cold ... [more ▼]

A study was carried out over a 4-month winter period in order to assess the presence of filamentous fungi in the water distribution system of the University Hospital of Liège. A total of 197 hot and cold water samples were collected from the main water supply lines and from the taps at three different hospital sites. Overall, filamentous fungi were recovered from 55% and 50% of the main water distribution system and tap water samples, respectively, with a mean of 3.5 ± 1.5 colony forming units per 500 ml water. Nine different genera were identified, all belonging to the Hyphomycetes class. Aspergillus spp. were recovered from 6% of the samples of the water distribution system and A. fumigatus was the most frequently recovered species (66.6%). However, this species was not isolated from water taps. Fusarium spp. was predominant at one site, where it was found in 28% of tap water samples. No Aspergillus spp. but some Fusarium spp. isolates were identified in samples collected from high-risk units. Filters were introduced at the point-of-use in the haematology unit after completion of the study. The findings of the present study confirm the need for further documented studies to evaluate the safety of the hospital water system and to define new preventive measures. [less ▲]

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See detailAspergillus: still the same as before?
Hayette, Marie-Pierre ULg

Conference (2010, October 07)

La présentation fait le point sur les évolutions touchant les Aspergillus depuis une vingtaine d'années sur les plan taxonomique, épidémiologique, clinique et thérapeutique.

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See detailComparative studies for the serodiagnosis of Chlamydophila and Mycoplasma pneumoniae infections
Huynen, Pascale ULg; Goebel, Marie-Rose ULg; Meex, Cécile ULg et al

in ESCMID (Ed.) Abstract book of the 20th ECCMID (2010, April)

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See detailTravaux pratiques de microbiologie générale
HAYETTE, Marie-Pierre ULg; HUYNEN, Pascale ULg; MEEX, Cécile ULg

Learning material (2010)

Le syllabus reprend les méthodologies utilisées pour l'étude des bactéries, levures, virus. Il décrit les réactions sérologiques et les principes de biologie moléculaire appliqués au diagnostic ... [more ▼]

Le syllabus reprend les méthodologies utilisées pour l'étude des bactéries, levures, virus. Il décrit les réactions sérologiques et les principes de biologie moléculaire appliqués au diagnostic microbiologique. [less ▲]

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See detailSpectrométrie de masse MALDI-TOF en bactériologie clinique ou comment identifier une bactérie en une minute
Descy, Julie ULg; Meex, Cécile ULg; Melin, Pierrette ULg et al

in Revue Médicale de Liège (2010), 65(Suppl. Synthèse 2010), 29-34

The major application of MALDI-TOTOF mass spectrometry in clinical microbiology is the bacterial identification based on the analysis of all their proteins (ribosomal and membrane-associated proteins ... [more ▼]

The major application of MALDI-TOTOF mass spectrometry in clinical microbiology is the bacterial identification based on the analysis of all their proteins (ribosomal and membrane-associated proteins). This technology allows the identification of most of bacteria within a few minutes. The method is fast, accurate, reliable and cost-effective by comparison to conventional phenotypic techniques. Other applications of MALDI-TOF mass spectrometry are still under development, as the detection of bacterial toxins or resistance mechanisms to antimicrobial agents. [less ▲]

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See detailMucormycose invasive du poumon et du rachis dorsal.
De Pasqual, Aurelie ULg; Deprez, Manuel ULg; Ghaye, Benoît ULg et al

in Revue Médicale de Liège (2008), 63

Nous rapportons le cas d'un patient de 67 ans atteint d'un syndrome myélodysplasique et qui a développé une mucormycose pulmonaire avec extension tout à fait exceptionnelle vers le rachis dorsal ... [more ▼]

Nous rapportons le cas d'un patient de 67 ans atteint d'un syndrome myélodysplasique et qui a développé une mucormycose pulmonaire avec extension tout à fait exceptionnelle vers le rachis dorsal responsable d'un paraplégie aiguë. Après échec d'un traitement probabiliste anti-aspergillaire, c'est finalement l'analyse des prélèvements obtenus lors de la laminectomie décompressive qui a fourni le diagnostic mycologique. En raison d'une altération majeure de l'état général, la lobectomie prévue n'a pu être réalisée et malgré l'adaptation du traitement antifongique (Abelcet, Posaconazole), le patient est décédé. La mucormycose (ou zygomycose) pulmonaire est une infection fongique peu commune qui touche essentiellement les patients immuno-déprimés. Le champignon pathogène fait partie des zygomycètes dont la caractéristique principale est la capacité d'angio-invasion. L'invasion périneurale est une autre voie de propagation récemment mise en évidence. Les difficultés thérapeutiques associées à cette pathologie sont liées au terrain d'immunodépression, aux difficultés d'obtenir rapidement un diagnostic précis ainsi qu'à l'absence de sensibilité du Mucor aux antifongiques récemment introduits (V-Fend, Cancidas). Ceci souligne le risque inhérent à un traitement antifongique empirique par ces agents et la nécessité d'un prélèvement biopsique précoce en cas de non-réponse au traitement. [less ▲]

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See detailA Patient with Hiv Infection, Cough, Asthenia, and Fever
Mayasi, N.; Chandrikakumari, Kavitha; Mukeba, D. et al

in Clinical Infectious Diseases : An Official Publication of the Infectious Diseases Society of America (2007), 45(5), 662-3559-600

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See detailDiagnostic moléculaire de la malaria
Hayette, Marie-Pierre ULg

Scientific conference (2007, May 30)

Le cours porte sur les intérêts et limites des techniques de biologie moléculaire appliquées au diagnostic du paludisme.

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See detailLIAISON® VZV IgG and VZV IgM assays: a comparative study
HUYNEN, Pascale ULg; MELIN, Pierrette ULg; HAYETTE, Marie-Pierre ULg et al

in Clinical Microbiology & Infection (2007, April), 13(Suppl. S1), 639

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See detailEvaluation of a new commercial real time PCR for the detection of Aspergillus spp. in serum and respiratory samples
Hayette, Marie-Pierre ULg; Meex, Cécile ULg; Boreux, Raphaël ULg et al

Poster (2007, April)

Objectives. Diagnosis of invasive aspergillosis is still disappointing and often delayed because of the lack of sensitivity of diagnostic tools. DNA detection based-methods have been developed, but differ ... [more ▼]

Objectives. Diagnosis of invasive aspergillosis is still disappointing and often delayed because of the lack of sensitivity of diagnostic tools. DNA detection based-methods have been developed, but differ widely and comparisons are difficult to assess. The objective of the study is to compare a new commercial real-time PCR kit, affigene® Aspergillus tracer assay, with an in house nested PCR targeting 18S rRNA Aspergillus sp. gene. Methods. Twelve patients at risk for invasive aspergillosis were included in the study. They were classified to have possible (5 cases), probable (1 case) or proven (6 cases) invasive aspergillosis following E.O.R.T.C. criteria. Fifteen serum and respiratory paired samples were collected. The DNA extraction was performed by using the QIAmp DNA mini kit® (Qiagen, Germany). All samples were tested by both PCR assays and respiratory samples were cultured. Results. Respiratory samples. A. fumigatus, A. niger and A. flavus were isolated from 10/15 samples; both PCR methods were positive for these samples except one that was positive for affigene® and equivocal for the nested PCR. The real-time PCR assay reported cycle thresholds ranging from 25 to 38. Three of the five culture-negative samples were negative by both PCR methods; one of three was negative in affigene® assay and equivocal by nested PCR; the last sample was positive in affigene® assay and negative by nested PCR. Serum. Thirteen of fifteen blood samples were negative by both PCR methods. One sample was equivocal by nested PCR and was inhibited in affigene® assay despite a culture-positive paired respiratory sample. The last case was inhibited by the real-time PCR assay and negative by nested PCR. Nor the nested PCR, nor affigene® assay could detect any Aspergillus DNA in serum. In total, there was 93% of agreement between the two PCR assays. Conclusion. Both methods are in good agreement and can detect at least three different species of Aspergillus. However, the sensitivity of both assays does not permit the detection of Aspergillus DNA in serum. affigene® assay can easy replace the “in house” assay: it allows a fast and standardized detection of Aspergillus sp. DNA in respiratory samples without inconvenient due to the handling of PCR products. [less ▲]

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See detailDisseminated Penicillium marneffei infection contrated in China
Hayette, Marie-Pierre ULg; Mukeba Tschialala, Didier; Meex, Cécile ULg et al

Poster (2007)

Penicillium marneffei infection is a rare fungal disease that cause significant disease in immunosuppressed patients. The geographical distribution of this dimorphic fungus is restricted to Asia ... [more ▼]

Penicillium marneffei infection is a rare fungal disease that cause significant disease in immunosuppressed patients. The geographical distribution of this dimorphic fungus is restricted to Asia, Southeast and Far East, where the disease is considered as an indicator of acquired immunodeficiency syndrome (AIDS). Case report. A 42-year old Congolese woman living in Lubumbashi was admitted at the university hospital of Liège for exploration of a general status impairment. She experienced for three months spiking fever, weight loss, productive cough with bloody expectorations and progressive dyspnoea. She reported also to have non-bloody mild diarrhoea with abdominal pain. The HIV antibody status was positive with a low CD4 T lymphocytes count (28/µl). Pulmonary infiltrates were visualized on chest radiography and the computed tomography revealed the presence of a severe pneumopathy characterised by bilateral micronodular lesions. Mediastinal polyadenopathies associated with hepato- and splenomegaly were also highlighted. Bronchoscopy was performed and bronchial aspirations revealed the presence of numerous leucocytes with the presence of intracellular Gram positive organisms suggestive of yeasts. Ziehl, Giemsa and Gomori-Grocott staining were also performed. Ziehl staining was negative. The morphological aspect given by Giemsa staining excluded infection and the PCR specific for T. gondii B1 gene was negative. However, Gomori-Grocott staining revealed the presence of intracellular oval, elongated, sausage-shaped cells with a single transverse septum (3 to 5 µm). Penicillium marneffei was isolated from blood culture and respiratory samples. Intraveinous amphotericin B treatment was administrated during 15 days followed by itraconazole oral administration (200 mg/j). The antimycotic treatment improved the patient condition and despite other clinical troubles she was prematurely discharged because of financial problems. Conclusion. Opportunistic agents involved in HIV-infected patients differ in Africa and Asia and it is important to be able to make a rapid diagnosis with the aid of an experienced laboratory. [less ▲]

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See detailPericardite aspergillaire avec tamponnade cardiaque et syndrome hemophagocytaire: un cas non classique d'immunodeficience
Delcroix, Geoffrey ULg; Vanstraelen, G.; Hustinx, Roland ULg et al

in Revue Médicale de Liège (2006), 61(10), 713-8

We report the case of a 31-year-old woman who died in a context of haemophagocytic syndrome with multiple opportunist infections:viral, mycobacterial and fungal. To our knowledge, this is the tenth case ... [more ▼]

We report the case of a 31-year-old woman who died in a context of haemophagocytic syndrome with multiple opportunist infections:viral, mycobacterial and fungal. To our knowledge, this is the tenth case of invasive aspergillosis manifested by an aspergillus pericarditis with cardiac tamponade. Search for HIV infection, neoplasia, haematological malignancies was negative. In addition, the patient carried on a nonviral, non-ethylic cirrhosis, the etiology of which remained unknow. We will particularly develop the aspergillus pericarditis with cardiac tamponade and the haemophagocytic syndrome. [less ▲]

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See detailMolecular epidemiology of extended-spectrum beta-lactamase-producing Enterobacteriaceae colonizing the digestive tract of patients admitted to intensive care units in a Belgian university hospital
CHRISTIAENS, Geneviève ULg; Damas, Pierre ULg; Docquier, J. D. et al

in American Society of Microbiology (Ed.) Program and Abstracts of the 46th Intersciences Conference on Antimicrobial Agents and Chemotherapy (2006, September)

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See detailEvaluation of the Strepto B ID agar for the detection of group B streptococci from vaginal and recto-vaginal specimens
MELIN, Pierrette ULg; Bonafe, S.; HAYETTE, Marie-Pierre ULg et al

in ASM (Ed.) Program and Abstracts of the 106th General Meeting of the American Society for Microbiology (2006, May)

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