References of "Guyot, Hugues"
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See detailEvaluation of a Bovine Concentrated Lactoserum for Preventing Neonatal Diarrhoea in Belgian Blue Calves
Vandeputte, Sébastien ULg; Rollin, Frédéric ULg; Guyot, Hugues ULg et al

in Open Veterinary Science Journal (The) (2010), 4

The purpose of this study was to evaluate, under field conditions, the efficacy of an european registered bovine concentrated lactoserum (Locatim) in 3 farms with neonatal diarrhoea in calves. A total of ... [more ▼]

The purpose of this study was to evaluate, under field conditions, the efficacy of an european registered bovine concentrated lactoserum (Locatim) in 3 farms with neonatal diarrhoea in calves. A total of 117 healthy Belgian Blue (BB) calves were allocated in 2 groups. Two thirds of the calves received Locatim orally immediately after birth and maternal colostrum one hour later (treated group), while control calves only received maternal colostrum. Every day during 14 days, mental status, faeces consistency, suckling reflex and hydration status of each calf were monitored. Individual blood samples were assessed for passive transfer and specific Escherichia coli antibodies against strains F5, CS31A, F17 and F41. Faecal samples from diarrheic and non diarrheic calves were analysed for rotavirus, bovine coronavirus, Cryptosporidium parvum and Escherichia coli F5. Locatim had no significant effect on the onset, duration and incidence of diarrhoea. The mean serum IgG concentration of 23.1 ± 7.8 mg/ml indicates a good IgG transfer. Only the CS31A strain titer was significantly higher in the treated group. The major identified causative agent of diarrhoea was C. parvum. In conclusion, Locatim only has a slight effect when IgG transfer is optimal, but could be justified when specific antibodies lacking in colostrum are needed [less ▲]

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See detailGenomic expression evaluation: introduction and perspectives
Ramery, Eve ULg; Bureau, Fabrice ULg; Guyot, Hugues ULg et al

Conference (2010, July)

In a way, disease could be considered as the result of gene expression deregulation. Knowing what genes are expressed in a particular cell or tissue and how they are regulated allow to better understand ... [more ▼]

In a way, disease could be considered as the result of gene expression deregulation. Knowing what genes are expressed in a particular cell or tissue and how they are regulated allow to better understand disease mechanisms. Transcriptomic is the study of the expression of the entire genome. This technical challenge has been rendered possible as different species’ genomes began to be sequenced. Several techniques have rendered efficient this process of measuring simultaneously the expression level of a large number of genes. To date, micro-array technology is probably the best known. However, with the development of more affordable techniques, sequencing/ resequencing could compete with microarrays in the future. All together, these techniques open new ways for early diagnosis and prognosis for the practioner ‘in the field’. [less ▲]

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See detailLa maladie de Mortellaro: un fléau insidieux dans les élevages bovins
Guyot, Hugues ULg

Article for general public (2009)

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See detailInfection expérimentale de veaux par le virus de la fièvre catarrhale ovine de sérotype 8
Dal Pozzo, Fabiana ULg; De Clercq, K.; Guyot, Hugues ULg et al

in Epidémiologie et Santé Animale (2009), 55

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See detailExperimental reproduction of bluetongue virus serotype 8 clinical disease in calves.
Dal Pozzo, Fabiana ULg; De Clercq, Kris; Guyot, Hugues ULg et al

in Veterinary Microbiology (2009), 136(3-4), 352-8

Cattle are commonly subclinically infected following natural or experimental infection with bluetongue virus (BTV). The introduction of BTV serotype 8 (BTV-8) in Europe has been characterized by the ... [more ▼]

Cattle are commonly subclinically infected following natural or experimental infection with bluetongue virus (BTV). The introduction of BTV serotype 8 (BTV-8) in Europe has been characterized by the manifestation of clinical signs in infected cattle. In order to study the pathogenesis of BTV-8 in this host, an animal model able to reproduce the clinical manifestations of the disease is required. In this work, two calves were subcutaneously and intravenously injected with a low passage cell-adapted strain of BTV-8. Both calves showed typical bluetongue clinical signs, including pyrexia, ocular discharge, conjunctivitis, oral mucosal congestion, development of ulcers and necrotic lesions on the lips and tongue, submandibular oedema, coronitis and oedema of the coronet and pastern region. A score was assigned depending on the severity of the lesions and a total clinical score was calculated for each animal daily and at the end of the experiment. Both calves became viraemic 24h post-infection and seroconversion occurred between 7 and 11 days P.I. In this study we present the development of a protocol of infection in calves able to reproduce the severity of the lesions observed with BTV-8 in field conditions. [less ▲]

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See detailEpidemiology of trace elements deficiencies in Belgian beef and dairy cattle herds.
Guyot, Hugues ULg; Saegerman, Claude ULg; Lebreton, Pascal et al

in Journal of Trace Elements in Medicine and Biology (2009), 23(2), 116-23

Selenium (Se), iodine (I), zinc (Zn) and copper (Cu) deficiencies in cattle have been reported in Europe. These deficiencies are often associated with diseases. The aim of the study was to assess trace ... [more ▼]

Selenium (Se), iodine (I), zinc (Zn) and copper (Cu) deficiencies in cattle have been reported in Europe. These deficiencies are often associated with diseases. The aim of the study was to assess trace element status in Belgian cattle herds showing pathologies and to compare them to healthy cattle herds. Eighty-two beef herds with pathologies, 11 healthy beef herds, 65 dairy herds with pathologies and 20 healthy dairy herds were studied during barn period. Blood and/or milk samples were taken in healthy animals. Plasma Zn, Cu, inorganic I (PII) and activity of glutathione peroxidase in erythrocytes (GPX) were assayed. In milk, I concentration was measured. Data about pathologies and nutrition in the herds were collected. According to defined thresholds, it appeared that a large proportion of deficient herds belonged to "sick" group of herds. This conclusion was supported by the mean value of trace elements and by the fact that a majority of individual values of trace elements was below the threshold. Dairy herds had mean values of trace elements higher than beef herds. More concentrates and minerals were used in healthy herds versus "sick" herds. These feed supplements were also used more often in dairy herds, compared to beef herds. Trace elements deficiencies are present in cattle herds in Belgium and are linked to diseases. Nutrition plays a major role in the trace elements status. [less ▲]

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See detailNutritional markers course after oral supplementation with different forms of iodine in Holstein non-lactating cows
Guyot, Hugues ULg; VanParijs, Sandrine; Uyttenhoef, Aude et al

Poster (2009)

Iodine (I) deficiency is commonly reported in cattle around the world and is often associated with clinical or sub-clinical diseases. As most of clinical signs are not pathognomonic, diagnosis has to be ... [more ▼]

Iodine (I) deficiency is commonly reported in cattle around the world and is often associated with clinical or sub-clinical diseases. As most of clinical signs are not pathognomonic, diagnosis has to be confirmed by biochemical analyses such as plasmatic inorganic iodine (PII) or urinary I. Different oral mineral forms of I are available in Europe for cattle. The aim of the study was to compare the kinetic of I in blood and urine in non-lactating cows, following oral administration of different forms of I. Five groups of 6 non-lactating cows (aged 6 ± 2 years, weight 604 ± 89 kg), receiving the same ration (11 kg dry matter) and housed in the same conditions (tied-stall and straw) underwent a double-blinded trial during 2 months. Excepting in Group A (Control), all cows received a daily oral supplementation of I equal to 5 ppm, in the form of Ca(IO3)2 (Group B), KI (Group C), organic form of I 1 (Group D) and organic form of I 2 (Group E). Formulas of the organic forms of I are not public and coverable by patent. Supplementation was stopped at T45. Blood and urine samples were taken at T0, T15, T30 and T60. Thyroxine (T4) was measured at T0, T30 and T60 while PII and urinary I were measured at the 4 times of the trial. Student-t test and multiple comparisons of means (mix crossed model) were used to compare I and T4 concentrations between groups and times. All characteristics about the cows and I levels in blood or urine were not significantly different at T0 (p>0.1). There was no significant difference (p>0.1) between groups B, C, D, E at the different times of the trial. PII and urinary I in Group A were significantly lower than in other groups (p<0.01) at T15 and T30. Highest concentrations of I (PII up to 242 ± 30 µg/L and urinary I up to 2326 ± 439 µg/L) were reached at T15 for groups B, D and E. At T60, PII (19 ± 4 µg/L) and urinary I (110 ± 29 µg/L) of all groups reached the basal level. A good correlation was found between PII and urinary I (r² = 0.77). No significant differences were found about T4 (67 ± 10 nmol/L) in all groups and times (p>0.1). PII and urinary I are good markers to assess I nutritional status. No difference was found between either inorganic or organic forms of I, nor between them. Concerning the mineral forms of I, Ca(IO3)2 might be preferably used because of its higher stability in the mineral complexes. [less ▲]

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See detailSerum cardiac troponin I concentrations as a diagnostic aid of pericarditis in cattle
Guyot, Hugues ULg; Sartelet, Arnaud ULg; Leidinger, K. et al

Poster (2008, July 10)

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See detailWhole blood viscosity and viscoelasticity in healthy dairy cows
Sandersen, Charlotte ULg; Dringenberg, B.; Vandeputte, Sébastien ULg et al

Poster (2008, July 10)

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See detailVegetative endocarditis in the horse: retrospective study
Porter, Sarah ULg; Saegerman, Claude ULg; Van Galen, Gaby ULg et al

in Journal of Veterinary Internal Medicine (2008), 22(6), 1411-1416

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See detailBluetongue in captive yaks.
Mauroy, Axel ULg; Guyot, Hugues ULg; De Clercq, Kris et al

in Emerging Infectious Diseases (2008), 14(4), 675-6

In August 2006, several Northern European countries including Belgium reported their first cases of bluetongue (BT). Surprisingly, it was the first time that BT was diagnosed so far in the northern ... [more ▼]

In August 2006, several Northern European countries including Belgium reported their first cases of bluetongue (BT). Surprisingly, it was the first time that BT was diagnosed so far in the northern hemisphere (1). BT is a non contagious, arthropod borne animal disease. The causal virus belongs to the genus Orbivirus in the family Reoviridae. The genome of the bluetongue virus (BTV) consists of 10 segments of double-stranded RNA and 24 serotypes have been reported (2). Serotype 8 (BTV-8) was implied in the emergence in Belgium (3). All ruminant species are thought to be susceptible to BT (2) but lack of data remains for certain species. We report here laboratory confirmed clinical cases of BT in yaks. [less ▲]

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See detailClinical aspects of bluetongue in ruminants
Guyot, Hugues ULg; Mauroy, Axel ULg; Kirschvink, N. et al

in Bluetongue in northern Europe (2008)

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See detailDifferential diagnosis of bluetongue
Bexiga, R.; Guyot, Hugues ULg; Saegerman, Claude ULg

in Bluetongue in northern Europe (2008)

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See detailEvolution of the clinical expression of Bluetongue in Belgian cattle during year 2006 vs 2007
Guyot, Hugues ULg; Mauroy, Axel ULg; Rollin, Frédéric ULg et al

Conference (2008)

In August 2006, Belgium notified its first cases of Bluetongue (BT), serotype 8, in cattle and sheep. The disease was also observed at this time in the neighboring countries. The resurgence of BT was ... [more ▼]

In August 2006, Belgium notified its first cases of Bluetongue (BT), serotype 8, in cattle and sheep. The disease was also observed at this time in the neighboring countries. The resurgence of BT was observed in Northern Europe in 2007. The aim of the study was to compare clinical signs of BT observed in 2006 vs 2007 in Belgian cattle. The description of clinical signs was based on the observation of 38 and 39 cows in 2006 and 2007, respectively. BT cases were only included if they were confirmed by one or both laboratory diagnostic tests (competitive ELISA test and/or RT-qPCR). The inventory of clinical signs was made with a standardised clinical form for BT. This form is divided into general, cutaneous, locomotor, digestive, respiratory, neurological and reproductive clinical signs. Case data were summarised to determine changes in clinical presentation of BT between 2006 and 2007. A Fischer’s exact probability test was performed to compare (P<0.05) the frequency of clinical signs between the two years. Regarding general clinical signs, hyperthermia and tiredness were more often observed in 2007, compared to 2006. All clinical signs about skin and annexes were not significantly different between the two years. Locomotor signs such as prostration, incapacity to get up, reluctance to move, lameness and amyotrophy were more frequent in 2007. Loss of appetite, difficulties in grasping feed, salivation and drooling were the digestive signs more often observed in 2007. A purulent nasal discharge was the only respiratory sign more commonly observed in 2007. Apathy, generalised weakness and paresis or paralysis were more often encountered in 2007. The most important changes between the two years concerned reproduction. A higher incidence of abortion, premature calving and stillbirth was observed during 2007 outbreak. The frequency of most of the clinical signs of BT was higher in 2007 in Belgian cattle. Confirmed cases of BT in Belgian cattle were only 296 in 2006 compared to 4187 in 2007. These data do not represent the real situation of BT infection because the farmers do not notify all cases. Nevertheless, it seems that the 2007 outbreak was more severe regarding the number of cases and the frequency of clinical signs. The mild winter and wet 2007 summer might have favored the persistence of the vectors. [less ▲]

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