References of "Guyot, Hugues"
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See detailSuccessful reduction of cholelithiasis in a Holstein cow
Lamain, Guillaume ULg; Frisee, Vincent ULg; Ramery, Eve ULg et al

Poster (2011, September)

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See detailAccuracy of inflammation field tests in cattle practice
Guyot, Hugues ULg; Lamain, Guillaume ULg; Detilleux, Johann ULg et al

in Société Belge Francophone de Buiatrie & Vlaamse vereniging voor Buiatrie (Ed.) Proceedings of the 6th European Congress of Bovine Health Management (2011, September)

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See detailTetanus following castration in a buck
Lamain, Guillaume ULg; Guyot, Hugues ULg

Poster (2011, September)

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See detailDevelopment of a scoring system to assess lameness status in dairy cattle farms
Frisee, Vincent ULg; Guyot, Hugues ULg

in Société Belge Francophone de Buiatrie & Vlaamse vereniging voor Buiatrie (Ed.) Proceeding of the 6th European Congress of Bovine Health Management (2011, September)

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See detailOutils diagnostiques des maladies infectieuses utilisables en ferme
Guyot, Hugues ULg; Ramery, Eve ULg

in Point Vétérinaire (2011), N° Spécial: Maladies Infectieuses des Ruminants: Actualités(314), 78-84

La médecine vétérinaire se développe et s’équipe d’outils de terrain précis pour diagnostiquer la présence d’infections voire même d’en déterminer l’agent étiologique. Des tests de terrain ont été conçus ... [more ▼]

La médecine vétérinaire se développe et s’équipe d’outils de terrain précis pour diagnostiquer la présence d’infections voire même d’en déterminer l’agent étiologique. Des tests de terrain ont été conçus pour pouvoir affiner les diagnostics au chevet du patient. Pour savoir si la maladie d’un bovin examiné a une origine infectieuse, on peu recourir à des tests mesurant l’inflammation, des tests indiquant la présence de cellules inflammatoires, des tests mettant en évidence des agents infectieux au sein de prélèvements biologiques et des tests mesurant les conséquences métaboliques du passage d’un agent infectieux. Par ailleurs, des méthodes de diagnostic de terrain ont été développées pour rechercher de manière spécifique des agents infectieux dans les selles (diarrhées), le lait (mammites), l’urine (infections du système urinaire), le sang (parasites sanguins), et enfin dans les poumons (broncho-pneumonies). Par ailleurs, il est possible de prévoir une morbidité et mortalité accrues chez les jeunes veaux en testant le transfert passif de l’immunité colostrale. La recherche d’un diagnostic étiologique en médecine vétérinaire des ruminants conduit à l’établissement de thérapeutiques ciblées. Il ne faut toutefois pas oublier que les laboratoires d’analyse restent les centres de référence pour confirmer la plupart des étiologies des maladies infectieuses chez les ruminants et autres espèces. [less ▲]

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See detailEffect of a combined iodine and selenium supplementation on I and Se status of cows and their calves
Guyot, Hugues ULg; Alves de Oliveira, Laurent; Ramery, Eve ULg et al

in Journal of Trace Elements in Medicine and Biology (2011), 25(2), 118-124

Iodine (I) and selenium (Se) deficiencies are commonly reported in cattle, however, there are also studies regarding a very high iodine supply. The aim of the study was to determine the long-term effect ... [more ▼]

Iodine (I) and selenium (Se) deficiencies are commonly reported in cattle, however, there are also studies regarding a very high iodine supply. The aim of the study was to determine the long-term effect of I and Se supplementation on non-pregnant cows, pregnant cows and their calves. The hypothalamus pituitary axis was investigated (TSH, T4, T3 assays) during a TRH challenge on non-pregnant cows. Twenty-four cows, half of them pregnant, were assigned into 2 diet-groups, one group with a low I (0.45 ppm) and Se (0.15 ppm) diet (LISe), the other with a high I (5.45 ppm) and Se (0.45 ppm) diet (HISe), for a period of 120 days. Nutritional (plasma iodide, urinary I, plasma Se, I content in colostrum and foetal fluids) and functional (thyrotropin, thyroid hormones, glutathione-peroxidase activity in erythrocytes) markers of I and Se status were assayed in dams at regular intervals for 120 days and in their calves at birth. A TRH challenge was performed on 8 non-pregnant cows at day 110 of the trial. At the end of the study, I and Se nutritional markers were higher in dams in the HISe group, compared to the LISe group, except for plasma Se. At birth, I nutritional markers in calves in the HISe group were higher compared to the LISe group. Reactivity of the pituitary–thyroid-axis was not influenced by I and Se supplementation. I and Se supplementation is efficient in improving newborn status. [less ▲]

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See detailCase Report: Two Cases of Ocular form of Listeriosis in Cattle Herds
Guyot, Hugues ULg

in Cattle Practice (2011), 19(1), 61-64

Nowadays, the widespread use of silage has led to an increased frequency of bacterial contamination of Listeria spp in ruminants. The infection of ruminants is essentially linked to the consumption of ... [more ▼]

Nowadays, the widespread use of silage has led to an increased frequency of bacterial contamination of Listeria spp in ruminants. The infection of ruminants is essentially linked to the consumption of silage of poor quality or of milk products. The disease is frequent and severe in ruminants and can be present in various forms: nervous, septicaemic, genital, mastitis and ocular. The ocular form of Listeriosis can serve as a sentinel for the disease at the herd level. In this case report, 2 herds were investigated for calves and adult mortalities. In the first case, a large majority of the calves presented with hypopion but neurological examination of the calves revealed no abnormalities. The majority of the dairy cows also presented with ocular disorders but neurological examination did not reveal any abnormalities. Calves received whole milk from the dairy herd. The dairy herd diet comprised maize and grass silage, and cereals. Listeria monocytogenes was found in milk and grass silage in significant amounts. In the second case, weight loss, abortions and mortalities in adult cattle were reported. Fifty percent of animals examined presented with hypopion, and/or keratoconjunctivitis, and/or uveitis. No neurological abnormality was found. The animals’ ration of was comprised grass and maize silages. The grass silage had poor macroscopical aspect. Analysis revealed the presence of Schizophyllum commune (fungus) and L. monocytogenes in significant amounts. Furthermore, analysis of aborted foetuses also showed L. monocytogenes. Listeria infection in cattle can occur through ingestion of contaminated silage but calves can also be infected throughout the dams’milk. Ocular lesions are not pathognomonic for Listeriosis but, accompanied with abortions and poor quality silage, this can be a major indication for ancillary exams for Listeria monocytogenes at a herd level. [less ▲]

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See detailSigns of neurotoxicity in a Belgian Blue herd after ingestion of moulded silage
Guyot, Hugues ULg; Sandersen, Charlotte ULg; Brihoum, Mounir ULg et al

in Cattle Practice (2011), 19(1), 57-60

After ingestion of moulded beet pulp silage, cases of cerebro-cortical necrosis (CCN) and mortalities were observed in a dual purpose Belgian Blue (BB) herd. Contamination with Paecilomyces spp., a mould ... [more ▼]

After ingestion of moulded beet pulp silage, cases of cerebro-cortical necrosis (CCN) and mortalities were observed in a dual purpose Belgian Blue (BB) herd. Contamination with Paecilomyces spp., a mould that produces byssochlamic acid, malformins and patulin, was proven. Twenty-five days after progressive introduction of beet pulp silage into the ration, most of the animals showed diminished appetite, excessive salivation and decreased milk production. Some of them showed anorexia, head pressing and blindness while 4 animals died within 1 week after onset of neurological symptoms. The survivors had been treated successfully with thiamine and recovered completely within five days. Once the beet pulp silage had been identified as causative agent, it was removed from the animals’ ration and no more clinical cases were observed. Silage was obviously moulded and analysis revealed the presence of 1.6 million CFU Paecilomyces spp./g of silage. Although no further investigation was undertaken to identify the mycotoxins, intoxication with patulin was suspected, since other mycotoxins produced by these species are less toxic. Although it has not been described that CCN can be induced by ingestion of Paecilomyces spp., it seems that there is a close relation between ingestion of Paecilomyces-contaminated silage and clinical signs observed in this herd. [less ▲]

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See detailTétanos suite à la castration d'un bouc nain
Lamain, Guillaume ULg; Guyot, Hugues ULg

in Point Vétérinaire (2011), 313

Un cas de tétanos faisant suite à la castration d’un bouc nain est décrit. L’animal présente dans un premier temps du tympanisme ruménal, puis de l’opisthotonos. Une neutrophilie est mise en évidence ... [more ▼]

Un cas de tétanos faisant suite à la castration d’un bouc nain est décrit. L’animal présente dans un premier temps du tympanisme ruménal, puis de l’opisthotonos. Une neutrophilie est mise en évidence. L’administration de sérum antitétanique et de pénicilline est accompagnée d’un traitement symptomatique. L’issue de ce cas est fatale et l’autopsie ne révèle pas de lésion spécifique associée au tétanos. [less ▲]

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See detailLa clinique vétérinaire de l'ULg: un partenariat méconnu entre les éleveurs et les vétérinaires
Guyot, Hugues ULg

Article for general public (2011)

La clinique vétérinaire universitaire (CVU) de l'Université de Liège comporte un département s'occupant plus particulièrement de l'Elevage (ruminants et porcs): le DCP (département clinique des animaux de ... [more ▼]

La clinique vétérinaire universitaire (CVU) de l'Université de Liège comporte un département s'occupant plus particulièrement de l'Elevage (ruminants et porcs): le DCP (département clinique des animaux de production). Ce département a des missions d'enseignement, de recherche et de services. Parmi les services, citons la clinique des ruminants (cas référés), les Audits diagnostics et suivis en ferme (ruminants et porcs) et la Clinique Ambulatoire (diagnostic uniquement). [less ▲]

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See detailEmergence of bovine ehrlichiosis in Belgian cattle herds
Guyot, Hugues ULg; Ramery, Eve ULg; O'Grady, Luke et al

in Ticks and Tick-borne Diseases (2011), 2

Bovine ehrlichiosis is a tick-borne rickettsial disease caused by Anaplasma phagocytophilum. The disease can also be transmitted to humans. Outbreaks in cattle have been described in many European ... [more ▼]

Bovine ehrlichiosis is a tick-borne rickettsial disease caused by Anaplasma phagocytophilum. The disease can also be transmitted to humans. Outbreaks in cattle have been described in many European countries. In Belgium, infections caused by Anaplasma phagocytophilum have been reported in humans and dogs; however this paper details the first report of ehrlichiosis in cattle herds in Belgium. The first case described was in a dairy herd located in east Belgium. Clinical signs included hyperthermia, polypnea and swelling of the limbs. The other case was diagnosed in a second, mixed purpose, herd in west Belgium. Within the second herd all of the affected animals came from the same pasture. All animals in that pasture showed recurrent hyperthermia and some also showed signs of mastitis and late-term abortions. Blood smears and serology revealed the presence of Anaplasma phagocytophilum in the majority of animals with pyrexia. Furthermore, the presence of leptospirosis, Neospora caninum and Q fever antibodies was tested by serological analysis but all results were negative. Paired serology for Adenovirus, BHV-4, BHV-1, BVD, PI3 and RSV-B did not show any significant seroconversion. Milk samples from cows affected by mastitis revealed minor pathogens. Fecal testing for the presence of Dictyocaulus viviparus in the first herd was negative. Recurrent pyrexia in pastured cattle is a non-specific sign, and can be related to several different pathogens. Bovine ehrlichiosis is transmitted by the tick species Ixodes ricinus which is known to be present throughout Belgium. Belgian practitioners should include ehrlichiosis in their differential diagnosis when confronted with pastured cattle suffering from recurrent pyrexia. [less ▲]

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See detailLa clinique ambulatoire bovine de l'ULg
Guyot, Hugues ULg

Article for general public (2010)

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See detailEvaluation of a Bovine Concentrated Lactoserum for Preventing Neonatal Diarrhoea in Belgian Blue Calves
Vandeputte, Sébastien ULg; Rollin, Frédéric ULg; Guyot, Hugues ULg et al

in Open Veterinary Science Journal (The) (2010), 4

The purpose of this study was to evaluate, under field conditions, the efficacy of an european registered bovine concentrated lactoserum (Locatim) in 3 farms with neonatal diarrhoea in calves. A total of ... [more ▼]

The purpose of this study was to evaluate, under field conditions, the efficacy of an european registered bovine concentrated lactoserum (Locatim) in 3 farms with neonatal diarrhoea in calves. A total of 117 healthy Belgian Blue (BB) calves were allocated in 2 groups. Two thirds of the calves received Locatim orally immediately after birth and maternal colostrum one hour later (treated group), while control calves only received maternal colostrum. Every day during 14 days, mental status, faeces consistency, suckling reflex and hydration status of each calf were monitored. Individual blood samples were assessed for passive transfer and specific Escherichia coli antibodies against strains F5, CS31A, F17 and F41. Faecal samples from diarrheic and non diarrheic calves were analysed for rotavirus, bovine coronavirus, Cryptosporidium parvum and Escherichia coli F5. Locatim had no significant effect on the onset, duration and incidence of diarrhoea. The mean serum IgG concentration of 23.1 ± 7.8 mg/ml indicates a good IgG transfer. Only the CS31A strain titer was significantly higher in the treated group. The major identified causative agent of diarrhoea was C. parvum. In conclusion, Locatim only has a slight effect when IgG transfer is optimal, but could be justified when specific antibodies lacking in colostrum are needed [less ▲]

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See detailGenomic expression evaluation: introduction and perspectives
Ramery, Eve ULg; Bureau, Fabrice ULg; Guyot, Hugues ULg et al

Conference (2010, July)

In a way, disease could be considered as the result of gene expression deregulation. Knowing what genes are expressed in a particular cell or tissue and how they are regulated allow to better understand ... [more ▼]

In a way, disease could be considered as the result of gene expression deregulation. Knowing what genes are expressed in a particular cell or tissue and how they are regulated allow to better understand disease mechanisms. Transcriptomic is the study of the expression of the entire genome. This technical challenge has been rendered possible as different species’ genomes began to be sequenced. Several techniques have rendered efficient this process of measuring simultaneously the expression level of a large number of genes. To date, micro-array technology is probably the best known. However, with the development of more affordable techniques, sequencing/ resequencing could compete with microarrays in the future. All together, these techniques open new ways for early diagnosis and prognosis for the practioner ‘in the field’. [less ▲]

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See detailLa maladie de Mortellaro: un fléau insidieux dans les élevages bovins
Guyot, Hugues ULg

Article for general public (2009)

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See detailInfection expérimentale de veaux par le virus de la fièvre catarrhale ovine de sérotype 8
Dal Pozzo, Fabiana ULg; De Clercq, K.; Guyot, Hugues ULg et al

in Epidémiologie et Santé Animale (2009), 55

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See detailExperimental reproduction of bluetongue virus serotype 8 clinical disease in calves.
Dal Pozzo, Fabiana ULg; De Clercq, Kris; Guyot, Hugues ULg et al

in Veterinary Microbiology (2009), 136(3-4), 352-8

Cattle are commonly subclinically infected following natural or experimental infection with bluetongue virus (BTV). The introduction of BTV serotype 8 (BTV-8) in Europe has been characterized by the ... [more ▼]

Cattle are commonly subclinically infected following natural or experimental infection with bluetongue virus (BTV). The introduction of BTV serotype 8 (BTV-8) in Europe has been characterized by the manifestation of clinical signs in infected cattle. In order to study the pathogenesis of BTV-8 in this host, an animal model able to reproduce the clinical manifestations of the disease is required. In this work, two calves were subcutaneously and intravenously injected with a low passage cell-adapted strain of BTV-8. Both calves showed typical bluetongue clinical signs, including pyrexia, ocular discharge, conjunctivitis, oral mucosal congestion, development of ulcers and necrotic lesions on the lips and tongue, submandibular oedema, coronitis and oedema of the coronet and pastern region. A score was assigned depending on the severity of the lesions and a total clinical score was calculated for each animal daily and at the end of the experiment. Both calves became viraemic 24h post-infection and seroconversion occurred between 7 and 11 days P.I. In this study we present the development of a protocol of infection in calves able to reproduce the severity of the lesions observed with BTV-8 in field conditions. [less ▲]

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See detailEpidemiology of trace elements deficiencies in Belgian beef and dairy cattle herds.
Guyot, Hugues ULg; Saegerman, Claude ULg; Lebreton, Pascal et al

in Journal of Trace Elements in Medicine and Biology (2009), 23(2), 116-23

Selenium (Se), iodine (I), zinc (Zn) and copper (Cu) deficiencies in cattle have been reported in Europe. These deficiencies are often associated with diseases. The aim of the study was to assess trace ... [more ▼]

Selenium (Se), iodine (I), zinc (Zn) and copper (Cu) deficiencies in cattle have been reported in Europe. These deficiencies are often associated with diseases. The aim of the study was to assess trace element status in Belgian cattle herds showing pathologies and to compare them to healthy cattle herds. Eighty-two beef herds with pathologies, 11 healthy beef herds, 65 dairy herds with pathologies and 20 healthy dairy herds were studied during barn period. Blood and/or milk samples were taken in healthy animals. Plasma Zn, Cu, inorganic I (PII) and activity of glutathione peroxidase in erythrocytes (GPX) were assayed. In milk, I concentration was measured. Data about pathologies and nutrition in the herds were collected. According to defined thresholds, it appeared that a large proportion of deficient herds belonged to "sick" group of herds. This conclusion was supported by the mean value of trace elements and by the fact that a majority of individual values of trace elements was below the threshold. Dairy herds had mean values of trace elements higher than beef herds. More concentrates and minerals were used in healthy herds versus "sick" herds. These feed supplements were also used more often in dairy herds, compared to beef herds. Trace elements deficiencies are present in cattle herds in Belgium and are linked to diseases. Nutrition plays a major role in the trace elements status. [less ▲]

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