References of "Grulke, Sigrid"
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See detailA case of Vitamin K3 induced renal toxicosis in a horse
Sandersen, Charlotte ULg; Cassart, Dominique ULg; Bertrand, P et al

in Tierärztliche Praxis Kleintiere (2004)

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See detailLa parésie spastique du quadriceps fémoral : Une nouvelle entité clinique chez le veau de race Blanc Bleu Belge.
Touati, Kamal ULg; Müller, Philippe ULg; Gangl M. et al

in Annales de Médecine Vétérinaire (2003), 147

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See detailClinicopathological data in equids suffering from ante-hepatic, hepatic and post-hepatic diseases
Amory, Hélène ULg; Perron, MF; Sandersen, Charlotte ULg et al

in Proceedings of the 42th Annual Congress of the British Equine Veterinary Association (BEVA) (2003)

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See detailPrognostic value of clinical signs and blood parameters in equids suffering from acute hepatic insufficiency: a retrospective study on 31 cases
Amory, Hélène ULg; Perron, MF; Sandersen, Charlotte ULg et al

in Proceedings of the 21st ACVIM annual meeting, Charlotte, North Carolina (2003)

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See detailOvero lethal white syndrome - two case reports and a review of the litterature
Sandersen, Charlotte ULg; Delguste, Catherine ULg; Cassart, Dominique ULg et al

in Annales de Médecine Vétérinaire (2003), 147(5, OCT-NOV), 359-364

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See detailEquine trypsin: purification and development of a radio-immunoassay
Grulke, Sigrid ULg; Deby-Dupont, G.; Gangl, M. et al

in Veterinary Research (2003), 34(3, May-Jun), 317-330

Shock is accompanied by generalised splanchnic hypoperfusion, and splanchnic organs like the pancreas can be damaged, as shown in animal experimental models and in humans, by the presence of high plasma ... [more ▼]

Shock is accompanied by generalised splanchnic hypoperfusion, and splanchnic organs like the pancreas can be damaged, as shown in animal experimental models and in humans, by the presence of high plasma concentrations of trypsin and other pancreatic enzymes. In order to design a radioimmunoassay technique (RIA) for the measurement of equine trypsin-like immunoreactivity (TLI) in biological fluids, trypsin was purified (with purity greater than or equal to 96 %) from the equine pancreas by extraction in an acid medium, ammonium sulfate precipitations, gel filtration chromatography and, after activation of trypsinogen into trypsin, affinity chromatography. Gel polyacrylamide electrophoresis showed a monomeric enzyme with a molecular weight of 27 kDa. The purified equine trypsin served for the immunisation of rabbits in order to obtain a specific antiserum, and the labelled antigen was prepared by iodination of equine trypsin with I-125. The RIA was based on the binding of the antigen to the antibody followed by the separation of the antigen-antibody complex by immunoprecipitation in the presence of sheep anti-rabbit gammaglobulins and the assay of the radioactivity in the precipitate. The RIA showed good sensitivity, specificity, precision, accuracy and reproducibility. The reference mean value of TLI in the plasma of healthy horses (n = 20) was 30.01 +/- 6.84 ng/mL (upper confidence limit 50.52 ng/mL; p < 0.01). Three horses with non strangulating intestinal obstruction without shock showed TLI values within normal limits whereas 5 of 7 horses with strangulation obstruction showed TLI levels above the upper confidence limit. Further studies using the RIA and the enzymatic assay should be performed in order to confirm the role of the pancreas in equine intestinal obstruction. [less ▲]

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See detailPancreatic damage observed in two horses euthanized for persistent paralytic ileus.
Grulke, Sigrid ULg; Deby, Ginette ULg; Gangl, Monika et al

Poster (2002, July)

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See detailPeritoneal trypsin level in equine acute abdominal disease.
Grulke, Sigrid ULg; Gueret, Patricia; Gangl, Monika et al

Conference (2002, July)

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See detailPlasma trypsin level in horses suffering from acute intestinal obstruction
Grulke, Sigrid ULg; Gangl, M.; Deby, Ginette ULg et al

in Veterinary Journal (2002), 163(3), 283-291

Gastrointestinal disorders in horses leading to endotoxic shock could have further consequences on other splanchnic organs such as the pancreas, as can be seen in humans suffering from septic shock. In ... [more ▼]

Gastrointestinal disorders in horses leading to endotoxic shock could have further consequences on other splanchnic organs such as the pancreas, as can be seen in humans suffering from septic shock. In this study, the range of enzymatically active trypsin (EAT) in healthy horses was established and is similar to the range observed in healthy humans. EAT values were determined in horses with acute abdominal crises on admission as well as during anaesthesia and in the postoperative phase. A significant increase in plasma EAT was found in 59% of the horses with surgical colic when compared to our established reference range. Significantly higher values were found in severe shock cases. When separated in groups according to the duration of colic before referral, significantly higher EAT values were observed in the non-survivor group compared to the survivor group of colics of short duration. EAT plasma values increased significantly during the postoperative phase, and were significantly higher in small intestine obstructions than in large bowel disorders. In human medicine, hypovolaemic or septic shock patients show an increase in pancreatic proteases. Splanchnic hypoperfusion during shock could lead to pancreatic damage resulting in trypsin liberation into the peritoneal space and an increase in plasma levels. Trypsin is able to activate inflammatory cascades and leucocytes and could play a role in multiple organ failure. Further studies are needed to evaluate the implications of changes in plasma trypsin in the disease process of equine acute abdomen and to demonstrate possible pancreatic damage. [less ▲]

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See detailApproche clinique des ictères et des suspicions d’insuffisance hépatique aiguë chez le cheval : une étude rétrospective sur 40 cas
Amory, Hélène ULg; Sandersen, Charlotte ULg; Delguste, Catherine ULg et al

in Proceedings of the Annual meeting of the French Equine Veterinary Association (AVEF) (2002)

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See detailPlasma trypsin level increase in horses during post-operative period after colic surgery.
Grulke, Sigrid ULg; Gangl, Monika; Deby, Ginette ULg et al

Conference (2001, October)

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See detailDetermination of a gravity and shock score for prognosis in equine surgical colic
Grulke, Sigrid ULg; Olle, E.; Detilleux, Johann ULg et al

in Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine (2001), 48(8), 465-73

A retrospective study evaluated 200 surgical colic cases. A gravity score (GS) based on four clinical parameters estimating intestinal obstruction (rectal palpation, borborygmi, abdominal distension, pain ... [more ▼]

A retrospective study evaluated 200 surgical colic cases. A gravity score (GS) based on four clinical parameters estimating intestinal obstruction (rectal palpation, borborygmi, abdominal distension, pain) and classified into three categories was established and tested to determine if it could evaluate prognosis. A shock score (SS) based on six parameters was also attributed to each case. The overall survival rate was 54%. The statistical analysis showed a significant (P < 0.01) difference in the survival rate in the different categories of the GS, as well as in the categories of the SS. A multivariate logistic regression model showed that horses with GS 3 are 10.6 times more likely to die than those with GS 1. A model combining the two scores showed an odds ratio of 7.1 for GS 3 versus GS 1, and for SS 3 versus SS 1, the odds ratio was 7.2. [less ▲]

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See detailRetrospective study of 206 cases of surgical intestinal pathologies in the horse : incidence, diagnosis, prognosis and complications.
Grulke, Sigrid ULg; Gangl, Monika; Caudron, Isabelle et al

in Annales de Médecine Vétérinaire (2000), 144

This retrospective study of 206 surgical colic cases shows the incidence of the different types of pathologies. The small intestine is concerned in 106 (51,5 %) cases with 102 (96 %) strangulation ... [more ▼]

This retrospective study of 206 surgical colic cases shows the incidence of the different types of pathologies. The small intestine is concerned in 106 (51,5 %) cases with 102 (96 %) strangulation obstructions. The remaining 100 cases (48,5 %) are large intestine obstructions with 45 (45 %) strangulation obstructions. The global survival rate is of 51,5 %, of 42,2 % for small intestine and of 61 % for large intestine. A gravity and a shock score permit to define the prognosis before the decision for surgery. The incidence of the postoperative complications is higher (55,2 %) and the time for recovery is significantly longer for small intestinal pathologies than for large intestine affections. Shock and paralytic ileus are the most frequent complications accounting for 65 % of post operative mortalities. The survival rates are significantly different according to gravity and shock score. The time of recovery is also significantly different in function of the shock score. These findings show the importance of rapid diagnosis of the acute abdominal disease in order to improve the outcome. [less ▲]

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See detailGravity Score and Shock Score as an Aid to dertemine the prognosis in Equine Surgical Colic Cases
Grulke, Sigrid ULg; Olle, Enrice; Detilleux, Johann ULg et al

in Veterinary Surgery : The Official Journal of the American College of Veterinary Surgeons (1999, July)

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See detailPlasma Myeloperoxidase Level and Polymorphonuclear Leukocyte Activation in Horses Suffering from Large Intestinal Obstruction Requiring Surgery: Preliminary Results
Grulke, Sigrid ULg; Benbarek, Hama; Caudron, I. et al

in Canadian Journal of Veterinary Research = Revue Canadienne de Recherche Vétérinaire (1999), 63(2), 142-7

Myeloperoxidase (MPO) is a specific enzyme of neutrophil azurophilic granules with a strong oxidative activity. Thanks to a radioimmunoassay of equine myeloperoxidase, the authors have observed a ... [more ▼]

Myeloperoxidase (MPO) is a specific enzyme of neutrophil azurophilic granules with a strong oxidative activity. Thanks to a radioimmunoassay of equine myeloperoxidase, the authors have observed a significantly higher plasma level of MPO in horses operated for strangulation obstruction of the large intestine (n = 6) than in horses suffering from a non-strangulating displacement of the large intestine (n = 9). For the 2 groups, 3 phases were distinguished: reception (P1), intensive care (P2) and terminal phase (P3). The mean peak values of MPO for these phases were 121.6 ng/mL (P1), 168.6 ng/mL (P2), and 107.0 ng/mL (P3) for the non-strangulating group, and 242.6 ng/mL (P1); 426.0 ng/mL (P2), and 379.5 ng/mL (P3) for the strangulation group. The variations of the mean peak values of plasma MPO were significantly different between the 2 groups and between the different phases. A significant increase of the least square means of MPO was observed between P1 and P2. A significant decrease of the least square means of the number of circulating leukocytes was observed between P1 and P3. Polymorphonuclear neutrophil activation could play a major role in the pathogenesis of acute abdominal disease and endotoxic shock. [less ▲]

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