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See detailQuantification of Randomly-methylated-b-cyclodextrin effect on liposome: An ESR study
Grammenos, Angeliki ULg; Bahri, Mohamed Ali ULg; Guelluy, Pierre-Henri ULg et al

in Biochemical and Biophysical Research Communications (2009), 390

In the present work, the effect of Randomly-methylated-b-cyclodextrin (Rameb) on the microviscosity of dimyristoyl-L-a phosphatidylcholine (DMPC) bilayer was investigated using the electron spin resonance ... [more ▼]

In the present work, the effect of Randomly-methylated-b-cyclodextrin (Rameb) on the microviscosity of dimyristoyl-L-a phosphatidylcholine (DMPC) bilayer was investigated using the electron spin resonance (ESR) technique. The ability of Rameb to extract membrane cholesterol was demonstrated. For the first time, the percentage of cholesterol extracted by Rameb from cholesterol doped DMPC bilayer was monitored and quantified throughout a wide Rameb concentration range. The effect of cholesterol on the inner part of the membrane was also investigated using 16-doxyl stearic acid spin label (16-DSA). 16-DSA seems to explore two different membrane domains and report their respective microviscosities. ESR experiments also establish that the presence of 30% of cholesterol in DMPC liposomes suppresses the jump in membrane fluidity at lipids phase-transition temperature (23.9°C). [less ▲]

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See detailInvestigation of SDS, DTAB and CTAB micelle microviscosities by electron spin resonance
Bahri, Mohamed Ali ULg; Hoebeke, Maryse ULg; Grammenos, Angeliki ULg et al

in Colloids and Surfaces A : Physicochemical and Engineering Aspects (2006), 290(1-3), 206-212

Electron spin resonance spectroscopy (ESR) of the nitroxide labelled fatty acid probes (5-, 16-doxyl stearic acid) was used to monitor the micelle microviscosity of three surfactants at various ... [more ▼]

Electron spin resonance spectroscopy (ESR) of the nitroxide labelled fatty acid probes (5-, 16-doxyl stearic acid) was used to monitor the micelle microviscosity of three surfactants at various concentrations in aqueous solution: sodium dodecyl sulphate (SDS), dodecyltrimethylammonium bromide (DTAB) and cetyltrimethylammonium bromide (CTAB). At low surfactant concentration, there is no micelle, the ESR probe is dissolved in water/surfactant homogeneous phase and gives his microviscosity. At higher surfactant concentration, an abrupt increase in microviscosity indicates the apparition of micelles and, the solubilization of the probes in micelles. The microviscosity of the three surfactants, in a large surfactant range, was obtained as well as the critical micelle concentration (CMC). The microviscosity increased slightly with the increase in surfactant concentration. Phosphate buffer lowered the CMC value and generally increased the microviscosity. (c) 2006 Elsevier B.V. All rights reserved. [less ▲]

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