References of "Goessens, Guy"
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See detailUltrastructural Cytochemistry of the Nucleolus in Rat Oocytes at the End of the Folliculogenesis
Antoine, Nadine ULg; Lepoint, A.; Baeckeland, Edgard ULg et al

in Histochemistry (1988), 89(3), 221-6

Various ultrastructural changes occur during follicular growth in the rat oocyte nucleolus. The nucleolus, which has a reticulated fibrillogranular structure at the primordial and primary follicle stages ... [more ▼]

Various ultrastructural changes occur during follicular growth in the rat oocyte nucleolus. The nucleolus, which has a reticulated fibrillogranular structure at the primordial and primary follicle stages, becomes entirely compact and is made up of a conspicuous and homogeneous mass at the antral follicle stage. In order to define the nature and the functions of this homogeneous mass, cytochemical methods allowing detection of nucleic acids, proteins and lipids were performed at the light microscopic and ultrastructural levels. The results obtained suggest that this nucleolar mass is probably composed of acid proteins which are not silver stained. This proteinaceous mass could be a special kind of nucleolar secretion providing material for meiotic resumption in the oocyte. Cytochemical researches now in progress should supply new information concerning the exact nature and the role of the nucleolar compact mass, which is the essential nucleolar component at the antral follicle stage and which really plays a role in the nucleolus in the first stages of embryogenesis. [less ▲]

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See detailUltrastructural and cytochemical study on nucleolus in rat oocytes using RNase gold complex
Antoine, Nadine ULg; Thiry, Marc ULg; Baeckeland, E et al

in Biology of the Cell (1987), 60

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See detailAnti SSA and anti SSB autoantibodies: an immunofluorescence, ultrastructural and immunoblotting study
Thiry, Marc ULg; Vigneron, Alain; Humbel, René et al

Poster (1987)

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See detailLe nucléole : un organite cellulaire fondamental
Lepoint, Alain; Goessens, Guy ULg; Thiry, Marc ULg

in Revue de l'Association Belge des Technologies de Laboratoire (1987), 14

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See detailRelations between nucleoli and nucleolus-organizing regions during the cell cycle
Goessens, Guy ULg; Thiry, Marc ULg; Lepoint, Alain

in Chromosomes Today (1987), 9

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See detailBehaviour of nucleolus during mitosis. A comparative ultrastructural study of various cancerous cell lines using the Ag-NOR staining procedure.
Ploton, D.; Thiry, Marc ULg; Menager, M. et al

in Chromosoma (1987), 95(2), 95-107

The aim of the present work was to study the distribution and the behaviour of the silver-staining nucleolar organizer region (Ag-NOR) proteins at the ultrastructural level during interphase and mitosis ... [more ▼]

The aim of the present work was to study the distribution and the behaviour of the silver-staining nucleolar organizer region (Ag-NOR) proteins at the ultrastructural level during interphase and mitosis in five human and murine cancerous cell lines each characterized by a typical nucleolar morphology. During interphase the Ag-NOR proteins are restricted to the fibrillar centres (F.C.) and/or to the dense fibrillar component (D.F.C.). During prophase the silver-staining components come into close contact with some chromosomes and are arranged with a typical polarity: chromosome, F.C. and D.F.C. Then F.C. and D.F.C. together form roundish silver-stained structures and integrate in part within indentations at the periphery of the metaphase chromosomes. During anaphase and telophase large and small spherical silver-staining structures may be seen. They correspond respectively to the metaphase NORs and to numerous structures which appear de novo within ribonucleoprotein (RNP) material localized between the chromosomes. During late telophase the number of the small silver-staining structures decreases whereas the size of the larger ones increases. Then the interphase nucleoli recover their typical shape. These results suggest that when rRNA synthesis is impaired during mitosis the inactive NORs assume a structure and a localization which are not typical of the cell line. In contrast the F.C. and D.F.C. are probably two aspects of the NORs whose typical distribution, relative to the other nucleolar components, gives the interphasic nucleolus its characteristic morphology. [less ▲]

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See detailEssais de localisation des RNA polymérases dans des cellules tumorales d'Ehrlich par immunocytochimie ultrastructurale
Thiry, Marc ULg; Hendrick, J C; Lepoint, Alain et al

in Biology of the Cell (1986), 57

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See detailEtude immunocytochimique ultrastructurale de cellules tumorales d'Ehrlich à l'aide d'anticorps dirigés contre la RNA polymérase d'E. Coli
Thiry, Marc ULg; Hendrick, J C; Lepoint, Alain et al

in Bulletin de l'Association des Anatomistes (1986), 70

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See detailAntinucleolar autoantibodies: ultrastructural localization of antigens on HEp 2 cells
Thiry, Marc ULg; Vigneron, Alain; Humbel, René L et al

Poster (1986)

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See detailUltrastructural localization of antigens on HEp 2 cells using antinucleolar autoantibodies
Thiry, Marc ULg; Vigneron, Alain; Humbel, René L et al

in Immunobiology (1986), 173

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See detailUltrastructure of HEp 2 cells
Thiry, Marc ULg; Humbel, R; Goessens, Guy ULg

in Peeters, H (Ed.) Protides of the Biological Fluids (1986)

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See detailUltrastructural study of the relationships between the various nucleolar components in Ehrlich tumour and HEp-2 cell nucleoli after acetylation.
Thiry, Marc ULg; Goessens, Guy ULg

in Experimental Cell Research (1986), 164(1), 232-42

In the present study, we analysed the relationships between various nucleolar components in Ehrlich tumour and HEp-2 cells, using acetylation. Under these conditions, we found contacts between the ... [more ▼]

In the present study, we analysed the relationships between various nucleolar components in Ehrlich tumour and HEp-2 cells, using acetylation. Under these conditions, we found contacts between the condensed intranucleolar chromatin and the fibrillar centre, illustrating the continuity between the DNA present inside the fibrillar centre and that of condensed associated chromatin. We also found that although the dense fibrillar component is usually situated at the periphery of the fibrillar centre, it is sometimes found inside the centre. On the other hand, the layer of dense fibrils bordering the fibrillar centre is interrupted by nucleolar interstices. In addition, in HEp-2 cell nucleoli with a reticulated appearance, the numerous small fibrillar centres are bound together by strands of dense fibrillar component. These observations are discussed in terms of relationships between nucleolar ultrastructure and function(s). [less ▲]

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See detailAntinucleolar antibodies: ultrastructural localisation of the antigens
Humbel, René L; Thiry, Marc ULg; Goessens, Guy ULg et al

Poster (1985)

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See detailRe-evaluation of the site of transcription in Ehrlich tumour cell nucleoli.
Thiry, Marc ULg; Lepoint, A.; Goessens, Guy ULg

in Biology of the Cell (1985), 54(1), 57-64

Recent electron microscopic immunolocalization studies (Scheer and Rose, 1984) indicated that RNA polymerase I is located in, and probably confined to, the fibrillar centres of the nucleoli. This ... [more ▼]

Recent electron microscopic immunolocalization studies (Scheer and Rose, 1984) indicated that RNA polymerase I is located in, and probably confined to, the fibrillar centres of the nucleoli. This conclusion appears to be in contradiction to previous autoradiographic studies reporting that transcription of rRNA genes takes place in the dense fibrillar component. Despite the fact that rRNA transcription occurs in the dense fibrillar component of Ehrlich tumour cell nucleoli, we show in the present paper that dense fibrillar component containing rapidly labelled RNA can be visualized not only around but also inside the fibrillar centres. [less ▲]

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See detailVisualization of the nucleolar components after acetylation
Thiry, Marc ULg; Lepoint, Alain; Goessens, Guy ULg

Poster (1984)

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