Reevaluation by Image Analysis of the Effects of Fibroblasts, Fibronectin or Laminin Upon Colony Formation in Mouse Lungs by B16 Melanoma Cells

in Invasion & Metastasis (1993), 13(4), 201-11

A recently described personal method based on image analysis of histological sections was used in order to quantify lung colony formation by B16 melanoma cells injected intravenously into the mouse. These ... [more ▼]

A recently described personal method based on image analysis of histological sections was used in order to quantify lung colony formation by B16 melanoma cells injected intravenously into the mouse. These tumor cells were preincubated in vitro either with fibronectin (FN), laminin (LN) or fibroblasts (FB), which are implicated in the process of invasion and metastasis. Thanks to this method, a more accurate analysis of lung colonies (section area and number) formed by tumor cells was realized. By image analysis, we show that when FB were mixed with B16 cells, a drastic increase of tumor sections number and area was induced. LN increased the tumor sections area, but not their number. No effect of FN on B16 cells was observed. LN and FN promoted tumor anchorage in the depth of the lungs while FB reduced the latter. These facts could explain the contradictory results obtained by simply counting macroscopically superficial lung colonies. When cultured in vitro, these B16 melanoma cells did not produce any type of IV collagenase, either alone or in the presence of LN or FN, but in cocultures (B16 with 3T3) and in fibroblasts cultures, this enzyme was present. This could explain, among other factors, why the rate of invasiveness exerted by B16 cells is higher when the latter are coinjected with FB. [less ▲]

Laminin and 67 Kd Laminin Binding Protein in Mouse B16 Melanoma Cells and 3t3 Fibroblast Spheroids

in Anticancer Research (1992), 12(6B, Nov-Dec), 2011-6

Multicellular spheroids which promote cell-cell and cell-matrix interactions were prepared in culture with mouse B16 melanoma cells (pigmented or non pigmented) alone or mixed with mouse 3T3 fibroblasts ... [more ▼]

Multicellular spheroids which promote cell-cell and cell-matrix interactions were prepared in culture with mouse B16 melanoma cells (pigmented or non pigmented) alone or mixed with mouse 3T3 fibroblasts. Their volume and proliferation or necrosis rate were evaluated. As measured by dot blot immunoassay, laminin was mainly produced by fibroblasts rather than by melanoma cells. High levels of laminin B1 chain mRNA were detected only in spheroids composed of 3T3 fibroblasts. The levels of 67 kD laminin binding protein mRNA were high in all cell populations studied here. [less ▲]

Cytological effects of culture media conditioned B16 melanoma cells and 3T3 fibroblasts

in Anticancer Research (1991), 11(2), 801-804

Cytotoxic soluble fractions (M.W.<1,000) were prepared from media conditioned by mixed cultures of 3T3 fibroblasts and B16 cells. The ultrastructural analyses of cells (B16 or 3T3) treated with these ... [more ▼]

Cytotoxic soluble fractions (M.W.<1,000) were prepared from media conditioned by mixed cultures of 3T3 fibroblasts and B16 cells. The ultrastructural analyses of cells (B16 or 3T3) treated with these fractions revealed in them mitochondria swelling, blebs, broken membranes and dead cells. [less ▲]

Effects of Strychnopentamine on Cells Cultured in Vitro

in Chemico-Biological Interactions (1991), 80(2), 203-16

This paper describes the powerful cytotoxic action exerted by strychnopentamine (SP), a dimeric indole alkaloid extracted from Strychnos usambarensis Gilg, on B16 melanoma cells and on non-cancer human ... [more ▼]

This paper describes the powerful cytotoxic action exerted by strychnopentamine (SP), a dimeric indole alkaloid extracted from Strychnos usambarensis Gilg, on B16 melanoma cells and on non-cancer human fibroblasts cultured in vitro. SP strongly inhibits cell proliferation and induces cell death at a relatively low concentration (less than 1 microgram/ml) after 72 h of treatment in the two lines. Incorporation of [3H]thymidine and [3H]leucine by B16 cells significantly decreases after only 1 h of treatment at 0.5 microgram/ml. SP induces the formation of dense lamellar bodies and vacuolization in the cytoplasm, intense blebbing at the cell surface and various cytological alterations leading to cell death. [less ▲]

Effects of FeSO4 on B16 melanoma cells differentiation and proliferation

in Anticancer Research (1990), 10(4), 1029-1033

The paper shows that FeS04 can increase or reduce B16 cell proliferation and melanogenesis. Vitamin C toxicity for B16 cells was reduced in the presence of FeSO4.

Toxic and mitogenic activities of culture media conditioned by B16 melanoma cells

Conference (1990)

Analysis of Tridimensional Mixed Cultures of Mouse B16 Melanoma Cells and 3t3 Fibroblasts

in Anticancer Research (1988), 8(1, Jan-Feb), 153-60

The interactions between invasive malignant cells and normal fibroblastic cells were studied in a cellular spheroid model in vitro. Murine B16 melanoma cells (previously cultured in monolayer for a short ... [more ▼]

The interactions between invasive malignant cells and normal fibroblastic cells were studied in a cellular spheroid model in vitro. Murine B16 melanoma cells (previously cultured in monolayer for a short period) and 3T3 mouse fibroblasts (greater than or equal to 130 passages in monolayers) were cultured under tridimensional conditions (pure or mixed spheroids). As compared to pure 3T3 or mixed spheroids, B16 spheroids were smaller and characterized by a higher proliferation rate, a lower degree of necrosis, and a less abundant extracellular matrix. Disintegration was observed in some pure 3T3 or mixed spheroids. Melanogenesis progressively increased inside B16 or mixed spheroids. By immunohistochemical methods and electron microscopy, laminin, fibronectin and collagen I, III and IV in extracellular matrix were studied in the three types of spheroids. [less ▲]

Effects of copper sulfate given alone or with alpha-MSH and L-tyrosine on B16 melanoma cells cultured in serum-free media.

in Archives of Biology (1987), 98(3), 327-39

This study shows that copper sulphate (0.1mM) has antiproliferative effects in B16 melanoma cells cutured in various serum-free media.

Etude de la matrice extracellulaire dans des sphéroïdes multi-cellulaires de mélanome B16 en culture

Poster (1986, May)

Co-cultures of mouse B16 melanoma cells and 3T3 fibroblasts in three dimensions

in European Journal of Cell Biology. Supplement (1986), 42(Suppl 15), 49