References of "Gillet, Marie-Claire"
     in
Bookmark and Share    
See detailInduction of apoptosis in HL-60 by a trachylobane diterpene from Croton Zambesicus
Block, Sébastien; Gillet, Marie-Claire ULg; Gerkens, Pascal et al

Poster (2002, September)

Detailed reference viewed: 5 (0 ULg)
Full Text
Peer Reviewed
See detailApoptosis of HL-60 leukemia cells induced by the bisindole alkaloids sungucine and isosungucine from Strychnos icaja
Lansiaux, A.; Bailly, Christian; Houssier, Claude ULg et al

in Planta Medica (2002), 68(7), 591-595

Sungucine and isosungucine are two bisindole alkaloids isolated from the roots of the African plant Strychnos icaja Baillon. They both exhibit antiplasmodial activities but also show cytotoxic effects ... [more ▼]

Sungucine and isosungucine are two bisindole alkaloids isolated from the roots of the African plant Strychnos icaja Baillon. They both exhibit antiplasmodial activities but also show cytotoxic effects against human cancer cell lines. In order to elucidate their mechanism of action, we have investigated the interaction of the alkaloids with DNA and their capacity to inhibit nucleic acids and protein synthesis in the human HL-60 promyelocytic leukemia cell line. Cell treatment with both sungucine and isosungucine leads to the appearance of a hypo-diploid DNA content peak. Western blotting analysis reveals that the two alkaloids induce cleavage of the poly(ADP-ribose) polymerase (PARP) and promote the cleavage of a caspase-3 DEVD peptide substrate. The activation of the caspase cascade is accompanied with a fragmentation of DNA in cells, as revealed by the TUNEL assay. Altogether, the results shed light on the mechanism of action of these two plant alkaloids and identify signaling factors involved in (iso)sungucine-induced apoptosis in HL-60 cells. [less ▲]

Detailed reference viewed: 51 (1 ULg)
See detailSearch for apoptotic compounds in Croton zambesicus
Mavar-Manga, H; Block, Sébastien; Gillet, Marie-Claire ULg et al

Conference (2002, March)

Detailed reference viewed: 8 (0 ULg)
See detailContribution to the study of Alchornea cordifolia (Schumach. & Thonn.)
Mavar-Manga, H; Brkic, D; Gillet, Marie-Claire ULg et al

Conference (2002, March)

Detailed reference viewed: 8 (0 ULg)
See detailAspects quantitatifs de la spectrométrie de masse des biomolécules
De Pauw, Edwin ULg; Gabelica, Valérie ULg; Gillet, Marie-Claire ULg et al

Conference (2002, March)

Spectrométrie de masse en 2002 : plus haut, plus vite… mais jusqu’où et pourquoi faire ?

Detailed reference viewed: 5 (1 ULg)
Full Text
Peer Reviewed
See detailEnt-Trachyloban-3beta-ol, a new cytotoxic diterpene frm Croton zambesicus
Block, Sebastien; Stevigny, Caroline; Gillet, Marie-Claire ULg et al

in Planta Medica (2002), 68

The dichloromethane extract of leaves of Croton Zambesicus (Euphorbiaceae) showing in vitro cytotoxicity against huma cervix carcinoma cells was investigated in order to identify its active compounds. A ... [more ▼]

The dichloromethane extract of leaves of Croton Zambesicus (Euphorbiaceae) showing in vitro cytotoxicity against huma cervix carcinoma cells was investigated in order to identify its active compounds. A bio-guided fractionation by HSCCC followed by MPLC led us to isolate a trachylobane diterpene, ent-trachyloban-3beta-ol, with cytotoxic properties (IC50 on HeLa cells = 7.3 ùg/ml). This is the first report on the cytotoxicity of a trachylobane diterpene. [less ▲]

Detailed reference viewed: 21 (1 ULg)
Full Text
Peer Reviewed
See detailStrychnogucines a and B, Two New Antiplasmodial Bisindole Alkaloids from Strychnos Icaja
Frederich, Michel ULg; Gillet, Marie-Claire ULg; Prosperi, Christelle ULg et al

in Journal of Natural Products (2001), 64(1), 12-16

A reinvestigation of Strychnos icaja roots has resulted in the isolation of two tertiary quasi-symmetric bisindole alkaloids named strychnogucines A (1) and B (2). Their structures were identified by ... [more ▼]

A reinvestigation of Strychnos icaja roots has resulted in the isolation of two tertiary quasi-symmetric bisindole alkaloids named strychnogucines A (1) and B (2). Their structures were identified by means of spectroscopic data interpretation. Compound 2 was highly active in vitro and compound 1 moderately active against four strains of Plasmodium falciparum. Strychnogucine B (2) was more active against a chloroquine-resistant strain than against a chloroquine-sensitive one (best CI(50), 80 nM against the W2 strain). In addition, this compound showed a selective antiplasmodial activity with 25-180 times greater toxicity toward P. falciparum, relative to cultured human cancer cells (KB) or human fibroblasts (WI38). [less ▲]

Detailed reference viewed: 51 (7 ULg)
See detailCytotoxic aporphine alkaloids from Cassytha filiformis L
Stévigny, caroline; Gillet, Marie-Claire ULg; de Hoffmann, E et al

Poster (2001)

Detailed reference viewed: 20 (0 ULg)
See detailEvaluation of the antitrypanosomal activity of several plants traditionally used in Benin to treat sleeping sickness
Hoet, Sarah; Cottem, D; Opperdoes, F et al

Poster (2001)

Detailed reference viewed: 4 (0 ULg)
Full Text
Peer Reviewed
See detailDNA intercalation, topoisomerase II inhibition and cytotoxic activity of the plant alkaloid cryptolepine
Bailly, Christian; Laine, W.; Baldeyrou, B. et al

in Anti-Cancer Drug Design (2000), 15(3), 191-201

Cryptolepine and neocryptolepine are two indoloquinoline alkaloids isolated from the roots of the African plant Cryptolepis sanguinolenta. Both drugs have revealed antibacterial and antiparasitic ... [more ▼]

Cryptolepine and neocryptolepine are two indoloquinoline alkaloids isolated from the roots of the African plant Cryptolepis sanguinolenta. Both drugs have revealed antibacterial and antiparasitic activities and are strongly cytotoxic to tumour cells. We have recently shown that cryptolepine can intercalate into DNA and stimulates DNA cleavage by human topoisomerase II. In this study, we have investigated the mechanism of action and cytotoxicity of neocryptolepine, which differs from the parent isomer only by the orientation of the indole unit with respect to the quinoline moiety. The biochemical and physicochemical results presented here indicate that neocryptolepine also intercalates into DNA, preferentially at GC-rich sequences, but exhibits a reduced affinity for DNA compared with cryptolepine. The two alkaloids interfere with the catalytic activity of human topoisomerase II but the poisoning activity is slightly more pronounced with cryptolepine than with its isomer. The data provide a molecular basis to account for the reduced cytotoxicity of neocryptolepine compared with the parent drug. [less ▲]

Detailed reference viewed: 51 (2 ULg)
Full Text
Peer Reviewed
See detailNew Antimalarial and Cytotoxic Sungucine Derivatives from Strychnos Icaja Roots
Frederich, Michel ULg; Gillet, Marie-Claire ULg; Llabres, Gabriel ULg et al

in Planta Medica (2000), 66(3), 262-9

Reinvestigation of Strychnos icaja Baillon resulted in the isolation of vomicine, isostrychnine and of three new sungucine derivatives, named isosungucine (8), 18-hydroxy-sungucine (9) and 18-hydroxy ... [more ▼]

Reinvestigation of Strychnos icaja Baillon resulted in the isolation of vomicine, isostrychnine and of three new sungucine derivatives, named isosungucine (8), 18-hydroxy-sungucine (9) and 18-hydroxy-isosungucine (10). They were identified by detailed spectroscopic methods. The complete 1H- and 13C-NMR study of sungucine was also realized. Some of these compounds were highly active against Plasmodium falciparum in vitro and more particularly against the chloroquine-resistant strain. Compound 10 showed a selective antiplasmodial activity, with > 100-fold greater toxicity towards Plasmodium falciparum, relative to cultured human cancer cells (KB and HeLa lines) or fibroblasts (WI38). [less ▲]

Detailed reference viewed: 53 (13 ULg)
Full Text
Peer Reviewed
See detailEffect of nicotine on rat gingival fibroblasts in vitro
Lahmouzi, Jamila ULg; Simain-Sato, Franklin ULg; Defresne, Marie-Paule ULg et al

in Connective Tissue Research (2000), 41(1), 69-80

Nicotine from 3 to 5 mM affects growth and survival rate of rat gingival fibroblasts in vitro. Ultrastructural analysis revealed dilated mitochondria and vacuolization in treated cells, suggestive of ... [more ▼]

Nicotine from 3 to 5 mM affects growth and survival rate of rat gingival fibroblasts in vitro. Ultrastructural analysis revealed dilated mitochondria and vacuolization in treated cells, suggestive of necrosis, but increased apoptosis was also revealed by cytometry. On the basis of this in vitro study, it appear that tobacco, through its component nicotine, may directly affect various functions of rat gingival fibroblasts [less ▲]

Detailed reference viewed: 116 (9 ULg)
See detailLysosomal phospholipases inhibition by emetine, strychnopentamine and usambarensine
Quetin-Leclercq, Joelle; Schomer, G; Van Bambeke, F et al

Poster (1999)

Detailed reference viewed: 4 (0 ULg)