References of "Geris, Liesbet"
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See detailA mathematical model of calcium ion influence on the activity of osteogenic cells
Carlier, Aurélie ULg; Chai, Yoke Chin; Theys, Tina et al

Poster (2010, November 19)

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See detailA mathematical model of calcium ion influence on the activity of osteogenic cells
Carlier, Aurélie ULg; Chai, Yoke Chin; Theys, Tina et al

Poster (2010, November 19)

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See detailA Boolean network model of the growth plate
Kerkhofs, Johan ULg; Roberts, Scott J; Luyten, Frank P et al

Poster (2010, October 10)

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See detailBMP signalling in growth plate chondrocytes: a Boolean modelling approach
Kerkhofs, Johan ULg; Roberts, Scott J; Van Oosterwyck, Hans et al

Poster (2010, September 15)

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See detailOccurrence and Treatment of Bone Atrophic Non-Unions Investigated by an Integrative Approach
Geris, Liesbet ULg; Reed, Anita A.C.; Vander Sloten, Jos et al

in PLoS Computational Biology (2010), 6(9), 1000915

Recently developed atrophic non-union models are a good representation of the clinical situation in which many nonunions develop. Based on previous experimental studies with these atrophic non-union ... [more ▼]

Recently developed atrophic non-union models are a good representation of the clinical situation in which many nonunions develop. Based on previous experimental studies with these atrophic non-union models, it was hypothesized that in order to obtain successful fracture healing, blood vessels, growth factors, and (proliferative) precursor cells all need to be present in the callus at the same time. This study uses a combined in vivo-in silico approach to investigate these different aspects (vasculature, growth factors, cell proliferation). The mathematical model, initially developed for the study of normal fracture healing, is able to capture essential aspects of the in vivo atrophic non-union model despite a number of deviations that are mainly due to simplifications in the in silico model. The mathematical model is subsequently used to test possible treatment strategies for atrophic non-unions (i.e. cell transplant at post-osteotomy, week 3). Preliminary in vivo experiments corroborate the numerical predictions. Finally, the mathematical model is applied to explain experimental observations and identify potentially crucial steps in the treatments and can thereby be used to optimize experimental and clinical studies in this area. This study demonstrates the potential of the combined in silico-in vivo approach and its clinical implications for the early treatment of patients with problematic fractures. [less ▲]

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See detailExperimentally-informed Mathematical Modelling of Oxygen Tension, Cell Viability and Proliferation in Fibrin Hydrogels
Demol, Jan; Lambrechts, Dennis; Geris, Liesbet ULg et al

in Proceedings of the TERMIS-EU meeting (2010, June)

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See detailCross-talk Modeling of Wnt, BMP and ERK Pathways during Osteochondrogenic Differentiation
Geris, Liesbet ULg; Vandeput, M.; Roberts, S. et al

in Proceedings of the TERMIS-EU meeting (2010, June)

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See detailConnecting biology and mechanics in fracture healing: an integrated mathematical modeling framework for the study of nonunions
Geris, Liesbet ULg; Vander Sloten, Jos; Van Oosterwyck, Hans

in Biomechanics & Modeling in Mechanobiology (2010), 9(6), 713-724

Both mechanical and biological factors play an important role in normal as well as impaired fracture healing. This study aims to provide a mathematical framework in which both regulatory mechanisms are ... [more ▼]

Both mechanical and biological factors play an important role in normal as well as impaired fracture healing. This study aims to provide a mathematical framework in which both regulatory mechanisms are included. Mechanics and biology are coupled by making certain parameters of a previously established bioregulatory model dependent on local mechanical stimuli. To illustrate the potential added value of such a framework, this coupled model was applied to investigate whether local mechanical stimuli influencing only the angiogenic process can explain normal healing as well as overload-induced nonunion development. Simulation results showed that mechanics acting directly on angiogenesis alone was not able to predict the formation of overload-induced onunions. However, the direct action of mechanics on both angiogenesis and osteogenesis was able to predict overload-induced nonunion formation, confirming the hypotheses of several experimental studies investigating the interconnection between angiogenesis and osteogenesis. This study shows that mathematical models can assist in testing hypothesis on the nature of the interaction between biology and mechanics. [less ▲]

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See detailThe biology of orthodontic tooth movement: a mathematical model
Van Schepdael, J.; Vander Sloten, Jos; Geris, Liesbet ULg

in Middleton, J.; Jacobs, C.; Walker, B. (Eds.) et al 9th International symposium on Computer Methods in Biomechanics and Biomedical Engineering (2010, February)

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See detailMechanical loading affects angiogenesis and osteogenesis in an in vivo bone chamber: a modeling study.
Geris, Liesbet ULg; Vandamme, Katleen; Naert, Ignace et al

in Tissue Engineering. Part A (2010), 16(11), 3353-3361

Despite a myriad of studies confirming the interaction between biology and mechanics, the exact nature of the main mechanical stimuli and their influence on the bone regeneration processes are still ... [more ▼]

Despite a myriad of studies confirming the interaction between biology and mechanics, the exact nature of the main mechanical stimuli and their influence on the bone regeneration processes are still unclear. The hypothesis of this study was that the outcome of peri-implant healing under different implant loading regimens can be explained by the influence of fluid flow on the combination of angiogenesis and osteogenesis through its influence on cell proliferation and differentiation. To investigate this hypothesis a mathematical model of bone regeneration was applied to simulate the peri-implant healing in an in vivo repeated sampling bone chamber for different axial micromechanical implant loading regimes. When mechanical loading was modeled to influence both osteogenic and angiogenic processes, a good agreement was observed between simulations and experiments concerning the amount of bone in the bone chamber, its radial and longitudinal distribution, and the bone-implant contact for different implant displacement magnitudes. [less ▲]

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See detailMathematical Modeling in Wound Healing, Bone Regeneration and Tissue Engineering
Geris, Liesbet ULg; Gerisch, Alf; Schugart, Richard C.

in Acta Biotheoretica (2010), 58(4), 355-367

The processes of wound healing and bone regeneration and problems in tissue engineering have been an active area for mathematical modeling in the last decade. Here we review a selection of recent models ... [more ▼]

The processes of wound healing and bone regeneration and problems in tissue engineering have been an active area for mathematical modeling in the last decade. Here we review a selection of recent models which aim at deriving strategies for improved healing. In wound healing, the models have particularly focused on the inflammatory response in order to improve the healing of chronic wound. For bone regeneration, the mathematical models have been applied to design optimal and new treatment strategies for normal and specific cases of impaired fracture healing. For the field of tissue engineering, we focus on mathematical models that analyze the interplay between cells and their biochemical cues within the scaffold to ensure optimal nutrient transport and maximal tissue production. Finally, we briefly comment on numerical issues arising from simulations of these mathematical models. [less ▲]

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See detailTowards a quantitative understanding of oxygen tension and cell density evolution in fibrin hydrogels
Demol, Jan; Lambrechts, Dennis; Geris, Liesbet ULg et al

in Biomaterials (2010), 32(1), 107-118

The in vitro culture of hydrogel-based constructs above a critical size is accompanied by problems of unequal cell distribution when diffusion is the primary mode of oxygen transfer. In this study, an ... [more ▼]

The in vitro culture of hydrogel-based constructs above a critical size is accompanied by problems of unequal cell distribution when diffusion is the primary mode of oxygen transfer. In this study, an experimentally informed mathematical model was developed to relate cell proliferation and death inside fibrin hydrogels to the local oxygen tension in a quantitative manner. The predictive capacity of the resulting model was tested by comparing its outcomes to the density, distribution and viability of human periosteum derived cells (hPDCs) that were cultured inside fibrin hydrogels in vitro. The model was able to reproduce important experimental findings, such as the formation of a multilayered cell sheet at the hydrogel periphery and the occurrence of a cell density gradient throughout the hydrogel. In addition, the model demonstrated that cell culture in fibrin hydrogels can lead to complete anoxia in the centre of the hydrogel for realistic values of oxygen diffusion and consumption. A sensitivity analysis also identified these two parameters, together with the proliferation parameters of the encapsulated cells, as the governing parameters for the occurrence of anoxia. In conclusion, this study indicates that mathematical models can help to better understand oxygen transport limitations and its influence on cell behaviour during the in vitro culture of cellseeded hydrogels. [less ▲]

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See detailIn vivo, in vitro, in silico: computational tools for tissue engineering
Van Oosterwyck, Hans; Truscello, S.; Demol, Jan et al

in proceedings of the 2nd International Conference on Innovation for Sustainable Production i-SUP (2010)

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See detailOptimizing The Micro-Environment In A Tissue Engineering Scaffold: A Computational Approach
Van Oosterwyck, Hans; Truscello, S.; Demol, J. et al

in proceedings of the Materialise World Conference (2010)

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See detailMathematical modelling of cell aggregates for bone tissue engineering using continuum methods
Geris, Liesbet ULg; Clarke, T.; Ashbourn, J.

in Middleton, J.; Jacobs, C.; Walker, B. (Eds.) et al 9th International symposium on Computer Methods in Biomechanics and Biomedical Engineering (2010)

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See detailExperimental and computational study of the correlation between oxygen tension, cell viability and proliferation in fibrin hydrogels
Demol, J.; Lambrechts, D.; Geris, Liesbet ULg et al

in Proceedings of the 17th conference of the European Society of Biomechanics (2010)

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See detailNumerical simulation of two-dimensional and three-dimensional axisymmetric advection-diffusion systems with complex geometries using finite-volume methods
Ashbourn, J. M. A.; Geris, Liesbet ULg; Gerisch, A. et al

in Proceedings : Mathematical, Physical & Engineering Sciences (2010), 466(2118), 1621-1643

A finite-volume method has been developed that can deal accurately with complicated, curved boundaries for both two-dimensional and three-dimensional axisymmetric advection-diffusion systems. The ... [more ▼]

A finite-volume method has been developed that can deal accurately with complicated, curved boundaries for both two-dimensional and three-dimensional axisymmetric advection-diffusion systems. The motivation behind this is threefold. Firstly, the ability to model the correct geometry of a situation yields more accurate results. Secondly, smooth geometries eliminate corner singularities in the calculation of, for example, mechanical variables and thirdly, different geometries can be tested for experimental applications. An example illustrating each of these is given: fluid carrying a dye and rotating in an annulus, bone fracture healing in mice, and using vessels of different geometry in an ultracentrifuge. [less ▲]

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See detailA cell based modelling framework for skeletal tissue engineering applications
Geris, Liesbet ULg; Van Liedekerke, Paul; Smeets, Bart et al

in Journal of Biomechanics (2010), 43(5), 887-892

In this study, a cell based lattice free modelling framework is proposed to study cell aggregate behaviour in bone tissue engineering applications. The model encompasses cell-to-cell and cell environment ... [more ▼]

In this study, a cell based lattice free modelling framework is proposed to study cell aggregate behaviour in bone tissue engineering applications. The model encompasses cell-to-cell and cell environment interactions such as adhesion, repulsion and drag forces. Oxygen, nutrients, waste products, growth factors and inhibitors are explicitly represented in the model influencing cellular behaviour. Furthermore, a model for cell metabolism is incorporated representing the basic enzymic reactions of glycolysis and the Krebs cycle. Various types of cell death such as necrosis, apoptosis and anoikis are implemented. Finally, an explicit model of the cell cycle controls the proliferation process, taking into account the presence or absence of various metabolites, sufficient space and mechanical stress. Several examples are presented demonstrating the potential of the modelling framework. The behaviour of a synchronised cell aggregate under ideal circumstances is simulated, clearly showing the different stages of the cell cycle and the resulting growth of the aggregate. Also the difference in aggregate development under ideal (normoxic) and hypoxic conditions is simulated, showing hypoxia induced necrosis mainly in the centre of the aggregate grown under hypoxic conditions. The next step in this research will be the application of this modelling framework to specific experimental set-ups for bone tissue engineering applications. (C) 2009 Elsevier Ltd. All rights reserved. [less ▲]

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See detailIn silico design of treatment strategies in wound healing and bone fracture healing
Geris, Liesbet ULg; Schugart, R.; Van Oosterwyck, H.

in Philosophical Transactions : Mathematical, Physical & Engineering Sciences (2010), 368(1920), 2683-2706

Wound and bone fracture healing are natural repair processes initiated by trauma. Over the last decade, many mathematical models have been established to investigate the healing processes in silico, in ... [more ▼]

Wound and bone fracture healing are natural repair processes initiated by trauma. Over the last decade, many mathematical models have been established to investigate the healing processes in silico, in addition to ongoing experimental work. In recent days, the focus of the mathematical models has shifted from simulation of the healing process towards simulation of the impaired healing process and the in silico design of treatment strategies. This review describes the most important causes of failure of the wound and bone fracture healing processes and the experimental models and methods used to investigate and treat these impaired healing cases. Furthermore, the mathematical models that are described address these impaired healing cases and investigate various therapeutic scenarios in silico. Examples are provided to illustrate the potential of these in silico experiments. Finally, limitations of the models and the need for and ability of these models to capture patient specificity and variability are discussed. [less ▲]

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See detailA single cell-based modelling framework for bone tissue engineering
Geris, Liesbet ULg; Van Liedekerke, Paul; Smeets, Bart et al

in Proceedings of the 17th conference of the European Society of Biomechanics (2010)

Detailed reference viewed: 15 (2 ULg)