References of "Gabriel, Annick"
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See detailAssessment of a bone biopsy technique for tiludronic acid dosage in horses: a preliminary study
Delguste, Catherine ULg; Doucet, Michèle; Gabriel, Annick ULg et al

Poster (2006, September)

Introduction Tiludronic acid as a bisphosphonate has a strong affinity for bone, making difficult the assessment of its PK profile in this deep compartment on living animals. The invasive nature of sample ... [more ▼]

Introduction Tiludronic acid as a bisphosphonate has a strong affinity for bone, making difficult the assessment of its PK profile in this deep compartment on living animals. The invasive nature of sample collection remains a limiting factor. This study was carried out in order to assess a bone biopsy technique allowing the repetition of sampling over a long period of time to dose tiludronic acid in equine bone. Material and Methods Six healthy 4- to 8-year-old Standardbred geldings were treated with tiludronic acid 1 mg/kg in a saline infusion over 30 minutes. The horses were subjected to euthanasia on days 1, 43, 57, 92, 182 and 222 post-treatment, respectively. Bone samples (test samples and larger reference samples) were taken at 4 sites per side and per horse: the lateral aspect of the metacarpal bone III (MCIII) of the forelimb, the 13th rib, the tuber coxae and the cuboïd bone. Test samples were taken with a 5-mm diameter dental drill (Implanteo™, Anthogyr), while larger reference samples were taken around the drill sample sites with an osteotome. All samples were taken immediately after euthanasia. Tiludronic acid concentrations were measured by HPLC with UV detection. Results The tuber coxae was the easiest site to sample. The sample site of the MCIII was easily accessible but due to the extreme hardness of the bone, the drill sampling was technically difficult to perform. Drill samples obtained from the 13th rib were very small, and the access more limited. Finally, the access to the cuboïd bone required considerable dissection, not performable in vivo. Extraction and dosage of tiludronic acid from the MCIII was difficult for technical reasons in most cases, in drill samples as well as in reference samples, and most (96%) of the values obtained were considered unreliable. This was also true for some samples from the 13th rib and from the cuboïd bone, to a lesser extent (42% of unreliable values for both sites). Moreover, for these two sample sites, less technical problems were encountered for the extraction and dosage of tiludronic acid in drill samples than in reference samples. No extraction or dosage problem was encountered with the tuber coxae samples. The ratio of tiludronic acid concentrations in drill versus reference samples ranged from 73% to 185% (mean: 124%), 65% to 208% (mean: 118%), and 26% to 110% (mean: 62%) respectively in the tuber coxae, 13th rib and cuboïd bone. In all but one horse, the highest concentrations in tiludronic acid were found in the tuber coxae, while the lowest values tended to be in the cuboïd bone at any time post-treatment. Tiludronic acid was still found in all bone samples 7 months after treatment. Discussion As previously reported with other tools, the drill tested in this study should permit to conveniently perform bone biopsies in the equine tuber coxae. This biopsy site would be the most appropriate for repeated sampling to dose tiludronic acid over time. This would help to design appropriate PK/PD studies with tiludronic acid in horses. This study also further confirms the long persistence of tiludronic acid in equine bone. [less ▲]

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See detailBiochemical markers of bone metabolism in Ardennes horses
Pastoret, V.; Carstanjen, V.; Hoyle, N. R. et al

Poster (2006, January)

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See detailMorphometric study of the equine fetlock and coffin joints
Riccio, Barbara; Carstanjen, Bianca; Denoix, Jean-Marie et al

in Italian Journal of Anatomy and Embryology (2006), 111

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See detailQuantitative ultrasonography for the noninvasive assessment of equine bone: a review
Carstanjen, B.; Gabriel, Annick ULg

in IPPOLOGIA (2006), 17(4), 11-15

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See detailHistological study of the horse stifle menisci in relation with ultrasonographic aspect: preliminary study
De Busscher, Virginie; Schreder, Anelaure; Busoni, Valeria ULg et al

in Italian Journal of Anatomy and Embryology (2006), 111

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See detailHistological study of equine interphalangeal joints
Toppets, Vinciane ULg; Pastoret, V.; Antoine, Nadine ULg et al

Poster (2005, July)

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See detailEvaluation of plasma carboxy-terminal cross-linking telopeptide of type I collagen concentration in horses.
Carstanjen, Bianca; Hoyle, Nicholas R; Gabriel, Annick ULg et al

in American Journal of Veterinary Research (2004), 65(1), 104-9

OBJECTIVE: To evaluate a human assay for quantification of carboxy-terminal cross-linking telopeptide of type I collagen (CTX-I), assess the influence of age on plasma CTX-I concentration, investigate the ... [more ▼]

OBJECTIVE: To evaluate a human assay for quantification of carboxy-terminal cross-linking telopeptide of type I collagen (CTX-I), assess the influence of age on plasma CTX-I concentration, investigate the relationship between plasma CTX-I and serum osteocalcin concentrations, and determine whether concentrations of plasma CTX-I or serum osteocalcin fluctuate in circadian manner in horses. HORSES: 75 clinically normal horses. PROCEDURE: Cross-reactivity between equine serum CTX-I and CTX-I antibodies in an automated electrochemiluminescent sandwich antibody assay (ECLIA) was evaluated via a specificity test (ie, dilution test) and recovery calculation. Serum osteocalcin concentration was measured with an equine-specific osteocalcin radioimmunoassay. To analyze diurnal variations in plasma CTX-I and serum osteocalcin concentrations, blood samples were obtained hourly during a 24-hour period. RESULTS: Results of the dilution test indicated good correlation (r > 0.99) between expected serum CTX-I concentrations and measured serum CTX-I concentrations. The calculated CTX-I recovery was 97.6% to 109.9%. Plasma CTX-I and serum osteocalcin concentrations were correlated. Plasma CTX-I concentration was inversely correlated with age of the horse. No significant circadian variations in plasma CTX-I and serum osteocalcin concentrations were detected. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that the fully automated CTX-I ECLIA can be used for evaluation of plasma and serum samples from horses and may be a useful tool to monitor bone metabolism changes. Horses in this study did not have notable diurnal fluctuations in serum osteocalcin and plasma CTX-I concentrations. [less ▲]

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See detailMorphology, growth and modelling of bone tissue
Toppets, Vinciane ULg; Pastoret, V.; De Behr, V. et al

in Annales de Médecine Vétérinaire (2004), 148(1), 1-13

Bone is an essential part of the squeletton. Besides its mechanic properties, bone has an important role in metabolism regulation because it acts as a reservoir for the storage of minerals essential to ... [more ▼]

Bone is an essential part of the squeletton. Besides its mechanic properties, bone has an important role in metabolism regulation because it acts as a reservoir for the storage of minerals essential to provide homeostasy. This article describes bone morphology and different ways of classification of this tissue. It gives the composition of organic and mineral extracellular bone matrix, underlines the dynamic character of bone tissue, details the cellular morphology and the metabolism of the elements acting on the synthesis/resorption mechanisms : osteoprogenitor cells, osteoblasts, osteocyts and osteoclasts. It relates the histogenesis of bone tissue and develops the different types of ossification: intramembranous, periostic, endochondral and osteonal remodeling. The last part of this article describe some dietetary and hormonal influences on bone tissue. [less ▲]

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See detailAnatomische, histologische en immunologische aspecten van de tonsille bij het schaap in het kader van de BSE problematiek
Cocquyt, G.; Gabriel, Annick ULg; Van den Broeck, W.

in Vlaams Diergeneeskundig Tijdschrift (2003), 72

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See detailAnatomical and histological aspects of the tonsils in sheep
Gabriel, Annick ULg; Cocquyt, G.; Van den Broeck, W.

in Annales de Médecine Vétérinaire (2003), 147(4, AUG-SEP), 251-258

Since the 1st April 2002, the European Union has extended the Transmissible Spongiform Encephalopathy testing in ruminants by including sheep and goats in the survey studies. In small ruminants, presence ... [more ▼]

Since the 1st April 2002, the European Union has extended the Transmissible Spongiform Encephalopathy testing in ruminants by including sheep and goats in the survey studies. In small ruminants, presence of the agent of Bovine Spongiform Encephalopathy was demonstrated in tonsils, so these are considered as specified risk materials. This article gives an overview of the anatomical localisation and histological structure of the tonsils in small ruminants. Anatomically, 6 different tonsils can be distinguished: 3 are located in the oropharyngeal tract (tonsilla palatina, tonsilla lingualis, tonsilla veli palatini), 2 in the nasopharyngeal tract (tonsilla pharyngea and tonsilla tubaria), and one in the laryngopharyngeal tract (tonsilla paraepiglottica). Several tonsils show a cryptic overlying epithelium (cryptic tonsil) whereas other don't (non-cryptic tonsil). Several immunological features of the tonsils are described. [less ▲]

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See detailPhysiology of reproduction and endocrinology in cervids. A review
Drion, Pierre ULg; Hanzen, Christian ULg; Wirth, Delphine - in memoriam et al

in Annales de Médecine Vétérinaire (2003), 147(5), 291-313

Knowledge about cervids are rapidly growing. This work aims to present the more recent scientific information on reproductive physiology and endocrinology of the cervids: anatomy of the genital tract ... [more ▼]

Knowledge about cervids are rapidly growing. This work aims to present the more recent scientific information on reproductive physiology and endocrinology of the cervids: anatomy of the genital tract, length of breeding season, parameters and endocrinology of the reproductive cycle, endocrinology of pregnancy. It also aims to highlight differences between cervids and domestic ruminants such as embryonic diapause observed in Capreolus capreolus. [less ▲]

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See detailThe course of some bone remodelling plasma metabolites in healthy horses and in horses offered a calcium-deficient diet.
de Behr, V.; Daron, D.; Gabriel, Annick ULg et al

in Journal of Animal Physiology & Animal Nutrition (2003), 87(3-4), 149-59

An inquiry was carried out to assess the concentrations of plasma metabolites related to bone remodelling in 21 saddle horses of Warmblood breed aged 4-26 years, five draught horses of Ardennes breed aged ... [more ▼]

An inquiry was carried out to assess the concentrations of plasma metabolites related to bone remodelling in 21 saddle horses of Warmblood breed aged 4-26 years, five draught horses of Ardennes breed aged 4-10 years, and 10 Ardennes foals aged 9-11 months. They were fed according to normal feeding practice in Belgium. The changes in some bone remodelling plasma metabolite concentrations were studied when an unbalanced diet was offered and later corrected for four Warmblood horses. Bone formation was evaluated by bone alkaline phosphatase (BALP), total alkaline phosphatase (TALP) and osteocalcin (bone gla-protein, OC). Bone resorption was assessed by hydroxyproline (HYP). Total calcium, ionized calcium, phosphorus (P) and 25-hydroxyvitamin D3 [25-(OH)D] concentrations were more or less constant. The comparison of four bone remodelling factors between the Ardennes and Warmblood horses showed higher concentrations in the Ardennes breed. Bone marker concentrations decreased according to age. The correction of the unbalanced Ca : P diet induced inconsistent effects at plasma level. The interpretation of the different bone parameters appeared to be difficult if not associated with other parameters such as a complete anamnesis and clinical examination of the animal in addition to dietary evaluation. [less ▲]

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See detailPrélèvement de l’isthme encéphalique pour la recherche de l’encéphalopathie spongiforme transmissible chez le mouton
Gabriel, Annick ULg; Jacobs, Christine ULg; Simoens, P.

in Annales de Médecine Vétérinaire (2002), 146(5), 295-300

Depuis le premier avril 2002, une directive de l'Union Européenne contraint la Belgique à tester les chèvres et les moutons, en plus des bovins, dans le cadre de la recherche d'encéphalopathie spongiforme ... [more ▼]

Depuis le premier avril 2002, une directive de l'Union Européenne contraint la Belgique à tester les chèvres et les moutons, en plus des bovins, dans le cadre de la recherche d'encéphalopathie spongiforme transmissible. Comme chez les bovins, le diagnostic est réalisé à partir d'un prélèvement de l'isthme encéphalique au niveau de l'obex. Des différences morphologiques entre l'isthme encéphalique des moutons et chèvres et celui des bovins ont nécessité quelques ajustements de la méthode de prélèvement. Dans cette étude, les particularités techniques du prélèvement et certaines caractéristiques morphologiques du cerveau ovin sont décrites. Les différences majeures, par rapport aux bovins, sont d'une part les dimensions plus petites, et d'autre part le rétrécissement caudal très marqué de l'isthme encéphalique. [less ▲]

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See detailStaalname van de hersenstam voor TSE-Onderzoek bij het schaap
Jacobs, C.; Gabriel, Annick ULg; Simoens, P.

in Vlaams Diergeneeskundig Tijdschrift (2002), 71

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See detailIngestion and metabolic profile in horses offered lucerne or chalk as a source of calcium
de Behr, V.; Daron, D.; Gabriel, Annick ULg et al

in Proceedings of the British Society of Animal Science (2002)

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See detailLooking for human therapeutic intervention in the healing of fractures
Udrescu, M.; Gabriel, Annick ULg

Poster (2002)

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See detailLatency and Reactivation of a Glycoprotein E Negative Bovine Herpesvirus Type 1 Vaccine: Influence of Virus Load and Effect of Specific Maternal Antibodies
Lemaire, Mylène; Schynts, Frédéric; Meyer, Gilles et al

in Vaccine (2001), 19(32), 4795-804

The effects of the vaccination of neonatal calves with a glycoprotein E (gE)-negative bovine herpesvirus type 1 (BHV-1) were investigated in naive and passively immunised calves either with the ... [more ▼]

The effects of the vaccination of neonatal calves with a glycoprotein E (gE)-negative bovine herpesvirus type 1 (BHV-1) were investigated in naive and passively immunised calves either with the recommended dose or a 5-fold concentrated one. After inoculation (PI), all calves excreted the virus vaccine except three passively immunised calves inoculated with the lower titre. No antibody response could be detected in passively immunised calves, whatever the dose used, and they all became BHV-1 seronegative and remained so after dexamethasone treatment (PDT). Nevertheless, as shown by a gamma-interferon assay, all calves that excreted the vaccine PI developed a cell-mediated immune response and a booster response was observed PDT, suggesting viral reactivation. The vaccine virus was recovered PDT from nasal secretions in two calves and BHV-1 DNA were detected in trigeminal ganglia from five calves belonging to all inoculated groups. The results show that the BHV-1 gE-negative vaccine can establish latency not only in naive but also in passively immunised neonatal calves after a single intranasal inoculation. Moreover, this study shows for the first time that the gE-negative vaccine, when used in passively immunised calves, can lead to seronegative vaccine virus carriers. [less ▲]

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