Metabolomic analysis of Echinacea sp. by H-1 Nuclear magnetic resonance spectrometry and multivariate analysis techniques

in Planta Medica (2008), 74(9), 1089-1089

In vitro antiplasmodial activity of five plants used in Benin in traditional medicine to treat malaria

in Planta Medica (2008), 74(9), 1002-1002

Antiplasmodial and antitrypanosomal activity of Triclisia sacleuxii (Pierre) Diels

in Phytomedicine : International Journal of Phytotherapy and Phytopharmacology (2008), 15(9), 728-733

The antiplasmodial and antitrypanosomal activities of Triclisia sacleuxii (Pierre) Diels were investigated on three Plasmodium falciparum strains [FcB1, 3D7 (chloroquine – sensitive) and W2 (chloroquine – ... [more ▼]

The antiplasmodial and antitrypanosomal activities of Triclisia sacleuxii (Pierre) Diels were investigated on three Plasmodium falciparum strains [FcB1, 3D7 (chloroquine – sensitive) and W2 (chloroquine – resistant) strains] and on Trypanosoma brucei Tbsf 221. Roots, stems and leaves ethanolic extracts as well as crude tertiary and quaternary alkaloids fractions were considered. Whereas the ethanolic extracts and quaternary crude alkaloids fractions exhibited no significant activity, the roots and stems tertiary alkaloid fractions revealed interesting growth inhibition against the Plasmodium FcB1 and Trypanosoma Tbsf 221 strains. The IC50 were 1.04 and 0.89 g/ml (roots), 2.50 and 0.91 g/ml (stems), respectively. The leaves tertiary alkaloids fraction also showed a promising antitrypanosomal activity (IC50 : 1.85 g/ml). Phytochemical analysis of the roots tertiary alkaloids fraction yielded four major compounds, phaeanthine, N-methylapateline, 1,2-dehydroapateline and 1,2-dehydrotelobine, which were identified on the basis of their spectroscopic data. The four compounds displayed (in vitro) antitrypanosomal activity with IC50 of 2.68, 1.19, 1.06 and 1.11 µM, respectively. They also demonstrated antiplasmodial activity on Plasmodium falciparum 3D7, with IC50 of 1.72, 0.93, 1.39 and 12.4 µM respectively and on the chloroquine – resistant W2 with IC50 of 0.35, 1.10, 1.63, 1.52 µM. [less ▲]

A la recherche de substances naturelles antiplasmodiales et cytotoxiques

Scientific conference (2007, December 10)

In vitro cytotoxicity of some medicinal plants from Georgian amaryllidaceae

in Phytotherapy Research (2007), 21(7), 622-624

Using an ethnomedical data approach, some Georgian plants, which are used in Georgian traditional medicine for cancer or non-cancer diseases, were collected and evaluated for cytotoxic activity. The ... [more ▼]

Using an ethnomedical data approach, some Georgian plants, which are used in Georgian traditional medicine for cancer or non-cancer diseases, were collected and evaluated for cytotoxic activity. The cytotoxic effect of the methanol extracts of species from the genera Galanthus and Leucojum was evaluated in vitro on three human cell lines (Hela, ephitheloid cervix carcinoma; HCT-116, colon carcinoma; HL-60, acute myeloid leukaemia). Cell type cytotoxic specificity was observed for some extracts. Overall, the HCT-116 cells were much more sensitive to most of the extracts than were the other cell lines. Plants that showed pronounced cytotoxic activity will be further evaluated for the possible isolation of active antitumour compounds. Copyright (C) 2007 John Wiley [less ▲]

In vivo antimalarial activity of isosungucine, an indolomonoterpenic alkaloid from Strychnos icaja

in Planta Medica (2007), 73(5), 478-479

Isosungucine (1) is a quasi-symmetric bisindolomonoterpenoid alkaloid isolated from the roots of Strychnos icaja. The in vivo antimalarial activity against the P. vinckei petteri murine strain was ... [more ▼]

Isosungucine (1) is a quasi-symmetric bisindolomonoterpenoid alkaloid isolated from the roots of Strychnos icaja. The in vivo antimalarial activity against the P. vinckei petteri murine strain was determined. In the Peters 4-day suppressive test, 1 suppressed the parasitemia by almost 50 percent on day 4 at the dose of 30 mg/kg by intraperitoneal route. [less ▲]

Discovery of a natural thiamine adenine nucleotide

in Nature Chemical Biology (2007), 3(4), 211-212

Several important cofactors are adenine nucleotides with a vitamin as the catalytic moiety. Here, we report the discovery of the first adenine nucleotide containing vitamin B1: adenosine thiamine ... [more ▼]

Several important cofactors are adenine nucleotides with a vitamin as the catalytic moiety. Here, we report the discovery of the first adenine nucleotide containing vitamin B1: adenosine thiamine triphosphate (AThTP, 1), or thiaminylated ATP. We discovered AThTP in Escherichia coli and found that it accumulates specifically in response to carbon starvation, thereby acting as a signal rather than a cofactor. We detected smaller amounts in yeast and in plant and animal tissues. [less ▲]

Nematocidal compounds from the seeds of Balanites aegyptiaca isolation and structure elucidation.

in International Journal of Pharmacology (2007), 3(3), 280-284

A study was conducted to characterize the anthelmintic activity and to isolate the main nematocidal agent of B. aegyptiaca plant. The anthelmintic activity was evaluated in vitro by means of an original ... [more ▼]

A study was conducted to characterize the anthelmintic activity and to isolate the main nematocidal agent of B. aegyptiaca plant. The anthelmintic activity was evaluated in vitro by means of an original anthelmintic assay using Caenorhabditis elegans as a biological model. Fluorescence microscopy was used for the determination of the percentage of worm death. The structure elucidation was based on NMR, mass spectroscopic analysis and chemical methods. A bioassay-directed fractionation of the aqueous extract of B. aegyptiaca led to the isolation of balanitin-7 (also named diosgenin 3beta-O-beta-D-xylopyranosyl-(1->3)-beta-D-glucopyranosyl-(1->4)-[alpha- L-rhamnopyranosyl-(1->2)]-beta-D-glucopyranoside), as being the principal nematicidal agent. These data indicate that balanitin-7 has an appreciable nematocidal activity, which is not mediated by inducing an anti-acetylcholinesterase activity. [less ▲]

In vitro metabolisation study of several 3-alkylamino-4H-1,2,4-benzothiadiazine 1,1-dioxides as ATP-sensitive potassium channel openers : combined use of LC-Q-TOF MS/MS and LC-SPE-NMR

Poster (2006, November 18)

Combined use of LC-SPE-NMR and LC-MS for the metabolites characterization of ATP-sensitive potassium channel openers belonging to 3-alkylamino-4H-1,2,4-arylthiadiazine 1,1-dioxides

Poster (2006, August)