References of "Francis, Frédéric"
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See detailLa membrane du globule gras du lait (MFGM) : une composition et une structure originale.
Bodson, Pascal; Vanderghem, Caroline ULg; Danthine, Sabine ULg et al

Poster (2008, January 23)

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See detailCharacterization of a novel aphid prenyltransferase displaying dual geranyl/farnesyl diphosphate synthase activity
Vandermoten, Sophie ULg; Charloteaux, Benoît ULg; Santini, S. et al

in FEBS Letters (2008), 582(16), 19281934

We report on the cDNA cloning and characterization of a novel short-chain isoprenyl diphosphate synthase from the aphid Myzus persicae. Of the three IPPS cDNAs we cloned, two yielded prenyltransferase ... [more ▼]

We report on the cDNA cloning and characterization of a novel short-chain isoprenyl diphosphate synthase from the aphid Myzus persicae. Of the three IPPS cDNAs we cloned, two yielded prenyltransferase activity following expression in Escherichia coli; these cDNAs encode identical proteins except for the presence, in one of them, of an N-terminal mitochondrial targeting peptide. Although the aphid enzyme was predicted to be a farnesyl diphosphate synthase by BLASTP analysis, rMpIPPS, when isopentenyl diphosphate and dimethylallyl diphosphate are supplied as substrates, typically generated geranyl diphosphate (C10) as its main product, along with significant quantities of farnesyl diphosphate (C15). Analysis of an MpIPPS homology model pointed to substitutions that could confer GPP/FPP synthase activity to the aphid enzyme. [less ▲]

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See detailVolatile blends and oxylipins profiles in potato leaves (Solanum tuberosum L.) exposed to insects infestations and mechanical wounding.
Gosset, V.; Göbel, Cornelia; Harmel, N. et al

Conference (2008)

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See detailBreeding Sites Of Bluetongue Vectors In Northern Europe
Zimmer, Jean-Yves ULg; Haubruge, Eric ULg; Francis, Frédéric ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2008), 162(4), 131

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See detailDistribution Of Potential Bluetongue Vectors On Belgium Farms
Zimmer, Jean-Yves ULg; Haubruge, Eric ULg; Francis, Frédéric ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2008), 162(21), 700

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See detailProteome analysis of the bovine milk fat globule: Enhancement of membrane purification
Vanderghem, Caroline ULg; Blecker, Christophe ULg; Danthine, Sabine ULg et al

in International Dairy Journal (2008), 18(9), 885-893

A simple and rapid procedure was cl developed for the extraction of the milk fat globule membrane from milk removes the large majority of the skim milk proteins for proteome analysis. In order to improve ... [more ▼]

A simple and rapid procedure was cl developed for the extraction of the milk fat globule membrane from milk removes the large majority of the skim milk proteins for proteome analysis. In order to improve the extraction and the solubilization of the hydrophobic membrane proteins for subsequent two-dimensional gel electrophoresis, four detergents (3-[(3-cholamidopropyl)dimethylammoniol-1-propanesulfonate, amidosulfobetaine-14, sodium lauroyl sarcosinate and sodium deoxycholate) were tested in the sample preparation, associated with a sonication step. Zwitterionic detergents were shown to be efficient in recovering integral and peripheral proteins from membrane material. Spots were identified by matrix-assisted laser desorption/ionization tandem-time-of-flight (MALDI-TOF/TOF). The advantages of MALDI-TOF/TOF (speed, easiness of analysis, good sensitivity and high mass accuracy) were demonstrated on the milk fat globule membrane proteome investigation. Identified proteins are implicated in a wide range of functions including fat secretion and transport, protein trafficking and regulation. (C) 2008 Elsevier Ltd. All rights reserved. [less ▲]

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See detailLa biosynthèse des isoprenoides chez les pucerons: une cible potentielle de nouveaux bio-insecticides?
Vandermoten, Sophie ULg; Cusson, Michel; Francis, Frédéric ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2008), 12(4),

Actuellement, le contrôle des pucerons repose en grande partie sur l'utilisation d'insecticides de synthèse. Toutefois, l'effet néfaste de ceux-ci sur l'environnement, ainsi que le développement croissant ... [more ▼]

Actuellement, le contrôle des pucerons repose en grande partie sur l'utilisation d'insecticides de synthèse. Toutefois, l'effet néfaste de ceux-ci sur l'environnement, ainsi que le développement croissant de populations résistantes conduit à rechercher de nouvelles méthodes de lutte. Parmi celles-ci, une des voies actuellement envisagée est la formulation de nouveaux bio-insecticides ciblant et perturbant les fonctions biochimiques de l'insecte. En raison de la haute spécificité des agents utilisés, ces méthodes de contrôle des ravageurs ne présentent généralement que peu de risque pour l'environnement, les espèces non ciblées et la santé humaine. Dans le contexte de la lutte contre les pucerons, la voie de biosynthèse des isoprénoïdes, et plus particulièrement les enzymes de la famille des " short-chain " isoprényle diphosphate synthases, s'avère être une cible intéressante pour le développement de ces nouveaux agents de contrôle. En effet, chez les pucerons, cette voie de biosynthèse est associée à la production des mono- et sesquiterpènes, deux classes d'isoprénoïdes jouant des rôles importants au niveau de la physiologie des pucerons [less ▲]

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See detailEmission of alarm pheromone by non-preyed aphid colonies
Almohamad, Raki; Verheggen, François ULg; Francis, Frédéric ULg et al

in Journal of Applied Entomology (2008), 132(8), 601-604

The sesquiterpene (E)-beta-farnesene (E beta f) is the primary component of the alarm pheromone of most aphid species. It is released in response to physical stress including attack by natural enemies and ... [more ▼]

The sesquiterpene (E)-beta-farnesene (E beta f) is the primary component of the alarm pheromone of most aphid species. It is released in response to physical stress including attack by natural enemies and causes aphids to cease feeding and disperse. E beta f also acts as a kairomonal cue for aphid natural enemies. In this study, we collected the headspace volatiles released by aphid colonies of different sizes. Gas chromatography-mass spectrometry analysis demonstrated the presence of E beta f in the absence of predator attack. A quadratic relationship was found between the released (E)-beta-farnesene amounts and aphid colony size. Behavioural impact of aphid alarm pheromone towards Episyrphus balteatus female oviposition behaviour was also demonstrated in this work. These results highlight the primary role of the small but continuous release of aphid alarm pheromone in mechanisms of decision-making by aphid predators during their foraging and egg-laying behaviour. [less ▲]

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See detailInvestigation of carbohydrate binding property of a fungal lectin from Xerocomus chrysenteron and potential use on Myzus persicae aphid.
Jaber, Karimi; Cuartero Diaz, Gaetan; Haubruge, Eric ULg et al

in Communications in Agricultural and Applied Biological Sciences (2008), 73(3), 629-38

In recent years encoding insecticidal Lectins have been suggested as one of the promising methods against insect pests and have been engineered successfully into a variety of crops including wheat, rice ... [more ▼]

In recent years encoding insecticidal Lectins have been suggested as one of the promising methods against insect pests and have been engineered successfully into a variety of crops including wheat, rice, tobacco and potatoes. Xerocomus chrysenteron Lectin (XCL) has a high hemagglutinating activity and results obtained from sugar specificity assay showed to have specific affinity to Galactose and N-Acetylgalactosamine (GalNAc). In previous studies, XCL was shown to have negative effects on some insect pests, including aphids. In the present study, the effects of different carbohydrates including D-glucose, D-mannose, D-galactose and GalNAc, associated with 0.1% XCL (w/v) in artificial diet was investigated to assess the evolution of the lectin toxicity toward Myzus persicae aphid during 7 days. M. persicae, a polyphagous aphid, showed no significant differences of mortality when fed with the XCL lectin associated with Glucose and Mannose or fed on XCL diet only. At the opposite, the mortality rates related to artificial diet supplemented with Galactose or GalNAc and XCL were significantly reduced. There was then a significant mortality difference between M. persicae fed on an artificial diet incorporated specific carbohydrate binding Lectin with those fed with lectin only. The potential use of this particular fungal Lectin (XCL) with more specific carbohydrate binding will be discussed in relation to the development of bio-insecticide and integrated pest management. [less ▲]

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See detailDiscrimination of parasitized aphids by a hoverfly predator: effects on larval performance, foraging, and oviposition behavior
Almohamad, Raki; Verheggen, François ULg; Francis, Frédéric ULg et al

in Entomologia Experimentalis et Applicata (2008), 128(1), 73-80

The choice of oviposition site by female aphidophagous predators is crucial for offspring performance, especially in hoverflies whose newly hatched larvae are unable to move over large distance. Predator ... [more ▼]

The choice of oviposition site by female aphidophagous predators is crucial for offspring performance, especially in hoverflies whose newly hatched larvae are unable to move over large distance. Predator and parasitoid interactions within the aphidophagous guild are likely to be very important in influencing the choices made by predatory hoverfly females. In the present study, the foraging and oviposition behavior of the aphidophagous hoverfly Episyrphus balteatus DeGeer (Diptera: Syrphidae) was investigated with respect to the parasitized state of its aphid prey, Acyrthosiphon pisum Harris (Homoptera: Aphididae), that were parasitized by Aphidius ervi Haliday (Hymenoptera: Aphidiidae). We also recorded the number of eggs laid by hoverfly females when subjected to parasitized aphids. Furthermore, we studied the influence of being fed with parasitized aphids on hoverfly larval performance. Hoverfly females did not exhibit any preference for plants infested with unparasitized or aphids parasitized for 7 days. On the other hand, plants infested with mummies or exuvia were less attractive for E. balteatus. These results were correlated with (i) the number of eggs laid by E. balteatus females and (ii) larval performance. Thus, our results demonstrate that E. balteatus behavior is affected by parasitoid presence through their exploitation of aphid colonies. Indeed, hoverfly predators select their prey according to the developmental state of the parasitoid larvae. [less ▲]

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See detailIdentification of aphid salivary proteins: a proteomic investigation of Myzus persicae.
Harmel, Nicolas ULg; Letocart, E.; Cherqui, A. et al

in Insect Molecular Biology (2008), 17(2), 165-74

The role of insect saliva in the first contact between an insect and a plant is crucial during feeding. Some elicitors, particularly in insect regurgitants, have been identified as inducing plant defence ... [more ▼]

The role of insect saliva in the first contact between an insect and a plant is crucial during feeding. Some elicitors, particularly in insect regurgitants, have been identified as inducing plant defence reactions. Here, we focused on the salivary proteome of the green peach aphid, Myzus persicae. Proteins were either directly in-solution digested or were separated by 2D SDS-PAGE before trypsin digestion. Resulting peptides were then identified by mass spectrometry coupled with database investigations. A homemade database was constituted of expressed sequence tags from the pea aphid Acyrtosiphon pisum and M. persicae. The databases were used to identify proteins related to M. persicae with a nonsequenced genome. This procedure enabled us to discover glucose oxidase, glucose dehydrogenase, NADH dehydrogenase, alpha-glucosidase and alpha-amylase in M. persicae saliva. The presence of these enzymes is discussed in terms of plant-aphid interactions. [less ▲]

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See detailAphid – ant mutualism : How do aphids focus ant foraging ?
Verheggen, François ULg; Diez, Lise; Detrain, Claire et al

Poster (2008)

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See detailRole of prey–host plant associations on Harmonia axyridis and Episyrphus balteatus field distribution and efficiency.
Alhmedi, Ammar; Haubruge, Eric ULg; Francis, Frédéric ULg

in Entomologia Experimentalis et Applicata (2008), 128

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