References of "Focant, Jean-François"
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See detailFatty Matrices Cleaned-up with a Di erent Approach
Calaprice, Chiara ULg; Focant, Jean-François ULg

Scientific conference (2015, October)

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See detailHigh Resolution Gas Chromatography coupled to Mass Spectrometry for the analysis of volatile organic compounds
Savareear, Benjamin ULg; Brokl, M; Wright, C et al

Scientific conference (2015, September)

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See detailCadaveric VOC profiling from human internal cavity
Stefanuto, Pierre-Hugues ULg; Varlet, Vincent; Grabherr, Silke et al

Conference (2015, September)

The processes tacking place during body decomposition is not yet totally understood. Soon after death, the different parts of a body start to decompose. This phenomenon conduct to the production of gases ... [more ▼]

The processes tacking place during body decomposition is not yet totally understood. Soon after death, the different parts of a body start to decompose. This phenomenon conduct to the production of gases inside or around the different organs. These gases reservoirs can be located using imaging tools as post-mortem computed tomography (PMCT) and subsequently sample (1). The analysis of very volatile compounds inside these internal cavities already provides useful information for legal medicine investigations (2). It gives valuable information about the potential cause of death avoiding the invasiveness of the autopsy procedure. This project wanted to perform an untargeted screening of this internal cavity in order to monitor the decomposition process taking place inside a cadaver. The analytical method was base on previously used techniques for complete decomposition analysis (3-4). This study wanted to investigate the volatile organic compounds (VOC) mixture present in these cavities. To achieve this task, we applied solid phase micro-extraction (SPME) combined with two-dimensional gas chromatography coupled to high-resolution time of flight mass spectrometer (GC×GC-HRTOFMS). The first point was to demonstrate the ability of GC×GC-HRTOFMS to detect these VOC. The combination of different statistical approaches conducted to establishment of potential biomarkers list. Base on these biomarkers identification, different decomposition stages were observed for different organs in the same body. The cardiac area seems to decompose faster than the other tissues studied in this project. References 1. Levy A.D. et al., Am. J. Forensic Med. Pathol. (2010) 31, 12-17. 2. Varlet V. et al., Anal. Chim. Acta (2013) 784, 42-46. 3. Stadler S. et al., Anal Chem (2013) 85, 998–1005. 4. Stefanuto et al. CPC (2014) 79, 786-789. [less ▲]

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See detailInvestigation of volatile mixture emit by human tissues degradation
Stefanuto, Pierre-Hugues ULg; Dubois, Lena; Lloyd, Rebecca et al

Conference (2015, September)

Human rescue dog unit are the more efficient tool to locate cadaver or people trap after a natural disaster. However, the dog training process is long and expensive. Dog trainers are always looking for a ... [more ▼]

Human rescue dog unit are the more efficient tool to locate cadaver or people trap after a natural disaster. However, the dog training process is long and expensive. Dog trainers are always looking for a better understanding of the dog olfaction matching to improve their training methods (1). During these training sessions, dogs are usually trained to locate body pieces due to the difficulty to obtain a full cadaver. Moreover, some training aid solution are available but their compositions and their efficiencies are not worldwide recognized (2). To answer these questions, this project is monitoring the headspace human organs during the decomposition process. Five different organs are used: heart, lung, liver, kidney and blood. Every organs are sampled in triplicates and let to decompose in glass jar. Regularly, the headspace of the jar is sample by dynamic pumping to sorbent tubes that will further be injected on comprehensive two-dimensional gas chromatography system (GC×GC). GC×GC is a powerful analytical tool that allows the complete resolution of “the smell of death”, i.e. the decomposition odor (3-4). The compounds identified in the organs headspace can be compared with the one coming from decomposition studies on full bodies to establish a biomarkers list that will further be used for dog training solution elaboration. References 1. Hoffman et al. FSI (2009) 186, 6-13. 2. Stadler et al. J. Chrom. A. 1255, 202-206. 3. Stefanuto et al. CPC (2014) 79, 786-789. 4. Perrault et al. J. Sep. Sci. (2015) 38, 73-80. [less ▲]

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See detailExploring the volatome of different cancer cell lines
Stefanuto, Pierre-Hugues ULg; Pesesse, Romain ULg; Bertrand, Virginie ULg et al

in European Respiratory Journal (2015), 46(suppl 59), 3975

As reported by the American Cancer Society, lung cancers have the highest death rate, compared to other types of cancer. The main reason is the lack of early stage non-invasive screening methods. Indeed ... [more ▼]

As reported by the American Cancer Society, lung cancers have the highest death rate, compared to other types of cancer. The main reason is the lack of early stage non-invasive screening methods. Indeed, the death rate of the other most abundant cancers is decreasing due to the implementation of large-scale population screening techniques. It is believed that the death rate of lung cancer could be lowered by developing and implementing more efficient diagnostic methods1. A large number of diseases appear to have a volatile signature present in exhaled air. In practice, analytical breath profiling offers solutions for early detection of different kind of lung infections2. For example, cystic fibrosis patients show a different volatile organic compound (VOC) profile because of the bacterial colonization that localizes in their lungs. A similar behavior is observed for patient suffering from lung cancer. However, the list of recognized volatile biomarkers of lung cancer is still scarce and could be improved. In spite of this, the biological variability of exhaled air profile makes the biomarkers identification challenging. In the hope of contributing to a better understanding, we used comprehensive two-dimensional gas chromatography coupled to time of light mass spectrometry (GC×GC-TOFMS) to investigate the headspace of cancer cells. A procedure has been developed to sample cell culture headspace. After proper data treatment, univariate and multivariate statistics were compared for isolation of potential cancer biomarkers. Further tentative identification was also carried out by using high-accuracy MS analyzers as detection techniques. 1 Siegel, R. et al. Cancer statistics 2013 2 Di Francesco, F. et al. Microchemical Journal 2005 . [less ▲]

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See detailDetection of decomposition volatile organic compounds in soil following removal of remains from a surface deposition site
Perrault, Katelynn A.; Stefanuto, Pierre-Hugues ULg; Stuart, Barbara H. et al

in Forensic Science, Medicine & Pathology (2015), 11(3), 376-387

Purpose Cadaver-detection dogs use volatile organic compounds (VOCs) to search for human remains including those deposited on or beneath soil. Soil can act as a sink for VOCs, causing loading of ... [more ▼]

Purpose Cadaver-detection dogs use volatile organic compounds (VOCs) to search for human remains including those deposited on or beneath soil. Soil can act as a sink for VOCs, causing loading of decomposition VOCs in the soil following soft tissue decomposition. The objective of this study was to chemically profile decomposition VOCs from surface decomposition sites after remains were removed from their primary location. Methods Pig carcasses were used as human analogues and were deposited on a soil surface to decompose for 3 months. The remains were then removed from each site and VOCs were collected from the soil for 7 months & Katelynn A. Perrault katelynn.perrault@uts.edu.au thereafter and analyzed by comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry (GC9GC–TOFMS). Results Decomposition VOCs diminished within 6 weeks and hydrocarbons were the most persistent compound class. Decomposition VOCs could still be detected in the soil after 7 months using Principal Component Analysis. Conclusions This study demonstrated that the decompo- sition VOC profile, while detectable by GC9GC–TOFMS in the soil, was considerably reduced and altered in com- position upon removal of remains. Chemical reference data is provided by this study for future investigations of canine alert behavior in scenarios involving scattered or scav- enged remains. [less ▲]

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See detailBreaking the Code of Cadaveric Decomposition
Stefanuto, Pierre-Hugues ULg; Focant, Jean-François ULg

Scientific conference (2015, June)

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See detailUltra-trace measurement of dechloranes in food and feed
Calaprice, Chiara ULg; L'Homme, Benjamin; Calvano, Cosima Damiana et al

Scientific conference (2015, June)

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See detailInvestigation of Mainstream Tobacco Smoke Particulate Phase by HS-SPME-GC×GC-HRTOFMS
Lizak, Radoslaw ULg; Huston-Fulmer, L; Binkley, J et al

Poster (2015, May)

Detailed reference viewed: 5 (1 ULg)
See detailAre Dechloranes a source of concern? Food control and dietary intake estimation
Calaprice, Chiara ULg; L'Homme, B; Calvano, DC et al

Scientific conference (2015, May)

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See detailTrappist Beers Analysis, Exploring the Secrets of Monks
Focant, Jean-François ULg; L'Homme, B; Allen, C et al

Conference (2015, May)

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See detailInvestigation of Mainstream Tobacco Smoke Particulate Phase by HS-SPME-GC×GC-HRTOFMS
Lizak, Radoslaw ULg; Brokl, M; Wright, C et al

Scientific conference (2015, May)

Detailed reference viewed: 7 (2 ULg)