References of "Fillet, Marianne"
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See detailSeparation, identification and quantitation of ceramides in human cancer cells by liquid chromatography–electrospray ionization tandem mass spectrometry
Fillet, Marianne ULg; Van Heugen, Jean-Claude ULg; Servais, Anne-Catherine ULg et al

in Journal of Chromatography. A (2002), 949

Ceramides are important intracellular second messengers that play a role in the regulation of cell growth, differentiation and programmed cell death. Qualitative and quantitative analysis of these second ... [more ▼]

Ceramides are important intracellular second messengers that play a role in the regulation of cell growth, differentiation and programmed cell death. Qualitative and quantitative analysis of these second messengers requires sensitive and specific analytical methods to detect endogenous levels of individual ceramide species and to differentiate between them. Nine synthetic ceramides were separated by liquid chromatography coupled to tandem mass spectrometry on a C bonded silica 18 column. The lipids were eluted in gradient elution mode using a mixture of water, acetonitrile and 2-propanol as mobile phase. They were detected by reaction monitoring performed on positive ion electrospray generated ions. Collision-induced fragmentations conducted on ceramides produced a well characteristic product ion at m/z 264, making multiple reaction monitoring (MRM) well suited for various ceramides quantitative measurements. After optimization of the extraction step, the proposed methodology was able to identify and quantify different ceramide species issued from human cancer cells. The method could be validated for C , C and C ceramides, quantified at the nanogram level. The validation exhibits good 16 18 20 results with respect to linearity, accuracy and precision. [less ▲]

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See detailEnantiomeric Separations of Drugs Using Mixtures of Charged and Neutral Cyclodextrins
Fillet, Marianne ULg; Hubert, Philippe ULg; Crommen, Jacques ULg

in Journal of Chromatography. A (2000), 875(1-2), 123-34

An overview on the use of mixtures of neutral and charged cyclodextrins as chiral additives for the enantioseparation of drugs by capillary electrophoresis is presented. These so called dual cyclodextrin ... [more ▼]

An overview on the use of mixtures of neutral and charged cyclodextrins as chiral additives for the enantioseparation of drugs by capillary electrophoresis is presented. These so called dual cyclodextrin systems can often provide unique selectivities. A brief theoretical background illustrating the influence of the chiral discrimination ability and the effective mobility of the two cyclodextrins on the overall selectivity of the enantiomeric separation is given. Typical examples of applications in the pharmaceutical field, based on the simultaneous use of a charged (cationic or anionic) and neutral cyclodextrins, are described. [less ▲]

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