References of "Fauconnier, Marie-Laure"
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See detailIdentification de la composition en arômes des miels de la région orientale du Maroc (Communication orale)
Abeslami, Azzedine; Fauconnier, Marie-Laure ULg; Sindic, Marianne ULg et al

Scientific conference (2014, May 15)

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See detailLes huiles essentielles
Rotschild, Aude; Benini, Céline; Fauconnier, Marie-Laure ULg

Scientific conference (2014, May 14)

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See detailSubstitution du houblonnage à cru par l’adjonction d’une liqueur naturelle
Deckers, Sylvie; Fauconnier, Marie-Laure ULg

Report (2014)

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Peer Reviewed
See detailAllo- and autoinhibition in barley and great brome: a laboratory study
Bouhaouel, Imen ULg; Gfeller, Aurélie; Fauconnier, Marie-Laure ULg et al

Conference (2014, February 08)

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See detailImpact of abiotic stresses on volatile organic compound production of field crops and grasslands
Digrado, Anthony ULg; Mozaffar, Ahsan ULg; Bachy, Aurélie ULg et al

Poster (2014, February 07)

Abiotic and biotic stresses are known to alter biogenic volatile organic compound (BVOC) emission from plants. With the climate and global change, BVOC emissions are likely to increase. This increase on ... [more ▼]

Abiotic and biotic stresses are known to alter biogenic volatile organic compound (BVOC) emission from plants. With the climate and global change, BVOC emissions are likely to increase. This increase on BVOC emissions could be driven by many environmental parameters like temperature, ozone and light availability for photosynthesis although it is still difficult to predict the impact of some environmental parameters, environmental controls on BVOC emission being species and BVOC-dependent. These BVOC are involved in a wide range of interactions of plants with their environment and these interactions could be affected by the global change. Moreover, BVOC also play a key role in the atmospheric chemistry and may contribute to ozone formation and an increase in methane lifetime, strengthening the global change. Yet, due to technical limitation, there are few studies examining the impact of multiple co-occurring stresses on BVOC emission at the ecosystem level although stress combination is probably more ecologically realistic in field. In the CROSTVOC (for CROp STress VOC) project, the impact of abiotic stresses (e.g. heat, drought, ozone and grazing) on BVOC emission will be investigated for field crops (maize and wheat) and grassland both at the ecosystem and plant scale. [less ▲]

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See detailPhysico-chemical properties and aroma profile of Acacia Honey produced in Romania
Madas, Mariana-Niculina ULg; Francis, Frédéric ULg; Marghitas, L.A. et al

in Communications in Agricultural and Applied Biological Sciences (2014, February 07), 79(1), 133-135

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See detailProfiles of the volatile organic compounds emitted by the masses of Abies nordmanniana somatic embryos at maintenance and maturation stages
Druart, Philippe; Michels, Franck ULg; Misson, Jean-Pierre et al

Poster (2014, February 07)

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See detailUse of lipases for the kinetic resolution of lactic acid esters in heptane or in a solvent free system
Richard, Gaetan ULg; Nott, Katherine; Nicks, François et al

Poster (2014, February 07)

This poster illustrates the kinetic resolution of lactic acid esters using CAL-B as catalyst. The racemic mixture is resolved in heptane and even in a solvent free system.

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See detailDevelopment of formulated elicitors to control bioagressors of wheat: which interests?
Le Mire, Géraldine ULg; Deleu, Magali ULg; Ongena, Marc ULg et al

Poster (2014, February 07)

Crop plants are constantly exposed to a wide range of environmental stresses that can cause major productivity loss. Fungicide treatments are the most efficient disease control methods used up to now ... [more ▼]

Crop plants are constantly exposed to a wide range of environmental stresses that can cause major productivity loss. Fungicide treatments are the most efficient disease control methods used up to now. However, the environmental and health issues caused by these chemicals make it urgent to develop safer methods for pathogen control. In the optic of a sustainable agriculture, several biocontrol tools have been developed during the last decades. Elicitors are increasingly subject to research as interesting alternatives to phytosanitary products. Yet, few efficient formulations exist on the market, and no product has been designed specifically to protect wheat crops. This study will thus focus on the the manipulation of host natural defenses through the use of elicitors as a preventive treatment against major wheat diseases such as Septoria tritici, Fusarium graminearum and Fusarium culmorum. [less ▲]

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See detailEffect of sun-drying on flavonoïd composition and antioxidant activity of three dark fig varieties
Bachir Bey, M.; Richard, Gaetan ULg; Fauconnier, Marie-Laure ULg et al

Poster (2014, February 07)

The aim of the study was to investigate the effect of sun-drying on individual flavonoïd and antioxidant activities of three dark fig varieties (Azenjar, Bouankik, and Aberkane). Flavonoïd compounds were ... [more ▼]

The aim of the study was to investigate the effect of sun-drying on individual flavonoïd and antioxidant activities of three dark fig varieties (Azenjar, Bouankik, and Aberkane). Flavonoïd compounds were identified and quantified using high-performance liquid chromatography. The results showed that all samples significantly differed in their flavonoïd compositions. [less ▲]

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See detailInteractions of a potential plant elicitor mannolipid with plant model membranes
Polo Lozano, Damien ULg; Lins, Laurence ULg; Ongena, Marc ULg et al

Poster (2014, February 07)

The use of chemical pesticides causes problems for human health and environment. In this context, there is an increasing interest for alternative products such as biopesticides. Among them, elicitors act ... [more ▼]

The use of chemical pesticides causes problems for human health and environment. In this context, there is an increasing interest for alternative products such as biopesticides. Among them, elicitors act on the plants by inducing systemic resistance against diseases caused by fungal, viral, bacterial agents and insects. The target of the elicitors is supposed to be the plant plasma membranes (PPM). The main mechanisms of interaction of many elicitors involve proteic receptors but lipid-based elicitors (LBE) may preferably interact with the lipidic fractions of PPM. However there is no detailed information at the molecular level on the PPM-LBE interactions. Our work is focused on a original synthetic LBE composed of a mannoside linked to a myristic acid. It has potential elicitor activities as shown by the assays on tobacco root cells. These activities could be related to its interaction with the lipidic phase of PPM. Since PPM are complex entities, the analyses of the PPM- molecule interactions are quite difficult. In this context, these interactions were carried out using biomimetic membranes of PPM such as Langmuir monolayers and multilayers. The effects of our molecule on these membranar systems were investigated by biophysical and in silico approaches. [less ▲]

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See detailUse of GC-MS in SIM-Scan mode for a selective quantification of polluting volatile organic compounds (VOC) in food industries
Eloundou Mballa, Pierre ULg; DETHIER, Bérénice ULg; Fauconnier, Marie-Laure ULg

Poster (2014, February 07)

The control of atmospheric emission discharges by food industries is classically achieved in two phases. The first one consists of sampling the target atmosphere followed by concentrating the volatile ... [more ▼]

The control of atmospheric emission discharges by food industries is classically achieved in two phases. The first one consists of sampling the target atmosphere followed by concentrating the volatile organic compounds (VOC) contained in a defined volume of this atmosphere on an adsorbing agent. These compounds are then thermally desorbed (TD) or desorbed with a solvent. In the second phase, the gas phase chromatogram (GC) will be coupled with mass spectrometry (MS) or flame ionization detector (FID) for compounds analysis. Quantitative analysis are usually achieved with solvent desorption followed by GC-FID. However, this mode imposes the dilution of the sample by a solvent and consequently may cause high detection thresholds as well as important matrix effect and possible interferences due to chemical reactions between analytes and the solvent. Besides, a preliminary qualitative analysis (GC-MS identification) is necessary. Combining TD-GC-MS allows very low detection thresholds to be reached, and the matrix effect and the interferences caused by products of the reactions between analytes and the solvent to be eliminated. The use of this combination in selected ion monitoring (SIM) mode allows targeting analytes via their characteristic ions, increasing their sensitivity and repeatability insuring more accuracy for simultaneous qualitative and quantitative analysis. This technique was experimented for seeking VOC in the atmosphere around food industries. [less ▲]

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See detailContribution to the study of alliinase, the active principle of garlic
DETHIER, Bérénice ULg; Ponchaux, Julien; Laloux, Morgan et al

Poster (2014, February 07)

Alliinase is a crucial enzyme in the Allium genus. The conversion of its substrates, cysteine sulfoxides, into volatile thiosulfinates is an important mechanism in the defence of the plant. It also ... [more ▼]

Alliinase is a crucial enzyme in the Allium genus. The conversion of its substrates, cysteine sulfoxides, into volatile thiosulfinates is an important mechanism in the defence of the plant. It also provides the typical pungent flavour of garlic. As a matter of fact, the thiosulfinates decompose in a range of organosulfur compounds (OSC) known for their biological activities (antioxidant, anticancer, anti-diabetes properties, etc.). The environment of the decomposition of the thiosulfinates determines the type and amount of products released. A deeper knowledge of the conditions that form each OSC could help us in the preparation of garlic-based nutraceutics in the perspective of healthier food consumption. This study aims to improve the extraction and purification the enzyme alliinase in order to perform the reaction between the enzyme and its substrates and assess the potentialities of garlic preparations. Three methods have been applied to evaluate the efficiency of the extractions and purifications of the enzyme. A first idea of the purity of the enzyme is given by an electrophoresis separation of each sample on a polyacrylamide gel (SDS-PAGE). The protein content is then measured by UV-vis spectrometry with Lowry-Folin reagent for the coloration and BSA as an external standard. Finally, the specific activity is assessed by an indirect measure of the pyruvate (released as a co-product): the addition of NADH and lactate dehydrogenase turns the pyruvate in lactate, and the disappearance of NADH is measured by UV-vis spectrometry at 340 nm. The extraction of the enzyme from garlic was performed either by PEG 8000 precipitation or by ammonium sulphate precipitation. Two purifications were tested: affinity chromatography (on ConA) and size-exclusion chromatography. The combination of the ammonium sulphate process with the ConA chromatography provided the purest enzyme, with the best activity but a lower yield than the size-exclusion process. Finally the stability of the enzyme has been assessed at 4, -20 and -80 °C, showing that the enzyme could be kept at -80 °C for over 4 months without deterioration, while activity loss was observed at higher temperature. [less ▲]

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See detailQuantitative gas chromatography - mass spectrometry profiling of volatile organic compounds produced by barley (Hordeum distichon L.) roots according to plant age
Delory, Benjamin ULg; Delaplace, Pierre ULg; du Jardin, Patrick ULg et al

Poster (2014, February 07)

In chemical ecology, the roles played by root-emitted volatile organic compounds (VOCs) in biotic interactions and the quantitative analysis of such chemicals in root tissues remain poorly documented. In ... [more ▼]

In chemical ecology, the roles played by root-emitted volatile organic compounds (VOCs) in biotic interactions and the quantitative analysis of such chemicals in root tissues remain poorly documented. In this context, this study aims at developing a fully automated analytical methodology allowing both identification and accurate quantification of VOCs produced by roots of a monocotyledon plant species. Briefly, VOC emitted by crushed barley roots are successively trapped by dynamic headspace sampling on Tenax TA adsorbents, thermally desorbed and cryofocused, separated by gas chromatography (GC) and finally analysed by mass spectrometry (MS) in both SCAN and selected ion monitoring modes. Results show that barley roots mainly produce four volatile aldehydes, namely hexanal, (E)-hex-2-enal, (E)-non-2-enal and (E,Z)-nona-2,6-dienal. These molecules are well-known linoleic (C18:2) and linolenic (C18:3) acid derivatives produced via the lipoxygenase and the hydroperoxide lyase pathways of higher plants. Our findings contrast with analyses documented on aboveground barley tissues that mainly emit C6 aldehydes, alcohols and their derivative esters. Moreover, preliminary results indicate quantitative changes in the volatile profile contained in barley roots according to plant age. Multivariate statistical analyses are currently underway to quantitatively assess these changes using plants at five selected developmental stages ranging from germination to the end of tillering. [less ▲]

Detailed reference viewed: 49 (11 ULg)