References of "Fauconnier, Marie-Laure"
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See detailSalinity impact on seed yield, polyphenols composition and antioxidant activity of Fennel (Foeniculum vulgarae Mill) extracts
Bettaieb ép Rebey, Ines ULiege; Bourgou, S.; Marzouk, B. et al

in Journal of new sciences (in press)

Recently, medicinal and aromatic plants have received much attention in several fields such agroalimentary, perfumes, pharmaceutical industries and natural cosmetic products. The consumption of herbal ... [more ▼]

Recently, medicinal and aromatic plants have received much attention in several fields such agroalimentary, perfumes, pharmaceutical industries and natural cosmetic products. The consumption of herbal medicines is widespread and is continuously increasing worldwide. Although, secondary metabolites in the medicinal and aromatic plants were fundamentally produced by genetic processing but, their biosynthesis is strongly influenced by environmental factors. Salinity is one of the major factors that affect essential oil biosynthesis and secretion (Heuer et al. 2002). In Tunisia, salt-affected soils cover about 10% of the total area of the country. Salinity led to biochemical disorders and can change plant behaviour regarding the biosynthesis of primary and secondary metabolites. Among all the secondary metabolites synthesized by plants, phenolic compounds are some of the most widespread. In this context, this research evaluated the effect of salinity on total and individual polyphenols contents as well as the antioxidant activities of fennel (Foeniculum vulgarae Mill.) seeds of two geographic origins, Tunisia (TFS) and Egypt (EFS). This plant is one of the most common aromatics in the Mediterranean kitchen. Plants were treated with different concentrations of NaCl treatment: 0, 50, and 75 mmoL. Plant growth was significantly reduced with the severity of saline treatment. This also caused important reductions in the seed yield and yield components. Besides, total polyphenol content was higher in treated seeds, and salinity improved the amount of individual phenolic compounds. Moreover, antioxidant activities of the extracts were determined by four different test systems, namely 2,2-diphenyl-1-picrylhydrazyl, β-carotene/linoleic acid chelating, and reducing power assays. The highest antioxidant activities were reveled in severe stressed plants for both TFS and EFS. In this case, fennel seeds produced under saline conditions may function as a potential source of antioxidant compounds, which could support the utilization of this plant in a large field of applications such as food industry. [less ▲]

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See detailComparative assessment of phytochemical profiles and antioxidant properties of Tunisian and Egyptian anise (Pimpinella anisum L.) seeds
Bettaieb ép Rebey, Ines ULiege; Bourgou, Soumaya; Aidi Wannes, Wissem et al

in Plant Biosystems (in press)

Anis (Pimpinella anisum L.) seeds obtained from two geographic origins Tunisia (TAS) and Egypte (EAS) were studied regarding their biochemical composition and the antioxidant potential of their extracts ... [more ▼]

Anis (Pimpinella anisum L.) seeds obtained from two geographic origins Tunisia (TAS) and Egypte (EAS) were studied regarding their biochemical composition and the antioxidant potential of their extracts. The results showed that the highest value of oil was detected with TAS compared to that of EAS ones. Ten (10) fatty acids were identified for the two locations and petroselinic acid was the most prevalent in oil seeds and interestingly, TAS displayed a significantly higher level of this acid than EAS. Besides, TAS exhibited slightly higher essential oil yield than the Egyptian variety and that trans-anethole was the dominant for the two provenances. In both accessions, the highest total phenolic, flavonoid and tannin content was obtained with ethyl acetate fraction. Therefore, TAS exhibited higher chelating and reducing powers than EAS which may be due to a slightly different phenolic composition between the two accession seed extracts. The phenolic compositions of TAS and EAS revealed that ethyl acetate extracts showed higher proportions of naringin, chlorogenic acid and rosmarinic acid. However, ethanol extracts were richer in larcitrin, rosmarinic acid and cirsimartin. The overall results revealed that aniseeds might constitute a novel source of natural antioxidants and could be used as food additive. [less ▲]

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See detailInfluence of different hydrocolloids on dough thermo-mechanical properties and in vitro starch digestibility of gluten-free steamed bread based on potato flour
Liu, Xingli ULiege; Mu, Taihua; Sun, Hongnan et al

in Food Chemistry (2018), 239

The effects of hydrocolloids (Hydroxypropylmethylcellulose (HPMC), Carboxymethylcellulose (CMC), Xanthan gum (XG), and Apple pectin (AP)) at different concentrations on dough thermo-mechanical properties ... [more ▼]

The effects of hydrocolloids (Hydroxypropylmethylcellulose (HPMC), Carboxymethylcellulose (CMC), Xanthan gum (XG), and Apple pectin (AP)) at different concentrations on dough thermo-mechanical properties and in vitro starch digestibility of gluten-free potato steamed bread were investigated. Results showed that hydrocolloids addition significantly increased the gelatinization temperature (from 52.0 to 64.2 °C) and water absorption (from 56.22 to 66.50 %) of potato dough. Moreover, hydrocolloids may be interacted with protein and starch, the density of potato protein bands was decreased by hydrocolloids addition, the reason might be that higher molecular weight complexes might be formed between proteins-hydrocolloids or proteins-proteins, thus change the protein solubility. Furthermore, steamed breads with hydrocolloids presented higher specific volume and lower hardness, and the rapidly digestible starch and estimated glycemic index were significantly decreased from 45.51 to 20.64, from 69.54 to 55.17, respectively. In conclusion, HPMC and XG could be used as improvers in the gluten-free potato steamed bread. [less ▲]

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See detailEco-extraction of aniseed oil: Use of green solvent as alternative solvent to n-hexane
Bettaieb ép Rebey, Ines ULiege; Bourgou, Soumaya; Detry, Pauline ULiege et al

Poster (2017, November 25)

Pimpinella anisum L. (anise) seeds have been widely used as a culinary ingredient as well as traditional remedies for the treatment of different disorders in the folk medicine systems of different ... [more ▼]

Pimpinella anisum L. (anise) seeds have been widely used as a culinary ingredient as well as traditional remedies for the treatment of different disorders in the folk medicine systems of different civilizations. Its essential oil show many properties and is already well studied and used. The lipid fraction of the seeds has also already been investigated. Petroselinic acid was the most prevalent fatty acid in anis oil seeds. This one also presents several properties. Nowadays, fixed oils are extracted with the help of petrosolvents. This raise issues regarding environment and security, but also public health. Regulations are more and more strict and alternative more and more searched. In general, those ones consist in the use of substitution solvents. Thus, the purpose of this work was designed to evaluate the performances of a green solvent compared to n-hexane in aniseed oil extraction. [less ▲]

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See detailLes arbres, ces natures sensibles : Oui, les plantes se parlent
Fauconnier, Marie-Laure ULiege

Article for general public (2017)

Marie-Laure Fauconnier, professeur ordinaire à Gembloux Agro-Bio Tech, ULg. Chimiste, elle mène des recherches dans le domaine des molécules naturelles volatiles émises par le règne végétal.

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See detailUsing phenotyping and complementary VOC profiling approach to investigate physiological response of Brassica napus L. plantlets under cadmium and epoxiconazole abiotic stresses
Durenne, Bastien ULiege; Geerts, Pascal; Druart, Philippe et al

Poster (2017, November)

Thanks to a laboratory, high-throughput and sterile homemade glass chambers system giving us the capacity to follow VOC (Volatile Organic Compounds) emissions at any time during plant growth, we are able ... [more ▼]

Thanks to a laboratory, high-throughput and sterile homemade glass chambers system giving us the capacity to follow VOC (Volatile Organic Compounds) emissions at any time during plant growth, we are able to compare the phenotype of the plant and its VOC emission in the same time. Although the system could be used for biotic stress too, we decided to investigate the physiological response of oilseed rape plantlets such as model plant concerning two atypical abiotic stresses: cadmium and epoxiconazole. Experiments were achieved under several concentrations corresponding to a gradient from a tolerant to a sublethal dose. Oilseed rape plantlets phenotyping consisted in daily symptoms observation (chlorosis for cadmium stress and shorter internodes length for expoxiconazole stress), shoots and roots length recording and finally, fresh and dry weight assessment. The main goal was therefore to analyse VOC profile with a non-destructive method in parallel to this phenotype characterization in absence of interferences with uncontrolled environmental conditions. Cadmium was chosen because it can be problematic within agricultural soils (phosphate fertilizers among other things) and epoxiconazole because it is a systemic fungicide used in cereals known to be hugely persistent into agricultural soils. [less ▲]

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See detailEnvironmental controls of methanol emissions from a grazed grassland in Dorinne, Belgium
Michel, Colin ULiege; Heinesch, Bernard ULiege; Bachy, Aurélie ULiege et al

Poster (2017, October 20)

Despite the growing interest for oxygenated volatile organic compounds (OVOC) over the last 15 years due to their role in the atmospheric chemistry, current knowledge about OVOC exchanges by grassland and ... [more ▼]

Despite the growing interest for oxygenated volatile organic compounds (OVOC) over the last 15 years due to their role in the atmospheric chemistry, current knowledge about OVOC exchanges by grassland and the environmental factors driving them remains lacunar. However, those ecosystems represent an important part of the total earth surface (13.37%). This study conducted on a grazed grassland therefore aims to quantify OVOC exchanges over full grazing seasons in order to understand the mechanisms behind these exchanges. It took place within the activities of the CROSTVOC project (CROp Stress VOC) and therefore gives an important attention to the stress induced fluxes. BVOC flux measurements were performed with a PTR-MS for the measurements of OVOC mixing ratios on two different scales: the eddy Covariance method was used during two whole grazing seasons (2014 and 2015) and in the 2016 summer, measurements were also performed on a smaller scale by using all-teflon automated dynamic chambers. The chambers allowed measuring accurately the impact of grazing by following simultaneously undisturbed and grazed grassland patches. This study pointed out that several OVOC were exchanged in variable quantities, with methanol being by far the most important. Methanol fluxes exhibited a clear diurnal cycle with close-to-zero fluxes at night and maximum fluxes at midday. The flux was also much larger in the summer than during autumn or spring. For the eddy Covariance data, the average methanol flux in the summer (0.033 μg.m-2s-1) was in the same range of other studies, being lower than the average found by Bamberger et al. in 2010 (0.080 μg.m-2s-1) and by Ammann (0.077 μg.m-2s-1). Fluxes from the chambers were slightly lower with an average value of 0.27 μg.m-2s-1 but they were measured in the late summer. Driver analysis is still ongoing but first results showed that the flux was strongly correlated at short time scale (half hourly for the EC method and one and a half hourly for the chambers) to the photosynthetic photon flux density and the latent heat flux. We believe that their influence comes mainly from the control practiced by stomatal conductance in non-steady state conditions and expansion of cell walls in steady state conditions. The cuvette data showed that grazing resulted in significantly enhanced emissions of methanol that lasted for several days. On the contrary, the eddy Covariance method cannot distinguish between grazed and non-grazed grass and only a small fraction of the method footprint had been grazed the previous days on average. Our eddy-covariance data suggested that an increase in the stocking density did not contribute to a significant rise of methanol emission. The limited variability in stocking density during the two growing seasons and the more powerful effect of other drivers likely induced that the flux variability due to the SD was too low to be measured by the eddy Covariance method. In depth comparisons between the eddy-covariance data and the dynamic chambers data will be carried out. [less ▲]

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See detailAssessment of antioxidant potential of phenolic compounds fractions of Algerian Ceratonia siliqua L. pods during ripening stages
Ydjedd, Siham; Chaala, Makhlouf; Richard, Gaetan ULiege et al

in International Food Research Journal [=IFRJ] (2017), 24(5), 2041-2049

The consecutive extraction via different solvents of increasing/decreasing polarity, which allowed a pre-fractionation of the extracted compounds, is widely used as major method for extraction of ... [more ▼]

The consecutive extraction via different solvents of increasing/decreasing polarity, which allowed a pre-fractionation of the extracted compounds, is widely used as major method for extraction of polyphenols. Thus, the phenolic compounds of Ceratonia siliqua L., at unripe and ripe stages, were extracted by four solvents of increasing polarity (hexane, chloroform, ethyl acetate, and acetone/water (70:30, v/v), consecutively. The extracts were evaluated for their total phenolic and flavonoid contents, while the antioxidant capacity was assessed in vitro using DPPH radical and hydrogen peroxide (H2O2) scavenging assays. The phenolic profile has been carried out by HPLC-MS/MS. The results showed that the phenolic contents and antioxidant capacity varied with the nature of extracts and the ripening stage. The 70% aqueous acetone and the ethyl acetate extracts contained the highest phenolic and flavonoid contents, and subsequently showed a pronounced scavenging activity on DPPH and hydrogen peroxide at unripe and ripe stages. The HPLC-MS/MS allowed the identification of five free phenolic acids (gallic, syringic, cinnamic, p-coumaric and ellagic acid for the first time) and five flavonoids (apigenin, naringenin, kaempferol, quercetin rhamnoside and myricetin rhamnoside) at both ripening stages. [less ▲]

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See detailCould indolic glucosinolates root profiling be correlated to Brassica napus L. cadmium stress tolerance?
Durenne, Bastien ULiege; Blondel, Alodie; Druart, Philippe et al

Poster (2017, September)

Glucosinolates profiling among Brassicaceae and Brassica napus L. especially is largely studied regarding biotic stress. Nevertheless literature review could lead to suppose that theses secondary ... [more ▼]

Glucosinolates profiling among Brassicaceae and Brassica napus L. especially is largely studied regarding biotic stress. Nevertheless literature review could lead to suppose that theses secondary metabolites hugely produced within Brassicaceae family can interact in regards to cadmium stress as well. Sulfate primary metabolism plays an important role to Brassica napus L. cadmium stress tolerance and glucosinolates could serve as sulfate pool under such stress conditions. We describe here an in vitro experiment performed under sterile and controlled conditions showing the relationship between Brassica napus L. var. Es Astrid tolerance to a low concentration of cadmium and a decrease of a major indolic glucosinolates produced by the roots of this variety. Phenotyping results obtained from these cadmium stress conditions confirm also that Brassica napus L. remains a highly cadmium tolerant species probably due to glucosinolates root metabolism particularly. [less ▲]

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See detailNew Alternatives to Chemical Pesticides: Deciphering the Action Mechanisms of Lipid based Plant Elicitors via Complementary Biophysical and Biological Approaches
Fauconnier, Marie-Laure ULiege

Scientific conference (2017, July)

An elicitor may be defined as any molecule produced by pathogenic or non-pathogenic microorganisms or by host plants (synthesizing or accumulating the molecule) capable of inducing or promoting the ... [more ▼]

An elicitor may be defined as any molecule produced by pathogenic or non-pathogenic microorganisms or by host plants (synthesizing or accumulating the molecule) capable of inducing or promoting the natural resistance of plants to one or more pathogens, which resistance may be local or systemic. [less ▲]

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See detailFirst Characterization of the Volatile Organic Compounds Emitted by Banana Plants (Musa sp.)
Berhal, Chadi ULiege; De Clerck, Caroline ULiege; LEVICEK, Carolina et al

Conference (2017, June 16)

Banana fruit (Musa sp.) ranks fourth in developing world's production, and has economical and nutritional key values. The popular and most dominant variety of the dessert banana group is the Cavendish ... [more ▼]

Banana fruit (Musa sp.) ranks fourth in developing world's production, and has economical and nutritional key values. The popular and most dominant variety of the dessert banana group is the Cavendish variety, and Plantain represents that status for the cooking banana group. Despite the importance of the Volatile Organic Compounds (VOCs), which are secondary metabolites with a high vapour pressure, in their utility in the plant protection and communication processes, they were never documented for the plant itself. Thus, the aim of this PHD thesis is to study the VOCs emitted by the plant, rather than their fruits or flowers. A protocol was optimized for the extraction of the banana plant's VOCs. The results of the first analysis showed 11 VOCs for the Cavendish, mainly (E,E)-α-farnesene (87.90 ± 11.28 ng/µl), methyl salicylate (33.82 ± 14.29) and 6-methyl-5-hepten-2-one (29.60 ± 11.66), and 14 VOCs for the Pacific Plantain cultivars, mainly (Z,E)-α-farnesene (799.64 ± 503.15),(E,E)-α-farnesene (571.24 ± 381.70) and (E) β ocimene (241.76 ± 158.49). Most of these compounds belong to the terpenes group (8 for Cavendish, 10 for Pacific Plantain). The other compounds detected were ketones, esters and aldehydes. Eight compounds were common between the two varieties (myrcene, Z and E β-ocimene, 6-methyl-5-hepten-2 one, 6-methyl-3,5-hepadien-2-one, a-farnesene, methyl salicylate and β-ionone). This exploratory study paves the way for an in-depth characterisation of VOCs emitted by Musa plants. [less ▲]

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See detailEnvironmental controls of biogenic volatile organic compound emissions from a grazed grassland in Dorinne, Belgium
Michel, Colin ULiege; Heinesch, Bernard ULiege; Bachy, Aurélie ULiege et al

Poster (2017, June 15)

Despite the growing interest for oxygenated volatile organic compounds (OVOC) over the last 15 years due to their role in the atmospheric chemistry, current knowledge about OVOC exchanges by grassland and ... [more ▼]

Despite the growing interest for oxygenated volatile organic compounds (OVOC) over the last 15 years due to their role in the atmospheric chemistry, current knowledge about OVOC exchanges by grassland and the environmental factors driving them still entails gaps. However, those ecosystems represent an important part of the total earth surface (13.37%). This study conducted on a grazed grassland therefore aims to quantify OVOC exchanges over full grazing seasons in order to understand the mechanisms behind these OVOC exchanges. The Eddy Covariance method was used for flux quantification, with a PTR-MS for the measurements of OVOCs mixing ratios. It took place within the activities of the CROSTVOC project (CROp Stress VOC) and therefore gives an important attention to the stress induced fluxes. BVOC flux measurements were also performed on a smaller scale by using all-teflon automated dynamic chambers covering a surface of 0.1 m² each. [less ▲]

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See detailOral communication: Brassica napus L. plantlets affected during in vitro growth by the presence of epoxiconazole.
Durenne, Bastien ULiege; Blondel, Alodie; Ducat, Nathalie et al

Conference (2017, May 24)

Detailed reference viewed: 36 (6 ULiège)
See detailStudy of volatile organic compounds active against barley pathogens
De Clerck, Caroline ULiege; Kaddes, Amine ULiege; Fiers, Marie et al

Conference (2017, May 23)

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See detailStudy of VOCs in the interaction between Banana and Foc TR4
Berhal, Chadi ULiege; De Clerck, Caroline ULiege; LEVICEK, Carolina et al

Poster (2017, May 23)

Banana fruit (Musa sp.) ranks fourth in term of worldwide production, and has economical and nutritional key values. The popular and most dominant variety of the dessert banana group is the Cavendish ... [more ▼]

Banana fruit (Musa sp.) ranks fourth in term of worldwide production, and has economical and nutritional key values. The popular and most dominant variety of the dessert banana group is the Cavendish variety. Nowadays, the Cavendish is endangered by the newly emergent race of the Panama disease, caused by the fungal pathogen Fusarium oxysporum f.sp. cubense TR4 (Foc TR4). Despite the importance of the Volatile Organic Compounds (VOCs), which are secondary metabolites with a high vapour pressure, in their utility in the plant protection processes, they were never documented as a way to manage this disease on Cavendish. Thus, the aim of this PhD thesis is to study the VOCs in the specific interaction Cavendish/Foc TR4, as a way to manage this threat. Based on the work previously done with other plants at Gembloux Agro-Bio Tech/University of Liège/Belgium, a protocol was optimized for the extraction of the banana plant’s VOCs. And in parallel, models of in-vitro and in-vivo inoculations are under development, in order to distinguish the root zone from the upper part of the plant in the study. The results of the first analysis showed that the majority of the Cavendish VOCs belongs to the terpenes group, as well as ketones and an organic ester. The identified key VOCs of the interaction will be subject to toxicity tests, in order to determine their effect on the development of the plant and the pathogen. The banana plant is a staple food for more than 400 Million people in the world, while this disease persists as a major threat for its production, and this original study could contribute to the fight against this threatening disease. [less ▲]

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