References of "Fauconnier, Marie-Laure"
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See detailDevelopment of a new method for arabidopsides extraction and purification
Genva, Manon ULg; Andersson, Mats X; Nasir, Mehmet Nail ULg et al

Poster (2016, November 16)

In the present work, a new method for arabidopsides extraction and purification was developed.

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See detailStudy of the volatile organic compounds in the interaction between banana plants and the fungal disease Fusarium oxysporum f.sp. cubense Tropical Race 4 (FocTR4)
Berhal, Chadi ULg; De Clerck, Caroline ULg; LEVICEK, CAROLINA et al

Conference (2016, October 11)

Banana fruit (Musa sp.) ranks fourth in term of worldwide production, and has economical and nutritional key values. Cavendish is the popular and most dominant variety of the dessert banana group ... [more ▼]

Banana fruit (Musa sp.) ranks fourth in term of worldwide production, and has economical and nutritional key values. Cavendish is the popular and most dominant variety of the dessert banana group. Nowadays, it is endangered by the emergent race of the Panama disease, caused by Fusarium oxysporum f.sp. cubense TR4 (Foc TR4). Despite the importance of the Volatile Organic Compounds (VOCs), which are secondary metabolites with a high vapour pressure, and their role in plant protection, they have never been documented as a way to manage this disease on Cavendish. Thus, the aim of this PhD thesis is to study the VOCs in the specific interaction Cavendish/Foc TR4, as a way to manage this threat. Based on the work previously done with other plants at Gembloux Agro-Bio Tech, a protocol was optimized for the extraction of the banana plant’s VOCs. In parallel, models of in-vitro and in-vivo inoculations are under development to distinguish the VOCs emitted by the root zone and those emitted by the aerial part of the plant. The results of the first analysis showed that the majority of the Cavendish VOCs belongs to the terpenes group, as well as ketones and an organic ester. The VOCs specifically emitted during the plant-pathogen interaction will be studied more in depth by evaluating their effect on the plant and the pathogen. At long term, and depending on the results, agro-ecological applications of this knowledge could include breeding for new variety or combining banana with other plants expressing VOCs against Foc TR4, applying VOCs on field to stimulate plant defences or to contain the pathogen. [less ▲]

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See detailInvestigating the effect of plant-derived amendments on PAHs degradation in brownfield contaminated soils
Davin, Marie ULg; Starren, Amandine ULg; Colinet, Gilles ULg et al

Conference (2016, September 13)

Polycyclic aromatic hydrocarbons (PAHs) are a class of persistant organic compounds of major concern that tend to accumulate in the environment, damaging ecosystems and health. Brownfields represent an ... [more ▼]

Polycyclic aromatic hydrocarbons (PAHs) are a class of persistant organic compounds of major concern that tend to accumulate in the environment, damaging ecosystems and health. Brownfields represent an important tank for PAHs that require remediation. As an alternative to environmentally aggressive, expensive and often disruptive soil remediation strategies, experiences have been carried on to understand and develop techniques based on bioremediation and phytoremediation. PAHs degradation experiments were conducted in microcosms (laboratory scale) in order to determine whether several plant-derived amendments could enhance bioremediation. Briefly, samples of aged contaminated soils were treated with different concentrations of Medicago sativa or Trifolium pratense root exudates or dried roots, commercial saponin, a natural surfactant found in some plant roots such as some Fabaceae, and some samples were left unamended as controls. Soil samples were incubated for two and four weeks at controlled temperature (28°C). Carbon dioxide emission was monitored throughout the whole incubation. At the end of each experiment, dehydrogenase activity was measured as an indicator of microbiological activity and residual PAHs were determined using High-Performance Liquid Chromatography with Fluorimetric Detection (HPLC-FLD). In total, eleven amendment modalities and two incubation periods were tested and repeated four times. Preliminary experiments show promising results as amended samples seem to show different respiration activities. Ongoing studies will allow discussion as to whether or not PAHs degradation is influenced by the different modalities and if there are any differences according to the nature and concentration of the amendment. [less ▲]

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See detailComparative study of nutritional quality of potato-wheat steamed and baked breads made with four cultivated potato flour
Liu, Xingli; Mu, Tai-Hua; Sun, Hongnan et al

in International Journal of Food Sciences & Nutrition (2016)

Bread is traditional staple food in western and oriental countries varying in both the ingredients used, preparation methods, and heating methods applied, which are mainly used as an energy source because ... [more ▼]

Bread is traditional staple food in western and oriental countries varying in both the ingredients used, preparation methods, and heating methods applied, which are mainly used as an energy source because of high carbohydrate. Baked bread is popular in western countries. While steamed bread is a type of fermented and steamed wheat-based food of Chinese origin, and represents ∼40% of the wheat consumption in China. [less ▲]

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See detailPlant esterified oxylipins: structure – function relationship
Genva, Manon ULg; Andersson, Mats X.; Nasir, Mehmet Nail ULg et al

Poster (2016, September)

In the present work, high quantities of arabidopsides were extracted and purified from Arabidopsis thaliana L.

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See detailSOLINDIC : Etude de l’activité biologique du système « Sol-Plante » en vue de préserver les fonctionnalités des sols agricoles
Blondel, Alodie; Durenne, B.; Mingeot, D. et al

Report (2016)

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See detailInvestigating the Effect of Plant Root Exudates and of Saponin on Polycyclic Aromatic Hydrocarbons Solubilization in Brownfield Contaminated Soils
Davin, Marie ULg; Fauconnier, Marie-Laure ULg; Colinet, Gilles ULg

Conference (2016, August 08)

In Wallonia, there are 6,000 estimated brownfields (rising to over 3.5 million in Europe) that require remediation. Polycyclic Aromatic Hydrocarbons (PAHs) are a class of recalcitrant carcinogenic ... [more ▼]

In Wallonia, there are 6,000 estimated brownfields (rising to over 3.5 million in Europe) that require remediation. Polycyclic Aromatic Hydrocarbons (PAHs) are a class of recalcitrant carcinogenic/mutagenic organic compounds of major concern as they accumulate in the environment and represent 17% of all encountered pollutants. As an alternative to environmentally aggressive, expensive and often disruptive soil remediation strategies, a lot of research has been directed to developing techniques targeting organic pollutants. The following experiment, based on the observation that PAHs soil content decreases in the presence of plants, aimed at improving our understanding of the underlying mechanisms involved in phytoremediation. It focusses on plant root exudates and whether they improve PAHs solubilization, which would make them more available for bioremediation by soil microorganisms. The effect of saponin, a natural surfactant found in some plant roots such as members of the Fabaceae family, on PAHs solubilization was also investigated as part of the implementation of the experimental protocol. The experiments were conducted on soil collected from a brownfield in Saint-Ghislain (Belgium) and presenting weathered PAHs contamination. Samples of soil were extracted with different solutions containing either plant root exudates or commercial saponin. Extracted PAHs were determined in the different aqueous solutions using High-Performance Liquid Chromatography and Fluorimetric Detection (HPLC-FLD). Both root exudates of alfalfa (Medicago sativa L.) or red clover (Trifoliumpratense L.) and commercial saponin were tested in different concentrations. Distilled water was used as a control. First of all, results show that PAHs are more extracted using saponin solutions than distilled water and that the amounts generally rise with the saponin concentration. However, the amount of each extracted compound diminishes as its molecular weight rises. Also, it appears that passed a certain surfactant concentration, PAHs are less extracted. This suggests that saponin might be investigated as a washing agent in polluted soil remediation techniques, either for ex situ or in situ treatments, as an alternative to synthetic surfactants. On the other hand, preliminary results on experiments using plant root exudates also show differences in PAHs solubilization compared to the control solution. Further results will allow discussion as to whether or not there are differences according to the exudates provenance and concentrations. [less ▲]

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See detailGamifier un quiz – Effet graduel sur la performance, l’immersion et la perception de compétence
le Maire, Nathalie ULg; Fauconnier, Marie-Laure ULg; Colaux, Catherine ULg et al

Conference (2016, July 06)

Dans un contexte marqué par l’arrivée d’une génération pour laquelle les technologies, les réseaux sociaux et les jeux en ligne relèvent de l’évidence (Stein, 2013; Weiler, 2004), le jeu a souvent été ... [more ▼]

Dans un contexte marqué par l’arrivée d’une génération pour laquelle les technologies, les réseaux sociaux et les jeux en ligne relèvent de l’évidence (Stein, 2013; Weiler, 2004), le jeu a souvent été qualifié de véhicule d’une pédagogie rendant l’étudiant acteur de son apprentissage, conférant une dimension de défi aux activités éducatives et générant une motivation intrinsèque (Foster, 2008; Kang & Tan, 2008; McFarlane et al., 2002; Mitchell & Savill-Smith, 2004; Papastergiou, 2009). Ce potentiel éducatif du jeu a cependant été souvent associé à des jeux immersifs (de Freitas, 2006) requérant la mise en œuvre d’une trame narrative et dont le développement peut s’avérer couteux. L’utilisation de mini-jeux est considérée comme une solution alternative à ces « serious games » complexes car ils permettent d’enseigner un grand nombre de concepts tout en générant de faibles coûts de développement (Illanas, Gallego, Satorre, & Llorens, 2008). Ceux-ci présentent des règles basiques, sont faciles à jouer et conçus comme objets d’apprentissage de sorte qu’il soit aisé pour l’étudiant de percevoir les informations essentielles et que leur pratique soit bénéfique pour l’apprentissage (Frazer, Argles, & Wills, 2007). La gamification, à savoir la transposition des principes issus de l’univers du jeu au domaine de l’éducation (Deterding, Dixon, Khaled, & Nacke, 2011), ne porte donc pas ici sur des jeux de simulation immersifs mais vise plus réalistement la transposition à des activités d’apprentissage d’un certain nombre de principes et de leviers à l’œuvre dans des mini-jeux populaires tel que Candy Crush. La question de recherche qui a guidé l’expérience décrite ci-après concerne l’évaluation du potentiel pédagogique de l’utilisation d’un mini-jeu de ce type dans un cours de chimie générale en complément des supports pédagogiques plus classiquement utilisés en première année d’université. Concrètement, ce mini-jeu appelé « Atomica » se présente comme un quiz d’entrainement portant sur un chapitre réputé difficile du cours, l’atomistique. Ce quiz a fait l’objet de quatre versions activant chacune un nombre croissant d’éléments de gamification (indiqués en gras) décrits par plusieurs auteurs comme étant les « ingrédients » ou « building blocks » d’un bon jeu (Bunchball Inc., 2010; Dignan, 2011; Reeves & Read, 2013) : - La version contrôle du QCM se présente comme similaire aux évaluations formatives couramment utilisées à l’université ; - La version 1 segmente le QCM en six niveaux de difficulté ; - La version 2 ajoute un feedback automatisé aux niveaux de difficulté ; - La version 3 présente une version maximaliste de la gamification puisque, aux éléments précédents, elle ajoute un compte à rebours, des indices dont l’appel engendre une perte de points, un classement des meilleurs joueurs et un indicateur social (possibilité de se situer par rapport à la communauté de joueurs). Ce choix d’un dispositif graduel se justifie notamment par l’intérêt pour la détection d’un seuil à partir duquel l’ajout d’éléments de gamification fait basculer la perception d’un quiz habituel vers un artefact relevant du mini-jeu et générant ainsi un état de flow chez le joueur. Chaque version a été soumise à un groupe d’une trentaine d’étudiants, selon une procédure expérimentale contrôlée, avec pour objectif la collecte de données (en cours) sur : - L’état de flow ou expérience optimale engendré par chacune des versions (orientation comparative) défini comme un état subjectif de bien-être (Csikszentmihalyi, 1990) engendrant une immersion totale dans l’activité. Afin de mesurer le flow, une échelle spécifique aux jeux éducatifs (EGameFlow) développée en 2009 (Fu, Su, & Yu, 2009) a été utilisée dans le cadre de cette étude. - Une des trois composantes du modèle de la dynamique motivationnelle de Viau, la perception du sentiment de compétence (Bandura, 1993; Pajares, 2014) considéré comme un moteur essentiel à l’apprentissage et un déterminant de l’intérêt pour une discipline scolaire. - La performance à un test de connaissance soumis aux étudiants avant et après l’expérience de jeu. Les motivations sous-tendant le processus d’exploration des mini-jeux sont à chercher du côté d’un certain discours pédagogique qui prône l’inscription d’une dimension ludique dans les apprentissages en enseignement supérieur. Nourrie par ses résultats expérimentaux, la communication proposera un retour réflexif plus général sur la valeur, les limitations et les conditions de réalisation de ce discours. [less ▲]

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See detailCaractérisation des mécanismes impliqués dans la perception de rhamnolipides naturels et synthétiques chez Arabidopsis thaliana
Luzuriaga Loaiza, Walter ULg; Schellenberger, Romain; Obounou Akong, Firmin et al

Scientific conference (2016, July 05)

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See detailScreening of mahoran plants for cosmetic applications.
Saive, Matthew ULg; Frederich, Michel ULg; Fauconnier, Marie-Laure ULg

Poster (2016, June 01)

This study’s main aim is to identify plant species showing anti-oxidant, anti-inflammatory and skin whitening properties. A selection of 89 samples from previous infield studies on the traditional uses of ... [more ▼]

This study’s main aim is to identify plant species showing anti-oxidant, anti-inflammatory and skin whitening properties. A selection of 89 samples from previous infield studies on the traditional uses of plants in Mayotte were analyzed. The anti-oxidant activity was assessed using the α, α-diphenyl-β-picrylhydrazyl (DPPH) method developed by Blois [1]. The anti-inflammatory activity and the skin whitening activity were both assessed through the study of inhibition kinetics from specific enzymes: respectively, lipoxygenase (EC 1.13.11.12) due to its important role in the leukotriene pathway and tyrosinase (EC. 1.14.18.1) as it takes part in the melanogenesis pathway. The IC 50 value for each sample and for each activity was obtained using UV/Vis spectrophotometric technics. These IC 50 were then compared with the ones obtained from known molecules found in the literature: namely, (±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (IC50 = 4,388 µM) for the anti-oxidant activity, nordihydroguaiaretic acid (IC50 = 3,5µM) for the anti-inflammatory activity and kojic acid (IC50 = 722 µM) for the anti-tyrosinase activity. The most effective anti-oxidant activity was observed in fresh leaves from Acalypha wilkesiana Müll.Arg. (IC50 = 0,381 g/L) and Leea guineensis G. Don. (IC50 = 0,225 g/L) as well as in dried roots from Litchi chinensis Sonn. (IC50 = 0,346 g/L). All results are expressed in grams of fresh matter. According to the preliminary tests for the-anti-inflammatory and skin whitening activities, the crude extracts from Kalanchoe pinnata Lam. Pers., Litchi chinensis Sonn., Zingiber zerumbet (L.) Sm., Leea guineensis G. Don. and Paullinia pinnata L. are likely to show promising activities. [less ▲]

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See detailGlucosinolate profiles by HPLC-DAD, phenolic compositions and antioxidant activity of Eruca vesicaria longirostris: Impact of plant part and origin
Bouacida, Saoussen; Koubaier, Hayet Ben Haj; Snoussi, Ahmed et al

in Mediterranean Journal of Chemistry (2016), 5(5), 528-539

The glucosinolate profiles, phenol and flavonoid contents and the antioxidant activity of Eruca vesicaria longirostris were studied for different organs and origins. Eleven desulpho-glucosinolates (DS ... [more ▼]

The glucosinolate profiles, phenol and flavonoid contents and the antioxidant activity of Eruca vesicaria longirostris were studied for different organs and origins. Eleven desulpho-glucosinolates (DS-GLSs) were isolated and quantified by lipid chromatography- DAD. Similarity between profiles was obtained. Total DS-GLS content, expressed as sinigrin equivalents (SE) revealed a certain variabilily ranging between (76.07-45.61), (27.01-13.53), (4.52 -18.01), (9.39-3.37) and (1.16-13.99) μmol /g DW for seeds, flowers, leaves, roots and stems, respectively. [less ▲]

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See detailDisentangling soil from plant methanol exchanges in a maize field: a first step
Bachy, Aurélie ULg; Mozaffar, Ahsan ULg; Aubinet, Marc ULg et al

Poster (2016, June)

This poster presents the current research done in order to disentangle soil from plant methanol exchanges in a maize field. Methanol exchanges were calculated at ecosystem-scale (therefore including both ... [more ▼]

This poster presents the current research done in order to disentangle soil from plant methanol exchanges in a maize field. Methanol exchanges were calculated at ecosystem-scale (therefore including both components). It bases on the following observation: methanol exchanges on bare soil (measured when maize was at germination stage) were as important as when plants were fast growing (and thereby, when the highest methanol plant methanol emissions were expected), and this under similar weather conditions. The goal of this poster is thus to understand why emissions were similar at these two periods. First, it addresses the question of the actual contribution of maize plants in methanol exchanges, by comparing up-scaled methanol exchanges measured on maize at leaf-scale (Mozaffar A.) to those measured at ecosystem-scale. Then, it investigates methanol exchanges mechanisms in order to evaluate how did soil methanol emissions evolve along the maize growing season. At the end of this poster, the hypothesis of decreasing soil methanol emissions along the maize growing season is proposed. [less ▲]

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See detailDes mini-jeux pour apprendre - Retour d'expérience dans un cours de chimie à l'université
le Maire, Nathalie ULg; Colaux, Catherine ULg; Fauconnier, Marie-Laure ULg et al

Conference (2016, June)

Dans le champ éducatif, les jeux ont régulièrement fait l’objet de réflexions et d’expérimentations visant à en exploiter leur potentiel au service de la compréhension, la motivation, la créativité ... [more ▼]

Dans le champ éducatif, les jeux ont régulièrement fait l’objet de réflexions et d’expérimentations visant à en exploiter leur potentiel au service de la compréhension, la motivation, la créativité, l’esprit critique etc. des étudiants. De la tortue LOGO aux jeux de simulation, l’exploitation du jeu dans l’enseignement a revêtu de multiples formes. Récemment la notion de gamification a enrichi cette ligne de recherche sur le jeu. Le concept de « gamification » désigne l’application d’éléments, de mécanismes, de processus ludiques à des situations, des contextes, des visées non ludiques. En la matière, Candy Crush, Farm Ville, Clash of Clans, etc. sont autant de jeux commerciaux qui remportent un grand succès aujourd’hui et peuvent fournir une source d’inspiration pour la création de mini-jeux pédagogiques. La présente communication relate la mise en place de « World of Chemistry », un parcours ludique mis en place de septembre à décembre 2015 en complément des supports de cours traditionnels. Ce parcours était composé de quatre mini-jeux portant sur des savoirs et des compétences identifiés comme difficiles en chimie et conçus explicitement pour incorporer des éléments de gamification issus de mini-jeux commerciaux variés. Cette expérimentation avait pour objectif d’étudier dans quelle mesure et à quelles conditions ces mini-jeux étaient susceptibles d’augmenter la fréquentation de la matière de chimie par les étudiants. A la lumière de l’usage fait des mini-jeux par 159 étudiants de première année d’université et de questionnaires de feedback qui leur ont été adressés, la communication évaluera la valeur de cette initiative pédagogique. [less ▲]

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See detailTaupins: à la découverte des odeurs de racines
Barsics, Fanny ULg; Delory, Benjamin; Fauconnier, Marie-Laure ULg et al

in Phytoma : La Défense des Végétaux (2016), 694

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