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See detailGrowth performance and meat quality of stress negative Piétrain pigs in the tropics: the case of Vietnam
Ha Xuan, Bo; Do Duc, Luc ULg; Moula, Nassim ULg et al

Poster (2013, October 11)

This study was carried out at the animal farm of Hanoi University of Agriculture, Vietnam to evaluate the growth performance and meat quality of stress negative Piétrain pigs. The study concerns the body ... [more ▼]

This study was carried out at the animal farm of Hanoi University of Agriculture, Vietnam to evaluate the growth performance and meat quality of stress negative Piétrain pigs. The study concerns the body weight (BW) of 116 pigs at 7.5 months of age and the ultrasound measurements on 83 live animals in order to determine backfat thickness, longissimus muscle depth and lean meat percentage (LM). Meat color (C.I.E L*, a* and b*), peak shear force (SF), pH at 24h (pH24) and at 45 minutes (pH45) post mortem were measured on 31 meat samples. Meat chemical composition (dry matter, protein, lipids and ash) was determined on 23 meat samples. All the data were analyzed according to a linear fixed model including gender effect (female and intact males) and Halothane genotype (CC and CT). The interaction between gender and genotype was not observed and therefore it was ignored from the final model. The results show that BW and LM were not significantly different between genotypes (P>0.21) while females were heavier than males (101.11 vs. 93.85 kg, P=0.0011). LM of female and male were respectively 63.27 and 63.56%. Gender and genotype didn’t affect meat quality and chemical composition (P>0.05) except pH24 (P=0.0011), a* (P<0.0001) and lipids (P=0.0012) for gender effect. The pH45 (6.50 and 6.45 for females and males) and pH24 (5.34 and 5.49 for females and males) were in the range corresponding to a normal meat. The obtained results show that stress negative Piétrain pigs have high lean meat percentage and satisfactory meat quality under tropical climatic conditions in Vietnam. [less ▲]

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See detailVitrification of immature equine oocytes
Gatez, Carine ULg; Ectors, Fabien ULg; Farnir, Frédéric ULg et al

Poster (2013, October 11)

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See detailCaractérisation de la race ovine Tazegzawth en Algerie: description morpho-biométrique et détermination d’une formule barymétrique
Moula, Nassim ULg; Philippe, François-Xavier ULg; Do Duc, Luc ULg et al

Poster (2013, October 11)

Le mouton Azegzaw est de robe blanche avec des taches noires à reflets bleuâtres sur le chanfrein, le pourtour des yeux et le lobe des oreilles. Non répertoriée par le standard des races ovines ... [more ▼]

Le mouton Azegzaw est de robe blanche avec des taches noires à reflets bleuâtres sur le chanfrein, le pourtour des yeux et le lobe des oreilles. Non répertoriée par le standard des races ovines algériennes, cette race présente pourtant des qualités intéressantes d'adaptation aux régions montagneuses de Kabylie, ce qui justifie la mise en œuvre d’un programme de conservation de ce patrimoine génétique original. L’objectif de cette étude, est (1) de caractériser la morpho-biométrie de moutons de race Tazegzawth et (2) de déterminer des équations barymétriques destinées à l’estimation du poids vif (PV) au départ des mensurations corporelles (périmètre thoracique: PT ; longueur du corps: LDC ; hauteur au garrot : HG ; hauteur à la croupe : HC). Au total, 89 sujets adultes (12 mâles, 77 femelles) sont concernés par cette étude. Les mensurations corporelles moyennes observées sont : - PV: 41,53±0,53 kg (mâle: 46,71kg et femelle: 40,72kg) ; - PT: 73,52±0,45 cm (mâle: 78,37cm et femelle: 72,77cm) ; - LDC: 89,10±0,44 cm (mâle: 93,82cm et femelle: 88,37cm) ; - HG: 71,40±0,43 cm (mâle: 76,06cm et femelle: 70,68cm); - HC: 70,14±0,42 (mâle: 74,80cm cm et femelle: 69,42). Les formules retenues pour l’estimation du PV sont: - Tous sexes confondus : PV=-0,025 HG2 + 4,78HG-169,88 (R2=0,87) - Femelles : PV = -0,038HG2 +6,58HG-232,39 (R2=0,84) - Mâles : PV = -0,16LDC2+0,21 HC2+284,92 (R2=0,99) En dépit de l’échantillon réduit dans l’étude, celui-ci a révélé des caractéristiques relativement homogènes. La détermination d’une formule barymétrique pourra venir en soutien d’une sélection incluant un objectif de croissance. [less ▲]

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See detailEvaluation of some production parameters in Kabyle local rabbit population
Moula, Nassim ULg; Michaux, Charles ULg; Do Duc, Luc ULg et al

Poster (2013, October 11)

In Algeria, the rabbit production is mainly based on local population to ensure a cheap supply of urban markets proteins. This sector may represent an important source of protein which is in large deficit ... [more ▼]

In Algeria, the rabbit production is mainly based on local population to ensure a cheap supply of urban markets proteins. This sector may represent an important source of protein which is in large deficit in the country. The rabbit production can be justified by its advantages such as short life cycle and high prolificacy. The objective of the present study is to evaluate production performances of Kabyle local rabbit population. Litter size, growth rate, body slaughter weight, feed conversion ratio and mortality rate are considered. The experiment was carried out on 30 animals (6 males and 24 females) for reproduction in the Ath Waghlis region (north-east Algeria). Rabbits have been raised in pens with 1 male and 4 females per pen. The commercial feed was supplied ad libitum to animals. Results reported shown that total number of rabbits born, number born alive, number to weaned, slaughter weight at 84 days of age, feed conversion ratio (from weaning to slaughter) and survival rate (from birth to slaughter) were 7.1, 6.1, 5.3 rabbits, 1.90kg, 3.16 and 73% respectively. The performances of the Kabyle breed could be improved by purebred selection as well as by crossing with exotic breeds. [less ▲]

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See detailIMMUNOHISTOCHEMICAL ANALYSIS OF TRANSFORMING GROWTH FACTOR BETA 1 AND ITS SIGNALLING PATHWAYS IN CANINE IDIOPATHIC PULMONARY FIBROSIS
Krafft, Emilie ULg; Laurila, HP; Rajamäki, MM et al

in Proceedings of the 23th ECVIM Meeting (2013, September)

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See detailIs 5-Hydroxytryptamine (serotonin) involved in the pathogenesis of idiopathic pulmonary fibrosis in dogs ?
Krafft, Emilie ULg; Roels, Elodie ULg; Laurila et al

in Proceedings of the 23th ECVIM Meeting (2013, September)

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See detailComparison of four cartridge type automated blood gas analysers for arterial blood gas analysis in dogs
Roels, Elodie ULg; Delvaux, François ULg; Farnir, Frédéric ULg et al

in Proceedings of the 23th ECVIM Meeting (2013, September)

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See detailEstimation of genetic parameters of racing traits of Arabian horses in Algeria
Tennah, Safia ULg; Kafidi, Nacereddine; Antoine-Moussiaux, Nicolas ULg et al

Poster (2013, August)

The results of the flat races organized in Algeria from 1995 to 2007 by the Algerian Horse Racing Society, were used to estimate genetic parameters of racing performances of Arabian horses. The data ... [more ▼]

The results of the flat races organized in Algeria from 1995 to 2007 by the Algerian Horse Racing Society, were used to estimate genetic parameters of racing performances of Arabian horses. The data consist of 36 492 race records, obtained from 913 horses. The pedigree file of the horses includes 1812 animals from 166 stallions and 392 mares. The analysis was performed on two traits: the logarithm of average annual virtual earnings per start (LAEV/S) and a normalized ranking (PERF). To identify the fixed effects to be included in the genetic model, a preliminary analysis was conducted using the General Linear Models (GLM) procedure from SAS software. The effects of age (3 to 8 years and older), sex (male or female), year (1995 to 2007) and the interaction between year of the race and age and between sex and age were included in the model for both traits. In addition, two random effects, a direct genetic effect of the animal and a permanent environmental effect were included in the mixed model. The variance components and genetic parameters were estimated by the restricted maximum likelihood (REML), procedure using the MTDFREML program. The analysis, using a repeatable animal model, led to the following estimation of genetic parameters: for LAEV/S, heritability was 0.23 (±0.04), while estimate of repeatability was 0.34 (±0.04). The heritability for the normalized ranking was higher, 0.37 (±0.05), indicating that this trait might be more appropriate for breeding programs of Arabian horses in Algeria. The repeatability estimate for the normalized ranking was 0.59 (±0.04) and the genetic correlation between this trait and LAEV/S was 0.79. [less ▲]

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See detailTransforming growth factor beta 1 and its activation, storage and signalling pathways in canine idiopathic pulmonary fibrosis.
Krafft, Emilie ULg; Heikkilä, H.P.; Rajamäki, M.M. et al

Scientific conference (2013, July)

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See detailUse of ancestral haplotypes in Genome Wide Association Studies
Druet, Tom ULg; Farnir, Frédéric ULg

in Gondro, Cedric; van der Werf, Julius; Hayes, Ben (Eds.) Genome-Wide Association Studies and Genomic Prediction (2013)

We herein present a haplotype-based method to perform genome-wide association studies. The method relies on hidden Markov models to describe haplotypes from a population as a mosaic of a set of ancestral ... [more ▼]

We herein present a haplotype-based method to perform genome-wide association studies. The method relies on hidden Markov models to describe haplotypes from a population as a mosaic of a set of ancestral haplotypes. For a given position in the genome, haplotypes deriving from the same ancestral haplotype are also likely to carry the same risk alleles. Therefore, the model can be used in several applications such as haplotype reconstruction, imputation, association studies or genomic predictions. We illustrate then the model with two applications: the fine-mapping of a QTL affecting live weight in cattle and association studies in a stratified cattle population. Both applications show the potential of the method and the high linkage disequilibrium between ancestral haplotypes and causative variants. [less ▲]

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See detailEgg Production Performance of the Local Kabyle Hen and its Crossbreeds with ISA-Brown Strain in Semi-Intensive Conditions
Moula, Nassim ULg; Mamadou Tandiang, Diaw; Salhi, Abdellah et al

in International Journal of Poultry Sciences (2013), 12(3), 148-152

A cross between the ISA-Brown industrial strain and an Algerian local (Kabyle) breed was compared with the Algerian local (Kabyle) hen. The comparison during the egg production period was done in ... [more ▼]

A cross between the ISA-Brown industrial strain and an Algerian local (Kabyle) breed was compared with the Algerian local (Kabyle) hen. The comparison during the egg production period was done in individual cages, in a ventilated henhouse without any particular isolation. The same commercial diet has been provided over three time periods (35 to 36 weeks, 50 to 51 weeks and 65 to 66 weeks of age). Egg number and body weight at first egg was significantly lower in the Kabyle local breed. Mortality was almost equal during the breeding period and the egg production periods for the two genotypes. The Kabyle hen eggs, in spite of a weight which was lower (43.70 to 53.37 g according to the age), gave yolk to albumen ratio similar to the ISAKAB cross (0.52 to 0.58 according to age). Finally, the ISAKAB cross was better in terms of egg production and egg quality than the local hen, in test conditions. [less ▲]

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See detailMechanical testing of a new osteotomy design for tibial tuberosity advancement using the Modified Maquet Technique (MMT)
Brunel, L.; Etchepareborde, S.; Barthelemy, Nicolas ULg et al

in Veterinary and Comparative Orthopaedics and Traumatology (2013), 26(1), 47-53

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See detailHealth screening to identify opportunities to improve preventive medicine in cats and dogs:focus on nutrition status
Diez, Marianne ULg; Picavet, Philippe; Ricci, Rebecca et al

in Hesta, Myriam (Ed.) Proceedings of the 17 th European Society of Veterinary and Comparative Nutrition Congress (2013)

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See detailRelationship between echocardiographic measurements and body size in horses.
Al Haidar, Ali; Leroux, Aurélia ULg; Borde, Laura ULg et al

in Journal of Equine Veterinary Science (2013), 33(2), 107-114

The objectives of this work were to evaluate the optimal body size variable, and to determine mathematical model that best fits echocardiographic measurements to body size in the equine species. 150 ... [more ▼]

The objectives of this work were to evaluate the optimal body size variable, and to determine mathematical model that best fits echocardiographic measurements to body size in the equine species. 150 horses of various breeds, aged 1 week to 17 years old, body weight (BW) 38 to 890 kg, and free from cardiac disease were used in this study. Based on their age (i.e. younger or older than 2 years), animals were separated into 2 groups. In each horse, the body weight (BW), height at withers (HT), thoracic circumference (TC) and the body length (BL) were measured, the body surface area (BSA) was calculated, and a standard two-dimensional and M-mode echocardiography was performed allowing classic cardiac measurements to be taken. Correlations between each echocardiographic measurement and each of the anthropometric variables were assessed via a Pearson’s product-moment analysis and using linear, logarithmic, and power regression analysis in all animals and within the 2 groups. The dimensional, but not the functional, echocardiographic measurements showed a close correlation with all body size variables, especially during growth. The relationship between cardiac measurements and body size was best described using power regression models with TC as the anthropometric variable, but the relationship was also very accurately described using BW, BSA, or BL. In the equine species, dimensional echocardiographic reference values should be established using power regression equations on TC. Such a correction for body size could increase the diagnostic value of echocardiography in equine cardiology. [less ▲]

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See detailExperimental model of equine alveolar macrophage stimulation with TLR ligands.
Waldschmidt, Ingrid; Pirottin, Dimitri ULg; Art, Tatiana ULg et al

in Veterinary Immunology and Immunopathology (2013), 155(1-2), 30-37

Pulmonary diseases are common in horses and have a major economic impact on the equine industry. Some of them could be associated with an inadequate immune response in the lung, but methods to evaluate ... [more ▼]

Pulmonary diseases are common in horses and have a major economic impact on the equine industry. Some of them could be associated with an inadequate immune response in the lung, but methods to evaluate this response in horses are lacking. The aim of this study was to develop and validate an experimental model that could be applied in several physiological and pathological conditions to assess the innate immune response of equine pulmonary cells. Equine alveolar macrophages (AMs) obtained from bronchoalveolar lavages were isolated from other cells by adhesion. TLR2, 3, and 4 expression in AMs was studied and their responses to commercial ligands (respectively FSL-1, Poly(I:C), and LPS) were evaluated after determination of the appropriate dose and time of incubation. TLR responses were assessed by measuring cytokine production using (1) gene expression of TNFalpha, IFNbeta, Il-1beta, and IFNalpha by qPCR (indirect method); and (2) cytokine production for TNFalpha and IFNbeta by ELISA (direct method). TLR 2, 3, and 4 were expressed by AMs. TLR 2 stimulation with 10ng/mL of FSL-1 during 3h significantly increased IL-1beta and TNFalpha gene expression. TLR 3 stimulation with 1000ng/mL of Poly(I:C) during 1h increased IFNbeta, IFNalpha, Il-1beta and TNFalpha expression. TLR 4 stimulation with 100ng/mL of LPS during 3h increased TNFalpha, IFNbeta, and Il-1beta expression. Results obtained by ELISA quantification of TNFalpha and IFNbeta produced by AMs following stimulation during 6h were similar: FSL-1 increased TNFalpha production but not IFNbeta, Poly(I:C) and LPS increased production of IFNbeta and TNFalpha. In conclusion, pulmonary innate immunity of horses can be assessed ex vivo by measuring cytokine production following stimulation of AMs with TLR agonists. This experimental model could be applied under several conditions especially to improve the understanding of equine respiratory disease pathogenesis, and to suggest novel therapeutic opportunities. [less ▲]

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See detailGrowth performance and sperm quality of stress negative Piétrain boars and their hybrids with Duroc
Do Duc, Luc ULg; Ha Xuan, Bo; Farnir, Frédéric ULg et al

in Journal of Science and Development (2013), 11(2),

This study was conducted to evaluate the effects of genetic background and season on growth performance and semen quality of boars. Five genetic groups were evaluated, including stress-negative Piétrain ... [more ▼]

This study was conducted to evaluate the effects of genetic background and season on growth performance and semen quality of boars. Five genetic groups were evaluated, including stress-negative Piétrain boars with CC (PiCC) or CT (PiCT) halothane genotypes, and Piétrain x Duroc hybrid boars with various compositions (25% (PiDu25), 50% (PiDu50) or 75% (PiDu75) of Piétrain origin). The results showed that genetic group has a significant effect on growth performance and semen quality. The hybrid boars PiDu25, PiDu50 and PiDu75 had better growth rate, but lower lean <br />meat in comparison with pure stress-negative Piétrain, except PiDu75 boars. PiDu25, PiDu50 and PiCC boars demonstrated high semen quality. A season effect was observed on most of semen quality traits of pure stress-negative Piétrain as well as hybrid boars with different genetic constitution. Sperm concentration was lower in Summer and Autumn, higher in Winter and Spring. The Piétrain x Duroc hybrid boars, especially PiDu75 seem to be promising as terminal boars. [less ▲]

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See detailUtilization of Large White x Mong Cai crossbred sows, Duroc and stress negative Piétrain boars for the production of fattening pigs under household conditions in northern Vietnam
Do Duc, Luc ULg; Clinquart, Antoine ULg; Vu Dinh, Ton et al

in Animal Production Science (2013)

The objective of the present study was to compare Duroc (DU) and stress-negative Piétrain (PI) as terminal sires mated to F1(Large White x Mong Cai) sows (F1) on productivity, carcass characteristics ... [more ▼]

The objective of the present study was to compare Duroc (DU) and stress-negative Piétrain (PI) as terminal sires mated to F1(Large White x Mong Cai) sows (F1) on productivity, carcass characteristics, meat quality and meat chemical composition under household conditions in Hai Duong province, Vietnam. The reproduction performances were based on 74 litters of 42 F1 sows from 12 households and the semen from 11 Duroc and 10 Piétrain boars. Data on production and carcass traits were collected from 76 DUxF1and 67 PIxF1 crossbred fattening pigs. Longissimus dorsi samples from 55 and 37 of DUxF1 and PIxF1 respectively were analyzed to determine meat quality and meat chemical composition. The results show that household affected production, carcass, and meat quality traits (P<0.05) as well as reproduction (weights at birth and weaning, P<0.01). Inversely, boar breeds did not influence (P>0.06) on reproduction traits (except individual weight of born alive pigs and weaned pigs, P<0.01), production traits, meat chemical composition and meat quality (except pH at 4 days post mortem, P<0.05) while lean meat percentage of Piétrain-sired pigs was higher than those of Duroc-sired pigs (P<0.05). The utilization of Piétrain boars does not affect reproduction performances, growth rate and meat quality while improving lean meat percentage in comparison to Duroc boars. This suggests that the use of stress-negative Piétrain boars as terminal sires would improve lean meat percentage under household conditions in North Vietnam. [less ▲]

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See detailEffect of breed, sex, age and body weight on Echocardiographic Measurements in the Equine Species
Al Haidar, Ali; Farnir, Frédéric ULg; Deleuze, Stefan ULg et al

in Research in Veterinary Science (2013), In press

Little is known about the effect of various animal's signalment variables on echocardiographic reference values in the equine species. This study was performed to evaluate the effect of sex, breed, age ... [more ▼]

Little is known about the effect of various animal's signalment variables on echocardiographic reference values in the equine species. This study was performed to evaluate the effect of sex, breed, age and body weight (BW) on echocardiographic measurements in the equine species. Echocardiography was performed on 212 ponies or horses of various breeds, aged from 1 day to 37 years old (mean ± SD: 7.8 ± 5.8 years), BW 38-890 kg (mean ± SD: 421 ± 133 Kg), and free of cardiac disease. Fifty of those animals aged from 2 months to 35 years old (mean ± SD: 11.6 ± 6.4 years old); BW 77-662 kg (mean ± SD: 436 ± 135 kg) were also examined using the pulsed-wave Doppler mode. Standard two-dimensional and M-mode echocardiography were performed on all animals. Standard pulsed-wave Doppler examination of each cardiac valve was performed on the 50 first examined animals. Data were analysed using a general linear model including the effect of sex, age, breed and BW after logarithmic transformation of the data. Therefore, the same analysis was performed separately on animals aged  2 years-old and on older animals. All dimensional echocardiographic measurements were significantly affected by BW and most of them were significantly affected by breed, but not by sex. Only the aortic and the pulmonary artery internal diameter were significantly affected by age. None of the Doppler measurements were significantly affected by the tested variables. In conclusion, in the equine species, dimensional echocardiographic reference values should be established using regression equations as a function of BW, which could increase the diagnostic value of this leading technique in equine cardiology. Breed could also have an effect on those measurements. [less ▲]

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See detailReproductive and productive performances of the stress-negative Piétrain pigs in the tropics: the case of Vietnam
Do Duc, Luc ULg; Ha Xuan, Bo; Thomson, Peter et al

in Animal Production Science (2013), 53(2), 173-179

The objective of the present study is to investigate the reproduction, production and semen traits of the stressnegative Piétrain line of pigs in the north of Vietnam, as well as to identify various ... [more ▼]

The objective of the present study is to investigate the reproduction, production and semen traits of the stressnegative Piétrain line of pigs in the north of Vietnam, as well as to identify various factors (halothane genotype, generationorigin, parity, sex and month of the year) acting on these traits. The experiment was carried out on a purebred nucleus on Dong Hiep farm, Haiphong province in Vietnam. The reproduction performances and the sperm quality measures were based on 12 sows and 5 boars imported from Belgium as well as on 29 sows and 14 boars born in Vietnam, while the production performances were recorded only from 251 animals born in Vietnam. No significant differences were observed between halothane genotypes of sows for reproduction traits (P>0.0578). At the age of 7.5 months, bodyweight, backfat thickness and longissimus depth (7.5) of homozygous-dominant pigs were higher (P<0.05) than in heterozygotes. Homozygous-dominant boars had higher total number of spermatozoa than heterozygote boars (P < 0.05); similarly, Vietnam boars also presented higher total number of spermatozoa values than Belgium boars (P < 0.001). Vietnam sows had longer duration of pregnancy, more piglets born alive and heavier litter weight of weaning than Belgium sows (P < 0.01). Bodyweight at birth, at weaning, at 2, 5.5 and 7.5 months of age increased from the first to third parity (P < 0.05). Gilts had more lean meat than intact boars (P < 0.01). Compared with observations made in temperate countries, the reproduction and production performances, sperm concentration and total number of spermatozoa of stress-negative Piétrain pigs were reduced, whereas lean meat percentage was not affected. This might be due to the different climate, although improving values for animals born in Vietnam might indicate adaptation of the animals and/or of the technicians with that breed. The results support the interest of the use of this new Piétrain line in tropical countries with ecological and climatic conditions similar to Vietnam. [less ▲]

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