Assessing interventions by quantitative risk assessment tools to reduce the risk of human salmonellosis from fresh pork meat in Belgium.
Delhalle, Laurent ; Saegerman, Claude ; Farnir, Frédéric et al
in Journal of Food Protection (2009), 72Detailed reference viewed: 85 (32 ULg)
Evaluation quantitative de risque concernant Salmonella dans la filière de production de viande de porc en Belgique
Delhalle, Laurent ; Saegerman, Claude ; Farnir, Frédéric et al
Conference (2008, September)Detailed reference viewed: 27 (1 ULg)
Marker assisted selection of bovine for improved milk composition.
; ; et al
The present invention provides a method of genotyping bovine for improved milk production traits by determining the GHR genotypic state of said bovine, wherein the GHR gene and polymorphisms within said ... [more ▼]
The present invention provides a method of genotyping bovine for improved milk production traits by determining the GHR genotypic state of said bovine, wherein the GHR gene and polymorphisms within said gene have been found to be associated with such improved milk production traits. [less ▲]Detailed reference viewed: 30 (5 ULg)
Duration of anaesthetic effect and effects of multiple doses and concentrations of tetracaine hydrochloride eyedrops on the normal equine cornea
Monclin, Sébastien ; Farnir, Frédéric ; Grauwels, Magda
Poster (2008, May)Detailed reference viewed: 17 (0 ULg)
Pathogenicity and thermotolerance of entomopathogenic fungi for the control of the scab mite, Psoroptes ovis.
Lekimme, Mireille ; Focant, Charles ; Farnir, Frédéric et al
in Experimental & Applied Acarology (2008), 46(1-4), 95-104
Psoroptes ovis is responsible for a highly contagious skin condition, both in sheep and cattle. This parasite has a marked economical impact in the sheep and cattle industry. Biological control is ... [more ▼]
Psoroptes ovis is responsible for a highly contagious skin condition, both in sheep and cattle. This parasite has a marked economical impact in the sheep and cattle industry. Biological control is considered as a realistic alternative to chemotherapeutic control. Laboratory experiments were carried out to evaluate the pathogenicity and the thermotolerance of twelve isolates of entomopathogenic fungi from four genera (Beauveria Vuillemin, Metarhizium Sorokin, Paecilomyces Bainier and Verticillium Nees). The pathogenicity was evaluated by the survival of P. ovis females after exposure to 10(6) to 10(8) conidia ml(-1) in humidity chambers. Results revealed intra- and interspecies differences. All isolates with the exception of B. bassiana IHEM3558 and V. lecanii MUCL8672 induced 50% mortality within 2 days at the highest concentration. At this concentration the entire mite population became infected with all isolates but B. bassiana IHEM3558; however, only four isolates gave rise to 100% infected cadavers at the lowest concentration. The thermotolerance of each isolate was evaluated by measuring its growth on an artificial medium kept between 25 and 37.5 degrees C. All isolates were able to grow up to 30 degrees C but only two, M. anisopliae IHEM18027 and Paecilomyces farinosus MUCL18885, tolerated temperatures up to 35 degrees C. These two isolates could be considered as good candidates for further use as biopesticide taking into account their virulence and thermotolerance. Other critical factors linked with the implementation of this type of biocontrol in P. ovis infected animals are discussed. [less ▲]Detailed reference viewed: 64 (4 ULg)
Malignant catarrhal fever induced by alcelaphine herpesvirus 1 is associated with proliferation of CD8+ T cells supporting a latent infection.
Dewals, Benjamin G ; Boudry, Christel ; Farnir, Frédéric et al
in PLoS ONE (2008), 3(2), 1627
Alcelaphine herpesvirus 1 (AlHV-1), carried by wildebeest asymptomatically, causes malignant catarrhal fever (WD-MCF) when cross-species transmitted to a variety of susceptible species of the Artiodactyla ... [more ▼]
Alcelaphine herpesvirus 1 (AlHV-1), carried by wildebeest asymptomatically, causes malignant catarrhal fever (WD-MCF) when cross-species transmitted to a variety of susceptible species of the Artiodactyla order. Experimentally, WD-MCF can be induced in rabbits. The lesions observed are very similar to those described in natural host species. Here, we used the rabbit model and in vivo 5-Bromo-29-Deoxyuridine (BrdU) incorporation to study WD-MCF pathogenesis. The results obtained can be summarized as follows. (i) AlHV-1 infection induces CD8+ T cell proliferation detectable as early as 15 days postinoculation. (ii) While the viral load in peripheral blood mononuclear cells remains below the detection level during most of the incubation period, it increases drastically few days before death. At that time, at least 10% of CD8+ cells carry the viral genome; while CD11b+, IgM+ and CD4+ cells do not. (iii) RT-PCR analyses of mononuclear cells isolated from the spleen and the popliteal lymph node of infected rabbits revealed no expression of ORF25 and ORF9, low or no expression of ORF50, and high or no expression of ORF73. Based on these data, we propose a new model for the pathogenesis of WD-MCF. This model relies on proliferation of infected CD8+ cells supporting a predominantly latent infection. [less ▲]Detailed reference viewed: 90 (39 ULg)
Kết quả bước đầu đánh giá khả năng sinh trưởng của lợn Pietrain kháng stress nuôi tại Hải Phòng (Việt Nam)
Do Duc, Luc ; ; et al
in Journal of Agricultural Science and Developement - Hanoi University of Agriculture, Vietnam (2008), 6(6), 549-555
The present study was carried out on 19 stress negative Piétrain pigs (Pietrain ReHal), consisting of 13 gilts and 6 young boars imported from Belgium, raised in the livestock farm of Dong Hiep (Hai Phong ... [more ▼]
The present study was carried out on 19 stress negative Piétrain pigs (Pietrain ReHal), consisting of 13 gilts and 6 young boars imported from Belgium, raised in the livestock farm of Dong Hiep (Hai Phong) in order to evaluate growth performances and their adaptability in the North of Vietnam. Results showed that the average body weight of the whole herd at 2, 4, 5.5, and 8.5 months old was 19.05, 51.05, 85.82, and 119.47 kg, respectively. During the growing periods, except the first stage, the male grew faster than the female and the pigs of the CT genotype grew faster than those of CC genotype although the difference was not significant (P>0.05). The average daily gain (ADG) was 528.56 grams for the whole herd. The ADG was higher for the male (546.48 grams) than for the female (520.29 grams), and its was higher for the CT than the CC, but the difference was not statistically significant (P>0.05). The feed conversion ratio (FCR) was 2.69 kg. The estimated lean percentage at 8.5 months old was 64.08%. The results indicate that Piétrain stress negative pigs could develop well on the farm conditions in Hai Phong, Vietnam. [less ▲]Detailed reference viewed: 188 (8 ULg)
Morphological echocardiographic measurements: reference values as a function of body size in equids
; Sandersen, Charlotte ; et al
Poster (2008)Detailed reference viewed: 16 (1 ULg)
Equine third metacarpal bone properties as evaluated by axial transmission quantitative ultrasound and quantitative computed tomography
; Farnir, Frédéric ; et al
Poster (2008)Detailed reference viewed: 30 (6 ULg)
Large-scale haplotype reconstruction using pedigree information and a Hidden Markov Model
Druet, Tom ; Coppieters, Wouter ; Farnir, Frédéric et al
Poster (2008)Detailed reference viewed: 19 (0 ULg)
L'évaluation quantitative du risque microbiologique: revue de trois modèles liés à Salmonella dans les aliments.
Delhalle, Laurent ; Saegerman, Claude ; Farnir, Frédéric et al
in Annales de Médecine Vétérinaire (2008), 152
Following international agreements and european legislation, risk analysis has become a systematic approach to control the food chain safety. The microbial risks in the food chain and their consequences ... [more ▼]
Following international agreements and european legislation, risk analysis has become a systematic approach to control the food chain safety. The microbial risks in the food chain and their consequences for public health can be assessed and managed more effectively. This paper gives a general description of the risk analysis principles based on the Codex Alimentarius commission. This work highlights the advantages and disadvantages of risk analysis and also treats the problems to achieve a complete “farm to fork” model. Salmonella in different food types is taken as an example to illustrate quantitative risk assessment. Two models concerning eggs and broiler chickens developed by the World Health Organization/Food and Agriculture Organization consortium and a third model concerning pigs developed by the Veterinary Laboratories Agency of the United Kingdom are described. An analysis of the methods used for the construction of these two models is also presented. The interest of these three models is that they also include risk management options to limit the spread of food-borne infections. They also describe the final risk for a population following consumption of foodstuffs. These models are valuable tools for health officials but also for feed industry. [less ▲]Detailed reference viewed: 257 (33 ULg)
Risk factors for Salmonella and hygiene indicators in the 10 largest Belgian pig slaughterhouses.
Delhalle, Laurent ; ; et al
in Journal of Food Protection (2008), 77
A survey was conducted to collect data on Salmonella prevalence, Escherichia coli counts (ECCs), and aerobic bacteria colony counts (ACCs) on pig carcasses after chilling at the 10 largest Belgian pig ... [more ▼]
A survey was conducted to collect data on Salmonella prevalence, Escherichia coli counts (ECCs), and aerobic bacteria colony counts (ACCs) on pig carcasses after chilling at the 10 largest Belgian pig slaughterhouses during 2000 through 2004. Potential risk factors of contamination associated with production parameters, technical descriptions of the installations, and cleaning and disinfection methods were assessed during investigations in the slaughterhouses. These variables were used first in a univariate analysis and then were extended to a multivariate analysis with a logistic mixed regression model for Salmonella and a linear mixed model for ECCs and ACCs with slaughterhouses as the random effect. The results indicated high variability concerning Salmonella contamination among the 10 slaughterhouses, with prevalence ranging from 2.6 to 34.3% according to the area of origin. The median ECC and median ACC ranged from 0.43 to 1.11 log CFU/cm2 and from 2.37 to 3.65 log CFU/cm2, respectively. The results of the logistic and linear regressions revealed that some working practices such as scalding with steam, second flaming after polishing, and complete cleaning and disinfection of the splitting machine several times a day were beneficial for reducing Salmonella prevalence, ECCs, and ACCs. Changing the carcass hooks just before chilling, using water as the cleaning method, and a higher frequency of disinfection of the lairage seemed to be protective against E. coli in the multivariate mixed linear model. The monitoring of critical points, slaughterhouse equipment, good slaughtering practices, and effective washing and disinfection are the keys to obtaining good microbiological results. [less ▲]Detailed reference viewed: 310 (14 ULg)
Modifications urodynamiques et morphologiques sur les portions basses du tractus urogénital après administration d'oestriol seul ou en combinaison avec la PPA chez des chiennes entières et stérilisées
Hamaide, Annick ; ; Farnir, Frédéric et al
in Proceedings du congrès AFVAC (2007, December)Detailed reference viewed: 12 (1 ULg)
Développement d’un outil pour la modélisation de la contamination de la viande de porc à l’abattoir par Salmonella.
Delhalle, Laurent ; Farnir, Frédéric ; Daube, Georges
Poster (2007, June)Detailed reference viewed: 18 (3 ULg)
Exploring the risk factors for Salmonella in the ten biggest Belgian pig slaughterhouses.
Delhalle, Laurent ; ; et al
Poster (2007, May)Detailed reference viewed: 15 (0 ULg)
The TNF/ADAM 17 system: implication of an ADAM 17 haplotype in the clinical response to infliximab in Crohn's disease
Dideberg, Vinciane ; Theatre, Emilie ; Farnir, Frédéric et al
in European Journal of Clinical Investigation (2007, May), 37(Suppl. 1), 79Detailed reference viewed: 46 (5 ULg)
Novel Crohn disease locus identified by genome-wide association maps to a gene desert on 5p13.1 and modulates expression of PTGER4.
Libioulle, Cécile ; Louis, Edouard ; Hansoul, Sarah et al
in PLoS Genetics (2007), 3(4), 538-543
To identify novel susceptibility loci for Crohn disease (CD), we undertook a genome-wide association study with more than 300,000 SNPs characterized in 547 patients and 928 controls. We found three ... [more ▼]
To identify novel susceptibility loci for Crohn disease (CD), we undertook a genome-wide association study with more than 300,000 SNPs characterized in 547 patients and 928 controls. We found three chromosome regions that provided evidence of disease association with p-values between 10(-6) and 10(-9). Two of these (IL23R on Chromosome 1 and CARD15 on Chromosome 16) correspond to genes previously reported to be associated with CD. In addition, a 250-kb region of Chromosome 5p13.1 was found to contain multiple markers with strongly suggestive evidence of disease association (including four markers with p < 10(-7)). We replicated the results for 5p13.1 by studying 1,266 additional CD patients, 559 additional controls, and 428 trios. Significant evidence of association (p < 4 x 10(-4)) was found in case/control comparisons with the replication data, while associated alleles were over-transmitted to affected offspring (p < 0.05), thus confirming that the 5p13.1 locus contributes to CD susceptibility. The CD-associated 250-kb region was saturated with 111 SNP markers. Haplotype analysis supports a complex locus architecture with multiple variants contributing to disease susceptibility. The novel 5p13.1 CD locus is contained within a 1.25-Mb gene desert. We present evidence that disease-associated alleles correlate with quantitative expression levels of the prostaglandin receptor EP4, PTGER4, the gene that resides closest to the associated region. Our results identify a major new susceptibility locus for CD, and suggest that genetic variants associated with disease risk at this locus could modulate cis-acting regulatory elements of PTGER4. [less ▲]Detailed reference viewed: 104 (11 ULg)
Biochemical bone markers in Ardenner horses: comparison between sound horses and horses with juvenile form of interphalangeal joint disease
; Lejeune, Jean-Philippe ; et al
in Hippos 2007 (2007)Detailed reference viewed: 31 (0 ULg)
Morphometric study of interphalangeal joints in Ardenner horses with juvenile osteoarthropathy
Gabriel, Annick ; Spelmans, Céline ; Farnir, Frédéric et al
in Slovenian Veterinary Research (2007), 44Detailed reference viewed: 51 (7 ULg)
Evaluation of serum osteocalcin and CTX-I in Ardenner horses with special reference to juvenile interphalangeal joint disease.
; ; Lejeune, Jean-Luc et al
in Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine (2007), 54(9), 458-63
The first aim of this study was to establish a profile of age-related normal serum concentrations of osteocalcin (OC) in Ardenner horses. For this first part, blood samples from 49 healthy Ardenner horses ... [more ▼]
The first aim of this study was to establish a profile of age-related normal serum concentrations of osteocalcin (OC) in Ardenner horses. For this first part, blood samples from 49 healthy Ardenner horses were collected. The second aim was to study two biochemical markers of bone metabolism, OC and a carboxy-terminal cross-linking telopeptide of type I collagen (CTX-I), in 30 young Ardenner horses during 1 year. Amongst them, 17 showed lesions of juvenile degenerative joint disease in the distal forelimbs. A specific radioimmunoassay for equine OC was used to measure the serum concentration of the marker. The serum concentration of CTX-I was measured using a commercially available human assay validated for use in the horse. The effect of age, sex, season and health status (with or without lesions) was assessed. Levels of OC fall between birth and the adult stage: this decrease being most marked between birth and 1 year of age. This age-related decrease of OC was confirmed in the 30 young Ardenner horses, but CTX-I levels remained constant in this group. The Levels of the two markers changed significantly with the season with higher concentrations during the winter. No significant difference was shown either between the two sexes or between the two health statuses. [less ▲]Detailed reference viewed: 34 (11 ULg)