References of "Farnir, Frédéric"
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See detailLinkage disequilibrium on the bovine X chromosome: Characterization and use in quantitative trait locus mapping
Sandor, Cynthia ULg; Farnir, Frédéric ULg; Hansoul, Sarah ULg et al

in Genetics (2006), 173(3), 1777-1786

We herein demonstrate that in the Holstein-Friesian dairy cattle population, microsatellites are as polymorphic on the X chromosome as on the autosomes but that the level of linkage disequilibrium between ... [more ▼]

We herein demonstrate that in the Holstein-Friesian dairy cattle population, microsatellites are as polymorphic on the X chromosome as on the autosomes but that the level of linkage disequilibrium between these markers is higher on the X chromosome than on the autosomes. The latter observation is not compatible with the small male-to-female ratio that prevails in this population and results in a higher gonosomal than autosomal effective population size. It suggests that the X chromosome undergoes distinct selective or mutational forces. We describe and characterize a novel Markovian approach to exploit this linkage disequilibrium to compute the probability that two chromosomes are identical-by-descent conditional on flanking marker data. We use the ensuing probabilities in a restricted maximum-likelihood approach to search for quantitative trait loci (QTL) affecting 48 traits of importance to the dairy industry and provide evidence for the presence of QTL affecting 5 of these traits on the bovine X chromosome. [less ▲]

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See detailEffects of a reduction of diet crude protein content on gaseous emissions from deep-litter pens for fattening pigs
Philippe, François-Xavier ULg; Laitat, Martine ULg; Canart, Bernard et al

in Animal Research (2006), 55(5), 397-407

Two successive batches of 32 fattening pigs per batch were each divided into 2 homogenous groups of 16 pigs fed either a high crude protein (CP) level diet (HP-groups) or a low crude protein level diet ... [more ▼]

Two successive batches of 32 fattening pigs per batch were each divided into 2 homogenous groups of 16 pigs fed either a high crude protein (CP) level diet (HP-groups) or a low crude protein level diet balanced with synthetic amino acids (LP-groups). Pigs were raised on straw-based deep litters in separate rooms according to diets. Once a month, the emissions of ammonia (NH3), nitrous oxide (N2O), methane (CH4), carbon dioxide (CO2) and water vapour (H2O) were measured continuously for 6 days consecutively. The mean nitrogen (N) intakes of pigs from HP-groups and LP-groups were 6.83 kg and 5.78 kg per pig respectively with mean initial and final pig body weights of 26.6 and 111.4 kg. There was no significant difference between the daily weight gains with regards to the diet CP content. At the end of the fattening periods, the N-contents of the litters were on average 1.84 kg per pig for the HP-groups and 1.56 kg per pig for the LP-groups. Gaseous emissions in the room with LP-groups were, compared with the emissions in the room with HP-groups, 26.1% lower for NH3 (10.60 vs. 14.35 g per pig per day), 12.8% lower for CH4 (13.12 vs. 15.04 g per pig per day) and 2 times higher for N2O (1.02 vs. 0.52 g per pig per day). The emissions of CO2 and H2O were not significantly different according to the diet CP level. [less ▲]

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See detailEvaluation of the urodynamic and hemodynamic effects of orally administered phenylpropanolamine and ephedrine in female dogs.
Carofiglio, Francesca; Hamaide, Annick ULg; Farnir, Frédéric ULg et al

in American Journal of Veterinary Research (2006), 67(4), 723-730

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See detailUrodynamic and morphologic changes in the lower portion of the urogenital tract after administration of estriol alone and in combination with phenylpropanolamine in sexually intact and spayed female dogs
Hamaide, Annick ULg; Grand, Jean-Guillaume; Farnir, Frédéric ULg et al

in American Journal of Veterinary Research (2006), 67(5), 901-908

OBJECTIVE: To compare the urodynamic and morphologic effects of the administration of estriol alone and in combination with phenylpropanolamine on the lower portion of the urogenital tract in female dogs ... [more ▼]

OBJECTIVE: To compare the urodynamic and morphologic effects of the administration of estriol alone and in combination with phenylpropanolamine on the lower portion of the urogenital tract in female dogs. ANIMALS: 3 sexually intact and 3 spayed female Beagles without urinary incontinence. PROCEDURE: Dogs received estriol (2 mg, PO) once daily for 7 days followed by estriol (2 mg, PO) and phenylpropanolamine (1.5 mg/kg, PO) once daily for 7 days. Urethral pressure profilometry, diuresis cystometry, and vaginourethrography were performed before treatment (day 0) and at days 7 and 14. The maximum urethral pressure (MUP) and closure pressure (MUCP), urethral functional and anatomic profile lengths, integrated pressure (IP), plateau, distance before MUP, maximum meatus pressure, threshold pressure, threshold volume, compliance, urethral length, and vaginal length and width were measured. RESULTS: Before treatment, no urodynamic differences were observed between the 2 groups; however, vaginal length and width were significantly shorter in spayed dogs. Compared with day 0 values, estriol treatment significantly increased MUP, MUCP, and IP values at day 7, but at day 14, this effect decreased despite phenylpropanolamine administration. No morphologic changes from baseline were detected after either treatment in any dog. CONCLUSIONS AND CLINICAL RELEVANCE: Data suggest that estriol mainly acts on the urethral sphincter mechanism by increasing urethral resistance in sexually intact and spayed female dogs without urinary incontinence. Administration of estriol and phenylpropanolamine did not increase the urethral resistance more than estriol alone. The urodynamic effects of estriol in female dogs with urinary incontinence remain to be elucidated [less ▲]

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See detailInvestigation of blood oxidant/antioxidant markers in healthy competition horses : effect of discipline and gender.
Kirschvink, N.; De Moffarts, Brieuc; Farnir, Frédéric ULg et al

in 7th International Conference on Equine Exercise Physiology (2006)

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See detailTransgenic engineering of male-specific muscular hypertrophy.
Pirottin, Dimitri ULg; Grobet, Luc ULg; Adamantidis, Antoine ULg et al

in Proceedings of the National Academy of Sciences of the United States of America (2005), 102(18), 6413-8

Using a two-step procedure involving insertional gene targeting and recombinase-mediated cassette exchange in ES cells, we have produced two lines of transgenic mice expressing a dominant-negative latency ... [more ▼]

Using a two-step procedure involving insertional gene targeting and recombinase-mediated cassette exchange in ES cells, we have produced two lines of transgenic mice expressing a dominant-negative latency-associated myostatin propeptide under control of the myosin light chain 1F promoter and 1/3 enhancer from the TSPY locus on the Y chromosome. Males of the corresponding lines are characterized by a 5-20% increase in skeletal muscle mass. This experiment demonstrates the feasibility of a more efficient cattle production system combining superior beef production abilities for bulls and dairy abilities for cows. [less ▲]

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See detailCaractérisation et maîtrise des paramètres de la reproduction et de la croissance des ovins Djallonké (Ovis amon aries)
Gbangboche, A. B.; Hornick, Jean-Luc ULg; Adamou-N'Diaye, M. et al

in Annales de Médecine Vétérinaire (2005), 149(3), 148-160

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See detailBovine herpesvirus 4 induces apoptosis of human carcinoma cell lines in vitro and in vivo
Gillet, Laurent ULg; Dewals, Benjamin G ULg; Farnir, Frédéric ULg et al

in Cancer Research (2005), 65(20), 9463-9472

The idea of using oncolytic viruses for the treatment of cancers was proposed a century ago. During the last two decades, viruses able to replicate specifically in cancer cells and to induce their lysis ... [more ▼]

The idea of using oncolytic viruses for the treatment of cancers was proposed a century ago. During the last two decades, viruses able to replicate specifically in cancer cells and to induce their lysis were identified and were genetically modified to improve their viro-oncolytic properties. More recently, a new approach consisting of inducing selective apoptosis in cancer cells through viral infection has been proposed; this approach has been called viro-oncoapoptosis. In the present study, we report the property of bovine herpesvirus-4 (BoHV-4) to induce, in vitro and in vivo, apoptosis of some human carcinomas. This conclusion relies on the following observations: (a) In vitro, BoHV-4 infection induced apoptosis of A549 and OVCAR carcinoma cell lines in a time- and dose-dependent manner. (b) Apoptosis was induced by the expression of an immediate-early or an early BoHV-4 gene, but did not require viral replication. (c) Cell treatment with caspase inhibitors showed that apoptosis induced by BoHV-4 relied mainly on caspase-10 activation. (d) Infection of cocultures of A549 or OVCAR cells mixed with human 293 cells (in which BoHV-4 does not induce apoptosis) showed that BoHV-4 specifically eradicated A549 or OVCAR cancer cells from the cocultures. (e) Finally, in vivo experiments done with nude mice showed that BoHV-4 intratumoral injections reduced drastically the growth of preestablished A549 xenografts. Taken together, these results suggest that BoHV-4 may have potential as a viro-oncoapoptotic agent for the treatment of some human carcinomas. Moreover, further identification of BoHV-4 proapoptotic gene(s) and the cellular pathways targeted by this or these gene(s) could lead to the design of new cancer therapeutic strategies. [less ▲]

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See detailGaseous emissions in the raising of weaned pigs on fully slatted floor or on sawdust-based deep litter
Nicks, Baudouin ULg; Laitat, Martine ULg; Farnir, Frédéric ULg et al

in Proceedings of the International workshop on Green Pork Production (2005)

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See detailReal-time RT-PCR quantification of mRNA encoding cytokines and chemokines in histologically normal canine nasal, bronchial and pulmonary tissue
Peeters, Dominique ULg; Peters, I. R.; Farnir, Frédéric ULg et al

in Veterinary Immunology and Immunopathology (2005), 104(3-4), 195-204

Cytokines and chemokines are likely to be involved in the pathogenesis of inflammatory diseases of the canine respiratory tract. The roles and relative amounts of these molecules have not yet been defined ... [more ▼]

Cytokines and chemokines are likely to be involved in the pathogenesis of inflammatory diseases of the canine respiratory tract. The roles and relative amounts of these molecules have not yet been defined in the respiratory mucosa of normal dogs or dogs with naturally acquired respiratory inflammation. In the present study, real-time reverse transcriptase polymerase chain reaction (RT-PCR) assays were employed to quantify messenger RNA (mRNA) encoding the chemokines monocyte chernotactic protein (MCP)-2, eotaxin-2 and eotaxin-3, and the cytokines interleukin-4 (IL-4), IL-5, IL-6, IL-10, IL-12p40, IL-18, interferon-gamma (IFN-gamma), tumour necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta (TGF-beta) in normal nasal, bronchial and pulmonary tissues from puppies (n = 4) and from adult dogs (n = 7). There was no significant difference in the expression of any transcript between puppies and adult dogs at any of the anatomical sites examined. The expression of mRNA encoding eotaxin-2 and eotaxin-3 increased significantly with progression from the nasal mucosa to pulmonary parenchyma but expression of MCP-2 mRNA did not show this trend. At all levels of the respiratory mucosa, the most abundant transcripts were those encoding IL-18 and TGF-beta. Transcripts encoding IL-6, IL-10, IL-12 and TNF-alpha were approximately ten-fold less abundant, and IL-4, IL-5 and IFN-gamma were the least abundant templates. There was significantly different amount of mRNA encoding IL-5, IL- 18 and TNF-alpha between particular anatomical levels of the respiratory mucosa while the mRNA expression of the other cytokines was similar at all anatomical sites. The results of the present study will enable comparisons to be made with results obtained from similar samples obtained from dogs with nasal, bronchial or pulmonary diseases. (c) 2004 Elsevier B.V. All rights reserved. [less ▲]

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See detailDistribution of leucocyte subsets in the canine respiratory tract
Peeters, Dominique ULg; Day, M. J.; Farnir, Frédéric ULg et al

in Journal of Comparative Pathology (2005), 132(4), 261-272

Histochemistry and immunohistochemistry were used to characterize leucocyte subsets in the respiratory tract of 15 outbred dogs (five aged <6 months and 10 aged >1 year) that had no evidence of ... [more ▼]

Histochemistry and immunohistochemistry were used to characterize leucocyte subsets in the respiratory tract of 15 outbred dogs (five aged <6 months and 10 aged >1 year) that had no evidence of respiratory disease. No organized nose- or bronchus-associated lymphoid tissue was observed in any of the sections examined. IgA(+) plasma cells predominated in nasal mucosa and in all parts of the bronchial tree, with fewer IgG(+) and IgM(+) plasma cells. The numbers of IgA(+) and IgM(+) cells were significantly greater in the nasal mucosa than in any other part of the respiratory mucosa. There were significantly fewer IgA(+), IgG(+) and IgM(+) cells in all parts of the respiratory tract in the puppies than in the adults. The number and distribution of mast cells and cells expressing MHC class II, L1 or CD1c were recorded. Mast cells were mainly found in the subepithelial lamina propria of nasal and bronchial mucosa and in the alveolar interstitium, and cells expressing IgE had a similar distribution. Mast cells were also present within muscle layers of the bronchial tree. The numbers of mast cells and MHC class II(+) cells were significantly greater in the nasal mucosa than in any other part of the respiratory mucosa. In the nose, carina and primary and secondary bronchus, there were significantly more mast cells and MHC class II(+) cells in puppies than in adult dogs, whereas the numbers of L1(+) cells and CD1c(+) cells in most sites were significantly greater in older dogs. There were significantly more CD3(+) and CD8(+) cells in the nasal mucosa than in any part of the bronchial mucosa. In most parts of the respiratory mucosa, CD4(+), CD8(+) and TCR alphabeta(+) cells were present in significantly greater numbers in adults than in puppies. All parts of the respiratory tract had similar numbers of mucosal CD4(+) and CD8(+) T lymphocytes. TCR gammadelta(+) cells were absent or sparse in all samples. These data, obtained from dogs without respiratory disease, will enable comparisons to be made with dogs suffering from infectious or inflammatory nasal, bronchial and pulmonary diseases. [less ▲]

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See detailCharacterisation and control of the reproduction and growth parameters of Djallonke ovines (Ovis amon aries)
Gbangboche, Armand; Hornick, Jean-Luc ULg; Adamou-N'Diaye, Maman et al

in Annales de Médecine Vétérinaire (2005), 149(3), 148-160

Plusieurs années d’efforts de recherches témoignent que l’élevage des ovins Djallonké est une activité majeure dans l’Afrique subsaharienne, si l’on en juge par la masse des connaissances produites. Les ... [more ▼]

Plusieurs années d’efforts de recherches témoignent que l’élevage des ovins Djallonké est une activité majeure dans l’Afrique subsaharienne, si l’on en juge par la masse des connaissances produites. Les auteurs, au travers d’un inventaire des différentes contributions scientifiques et techniques de 1967 à 2004, rapportent les performances de reproduction et de croissance des ovins Djallonké et les facteurs de leur variation. Les performances de reproduction ont été évaluées au travers de six critères zootechniques : cyclicité sexuelle annuelle, précocité sexuelle, intervalle entre agnelages, productions laitières, prolificité et mortalité des agneaux. Les performances de croissance ont été étudiées pendant deux périodes : la période d’allaitement naturel (croissance entre naissance et sevrage) et la période post sevrage. Les estimations des paramètres génétiques (héritabilité, corrélations génétiques et phénotypiques, répétabilité) de quelques caractères de reproduction et de croissance, ainsi que les résultats d’amélioration génétique, ont été passés en revue. Les conseils pratiques sont loin d’être uniformes étant donné la grande diversité des conditions d’expérimentation et d’analyse entre les différentes publications. [less ▲]

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See detailGaseous emissions in the raising of fattening pigs on fully slatted floor or on straw-based deep litter
Nicks, Baudouin ULg; Philippe, François-Xavier ULg; Laitat, Martine ULg et al

in Proceedings of the XIIth international congress of the International Society for animal Hygiene, Animals and the environment (2005)

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See detailEctopic expression of DLK1 protein in skeletal muscle of padumnal heterozygotes causes the callipyge phenotype
Davis, Erica; Jensen, C.; Schroder, H. D. et al

in Current Biology (2004), 14(20), 1858-1862

The callipyge (CLPG) phenotype is an inherited skeletal muscle hypertrophy described in sheep. It is characterized by an unusual mode of inheritance ("polar overdominance") in which only heterozygous ... [more ▼]

The callipyge (CLPG) phenotype is an inherited skeletal muscle hypertrophy described in sheep. It is characterized by an unusual mode of inheritance ("polar overdominance") in which only heterozygous individuals having received the CLPG mutation from their father (+(MAT)/CLPG(PAT)) express the phenotype . +(MAT)/CLPG(PAT) individuals are born normal and develop the muscular hypertrophy at approximately 1 month of age. The CLPG mutation was identified as an A to G transition in a highly conserved dodecamer motif located between the imprinted DLK1 and GTL2 genes . This motif is thought to be part of a long-range control element (LRCE) because the CLPG mutation was shown, in postnatal skeletal muscle, to enhance the transcript levels of the DLK1, PEG11, GTL2, and MEG8 genes in cis without altering their imprinting status . As a result, the +(MAT)/CLPG(PAT) individuals have a unique expression profile thought to underlie the callipyge phenotype: an overexpression of the paternally expressed protein encoding DLK1 (Figure 1A) and PEG11 transcripts in the absence of an overexpression of the maternally expressed noncoding GTL2 and MEG8 transcripts . However, the way in which this distinct expression profile causes the callipyge muscular hypertrophy has remained unclear. Herein, we demonstrate that the callipyge phenotype is perfectly correlated with ectopic expression of DLK1 protein in hypertrophied muscle of +(MAT)/CLPG(PAT) sheep. We demonstrate the causality of this association by inducing a generalized muscular hypertrophy in transgenic mice that express DLK1 in skeletal muscle. The absence of DLK1 protein in skeletal muscle of CLPG/CLPG animals, despite the presence of DLK1 mRNA, supports a trans inhibition mediated by noncoding RNAs expressed from the maternal allele. [less ▲]

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See detailGenetic and functional confirmation of the causality of the DGAT1 K232A quantitative trait nucleotide in affecting milk yield and composition
Grisart, B.; Farnir, Frédéric ULg; Karim, Latifa ULg et al

in Proceedings of the National Academy of Sciences of the United States of America (2004), 101(8), 2398-2403

We recently used a positional cloning approach to identify a nonconservative lysine to alanine substitution (K232A) in the bovine DGAT1 gene that was proposed to be the causative quantitative trait ... [more ▼]

We recently used a positional cloning approach to identify a nonconservative lysine to alanine substitution (K232A) in the bovine DGAT1 gene that was proposed to be the causative quantitative trait nucleotide underlying a quantitative trait locus (QTL) affecting milk fat composition, previously mapped to the centromeric end of bovine chromosome 14. We herein generate genetic and functional data that confirm the causality of the DGAT1 K232A mutation. We have constructed a high-density single-nucleotide polymorphism map of the 3.8-centimorgan BULGE30-BULGE9 interval containing the QTL and show that the association with milk fat percentage maximizes at the DGAT1 gene. We provide evidence that the K allele has undergone a selective sweep. By using a baculovirus expression system, we have expressed both DGAT1 alleles in Sf9 cells and show that the K allele, causing an increase in milk fat percentage in the live animal, is characterized by a higher V-max in producing triglycerides than the A allele. [less ▲]

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See detailProduction des ovins Djallonké en station dans la zone guinéenne au Bénin : 1. Performances de reproduction et influence des facteurs non génétiques
Gbangboche, A. B.; Hounzangbe-Adote, S. M.; Doko, S. Y. et al

in Revue Africaine de Santé et de Productions Animales [=RASPA] (2004), 2

Production of Djallonke sheep at an experimental station in the guinean zone of Benin. Reproduction performance and influence of non-genetic factors From 1994 to 2002, data from Djallonke sheep were ... [more ▼]

Production of Djallonke sheep at an experimental station in the guinean zone of Benin. Reproduction performance and influence of non-genetic factors From 1994 to 2002, data from Djallonke sheep were collected for reproduction performance. The data were obtained from the farm of the Faculty of Agronomic Sciences (University of Abomey Calavi). Data analysed by GLM procedure and Student’s t-test, were used to identify and quantify non-genetics factors affecting the age at first lambing, intervals between lambing and prolificacy. Results indicated: age at first lambing, 609.64days±110.26 days; intervals between lambing, 243.01days±68.19days; prolificity, 1.28±0.45; annual reproduction rate, 1.92%; temporary sterility rate, 5.79%. Lambing season, lambing year and the year*season interaction affected significantly (p<0.01) the age at first lambing and the interval between lambing. The same factors were not significant (p>0.05) for prolificacy. [less ▲]

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See detailGaseous emissions from deep-litter pens with straw or sawdust for fattening pigs
Nicks, Baudouin ULg; Laitat, Martine ULg; Farnir, Frédéric ULg et al

in Animal Science (2004), 78(Part 1), 99-107

Three successive batches of fattening pigs were raised on a deep litter of straw in one room and of sawdust in another. The quantities of litter used per pig were 40 kg of straw and 81 kg of sawdust. Once ... [more ▼]

Three successive batches of fattening pigs were raised on a deep litter of straw in one room and of sawdust in another. The quantities of litter used per pig were 40 kg of straw and 81 kg of sawdust. Once a month, the emissions of ammonia, nitrous oxide, methane, carbon dioxide and water vapour were measured continuously for 6 days consecutively. Gaseous emissions from pig raising on sawdust-based litter and straw-based litter were respectively 12.16 and 13.61 g per pig per day for ammonia (NH3), 4.96 and 7.39 g per pig per day for methane (CH4), 2.09 and 0.03 g per pig per day for nitrous oxide (N2O), 3.15 and 2.74 kg per pig per day for water (H2O) and 1.32 and 1.30 kg per pig per day for carbon dioxide (CO2). Differences between the emissions of the two litters were significant for N2O and H2O (P < 0.01). The nitrogen content of the manures collected at the end of the experiment was 1.47 kg per pig for the straw-based litter and 1.07 kg per pig for that based on sawdust. Nitrogen emissions were calculated under the assumption that no gases volatilized from the litter or from the animals other than NH3 and N2O. With the two litters, about 50% of nitrogen excreted by the pigs was emitted into the atmosphere in the form of N-2. [less ▲]

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