References of "Farnir, Frédéric"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailDemonstrating polymorphic miRNA-mediated gene regulation in vivo: application to the g+6223G->A mutation of Texel sheep.
Takeda, Haruko ULg; Charlier, Carole ULg; Farnir, Frédéric ULg et al

in RNA (New York, N.Y.) (2010), 16(9), 1854-63

We herein describe the development of a biochemical method to evaluate the effect of single nucleotide polymorphisms (SNPs) in target genes on their regulation by microRNAs in vivo. The method is based on ... [more ▼]

We herein describe the development of a biochemical method to evaluate the effect of single nucleotide polymorphisms (SNPs) in target genes on their regulation by microRNAs in vivo. The method is based on the detection of allelic imbalance in RNAs coimmunoprecipitated with AGO proteins from tissues of heterozygous individuals. We characterize the performances of our approach using a model system in a cell culture, and then apply it successfully to prove that the 3'UTR g+6223G-->A mutation operates by promoting RISC-dependent down-regulation of myostatin (MSTN) in skeletal muscle of Texel sheep. [less ▲]

Detailed reference viewed: 6 (2 ULg)
Full Text
Peer Reviewed
See detailBone Turnover During Pregnancy in Horses
Greiner, Claudia; Cavalier, Etienne ULg; Remy, Benoit et al

in Journal of Bone and Mineral Research (2010), 25

Detailed reference viewed: 47 (11 ULg)
Full Text
Peer Reviewed
See detailBovine herpesvirus 4 ORF73 is dispensable for viral growth in vitro but is essential for viral persistence in vivo.
Thirion, M.; Machiels, Bénédicte ULg; Farnir, Frédéric ULg et al

in Journal of General Virology (The) (2010), 91(10), 2574-84

ORF73 orthologues encoded by different rhadinoviruses have been studied extensively. These studies revealed that the ORF73 expression product (pORF73) is a multifunctional protein essential for latency ... [more ▼]

ORF73 orthologues encoded by different rhadinoviruses have been studied extensively. These studies revealed that the ORF73 expression product (pORF73) is a multifunctional protein essential for latency that enables episome tethering to mitotic chromosomes and modulates cellular pathways implicated in growth and survival of latently infected cells. Comparison of pORF73 orthologues encoded by rhadinoviruses reveals important variations in amino acid sequence length and composition. Bovine herpesvirus 4 (BoHV-4) encodes by far the shortest ORF73 orthologue with a size equivalent to only 22% of the largest orthologues. The present study focused on determining if BoHV 4 ORF73 is a bona fide gene and investigating whether it is essential for latency as established for larger ORF73 orthologues. Our results demonstrate that BoHV-4 ORF73 is transcribed as immediate-early bicistronic mRNA together with ORF71. Using a BoHV-4 bacterial artificial chromosome clone, we produced a strain deleted for ORF73 and a revertant strain. Deletion of BoHV-4 ORF73 did not affect the capacity of the virus to replicate in vitro, but it prevented latent infection in vivo using a rabbit model. Interestingly, the strain deleted for ORF73 induced an anti-BoHV-4 humoral immune response comparable to that elicited by wild-type and revertant recombinants. Together, these results demonstrate that despite its relatively small size, BoHV-4 ORF73 is a functional homologue of larger rhadinovirus ORF73 orthologues, and highlight the potential of ORF73 deletion for the development of BoHV-4 as a vector in vaccinology. [less ▲]

Detailed reference viewed: 73 (40 ULg)
Peer Reviewed
See detailMalignant catarrhal fever induced by alcelaphine herpesvirus 1 is associated with proliferation of CD8+ T cells supporting a latent infection
Dewals, Benjamin G ULg; Boudry, Christel; Farnir, Frédéric ULg et al

Poster (2009, September 11)

Alcelaphine herpesvirus 1 (AlHV 1), carried by wildebeest asymptomatically, causes malignant catarrhal fever (WD MCF) when cross species transmitted to a variety of susceptible species of the Artiodactyla ... [more ▼]

Alcelaphine herpesvirus 1 (AlHV 1), carried by wildebeest asymptomatically, causes malignant catarrhal fever (WD MCF) when cross species transmitted to a variety of susceptible species of the Artiodactyla order. Experimentally, WD-MCF can be induced in rabbits. The lesions observed are very similar to those described in natural host species. Here, we used the rabbit model and in vivo 5-Bromo-2’-Deoxyuridine (BrdU) incorporation to study WD-MCF pathogenesis. The results obtained can be summarized as follows. (i) AlHV-1 infection induces CD8+ T cell proliferation detectable as early as 15 days post-inoculation. (ii) While the viral load in peripheral blood mononuclear cells remains below the detection level during most of the incubation period, it increases drastically few days before death. At that time, at least 10% of CD8+ cells carry the viral genome; while CD11b+, IgM+ and CD4+ cells do not. (iii) RT-PCR analyses of mononuclear cells isolated from the spleen and the popliteal lymph node of infected rabbits revealed no expression of ORF25 and ORF9, low or no expression of ORF50, and high or no expression of ORF73. Based on these data, we propose a new model for the pathogenesis of WD-MCF. This model relies on proliferation of infected CD8+ cells supporting a predominantly latent infection. [less ▲]

Detailed reference viewed: 21 (10 ULg)
Peer Reviewed
See detailMalignant catarrhal fever induced by alcelaphine herpesvirus 1 is associated with proliferation of CD8+ T cells supporting a latent infection
Dewals, Benjamin G ULg; Boudry, Christel; Farnir, Frédéric ULg et al

Conference (2009, April)

Alcelaphine herpesvirus 1 (AlHV 1), carried by wildebeest asymptomatically, causes malignant catarrhal fever (WD MCF) when cross species transmitted to a variety of susceptible species of the Artiodactyla ... [more ▼]

Alcelaphine herpesvirus 1 (AlHV 1), carried by wildebeest asymptomatically, causes malignant catarrhal fever (WD MCF) when cross species transmitted to a variety of susceptible species of the Artiodactyla order. Experimentally, WD-MCF can be induced in rabbits. The lesions observed are very similar to those described in natural host species. Here, we used the rabbit model and in vivo 5-Bromo-2’-Deoxyuridine (BrdU) incorporation to study WD-MCF pathogenesis. The results obtained can be summarized as follows. (i) AlHV-1 infection induces CD8+ T cell proliferation detectable as early as 15 days post-inoculation. (ii) While the viral load in peripheral blood mononuclear cells remains below the detection level during most of the incubation period, it increases drastically few days before death. At that time, at least 10% of CD8+ cells carry the viral genome; while CD11b+, IgM+ and CD4+ cells do not. (iii) RT-PCR analyses of mononuclear cells isolated from the spleen and the popliteal lymph node of infected rabbits revealed no expression of ORF25 and ORF9, low or no expression of ORF50, and high or no expression of ORF73. Based on these data, we propose a new model for the pathogenesis of WD-MCF. This model relies on proliferation of infected CD8+ cells supporting a predominantly latent infection. [less ▲]

Detailed reference viewed: 21 (4 ULg)
Full Text
Peer Reviewed
See detailPerformances zootechniques de la poule Ardennaise, une race ancienne pour le futur ?
Moula, Nassim ULg; Antoine-Moussiaux, Nicolas ULg; Farnir, Frédéric ULg et al

in Annales de Médecine Vétérinaire (2009), 153(1), 66-75

Nowadays, Belgian poultry breeding is characterized by a strong biodiversity that is nevertheless greatly endangered. The present work aims at evaluating the zootechnical potential of an ancient poultry ... [more ▼]

Nowadays, Belgian poultry breeding is characterized by a strong biodiversity that is nevertheless greatly endangered. The present work aims at evaluating the zootechnical potential of an ancient poultry breed originating from the Franco-Belgian Ardennes region, the Ardennaise. This breed shows an important phenotypic variation, with 10 different varieties being recognized. This study evaluates the Ardennaise production performances and is divided into three parts: (1) the analysis of the growth and the consumption indices over a twelve weeks period, (2) the comparison of the growth over seventeen weeks in four varieties, (3) the study of eggs number and eggs weight over 70 weeks laying. The results indicate that the Ardennaise breed constitutes a good dual purpose breed that could be interestingly submitted to selection. Its use in industrial crosses for egg composition amelioration is also proposed. Lastly, this works shows the importance of extending the here developed evaluation protocol to other Belgian endangered poultry breeds to motivate the necessary conservation programs. [less ▲]

Detailed reference viewed: 384 (134 ULg)
Full Text
Peer Reviewed
See detailL’Ardennaise: statut de la race et de ses variétés.
Moula, Nassim ULg; Antoine-Moussiaux, Nicolas ULg; Farnir, Frédéric ULg et al

in Annales de Médecine Vétérinaire (2009), 153

Detailed reference viewed: 47 (24 ULg)
Full Text
Peer Reviewed
See detailEvaluation of the Production Performances of an Endangered Local Poultry Breed, the Famennoise
Moula, Nassim ULg; Antoine-Moussiaux, Nicolas ULg; Farnir, Frédéric ULg et al

in International Journal of Poultry Sciences (2009), 8(4), 389-396

The Famennoise is a Belgian poultry breed which is greatly endangered. Like most of the local breeds in this situation, the Famennoise remains largely unknown and is representative of the continuous loss ... [more ▼]

The Famennoise is a Belgian poultry breed which is greatly endangered. Like most of the local breeds in this situation, the Famennoise remains largely unknown and is representative of the continuous loss of genetic diversity that is threatening the future of animal production. From preliminary results, egg production traits in this breed showed valuable economic assets. The present study is, thus, aimed at assessing its production performances with the prospect that it might be conserved for future valorization. Egg production as well as growth traits were estimated. Both aspects showed exploitable performances. In absence of past selection for these traits, eggs presented a mean weight of 55.433.03 g, so being in the middle class of marketable eggs, a yolk to albumen ratio of 50.75.02%, an eggshell resistance (maximal force of breakage of 36.033.3 N) equal to commercial strains and superior to already valorized local breeds. In broilers, a mean weight 980.6716.62 g was reached at 8 weeks, 1815.9036.55 g at week 12 and 2191.9048.31 g at week 15. The Famennoise is, therefore, suggested for use as a dual-purpose breed with a good potential of selection for both productions. It could further serve in crosses for improvement of commercial strains. In conclusion, it appears to be highly urgent to screen endangered local poultry breeds for economically exploitable traits which would motivate conservation programs of biodiversity, before this extraordinary scientific and economic potential get irremediably lost. [less ▲]

Detailed reference viewed: 125 (57 ULg)
Full Text
Peer Reviewed
See detailComparison of Egg Composition and Conservation Ability in Two Belgian Local Breeds and One Commercial Strain
Moula, Nassim ULg; Antoine-Moussiaux, Nicolas ULg; Farnir, Frédéric ULg et al

in International Journal of Poultry Sciences (2009), 8(8), 768-774

The study was conducted to evaluate the egg quality of two Belgian local chicken breeds (the Ardennaise and the Famennoise) and to compare it with a commonly bred commercial strain (Lohmann brown). Two ... [more ▼]

The study was conducted to evaluate the egg quality of two Belgian local chicken breeds (the Ardennaise and the Famennoise) and to compare it with a commonly bred commercial strain (Lohmann brown). Two criteria were used to describe egg quality: egg composition (measured through yolk to albumen ratio) and its stability during conservation (measured through pH). Egg weight, size, composition as well as albumen pH were measured on 140 eggs. Measurements were performed at days 0, 7, 14 and 21 after laying. The egg weight was highest in the Lohmann strain (62.86 g), followed by the Famennoise breed (55.51 g) and lowest in the Ardennaise (50.31 g). Yolk to albumen ratio was significantly higher in the Ardennaise (0.53) when compared with the Famennoise (0.49) and Lohmann breeds (0.43). Albumen pH at laying was lowest in the Lohmann when compared with the local breeds. However, no statistical differences among the three breeds could be detected for this parameter at days 14 or 21 postlaying. The high pH in local breed eggs is often reported to be caused by a lower conservation ability. The present results suggest some physiologically higher pH at laying not resulting from defaults in conservation. As a measure of egg freshness, pH must be adapted by the various poultry breeds. [less ▲]

Detailed reference viewed: 104 (46 ULg)
Peer Reviewed
See detailEffect of breed on Doppler echocardiographic measurements in equids
Al Haidar, A; Sandersen, Charlotte ULg; Van Erck, Emmanuelle et al

in Proceedings of the 3rd Annual Congress of the European College of Equine Internal Medicine (ECEIM) (2009)

Detailed reference viewed: 7 (2 ULg)
Full Text
Peer Reviewed
See detailReproductive Performance of the Ardennaise Chicken Breed under Traditional and Modern Breeding Management Systems
Larivière, Jean-Marc ULg; Michaux, Charles ULg; Farnir, Frédéric ULg et al

in International Journal of Poultry Sciences (2009), 8(5), 446-451

Enhancing the use of traditional chicken breeds is necessary to insure long-term conservation of global genetic diversity in poultry. A logical start in this process is to evaluate performance. The ... [more ▼]

Enhancing the use of traditional chicken breeds is necessary to insure long-term conservation of global genetic diversity in poultry. A logical start in this process is to evaluate performance. The objective of this study was to estimate reproductive traits in the Ardennaise chicken breed under traditional and modern management systems. Reproductive performance indicated lighting programs [Natural (NAT) or Artificial (ART) day length] were highly significant for egg weight, hatchability (p<0.001), early and mid-term embryonic mortality (p<0.01). Feeding treatments [ad libitum (AL) or mild Feed-restriction (FR)] varied greatly with egg weight, hatchability, fertility (p<0.001) and late embryonic mortality (p<0.05). Most precocious age at first egg (23 weeks), longest duration of laying period (>27 weeks), utmost maximum production peak (56.67%), largest egg number (70.13 eggs/hen) and number of chicks hatched (24.10 chicks/hen) were performed under ART-AL. Heaviest egg weight (53.21g), highest fertility (70.20%), greatest hatchability (55.58%), highest liveability during rearing (95.74%) and lay (100%), lowest early (3.51%) and mid-term embryonic mortality (1.17%) were achieved under NAT-AL. To summarize, most of traits studied in the Ardennaise chicken were moderate when compared to those expected by broiler breeders. [less ▲]

Detailed reference viewed: 161 (19 ULg)
Full Text
Peer Reviewed
See detailCo-infection with two closely related alphaherpesviruses results in a highly diversified recombination mosaic displaying negative genetic interference
Muylkens, Benoît ULg; Farnir, Frédéric ULg; Meurens, François et al

in Journal of Virology (2009), 83(7), 3127-3137

Phylogenetic studies of the emergence and spread of natural recombinants in herpesviruses infecting humans and animals have been reported recently. However, despite an ever-increasing amount of evidence ... [more ▼]

Phylogenetic studies of the emergence and spread of natural recombinants in herpesviruses infecting humans and animals have been reported recently. However, despite an ever-increasing amount of evidence of recombination in herpesvirus history, the recombination process and the consequences on the genetic diversity of the progeny remain poorly characterized. We addressed this issue by using multiple single-nucleotide polymorphisms (SNPs) differentiating the two subtypes of an alphaherpesvirus, bovine herpesvirus 1 (BoHV-1). Analysis of a large sample of progeny virions obtained in a single growth cycle of coinfected BoHV-1 strains provided a prospective investigation of the recombination dynamics by using SNPs as recombination markers. We found that the simultaneous infection with two closely related herpesviruses results in a highly diversified recombination mosaic. From the analysis of multiple recombinants arising in the progeny, we provide the first evidence of genetic interference influencing the recombination process in herpesviruses. In addition, we report striking differences in the levels of recombination frequency observed along the BoHV-1 genome. With particular emphasis on the genetic structure of a progeny virus population rising in vitro, our data show to which extent recombination participates to the genetic diversification of herpesviruses [less ▲]

Detailed reference viewed: 80 (27 ULg)
Full Text
Peer Reviewed
See detailMapping QTL influencing gastrointestinal nematode burden in Dutch Holstein-Friesian dairy cattle.
Coppieters, Wouter ULg; Mes, Ted H M; Druet, Tom ULg et al

in BMC Genomics (2009), 10

BACKGROUND: Parasitic gastroenteritis caused by nematodes is only second to mastitis in terms of health costs to dairy farmers in developed countries. Sustainable control strategies complementing ... [more ▼]

BACKGROUND: Parasitic gastroenteritis caused by nematodes is only second to mastitis in terms of health costs to dairy farmers in developed countries. Sustainable control strategies complementing anthelmintics are desired, including selective breeding for enhanced resistance. RESULTS AND CONCLUSION: To quantify and characterize the genetic contribution to variation in resistance to gastro-intestinal parasites, we measured the heritability of faecal egg and larval counts in the Dutch Holstein-Friesian dairy cattle population. The heritability of faecal egg counts ranged from 7 to 21% and was generally higher than for larval counts. We performed a whole genome scan in 12 paternal half-daughter groups for a total of 768 cows, corresponding to the approximately 10% most and least infected daughters within each family (selective genotyping). Two genome-wide significant QTL were identified in an across-family analysis, respectively on chromosomes 9 and 19, coinciding with previous findings in orthologous chromosomal regions in sheep. We identified six more suggestive QTL by within-family analysis. An additional 73 informative SNPs were genotyped on chromosome 19 and the ensuing high density map used in a variance component approach to simultaneously exploit linkage and linkage disequilibrium in an initial inconclusive attempt to refine the QTL map position. [less ▲]

Detailed reference viewed: 21 (8 ULg)
Full Text
Peer Reviewed
See detailSalmonella surveillance and control at post harvest in the Belgian pork meat chain.
Delhalle, Laurent ULg; Saegerman, Claude ULg; Farnir, Frédéric ULg et al

in Food Microbiology (2009), 26

Salmonella remains the primary cause of reported bacterial food borne disease outbreaks in Belgium. Pork and pork products are recognized as one of the major sources of human salmonellosis. In contrast ... [more ▼]

Salmonella remains the primary cause of reported bacterial food borne disease outbreaks in Belgium. Pork and pork products are recognized as one of the major sources of human salmonellosis. In contrast with the primary production and slaughterhouse phases of the pork meat production chain, only a few studies have focussed on the post-harvest stages. The goal of this study was to evaluate Salmonella and Escherichia coli contamination at the Belgian post-harvest stages. E. coli counts were estimated in order to evaluate the levels of faecal contamination. The results of bacteriological analysis from seven cutting plants, four meat-mincing plants and the four largest Belgian retailers were collected from official and self-monitoring controls. The prevalence of Salmonella in the cutting plants and meat-mincing plants ranged from 0% to 50%. The most frequently isolated serotype was Salmonella typhimurium. The prevalence in minced meat at retail level ranged from 0.3% to 4.3%. The levels of Salmonella contamination estimated from semi-quantitative analysis of data relating to carcasses, cuts of meat and minced meat were equal to 3.40 2.04 log CFU/cm2, 2.64 1.76 log CFU/g and 2.35 1.09 log CFU/g, respectively. The E. coli results in meat cuts and minced meat ranged from 0.21 0.50 to 1.23 0.89 log CFU/g and from 1.33 0.58 to 2.78 0.43 log CFU/g, respectively. The results showed that faecal contamination still needs to be reduced, especially in specific individual plants. [less ▲]

Detailed reference viewed: 95 (24 ULg)
See detailMarker assisted selection of bovine for improved milk composition.
Blott, Sarah; Kim; Schmidt-Kuntzel, Anne et al

Patent (2008)

The present invention provides a method of genotyping bovine for improved milk production traits by determining the GHR genotypic state of said bovine, wherein the GHR gene and polymorphisms within said ... [more ▼]

The present invention provides a method of genotyping bovine for improved milk production traits by determining the GHR genotypic state of said bovine, wherein the GHR gene and polymorphisms within said gene have been found to be associated with such improved milk production traits. [less ▲]

Detailed reference viewed: 18 (4 ULg)