References of "FOIDART, Jean-Michel"
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See detailSearch for anti-laminin antibodies in the serum of workers exposed to cadmium, mercury vapour or lead.
Bernard, A. M.; Roels, H. R.; Foidart, Jean-Michel ULg et al

in International Archives of Occupational and Environmental Health (1987), 59(3), 303-309

Anti-laminin antibodies were sought for in the serum of workers exposed to mercury vapour (Hg, n = 58), lead (Pb, n = 38) or cadmium (Cd, n = 47). Thirty-one workers removed from Cd exposure for an ... [more ▼]

Anti-laminin antibodies were sought for in the serum of workers exposed to mercury vapour (Hg, n = 58), lead (Pb, n = 38) or cadmium (Cd, n = 47). Thirty-one workers removed from Cd exposure for an average of eight years were also examined. Compared with control workers matched for age and socio-economic status, the prevalence of circulating anti-laminin antibodies was not increased in workers exposed to Hg (mean duration of exposure: 7.9 years and mean urinary excretion of Hg: 72 micrograms/g creatinine) nor in those exposed to Pb (mean duration of exposure: 10.6 years and mean Pb levels in blood: 535 micrograms/l). In contrast, anti-laminin antibodies were significantly more prevalent in Cd-exposed workers whose urinary Cd exceeded 20 micrograms/g creatinine. This observation was made in both currently exposed workers and in workers removed from Cd exposure (mean duration of exposure: 9.4 and 24.6 years and mean urinary Cd: 7.8 and 13.4 micrograms/g creatinine respectively). These autoantibodies were found in Cd workers with normal renal function as well as in those with increased proteinuria. [less ▲]

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See detailDemonstration of anti-laminin antibodies in the serum of healthy subjects
Bernard, A.; Passelecq, B.; Foidart, Jean-Michel ULg et al

in Biomedicine & Pharmacotherapy (1987), 41(4), 183-6

Antibodies directed against laminin, a glycoprotein of basement membranes, have been sought for in the sera of 347 healthy subjects by a method relying on the agglutination of laminin-coated latex ... [more ▼]

Antibodies directed against laminin, a glycoprotein of basement membranes, have been sought for in the sera of 347 healthy subjects by a method relying on the agglutination of laminin-coated latex particles. Fifty-five subjects (15.9%) hae been found to be positive, 37 with a titre of 2, 14 with a titre of 4 and 4 with a titer of 8. Applied to 106 patients whose a renal biopsy had been examined by immunofluorescence, the test yielded positive results in 91% of patients with linear deposits as compared to 10.3 and 15.4% respectively in patients presenting with and without granular deposits along the glomerular basement membrane. The titers in anti-laminin antibodies observed in these patients ranged from 2 to 8. The discovery of circulating anti-laminin antibodies in serum from subjects without clinical or subclinical sign of renal impairment rises several questions regarding the pathogenicity of these auto-antibodies and their possible association with genetic or environmental factors. [less ▲]

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See detailEvidence for basement membranes in rat tail tendon sheaths.
Guizzardi, S.; Foidart, Jean-Michel ULg; Leonardi, L. et al

in Basic and Applied Histochemistry (1987), 31(2), 177-81

The presence of anti-laminin antibodies and a basement membrane-like thin electrondense lamina has been demonstrated in the peritendineum of the rat tail tendon by indirect immunofluorescence and electron ... [more ▼]

The presence of anti-laminin antibodies and a basement membrane-like thin electrondense lamina has been demonstrated in the peritendineum of the rat tail tendon by indirect immunofluorescence and electron microscopy. [less ▲]

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See detailUrinary excretion of neutral proteinases in nephrotic rats with a glomerular disease.
Davin, J. C.; Davies, M.; Foidart, Jean-Michel ULg et al

in Kidney International (1987), 31(1), 32-40

A proliferative glomerulonephritis was induced in rats pre-immunized with rabbit IgG by injecting intravenously a sub-nephrotoxic dose of rabbit anti-glomerular basement membrane (GBM) IgG (A rats). Most ... [more ▼]

A proliferative glomerulonephritis was induced in rats pre-immunized with rabbit IgG by injecting intravenously a sub-nephrotoxic dose of rabbit anti-glomerular basement membrane (GBM) IgG (A rats). Most rats (80%) developed a severe proteinuria (greater than 100 mg/24 hr) within two to five days after the injection of anti-GBM IgG. At the same time, microscopic examination of the kidneys revealed a glomerular infiltration by mononuclear phagocytes and a prominent decrease in the intensity of the colloidal iron reaction in glomeruli. A non-proliferative glomerular disease was induced in another group of rats (B rats) by intraperitoneal administration of aminonucleoside of puromycin. A marked proteinuria (greater than 100 mg/24 hr) occurred after six days in 90% of animals. Histochemical studies then revealed a decrease in staining intensity of glomeruli for polyanion. No glomerular hypercellularity was noted. In normal rats and in non-proteinuric A or B rats, the 24 hour urinary excretion of neutral proteinases ranged from 1.4 to 7.8 units (mean value +/- SEM, 4.69 +/- 0.60, N = 11), that of laminin ranged from 100 to 3,900 ng (mean value +/- SEM, 1,154 +/- 325, N = 10), and that of type IV collagen ranged from 160 to 420 ng (mean value +/- SEM, 306 +/- 26.5 ng, N = 8). In proteinuric rats from groups A (N = 11) and B (N = 9), the 24 hour urinary excretion of neutral proteinases significantly increased (mean values +/- SEM, 38.55 +/- 8.66 U for A rats and 42.17 +/- 7.92 U for B rats) and ran parallely with that of proteins, laminin and type IV collagen. [less ▲]

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See detailLe mélanome B16 de la souris en culture à trois dimensions
De Pauw-Gillet, Marie-Claire ULg; Christiane, Y.; Foidart, Jean-Michel ULg et al

in Bulletin de l'Association des Anatomistes (1986), 70(210), 21-4

Multicellular spheroids composed of mouse B16 melanoma cells have been prepared in non agitated culture on solid agar. Cells present frequent contacts and an extracellular matrix appears during ... [more ▼]

Multicellular spheroids composed of mouse B16 melanoma cells have been prepared in non agitated culture on solid agar. Cells present frequent contacts and an extracellular matrix appears during cultivation (up to 2 months in agitated suspension); it contains laminin, fibronectin and collagen I, III and IV. Cellular differentiation takes place inside these spheroids (melanogenesis progressively increases), but a relatively intense cell proliferation rate is also maintained. [less ▲]

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See detailFlavonoids : tools for the study of tumor invasion in vitro.
Bracke, M. E.; Van Cauwenberge, Jean-Rémy ULg; Mareel, M. M. et al

in Plant Flavonoids in Biology and Medicine: Biochemical, Pharmacological and Structure-Activity Relationships. (1986)

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See detailAn enzyme-linked immunoassay for direct measurement of the gelatin-binding capacity of human plasma fibronectin
Damas, Pierre ULg; Adam, A.; Closset, Jean ULg et al

in Journal of Immunological Methods (1986), 91

A new solid-phase enzyme immunoassay measuring the gelatin-binding capacity of plasma fibronectin has been developed. This assay is based on the direct and high-affinity interaction between fibronectin ... [more ▼]

A new solid-phase enzyme immunoassay measuring the gelatin-binding capacity of plasma fibronectin has been developed. This assay is based on the direct and high-affinity interaction between fibronectin and gelatin coated to polyvinyl chloride plates. The amount of fibronectin bound to gelatin is then measured by sequential incubation with a specific rabbit anti-human fibronectin antiserum, with horseradish peroxidase-conjugated goat anti-rabbit IgG antibodies and with substrate. The final degradation of the substrate is read at 492-650 nm in an ELISA processor. The assay allows the accurate detection of fibronectin concentrations ranging from 1 to 20 micrograms/ml, is inhibited by the addition of gelatin to plasma, is highly reproducible (interplate CV less than 10%), requires 100 microliter of plasma only and has been fully automated. Significant linear correlations were noted between total antigenic fibronectin (measured by laser nephelometry) and fibronectin gelatin-binding capacity in plasma from 310 blood donors. Both parameters were higher in men than in women and significantly increased according to age. Dissociation between immunoreactive fibronectin and fibronectin gelatin-binding capacity was observed in two polytraumatized patients. This enzyme immunoassay therefore provides a new method to investigate functional alterations of the gelatin-binding domain of fibronectin in various pathological conditions. [less ▲]

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See detailDevelopmental changes in interstitial collagens of fetal rat genital ducts
Paranko, J.; Foidart, Jean-Michel ULg; Pelliniemi, L. J.

in Developmental Biology (1986), 113(2), 364-372

The distribution of interstitial collagen types I and III was studied by immunocytochemistry in the mesenchyme of progressing and regressing mesonephric and paramesonephric ducts of male and female rat ... [more ▼]

The distribution of interstitial collagen types I and III was studied by immunocytochemistry in the mesenchyme of progressing and regressing mesonephric and paramesonephric ducts of male and female rat fetuses from the age of 15 days until birth. Immunocytochemistry revealed a collagen-poor mesenchymal area around the genital ducts and in continuation with the coelomic epithelium on the lateral edge of the mesonephric ridge of 15-day-old fetuses. Ultrastructurally, collagen fibrils were accumulated along the continuous lamina densa of the mesonephric ducts, whereas they were absent on the medial side of the male and female paramesonephric ducts. In males, the amount of collagen fibrils increased with the histological maturation of the mesenchyme around the mesonephric duct, whereas around the regressing paramesonephric duct collagens disappeared from the basement membrane region and the surrounding mesenchyme of the 16-day-old male duct. After the completion of the paramesonephric regression, the mesenchyme acquired a uniformly collagen containing interstitial matrix. In females, the collagens increased in the mesenchyme around the progressing paramesonephric duct, and the original site of the regressing mesonephric duct became occupied with a collagen-containing mesenchyme by the age of 19 days. The results suggest a close structural linkage between the mesonephric duct and the established early paramesonephric duct. The differences in the developmental maturation of the periductal mesenchyme and the observed changes in the composition of the interstitial matrix probably reflect the functional differences in the regulatory factors acting on the progression and regression of the male and female genital ducts. [less ▲]

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See detailDistribution of basement membrane antigens in glomeruli of mice with autoimmune glomerulonephritis.
Matsuo, S.; Brentjens, J. R.; Andres, G. et al

in American Journal of Pathology (1986), 122(1), 36-49

Glomerulonephritis was induced in mice by the repeated injection of human glomeruli or purified glomerular basement membrane. The glomerular basement membranes of nephritic animals were observed to ... [more ▼]

Glomerulonephritis was induced in mice by the repeated injection of human glomeruli or purified glomerular basement membrane. The glomerular basement membranes of nephritic animals were observed to develop subepithelial extensions, "spikes." Although normally Type IV collagen is found throughout the full thickness of basement membranes, the "spikes" reacted with antibodies to laminin but not with antibodies to Type IV collagen. It is proposed that in murine autoimmune glomerulonephritis, the visceral epithelial cells produce an excess of laminin. [less ▲]

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See detailHuman chondrocytes in tridimensional culture.
Bassleer, C.; Gysen, Ph; Foidart, Jean-Michel ULg et al

in In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association (1986), 22

Cartilage was taken from the macroscopically normal part of human femoral heads immediately after orthopedic surgical operations for total prothesis consecutive to hip arthrosis. After clostridial ... [more ▼]

Cartilage was taken from the macroscopically normal part of human femoral heads immediately after orthopedic surgical operations for total prothesis consecutive to hip arthrosis. After clostridial collagenase digestion and repeated washings, chondrocytes (10(6) cells) were cultivated in a gyrotory shaker (100 rpm). Under these conditions, cells were kept in suspension and after 3 to 5 d formed a flaky aggregate which, on Day 10, became dense. These chondrocytes were morphologically differentiated: they had a round shape, were situated inside cavities, and were surrounded by a new matrix. Histochemical methods showed the presence of collagen and polysaccharides in cell cytoplasm and in intercellular matrix, and the immunofluorescence method using specific antisera (anticartilage proteoglycans and anti-type II collagen) showed that these two constituents were in intercellular matrix. The measurement of the amounts of proteoglycans (PG) released into culture medium and those present in chondrocyte aggregate (by a specific PG radioimmunoassay) showed a maximum production on Days 3 to 5 of culture, then the production decreased and stabilized (from Day 10 to the end of culture). The observed difference between the amounts of PG in aggregates after 20 d and those after 2 h of culture demonstrated that PG neosynthesis did occur during cultivation. This conclusion was supported by other results obtained by [14C]glucosamine incorporation in chondrocyte aggregates. Moreover, the aggregate fresh weight related to cell number (appreciated by DNA assay) increased significantly with culture duration. Three-dimensional chondrocyte culture represents an interesting model: chondrocytes were differentiated morphologically as well as biosynthetically and synthesized a new cartilage matrix. [less ▲]

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See detailDistribution of extracellular matrix glycoproteins during normal development of human kidney. An immunohistochemical study.
Mounier, F.; Foidart, Jean-Michel ULg; Gubler, M. C.

in Laboratory Investigation : Journal of Technical Methods & Pathology (1986), 54(4), 394-401

The distribution of types I, III, and IV collagens, fibronectin, laminin, and heparan sulfate proteoglycan during human fetal kidney development has been studied by indirect immunofluorescence ... [more ▼]

The distribution of types I, III, and IV collagens, fibronectin, laminin, and heparan sulfate proteoglycan during human fetal kidney development has been studied by indirect immunofluorescence. Fibronectin and interstitial collagens types I and III are present in undifferentiated mesenchyme, whereas the intrinsic basement membrane components, type IV collagen, laminin, and heparan sulfate proteoglycan are not detected. In differentiated cortex, types I and III collagens are detected only as interstitial connective fibers, whereas fibronectin is found in both interstitium and glomeruli where its distribution is dependent on the stage of maturation. It is found in both the mesangium and along capillary walls of immature glomeruli, and principally in the mesangium of mature ones. Basement membrane components delineated the branching ureteric bud. They also outlined the structures of the earliest stage of nephrogenesis (epithelial differentiation). In mature nephrons, they are found along glomerular, capsular, and tubular basement membranes. [less ▲]

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See detailAntibodies to laminin in preeclampsia.
Foidart, Jean-Michel ULg; Hunt, J.; Lapière, C. M. et al

in Kidney International (1986), 29(5), 1050-57

Laminin is a large basement membrane glycoprotein localized in the trophoblast, glomerular basement membrane and in the mesangial matrix of human glomeruli. It promotes the attachment of epithelial cells ... [more ▼]

Laminin is a large basement membrane glycoprotein localized in the trophoblast, glomerular basement membrane and in the mesangial matrix of human glomeruli. It promotes the attachment of epithelial cells to basement membrane collagen. We have found that 14 sera from 52 patients with severe preeclampsia or eclampsia contain IgG and IgM antibodies which react with placental and kidney basement membranes. These antibodies were specific for laminin and did not react with other basement membrane proteins. They were able to fix complement. They have been demonstrated by radial immunodiffusion, radioimmunoassay and immunofluorescence blocking studies. In primary cultures they were shown to impair the attachment of trophoblast cells to basement membrane collagen. High levels of circulating immune complexes were detected only in sera from preeclamptic patients with circulating antibodies to laminin. The auto-antibodies to laminin could play a major role in the pathogenesis of severe preeclampsia by impairing the attachment of trophoblast cells to placental basement membranes and by fixation to the glomerular basement membranes and mesangial matrix. [less ▲]

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See detailAnti-basement-membrane antibodies in the serum of healthy subjects.
Bernard, A.; Lauwerys, R.; Mahieu, P. et al

in New England Journal of Medicine [=NEJM] (1986), 314(22), 1456-7

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See detailReticular fibroblasts in peripheral lymphoid organs identified by a monoclonal antibody.
Van Vliet, E.; Melis, M.; Foidart, Jean-Michel ULg et al

in Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society (1986), 34(7), 883-90

We have produced a panel of monoclonal antibodies directed against nonlymphoid cells in central and peripheral lymphoid organs. In this paper we present the reactivity of one of these antibodies, ER-TR7 ... [more ▼]

We have produced a panel of monoclonal antibodies directed against nonlymphoid cells in central and peripheral lymphoid organs. In this paper we present the reactivity of one of these antibodies, ER-TR7. This antibody detects reticular fibroblasts, which constitute the cellular framework of lymphoid and nonlymphoid organs and their products. In frozen sections of the spleen incubated with this antibody, the red pulp and white pulp are clearly delineated. Furthermore, the major white pulp compartments--the follicles and periarteriolar lymphoid sheath as well as the marginal zone--are recognized by their characteristic labeling patterns. In lymph nodes, the capsule, sinuses, follicles, paracortex, and medullary cords are clearly delineated. In the thymus and bone marrow no such specialized compartments were demonstrated. ER-TR7 reacts with an intracellular component of fibroblasts. Since ER-TR7 does not react with purified laminin, collagen types I-V, fibronectin, heparan sulfate proteoglycan, entactin, or nidogen, it detects a hitherto uncharacterized antigen. The possible role of the ER-TR7 positive reticular fibroblasts in the cellular organization of peripheral lymphoid organs will be discussed. [less ▲]

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See detailDetection of anti-laminin antibodies in sera by latex agglutination.
Bernard, A. M.; Foidart, Jean-Michel ULg; Mahieu, P. et al

in Clinical Chemistry (1986), 32(8), 1468-72

We describe a latex particle agglutination assay for detecting circulating antibodies against laminin, a noncollagenous glycoprotein of basement membranes. Polystyrene latex particles on which laminin has ... [more ▼]

We describe a latex particle agglutination assay for detecting circulating antibodies against laminin, a noncollagenous glycoprotein of basement membranes. Polystyrene latex particles on which laminin has been adsorbed are incubated with serum for about 25 min at 42-45 degrees C. The agglutination is then measured by counting residual unagglutinated particles. Polyethylene glycol 6000 enhances the agglutination. The assay is fully automated, yielding results in about 45 min, for 50 samples per hour. Addition of purified laminin abolishes the agglutination of laminin-coated particles in practically all positive sera. The anti-laminin antibody titers obtained by this latex immunoassay and by radioimmunoassay correlated well in 161 sera from patients with suspected or established renal diseases. The agglutination assay more frequently gave positive results for cases of glomerulonephritis with linear deposits (20/22 cases) than for glomerulonephritis with granular deposits (7/68) or glomerulonephritis with no glomerular deposits (2/13). The finding of low anti-laminin antibody titers in sera from about 15% (34/230) of the healthy subjects suggests that these autoantibodies are pathogenic only in certain circumstances. [less ▲]

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See detailLaminin promotes cerebellar granule cells migration in vitro and is synthesized by cultured astrocytes
SELAK, Ivan ULg; Foidart, Jean-Michel ULg; Moonen, Gustave ULg

in Developmental Neuroscience (1985), 7(5-6), 278-285

Newborn rat cerebellum microexplants have been used as a model to study neuronal migration. Laminin in a substrate-bound form modifies extensively the migratory behavior of the neurones, an effect which ... [more ▼]

Newborn rat cerebellum microexplants have been used as a model to study neuronal migration. Laminin in a substrate-bound form modifies extensively the migratory behavior of the neurones, an effect which is blocked if antilaminin antibodies are present during the assay. 35S methionine incorporation followed by SDS-PAGE electrophoresis, fluorography and immunoprecipitation with antilaminin antibodies allowed to demonstrate that laminin is synthesized and secreted by cultured newborn rat cerebellum as well as by cultured newborn rat cerebral cortex astrocytes. [less ▲]

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See detailB16 melanoma cells in bi- or tridimensional cultures
De Pauw-Gillet, Marie-Claire ULg; Simonon, Anne-Catherine; Borlon, Anne et al

in Archives de Biologie (1985)

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See detailFibronectin mediates the retraction of type I collagen fibers by breast cancer cells.
Castronovo, Vincenzo ULg; Paye, M.; Courtois, R. et al

in Evaluation du Risque de Cancer Mammaire. Chimiothérapie Première ? (1985)

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See detailInteraction between human breast adenocarcinoma cells and type I collagen matrix requires fibronectin.
Castronovo, Vincenzo ULg; Foidart, Jean-Michel ULg

in Biology and Chemistry of Basement Membranes. (1985)

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See detailInteractions of human breast adenocarcinoma with the extracellular matrix - a new concept to study cancer invasion.
Foidart, Jean-Michel ULg; Verly, M.; Castronovo, Vincenzo ULg et al

in Evaluation du Risque de Cancer Mammaire. Chimiothérapie Première ? (1985)

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