References of "FOIDART, Jean-Michel"
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See detailIn vivo biocompatibility of three potential intraperitoneal implants
Defrère, Sylvie; Mestdagt, Mélanie ULg; Riva, Raphaël ULg et al

in Macromolecular Bioscience (2011), 11(10), 1335-45

The intraperitoneal biocompatibility of PDMS, polyHEMA and pEVA was investigated in rats, rabbits and rhesus monkeys. No inflammation was evidenced by hematological analyses and measurement of ... [more ▼]

The intraperitoneal biocompatibility of PDMS, polyHEMA and pEVA was investigated in rats, rabbits and rhesus monkeys. No inflammation was evidenced by hematological analyses and measurement of inflammatory markers throughout the experiment and by post-mortem examination of the pelvic cavity. After 3 or 6 months, histological analysis revealed fibrous tissue encapsulating PDMS and PEVA implants in all species and polyHEMA implants in rabbits and monkeys. Calcium deposits were observed inside polyHEMA implants. The intraperitoneal biocompatibility of all 3 polymers makes them suitable for the design of drug delivery systems, which may be of great interest for pathologies confined to the pelvic cavity. [less ▲]

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See detailUnimpeded skin carcinogenesis in K14-HPV16 transgenic mice deficient for plasminogen activator inhibitor
Masset, Anne; Maillard, Catherine ULg; Sounni, Nor Eddine ULg et al

in International Journal of Cancer = Journal International du Cancer (2011), 128(2), 283-93

Angiogenesis, extracellular matrix remodeling and cell migration are associated with cancer progression and involve at least, the plasminogen activating system and its main physiological inhibitor, the ... [more ▼]

Angiogenesis, extracellular matrix remodeling and cell migration are associated with cancer progression and involve at least, the plasminogen activating system and its main physiological inhibitor, the plasminogen activator inhibitor-1 (PAI-1). Considering the recognized importance of PAI-1 in the regulation of tumor angiogenesis and invasion in murine models of skin tumor transplantation, we explored the functional significance of PAI-1 during early stages of neoplastic progression in the transgenic mouse model of multistage epithelial carcinogenesis (K14-HPV16 mice). We have studied the effect of genetic deletion of PAI-1 on inflammation, angiogenesis, lymphangiogenesis, as well as tumor progression. In this model, PAI-1 deficiency neither impaired keratinocyte hyperproliferation or tumor development, nor affected the infiltration of inflammatory cells and development of angiogenic or lymphangiogenic vasculature. We are reporting evidence for concomitant lymphangiogenic and angiogenic switches independent to PAI-1 status. Taken together, these data indicate that PAI-1 is not rate limiting for neoplastic progression and vascularization during premalignant progression, or that there is a functional redundancy between PAI-1 and other tumor regulators, masking the effect of PAI-1 deficiency in this long-term model of multi-stage epithelial carcinogenesis. [less ▲]

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See detailBone marrow-derived mesenchymal cells and MMP13 contribute to experimental choroidal neovascularization.
Lecomte, Julie ULg; Louis, Krystel; Detry, Benoît ULg et al

in Cellular and Molecular Life Sciences : CMLS (2011), 68

In this study, we evaluate the potential involvement of collagenase-3 (MMP13), a matrix metalloproteinase (MMP) family member, in the exudative form of age-related macular degeneration characterized by a ... [more ▼]

In this study, we evaluate the potential involvement of collagenase-3 (MMP13), a matrix metalloproteinase (MMP) family member, in the exudative form of age-related macular degeneration characterized by a neovascularisation into the choroid. RT-PCR analysis revealed that human neovascular membranes issued from patients with AMD expressed high levels of Mmp13. The contribution of MMP13 in choroidal neovascularization (CNV) formation was explored by using a murine model of laser-induced CNV and applying it to wild-type mice (WT) and Mmp13-deficient mice (Mmp13 ( -/- ) mice). Angiogenic and inflammatory reactions were explored by immunohistochemistry. The implication of bone marrow (BM)-derived cells was determined by BM engraftment into irradiated mice and by injecting mesenchymal stem cells (MSC) isolated from WT BM. The deficiency of Mmp13 impaired CNV formation which was fully restored by WT BM engraftment and partially rescued by several injections of WT MSC. The present study sheds light on a novel function of MMP13 during BM-dependent choroidal vascularization and provides evidence for a role for MSC in the pathogenesis of CNV. [less ▲]

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See detailCellules Tumorales Circulantes : détection, caractérisation et intérêts cliniques
Gilles, Christine ULg; COLLIGNON, Joëlle ULg; Noël, Agnès ULg et al

in Revue Médicale de Liège (2011), 66(5-6), 279-84

The metastatic process generates circulating tumor cells (CTCs) and disseminated tumor cells (DTCs) in bone marrow and other organs which can remain as occult metastases. Various methods and systems have ... [more ▼]

The metastatic process generates circulating tumor cells (CTCs) and disseminated tumor cells (DTCs) in bone marrow and other organs which can remain as occult metastases. Various methods and systems have been developed to allow the isolation and identification of those cells but major technical limitations still exist. Research on CTCs is a nevertheless tremendously growing field of cancer research because of their potential clinical applications. CTCs indeed convey predictive information for the development of metastasis and recurrence, and prognostic information regarding patient survival. CTCs enumeration could also be used to monitor the effectiveness of adjuvant treatments. Moreover, enhancing our basic understanding of the metastatic process, CTCs, and DTCs in particular, are thought to contain subpopulations of cells with stem cells properties that would be responsible for relapses. [less ▲]

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See detailAnalgésie épidurale obstétricale et pseudoxanthome élastique : à propos d’un cas
Clanet, Matthieu; CHANTRAINE, Frédéric ULg; DEWANDRE, Pierre-Yves ULg et al

in Annales Françaises d'Anesthésie et de Réanimation (2011), 30(9), 685-7

Pseudoxanthoma elasticum is a rare inherited disorder of the elastic tissue characterised by multisystem manifestations. Skin, eyes, gastro-intestinal system and cardiovascular system are the major ... [more ▼]

Pseudoxanthoma elasticum is a rare inherited disorder of the elastic tissue characterised by multisystem manifestations. Skin, eyes, gastro-intestinal system and cardiovascular system are the major affected systems. We describe the anaesthetic management of a parturient affected by this disease. [less ▲]

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See detailInfluence of HIV-1 and placental malaria co-infection on newborn biometry and Apgar scores in Kinshasa Democratic Republic of Congo
Modia O'Yandjo, A.; Foidart, Jean-Michel ULg; RIGO, Jacques ULg

in Journal de Gynécologie, Obstétrique et Biologie de la Reproduction (2011), 40(5), 460-4

Objective The aim of this study was to assess the impact of HIV-1 and placental malaria co-infection on newborn biometry and Apgar scores. Methods 146 HIV-1 infected and 149 HIV-1 uninfected consent ... [more ▼]

Objective The aim of this study was to assess the impact of HIV-1 and placental malaria co-infection on newborn biometry and Apgar scores. Methods 146 HIV-1 infected and 149 HIV-1 uninfected consent mothers and their newborns were recruited. Placental biopsies examination confirmed the presence or absence of placental malaria. Birth weight (BW), placental weight, cranial circumference, brachial perimeter, height, Body Mass Index (BMI) and Apgar scores at 1’, 5’, 10’ were taken. The Chi2 test and t -Student test were used for data statistical analysis. Results The global placental malarial infection prevalence was 72% but was 91% in HIV-1 infected vs. 53.7% in HIV-1 uninfected mothers (p <0.0001). The mean BW of HIV-1 co-infected mother’s newborns was slightly inferior to that of HIV-1 uninfected mother’s babies (3,033±524g vs. 3,236±565g) but this difference was not statistically significant (p >0.05). No other significant biometric differences were noted (p >0.05). But, the co-infection influenced negatively Apgar scores at 5’ (p <0.05). Conclusion HIV-1 co-infected mothers were more frequently exposed to placental malaria infection. The co-infection reduced the Apgar scores taken at the fifth minute. [less ▲]

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See detailPotential Therapeutic Target Discovery by 2D-DIGE Proteomic Analysis in Mouse Models of Asthma
QUESADA CALVO, Florence ULg; Fillet, Marianne ULg; Renaut, Jenny et al

in Journal of Proteome Research (2011), 10(9), 4291-4301

As asthma physiopathology is complex and not fully understood to date; it is expected that new key mediators are still to be unveiled in this disease. The main objective of this study was to discover ... [more ▼]

As asthma physiopathology is complex and not fully understood to date; it is expected that new key mediators are still to be unveiled in this disease. The main objective of this study was to discover potential new target proteins with a molecular weight >20 kDa by using two-dimensional differential in-gel electrophoresis (2D-DIGE) on lung parenchyma extracts from control or allergen-exposed mice (ovalbumin). Two different mouse models leading to the development of acute airway inflammation (5 days allergen exposure) and airway remodeling (10 weeks allergen exposure) were used. This experimental setting allowed the discrimination of 33 protein spots in the acute inflammation model and 31 spots in the remodeling model displaying a differential expression. Several proteins were then identified by MALDI-TOF/TOF MS. Among those differentially expressed proteins, PDIA6, GRP78, Annexin A6, hnRPA3, and Enolase display an increased expression in lung parenchyma from mice exposed to allergen for 5 days. Conversely, Apolipoprotein A1 was shown to be decreased after allergen exposure in the same model. Analysis on lung parenchyma of mice exposed to allergens for 10 weeks showed decreased calreticulin levels. Changes in the levels of those different mediators were confirmed by Western blot and immunohistochemical analysis. Interestingly, alveolar macrophages isolated from lungs in the acute inflammation model displayed enhanced levels of GRP78. Moreover, intratracheal instillation of anti-GRP78 siRNA in allergen-exposed animals led to a decrease in eosinophilic inflammation and bronchial hyperresponsiveness. This study unveils new mediators of potential importance that are up- and down-regulated in asthma. Among up-regulated mediators, GRP-78 appears as a potential new therapeutic target worthy of further investigations. [less ▲]

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See detailNew pre-conception immune biomarkers for clinical practice: interleukin-18, interleukin-15 and TWEAK on the endometrial side, G-CSF on the follicular side.
Lédée, Nathalie; Petitbarat, M.; Rahmati, M. et al

in Journal of Reproductive Immunology (2011), 88(2), 118-123

dentification of biomarkers of optimal uterine receptivity to the implanting embryo as well as biomarkers of oocyte competence would undoubtedly improve the efficiency of assisted reproductive technology ... [more ▼]

dentification of biomarkers of optimal uterine receptivity to the implanting embryo as well as biomarkers of oocyte competence would undoubtedly improve the efficiency of assisted reproductive technology (ART). Expression of IL-15 and IL-18 has been shown to be different in patients with failed implantation after IVF/ICSI compared with fertile controls and both correlate with local uNK (CD56+) recruitment and angiogenesis. Tumor necrosis factor weak inducer of apoptosis (TWEAK) has been described in mice as a potent early immune regulator able to protect the conceptus. The results of our studies in human suggest that TWEAK modulates the IL-18 related cytotoxicity of uNK cells. Quantification of IL-18, TWEAK and IL-15 mRNA expression by real-time PCR in endometrial tissue collected in mid-luteal phase of non-conception cycles allowed documentation of physiological events that occur at the time of uterine receptivity. Such information may be useful for the physician especially in patients where embryos fail to implant. Cytokine quantification may assist in understanding the mechanisms leading to repeated IVF/ICSI failure: either depletion of cytokines necessary for the apposition-adhesion, or an excess of cytokines leading to local cytotoxicity, may impair the implantation of the embryo. Other new data suggest that a pre-conception dialogue mediated by the oocyte and the follicular fluid and the oocyte may contribute to later implantation success. Follicular concentration of G-CSF appears as a useful biomarker of oocyte competence before fertilization. Moreover both in human and animal models, evidence of a role of the endometrium as a biosensor of the embryo is emerging. [less ▲]

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See detailTWEAK Appears as a Modulator of Endometrial IL-18 Related Cytotoxic Activity of Uterine Natural Killers
Petitbarat, M.; Rahmati, M.; Sérazin, V. et al

in PLoS ONE (2011), 6(1), 14497

BACKGROUND: TWEAK (Tumor necrosis factor like WEAK inducer of apoptosis) is highly expressed by different immune cells and triggers multiple cellular responses, including control of angiogenesis. Our ... [more ▼]

BACKGROUND: TWEAK (Tumor necrosis factor like WEAK inducer of apoptosis) is highly expressed by different immune cells and triggers multiple cellular responses, including control of angiogenesis. Our objective was to investigate its role in the human endometrium during the implantation window, using an ex-vivo endometrial microhistoculture model. Indeed, previous results suggested that basic TWEAK expression influences the IL-18 related uNK recruitment and local cytotoxicity. METHODOLOGY/PRINCIPAL FINDINGS: Endometrial biopsies were performed 7 to 9 days after the ovulation surge of women in monitored natural cycles. Biopsies were cut in micro-pieces and cultured on collagen sponge with appropriate medium. Morphology, functionality and cell death were analysed at different time of the culture. We used this ex vivo model to study mRNA expressions of NKp46 (a uNK cytotoxic receptor) and TGF-beta1 (protein which regulates uNK cytokine production) after adjunction of excess of recombinant IL-18 and either recombinant TWEAK or its antibody. NKp46 protein expression was also detailed by immunohistochemistry in selected patients with high basic mRNA level of IL-18 and either low or high mRNA level of TWEAK. The NKp46 immunostaining was stronger in patients with an IL-18 over-expression and a low TWEAK expression, when compared with patients with both IL-18 and TWEAK high expressions. We did not observe any difference for TWEAK expression when recombinant protein IL-18 or its antibody was added, or conversely, for IL-18 expression when TWEAK or its antibody was added in the culture medium. In a pro-inflammatory environment (obtained by an excess of IL-18), inhibition of TWEAK was able to increase significantly NKp46 and TGF-beta1 mRNA expressions. CONCLUSIONS/SIGNIFICANCE: TWEAK doesn't act on IL-18 expression but seems to control IL-18 related cytotoxicity on uNK cells when IL-18 is over-expressed. Thus, TWEAK appears as a crucial physiological modulator to prevent endometrial uNK cytotoxicity in human. [less ▲]

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See detailVascularization of the placenta and the sub-placental myometrium: feasibility and reproducibility of a three-dimensional power Doppler ultrasound quantification technique. A pilot study.
Morel, O.; Grange, G.; Fresson, J. et al

in Journal of Maternal-Fetal & Neonatal Medicine (2011), 24(2), 284-290

Objective. To assess the feasibility of placental and myometrial vascularization quantification using 3D power Doppler ultrasonography. Methods. 3D standardized acquisition was performed in the mid part ... [more ▼]

Objective. To assess the feasibility of placental and myometrial vascularization quantification using 3D power Doppler ultrasonography. Methods. 3D standardized acquisition was performed in the mid part of the utero-placental unit, once, in 38 patients undergoing normal pregnancies between 15 and 39 weeks. Vascularization parameters (VI, FI, and VFI) of placentae and myometrium were measured. Intra and inter-observer, as well as inter-acquisition reproducibility were evaluated. Results. Intra-class Correlation Coefficient of vascularization measurements were at least 0.94 for intra-observer, 0.92 for inter-observer, and 0.56 for inter-acquisition reproducibility. There was no significant difference for placental measurements for VI, FI and VFI between the second trimester and the third trimester pregnancies. Concerning the myometrium, we observed no significant difference between second and third trimester for FI. However, VI (28.090 vs. 19.374) and VFI (17.691 vs. 11.336) was significantly lower in the third trimester (p < 0.01). Conclusion. 3D quantification of placental and myometrial vascular parameters is feasible with a high intra and inter-observer reproducibility. Evaluating a potential myometrial vascular impairment appears to be as relevant as studying the placenta alone and might be of great clinical interest. We believe that this technique should therefore be evaluated in clinical observational studies. [less ▲]

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See detailIs the baboon model appropriate for endometriosis studies?
Dehoux, Jean-Paul; Defrère, Silvie; Squifflet, Jean et al

in Fertility and Sterility (2011), 96(3), 728-7333

Objective To determinethe prevalence of spontaneous endometriosis andthe incidence of induced endometriosis after endocervical canal resection in baboons. Design Induction and follow-up of endometriosis ... [more ▼]

Objective To determinethe prevalence of spontaneous endometriosis andthe incidence of induced endometriosis after endocervical canal resection in baboons. Design Induction and follow-up of endometriosis in baboons, which is one of the primate species that develop spontaneous endometriosis. Forty-one baboons were checked for the presence of spontaneous endometriosis. We then attempted to induce endometriosis in 30 of them by endocervical canal resection. Setting Institute of Primate Research, Nairobi, Kenya, and Catholic University of Louvain, Brussels, Belgium. Animal(s) Forty-one baboons were checked for spontaneous endometriosis and 30 of them were used to develop a model of induced endometriosis. Intervention(s) A total of 41 baboons underwent diagnostic laparoscopy for 10 months. In a first step, 30 of this number subsequently underwent endocervical canal resection. In a second step, 20 of the 30 underwent uterine horn resection. Main Outcome Measure(s) Follow-up by laparoscopy. Result(s) Two of the 41 baboons were diagnosed with spontaneous endometriosis (4.8%). Twelve months after the surgical procedure to induce endometriosis, 8 of 29 animals presented with endometriotic lesions diagnosed by using laparoscopy and confirmed by histologic examination. The incidence of induced endometriosis in our model was thus 27.6%. In 2 baboons, endometriosis disappeared over time, resulting in a final rate of 20.7% (6/29). Conclusion(s) The rate of spontaneous endometriosis is very low (4.8%). Endometriosis can be induced (with a rate of just 27.6%) by endocervical canal resection to stimulate retrograde menstruation. [less ▲]

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See detailSpecific and extensive endometrial deregulation is present before conception in IVF/ICSI repeated implantation failures (IF) or recurrent miscarriages.
Lédée, Nathalie; Munaut, Carine ULg; Aubert, Julie et al

in Journal of Pathology (The) (2011), 225(4), 554-64

The objective was to examine if IVF/ICSI repeated implantation failures (IF) or recurrent miscarriages (RM) could be related to preconceptional endometrial deregulations. IF was defined as the absence of ... [more ▼]

The objective was to examine if IVF/ICSI repeated implantation failures (IF) or recurrent miscarriages (RM) could be related to preconceptional endometrial deregulations. IF was defined as the absence of pregnancy despite the transfer of at least ten IVF/ICSI good quality embryos, and RM as having at least three unexplained miscarriages. Fertile controls (FC) were women who had given birth at least once. Endometrial biopsy was performed in the mild luteal phase of a non-conceptual cycle (five women were selected in each group). Affymetrix chips (GeneChip Human Genome U133 Plus2.0 Array) were used for hybridization. Data were normalized by the gcRMA method, and raw p values adjusted by the Bonferroni procedure (1%). Differential expression of selected genes was analysed using real-time PCR. Gene networks and biological functions were explored using the Ingenuity Pathways Analysis software. Endometrial gene expression profiles at the time of uterine receptivity differ dramatically in the endometrium among FC, RM, and IF patients. Compared to FC, 2126 and 2477 genes are differentially expressed in IF and RM groups, respectively, and 2363 between IF and RM. In both conditions, differential gene expression referred mainly to DNA transcription and expression. Other main cellular functions deregulated in IF conditions correspond to cell morphology, cellular development, cell cycle, and cellular assembly, while in RM conditions, deregulated cellular functions relate to cell signalling (degradation of cyclic AMP and calcium metabolism) and cellular maintenance. In both conditions, there is an over-representation of deregulations related to the haematological system. In the IF condition, cell-mediated immune response and nervous system development and function are highly deregulated, while in RM patients, main deregulations are in organ and tissue development, humoral immune response, and muscular system development and function. Extensive endometrial deregulations are present before conception in patients who experienced IF or RM with both distinct and common deregulation. [less ▲]

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See detailAn insight into normal and pathological pregnancies using large-scale microarrays: lessons from microarrays
Chaouat, G. R.; Rodde, N.; Petitbarat, M. et al

in Journal of Reproductive Immunology (2011), 89(2), 163-72

In the introduction, we briefly recall old but classic evidence that there is no tolerance to paternal alloantigens in a first pregnancy. Therefore, we performed small- and large-scale microarrays in CBA ... [more ▼]

In the introduction, we briefly recall old but classic evidence that there is no tolerance to paternal alloantigens in a first pregnancy. Therefore, we performed small- and large-scale microarrays in CBA × DBA/2 and CBA × BALB/c combinations, recently described as a murine model for preeclampsia. Our results are in line with other data suggesting a very early deregulation of local immune vascular events rather than a break of immune tolerance. Other data presented at the Tioman 2010 Preeclampsia Workshop supporting this hypothesis are briefly summarised, as well as indications and caveats from a recent human microarray on implantation failure and recurrent pregnancy loss. [less ▲]

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See detailAnalysis of fetal death in relation with increased fetal nuchal translucency thickness in the south of Vietnam
To, Hong ULg; SCHAAPS, Jean-Pierre ULg; FOIDART, Jean-Michel ULg

in Nobuyuki, Taniguichi (Ed.) Asian Federation of Societies for Ultrasound in Medicine and Biology: AFSUMB, New Delhi 18-21 november 2010 (2010, November 18)

Objectives: The aim of this study was to investigate the fetal deaths relation with an increased fetal nuchal translucency (NT) thickness in the South of Vietnam. Methods: A total of 2500 singleton ... [more ▼]

Objectives: The aim of this study was to investigate the fetal deaths relation with an increased fetal nuchal translucency (NT) thickness in the South of Vietnam. Methods: A total of 2500 singleton pregnancies were measured fetal NT thickness and performed free beta subunit of human Chorionic Gonadotropin (free β-hCG) and pregnancy associated plasma protein-A (PAPP-A) routinely in the first trimester, then scanned systematically for fetal structure by ultrasonography at the second trimester of gestation, next followed to their delivery and examined neonatal status. For suspicion of fetal abnormality, amniocentesis was indicated to confirm a fetal karyotype. Fetal abnormal chromosome was counselled a termination of pregnancy (TOP). 5 groups of fetal NT thickness was divided: group of normal fetal NT thickness (< 2.4mm), group of mild increased of fetal NT thickness (2.4-3.4mm), group of moderate increased fetal NT (3.5-4.4mm), group of severe fetal NT (4.5-5.4mm) and group of very severe fetal NT (≥ 5.5mm). We evaluated the fetal deaths (included spontaneous abortion, fetal death intra-uterine, and terminated pregnancy due to aneuploidy and/or structural abnormality), then look for a relation between an increased fetal NT thickness and those fetal adverse outcomes. Results: Fetal NT thickness cut-off point at 2.4mm or more was defined as an increased fetal NT thickness with 65.5% of sensitivity for fetal abnormal detection. 5.3% (133/2500) was the prevalence of increased fetal NT thickness. 2.8% (71/2500) of cases had been indicated an amniocentesis. A total incidence of fetal death was found 1.5% (37/2500). In group of fetal NT thickness < 2.4mm, the rate of abnormal karyotype was 0.4% (10/2367), none case of fetal deaths obtained. In the fetal NT group of 2.4-3.4mm, total of fetal death found 26.4% (32/121) that included 12.4% (15/121) of abnormal karyotype, 8.3% (10/121) of abnormal ultrasound scanning, 5.8% (7/121) of fetal demised, and 0.8% (1 case) of neonatal defect due to G6PD deficiency. In the group of 3.5-4.4mm, 33.3% (3/9) of fetuses were died (22.2% was due to aneuploidy and 11.1% was due to miscarriage spontaneously). In the group of 4.5-5.4mm, 1 case (50%) of trisomy 18 was detected and in the group of ≥ 5.5mm, 1 case (100%) of trisomy 21 had been diagnosed. When fetal NT thickness increased, the possibility of fetal demised was higher significantly: likelihood ratio (LR) augmented from 15.2 in the fetal NT group at 2.4-3.4mm to 169.5 in fetal NT group at ≥ 5.5mm (p < 0.01, Pearson chi2). Conclusion: An increased fetal NT thickness was useful finding for prediction of fetal deaths (included fetal abortion, demised or terminated) in the prenatal diagnosis and care program in the South of Vietnam. [less ▲]

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See detailAnalysis of fetal death in relation with increased fetal nuchal translucency thickness in the south of Vietnam
To, Hong ULg; Schaaps, Jean-Pierre ULg; Foidart, Jean-Michel ULg

in Asian Federation of Societies for Ultrasound in Medicine and Biology (2010, November 18)

Objectives: The aim of this study was to investigate the fetal deaths relation with an increased fetal nuchal translucency (NT) thickness in the South of Vietnam. Methods: A total of 2500 singleton ... [more ▼]

Objectives: The aim of this study was to investigate the fetal deaths relation with an increased fetal nuchal translucency (NT) thickness in the South of Vietnam. Methods: A total of 2500 singleton pregnancies were measured fetal NT thickness and performed free beta subunit of human Chorionic Gonadotropin (free β-hCG) and pregnancy associated plasma protein-A (PAPP-A) routinely in the first trimester, then scanned systematically for fetal structure by ultrasonography at the second trimester of gestation, next followed to their delivery and examined neonatal status. For suspicion of fetal abnormality, amniocentesis was indicated to confirm a fetal karyotype. Fetal abnormal chromosome was counselled a termination of pregnancy (TOP). 5 groups of fetal NT thickness was divided: group of normal fetal NT thickness (< 2.4mm), group of mild increased of fetal NT thickness (2.4-3.4mm), group of moderate increased fetal NT (3.5-4.4mm), group of severe fetal NT (4.5-5.4mm) and group of very severe fetal NT (≥ 5.5mm). We evaluated the fetal deaths (included spontaneous abortion, fetal death intra-uterine, and terminated pregnancy due to aneuploidy and/or structural abnormality), then look for a relation between an increased fetal NT thickness and those fetal adverse outcomes. Results: Fetal NT thickness cut-off point at 2.4mm or more was defined as an increased fetal NT thickness with 65.5% of sensitivity for fetal abnormal detection. 5.3% (133/2500) was the prevalence of increased fetal NT thickness. 2.8% (71/2500) of cases had been indicated an amniocentesis. A total incidence of fetal death was found 1.5% (37/2500). In group of fetal NT thickness < 2.4mm, the rate of abnormal karyotype was 0.4% (10/2367), none case of fetal deaths obtained. In the fetal NT group of 2.4-3.4mm, total of fetal death found 26.4% (32/121) that included 12.4% (15/121) of abnormal karyotype, 8.3% (10/121) of abnormal ultrasound scanning, 5.8% (7/121) of fetal demised, and 0.8% (1 case) of neonatal defect due to G6PD deficiency. In the group of 3.5-4.4mm, 33.3% (3/9) of fetuses were died (22.2% was due to aneuploidy and 11.1% was due to miscarriage spontaneously). In the group of 4.5-5.4mm, 1 case (50%) of trisomy 18 was detected and in the group of ≥ 5.5mm, 1 case (100%) of trisomy 21 had been diagnosed. When fetal NT thickness increased, the possibility of fetal demised was higher significantly: likelihood ratio (LR) augmented from 15.2 in the fetal NT group at 2.4-3.4mm to 169.5 in fetal NT group at ≥ 5.5mm (p < 0.01, Pearson chi2). Conclusion: An increased fetal NT thickness was useful finding for prediction of fetal deaths (included fetal abortion, demised or terminated) in the prenatal diagnosis and care program in the South of Vietnam. [less ▲]

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See detailhCG: a pregnancy-related hormone stimulating angiogenesis and pericyte recruitment
Berndt, S; Blacher, Silvia ULg; Perrier d’Hauterive, S et al

Poster (2010)

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See detailRole of delta-like-4/Notch in the formation and wiring of the lymphatic network in zebrafish.
Geudens, I.; Herpers, R.; Hermans, K. et al

in Arteriosclerosis, Thrombosis, and Vascular Biology (2010), 30(9), 1695-702

OBJECTIVE: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development. METHODS AND RESULTS: In zebrafish embryos, sprouts from the axial ... [more ▼]

OBJECTIVE: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development. METHODS AND RESULTS: In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates. CONCLUSIONS: These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network. [less ▲]

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See detailAetiology and physiopathology of preeclampsia and related forms.
Lorquet, Sophie ULg; Pequeux, Christel ULg; Munaut, Carine ULg et al

in Acta Clinica Belgica (2010), 65(4), 237-41

Preeclampsia, a pregnancy-specific syndrome characterized by hypertension, proteinuria and oedema, resolves on placental delivery. Its pathogenesis is thought to be associated to a hypoxic placenta ... [more ▼]

Preeclampsia, a pregnancy-specific syndrome characterized by hypertension, proteinuria and oedema, resolves on placental delivery. Its pathogenesis is thought to be associated to a hypoxic placenta. Placental hypoxia is responsible for the maternal vascular dysfunction via the increased placental release of anti-angiogenic factors such as soluble flt1 and endoglin. These soluble receptors bind VEGF, PLGF and TGFbeta1 and 3 in the maternal circulation, causing endothelial dysfunction in many maternal tissues. Despite these recent and important new molecular findings, it is important to consider that normal pregnancy is also characterized by systemic inflammation, oxidative stress and alterations in levels of angiogenic factors and vascular reactivity. Both the placenta and maternal vasculatures are major sources of reactive oxygen and nitrogen species which can produce powerful pro-oxidants that covalently modify proteins and alter vascular function in preeclampsia. Finally, the recent demonstration of activating auto-antibodies to the Angiotensin 1 receptor that experimentally play a major pathogenic role in preeclampsia further indicates the pleiotropism of aetiologies of this condition. [less ▲]

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