References of "Evrard, Brigitte"
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See detailDevelopment and validation of a new Fourier transform infrared spectrometric method for the quantification of urea in creams and ointments
Otte, X; Evrard, Brigitte ULg; Delattre, Luc ULg et al

in Analytica Chimica Acta (2002), 451

A new Fourier transform infrared (FTIR) spectrometric method for the quantification of urea in creams and ointments was developed and validated. The sample was solubilized in a mixture of chloroform ... [more ▼]

A new Fourier transform infrared (FTIR) spectrometric method for the quantification of urea in creams and ointments was developed and validated. The sample was solubilized in a mixture of chloroform/acetonitrile (1/1 v/v) which allows to solubilize the vaseline or other fats as well as urea and water. The solution was examined in a transmission cell with a pathlength of 500 μm provided with NaCI windows. The urea spectrum was obtained by subtraction of the reference solvent mixture spectrum and the reference water spectrum from the sample spectrum. The absorbance of the peak at ±1688 cm-1 was compared to those of calibration standards to quantify urea. The detection limit and the quantification limit (three times and 10 times the noise, respectively) were estimated as 0.4 and 1.3 μg ml-1, respectively. Validation of the method was realized and application of this method to stability tests was done. [less ▲]

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See detailIn Vitro Comparison of the Antimycotic Activity of a Miconazole-Hp-Beta-Cyclodextrin Solution with a Miconazole Surfactant Solution
Piel, Géraldine ULg; Hayette, Marie-Pierre ULg; Pavoni, Ermanno et al

in Journal of Antimicrobial Chemotherapy (2001), 48(1), 83-7

The antimycotic activity of a new parenteral solution containing miconazole was compared with that of a marketed solution (Daktarin IV solution). This solution has been withdrawn from the Belgian market ... [more ▼]

The antimycotic activity of a new parenteral solution containing miconazole was compared with that of a marketed solution (Daktarin IV solution). This solution has been withdrawn from the Belgian market, probably because of toxic effects related to the presence of polyoxyl 35 castor oil. We propose a new formulation containing miconazole (10 mg/mL) (like the marketed solution), in combination with hydroxypropyl-beta-cyclodextrin and lactic acid. The MICs of these two solutions were determined by a broth microdilution method (based on NCCLS guidelines) for 67 yeasts and 50 filamentous fungi isolates. This study shows that the MICs obtained with these two solutions are not significantly different. [less ▲]

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See detailMolecular Modeling Study of Beta- and Gamma-Cyclodextrin Complexes with Miconazole
Piel, Géraldine ULg; Dive, Georges ULg; Evrard, Brigitte ULg et al

in European Journal of Pharmaceutical Sciences (2001), 13(3), 271-9

Different authors have demonstrated the inclusion of miconazole in cyclodextrins (CD). Miconazole can be included in the CD cavity both in the neutral and in the ionized form. The present study tries to ... [more ▼]

Different authors have demonstrated the inclusion of miconazole in cyclodextrins (CD). Miconazole can be included in the CD cavity both in the neutral and in the ionized form. The present study tries to understand which fragment of the miconazole molecule is involved in the inclusion. Austin Model 1 approximate molecular orbital calculations have been performed on several complexes between beta-cyclodextrin (betaCD) or gamma-cyclodextrin (gammaCD) and miconazole in the ionized and the non-ionized forms of the two R and S enantiomers in three different orientations. We observed that betaCD is a good vehicle to transport miconazole which can be very easily released. The complexation energy between miconazole and betaCD is not very high but the entropic factor has a great incidence on the stability of the formed complex. The inclusion of the dichlorobenzene-CH(2)-O- and of the imidazole part of the S isomer gives rise to the most probable complex in acidic conditions (ionized miconazole). Nevertheless, the inclusion should be considered as a dynamic process in which different parts of the molecule could be alternatively included in betaCD. The present work demonstrates the high capability of deformation of betaCD which could easily accommodate several types of ligand. By opposite, the cycle extension in gammaCD leads to a more rigid vehicle with regards to miconazole. [less ▲]

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See detailFormulation and characterisation of submicronic emulsions
Roland, Isabelle ULg; Evrard, Brigitte ULg; Erpicum, Thomas et al

Conference (2001)

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See detailFormulation of subcronic emulsions by using a high-pressure homogeniser
Roland, Isabelle ULg; Evrard, Brigitte ULg; Erpicum, Thomas et al

Conference (2001)

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See detailPreparation of mebendazole HP-beta-cyclodextrin complexes using water-soluble polymers and organic acids
Alvarez, Covadonga; Van Hees, Thierry ULg; Piel, Géraldine ULg et al

in STP Pharma Sciences (2001), 11(6), 439-442

Mebendazole (MBZ) is a carbamate derivative with an aminobenzimidazole structure. It possesses antihelminthic properties and is particularly insoluble in water. In order to increase its aqueous solubility ... [more ▼]

Mebendazole (MBZ) is a carbamate derivative with an aminobenzimidazole structure. It possesses antihelminthic properties and is particularly insoluble in water. In order to increase its aqueous solubility, hydroxpropyl-beta-cyclodextrin (HP-beta-CD) was used in combination with water-soluble polymers (polyvinylpyrrolidone or hydroxypropymethyl cellulose) and different organic acids (citric or tartaric). Increased solubility was observed on heating the mixture. The effects of the time and temperature of the heating process on drug solubility and stability were investigated. The results clearly show that the best conditions for increasing solubility and limiting degradation of MBZ is to heat a combination of MBZ, HP-beta-CD (200 mM), tartaric or citric acid (50 mM) and HPMC (0.1% w/v) in a water-bath at 95degreesC for 60 min. This method increases solubility to about 680 mug/ml and limits degradation to 2.5%. [less ▲]

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See detailPréparation de complexes mebendazole-HPβCD à l'aide de polymères solubles dans l'eau et d'acides organiques
Alvarez, C; Van Hees, Thierry ULg; Piel, Géraldine ULg et al

in Annales Pharmaceutiques Françaises (2001), 59

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See detailPreparation of mebendazole HP-b-cyclodextrin complexes using water soluble polymers and organic acids
Alvarez, Covadonga; Van Hees, Thierry ULg; Piel, Géraldine ULg et al

Conference (2000, April)

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See detailDetermination of Albendazole and Its Main Metabolites in Ovine Plasma by Liquid Chromatography with Dialysis as an Integrated Sample Preparation Technique
Chiap, Patrice ULg; Evrard, Brigitte ULg; Bimazubute, M. A. et al

in Journal of Chromatography. A (2000), 870(1-2), 121-34

Albendazole is a benzimidazole derivative with a broad-spectrum activity against human and animal helminth parasites. In order to determine the main pharmacokinetic parameters in sheep after oral and ... [more ▼]

Albendazole is a benzimidazole derivative with a broad-spectrum activity against human and animal helminth parasites. In order to determine the main pharmacokinetic parameters in sheep after oral and intravenous administration of a new formulation of albendazole (an aqueous solution), a fully automated method was developed for the determination of this drug and its main metabolites, albendazole sulfoxide (active metabolite) and sulfone in ovine plasma. This method involves dialysis as purification step, followed by enrichment of the dialysate on a precolumn and liquid chromatography (LC). All sample handling operations were executed automatically by means of an ASTED XL system. After conditioning of the trace enrichment column (TEC) packed with octadecyl silica with pH 6.0 phosphate buffer containing sodium azide, the plasma sample, in which a protein releasing reagent (1 M HCl) containing Triton X-100 was automatically added, was loaded in the donor channel and dialysed on a cellulose acetate membrane in the static-pulsed mode. The dialysis liquid consisted of pH 2.5 phosphate buffer. By rotation of a switching valve, the analytes were eluted from the TEC in the back-flush mode by the LC mobile phase and transferred to the analytical column, packed with octyl silica. The chromatographic separation was performed at 35 degrees C and the analytes were monitored photometrically at 295 nm. Due to the differences in hydrophobic character between albendazole and its metabolites, a gradient elution was applied. The mobile phase consisted of a mixture of acetonitrile and pH 6.0 phosphate buffer. The proportion of organic modifier was increased from 10.0 to 50.1% in 12.30 min, then from 50.1 to 66.9% in 1.70 min. First, the gradient conditions and the temperature were optimised for the LC separation using the DryLab software. Then, the influence of some parameters of the dialysis process on analyte recovery was investigated. Finally, the method developed was validated. The mean recoveries for albendazole and its metabolites were about 70 and 65%, respectively. The limits of quantification for albendazole and its metabolites were 10 and 7.5 ng/ml, respectively. [less ▲]

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See detailInclusion of Piroxicam into Beta-Cyclodextrin by Means of Supercritical Carbon Dioxide: Thermal, Spectroscopic and Physicochemical Studies
Van Hees, Thierry ULg; Evrard, Brigitte ULg; Piel, Géraldine ULg et al

in Journal de Pharmacie de Belgique (2000), 55(1, Jan-Feb), 30-1

The preparation of a piroxicam-beta-cyclodextrin inclusion compound using supercritical CO2 was investigated. The solubility piroxicam in supercritical CO2 was determined. The influence of the temperature ... [more ▼]

The preparation of a piroxicam-beta-cyclodextrin inclusion compound using supercritical CO2 was investigated. The solubility piroxicam in supercritical CO2 was determined. The influence of the temperature, the pressure and the time of exposure on the inclusion rate were studied and a complete inclusion was achieved by keeping a physical mixture of piroxicam and beta-cyclodextrin (1:2.5 mol/mol) for 6 hours at 150 degrees C and 150 bar of CO2. This complex was characterised by Differential Scanning Calorimetry and Fourier Transform Infrared Spectrometry. Supercritical carbon dioxide may prove to be a novel useful complexation method of non-polar drugs into beta-cyclodextrin. [less ▲]

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See detailApplication of Supercritical Carbon Dioxide for the Preparation of a Piroxicam-Beta-Cyclodextrin Inclusion Compound
Van Hees, Thierry ULg; Piel, Géraldine ULg; Evrard, Brigitte ULg et al

in Pharmaceutical Research (1999), 16(12), 1864-70

PURPOSE: Piroxicam is a poorly soluble NSAID, whose solubility is enhanced when included into beta-cyclodextrin. The preparation of a piroxicam-beta-cyclodextrin inclusion compound using supercritical CO2 ... [more ▼]

PURPOSE: Piroxicam is a poorly soluble NSAID, whose solubility is enhanced when included into beta-cyclodextrin. The preparation of a piroxicam-beta-cyclodextrin inclusion compound using supercritical CO2 was investigated. METHODS: The solubility and the stability of piroxicam in supercritical CO2 were determined. Then, the influence of the temperature, the pressure and the time of exposure on the inclusion rate was studied. RESULTS: The solubility of piroxicam varied over a wide range depending on the temperature and pressure (from 0.006 to 1.500 mg/g of CO2). The temperature and the time of exposure had a great influence on the inclusion yield, while pressure did not and a complete inclusion was achieved by keeping a physical mixture of piroxicam and beta-cyclodextrin (1:2.5 mol/mol) for 6 hours at 150 degrees C and 15 MPa of CO2. This complex was characterized by Differential Scanning Calorimetry, differential solubility and Fourier Transform Infrared Spectrometry. CONCLUSIONS: Supercritical carbon dioxide may prove to be a novel useful complexation method of drugs into beta-cyclodextrin. [less ▲]

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See detailInfluence of Melting and Rheological Properties of Fatty Binders on the Melt Granulation Process in a High-Shear Mixer
Evrard, Brigitte ULg; Amighi, K.; Beten, D. et al

in Drug Development & Industrial Pharmacy (1999), 25(11), 1177-84

The preparation of granules by melt granulation was investigated using a laboratory-scale high-shear mixer (Pellmix PL 1/8) and binary mixtures containing lactose and different lipidic binders, namely ... [more ▼]

The preparation of granules by melt granulation was investigated using a laboratory-scale high-shear mixer (Pellmix PL 1/8) and binary mixtures containing lactose and different lipidic binders, namely, Compritol 888, Cutina HR, or Precirol ATO5. [less ▲]

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