References of "Evrard, Brigitte"
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See detailDevelopment of a new topical system: Drug-in-cyclodextrin-in-deformable liposome
Gillet, Aline ULg; Grammenos, Angeliki ULg; Compère, Philippe ULg et al

in International Journal of Pharmaceutics (2009), 380(1-2), 174-180

A new delivery system for cutaneous administration combining the advantages of cyclodextrin inclusion complexes and those of deformable liposomes was developed, leading to a new concept: drug ... [more ▼]

A new delivery system for cutaneous administration combining the advantages of cyclodextrin inclusion complexes and those of deformable liposomes was developed, leading to a new concept: drug-incyclodextrin-in-deformable liposomes. Deformable liposomes made of soybean phosphatidylcholine (PC) or dimyristoylphosphatidylcholine (DMPC) and sodium deoxycholate as edge activator were compared to classical non-deformable liposomes. Liposomes were prepared by the film evaporation method. Betamethasone, chosen as the model drug,was encapsulated in the aqueous cavity of liposomes by the use of cyclodextrins. Cyclodextrins allowan increase in the aqueous solubility of betamethasone and thus, the encapsulation efficiency in liposome vesicles. Liposome size, deformability and encapsulation efficiency were calculated. The best results were obtained with deformable liposomes made of PC in comparison with DMPC. The stability of PC vesicles was evaluated by measuring the leakage of encapsulated calcein on the one hand and the leakage of encapsulated betamethasone on the other hand. In vitro diffusion studies were carried out on Franz type diffusion cells through polycarbonate membranes. In comparison with non-deformable liposomes, these new vesicles showed improved encapsulation efficiency, good stability and higher in vitro diffusion percentages of encapsulated drug. They are therefore promising for future use in ex vivo and in vivo experiments. [less ▲]

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See detailProduction line real time near infrared (NIR) monitoring
Mantanus, Jérôme ULg; Ziemons, Eric ULg; Streel, Bruno et al

Conference (2009, September)

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See detailPreparations and characterisations of semi-solid formulations containing a hydrophilic drug for vaginal administration
Coia, Isabelle ULg; Evrard, Brigitte ULg; Piette, Marie ULg

Poster (2009, April 01)

The purpose of this study is to demonstrate the effect of different types of semi-solid formulations (aqueous gels, liquid jellified emulsions and hydrophilic or lipophilic creams) for vaginal ... [more ▼]

The purpose of this study is to demonstrate the effect of different types of semi-solid formulations (aqueous gels, liquid jellified emulsions and hydrophilic or lipophilic creams) for vaginal administration on the release kinetic of a hydrophilic drug. This drug is an acidic and hydrophilic (log P (octanol/water) = -3.3) molecule with an aqueous solubility upper than 170mg/ml at pH 6-8. The formulations characterisations consisted in the in vitro evaluation of the drug release kinetic and the measure of all formulations viscosity as well as the creams stability and emulsions droplets size. [less ▲]

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See detailDéveloppement d'un implant intra-péritonéal contre l'endométriose
Krier, Fabrice ULg; Evrard, Brigitte ULg; Piel, Géraldine ULg et al

Scientific conference (2009, February 03)

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See detailStudy of the cholesterol extraction capacity of β-cyclodextrin and its derivatives, relationships with their effects on endothelial cell viability and on membrane models
Castagne, Delphine ULg; Fillet, Marianne ULg; Delattre, Luc ULg et al

in Journal of Inclusion Phenomena and Macrocyclic Chemistry (2009), 63(3-4), 225-231

Endothelial cells (HUVEC) were treated with β-cyclodextrin and hydroxypropylated or methylated derivatives solutions in order to quantify their cholesterol extraction capacity. Non-toxic concentrations of ... [more ▼]

Endothelial cells (HUVEC) were treated with β-cyclodextrin and hydroxypropylated or methylated derivatives solutions in order to quantify their cholesterol extraction capacity. Non-toxic concentrations of cyclodextrins (CDs) were determined following methyl thiazol tetrazolium (MTT) assays, total protein measurements, morphological observations and trypan blue assays. The residual cholesterol content of cells was measured and the extraction power of CDs compared to results obtained by phase solubility diagrams. Cholesterol was extracted with a dose-response relationship, the lowest residual cholesterol content being obtained with β-CD at 10 mM. Low substituted derivatives (Crysmeb® and hydroxypropyl-β-CD) maintained liposomes integrity (as shown before), were the less cytotoxic and presented the lowest affinity for cholesterol contrary to methylated derivatives with degrees of substitution around 2. [less ▲]

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See detailMoisture content determination of pharmaceutical pellets by near infrared spectroscopy: method development and validation.
Mantanus, Jérôme ULg; Ziemons, Eric ULg; Lebrun, Pierre ULg et al

in Analytica Chimica Acta (2009), 642(1-2), 186-92

The aim of the present study was to develop and validate a near infrared method able to accurately determine a moisture content of pharmaceutical pellets ranging from 1% to 8% in order to check their ... [more ▼]

The aim of the present study was to develop and validate a near infrared method able to accurately determine a moisture content of pharmaceutical pellets ranging from 1% to 8% in order to check their moisture content conformity. A calibration and validation set were designed for the conception and evaluation of the method adequacy. An experimental protocol was then followed, involving two operators, independent production campaign batches and different temperatures for data acquisition. On the basis of this protocol, prediction models based on partial least squares (PLS) regression were then carried out. Conventional criteria such as the R(2), the root mean square errors of calibration and prediction (RMSEC and RMSEP) as well as the number of PLS factors enabled the selection of three preliminary models. However, such criteria did not clearly demonstrate the model's ability to give accurate predictions over the whole analyzed water content range. Consequently, a novel approach based on accuracy profiles which allow the selection of the most fitted model for purpose was used. According to this novel approach, the model using multiplicative scatter correction (MSC) pre-treatment was obviously the most suitable. Indeed, the resulting accuracy profile clearly showed that this model was able to determine moisture content over the range of 1-8% with a very acceptable accuracy. The present study confirmed that NIR spectroscopy could be used in the PAT concept as a non-invasive, non-destructive and fast technique for moisture content determination in pharmaceutical pellets. In addition, facing the limit of the classical and commonly used criteria, the use of accuracy profiles proved to be useful as a powerful decision tool to demonstrate the suitability of the proposed analytical method. [less ▲]

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