References of "Evrard, Brigitte"
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See detailPolymeric Nanoparticles as siRNA Drug Delivery System for Cancer Therapy: The Long Road to Therapeutic Efficiency
Frère, Antoine ULg; Evrard, Brigitte ULg; Mottet, Denis ULg et al

in Holban, Alina Maria; Grumezescu, Alexandru (Eds.) Nanoarchitectonics for Smart Delivery and Drug Targeting (2016)

Polyplexes are nanoparticles composed of small-interfering RNA (siRNA) and natural or synthetic polymers. To meet the challenge of gene therapy and deliver siRNA into the cytoplasm of target cells ... [more ▼]

Polyplexes are nanoparticles composed of small-interfering RNA (siRNA) and natural or synthetic polymers. To meet the challenge of gene therapy and deliver siRNA into the cytoplasm of target cells, several barriers must be overcome. In this chapter, the main steps, from the formulation of polyplexes to the efficient release of the siRNA into the cytoplasm of cancer cells, are described, taking into account the different strategies used to overcome the obstacles linked to the formulation of this type of nanovector. To allow a parenteral administration of the nanocolloids, the polyplex production methods should result in identical, stable, and reproducible nanostructures. Charge interactions occur between the anionic siRNA and the cationic/amphiphilic polymer. Once in the blood circulation, polyplexes must keep their physical stability. The positively charged surface can cause aggregation of the nanoparticles with plasma proteins, as well as complement activation and recognition by the mononuclear phagocytic system, with a consequent reduction of their pharmacological activity. Polyethylene glycol (PEG) can be added on the surface of the nanovectors to confer “the stealth” properties and increase plasma half-life. Then, particles have to preferentially accumulate in the tumor tissue following an active or passive targeting. Endocytosis process enables the polyplex cellular uptake, but some strategies like “the proton sponge effect” have to be used to allow the escape of the nanovectors from the cellular endosomes. Once released into the cytoplasm, polymer and siRNA must dissociate for an effective degradation of the targeted mRNA, leading finally to a decrease of the corresponding protein. [less ▲]

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See detailA simple calibration approach based on film-casting for confocal Raman microscopy to support the development of a Hot-Melt Extrusion process
Netchacovitch, Lauranne ULg; Thiry, Justine ULg; De Bleye, Charlotte ULg et al

in Talanta (2016), 154

When developing a new formulation, the development, calibration and validation steps of analytical methods based on vibrational spectroscopy are time-consuming. For each new formulation, real samples must ... [more ▼]

When developing a new formulation, the development, calibration and validation steps of analytical methods based on vibrational spectroscopy are time-consuming. For each new formulation, real samples must be produced and a “reference method” must be used in order to determine the Active Pharmaceutical Ingredient (API) content of each sample. To circumvent this issue, the paper presents a simple approach based on the film-casting technique used as a calibration tool in the framework of hot-melt extrusion process. Confocal Raman microscopic method was successfully validated for the determination of itraconazole content in film-casting samples. Then, hot-melt extrusion was carried out to produce real samples in order to confront the results obtained with confocal Raman microscopy and Ultra High Performance Liquid Chromatography (UHPLC). The agreement between both methods was demonstrated using a comparison study based on the Bland and Altman’s plot. [less ▲]

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See detailFreeze-dried mucoadhesive polymeric system containing pegylated lipoplexes: Towards a vaginal sustained released system for siRNA
Furst, Tania ULg; Dakwar, George R.; Zagato, Elisa et al

in Journal of Controlled Release (2016)

Topical vaginal sustained delivery of siRNA presents a significant challenge due to the short residence time of formulations. Therefore, a drug delivery system capable to adhere to the vaginal mucosa is ... [more ▼]

Topical vaginal sustained delivery of siRNA presents a significant challenge due to the short residence time of formulations. Therefore, a drug delivery system capable to adhere to the vaginal mucosa is desirable, as it could allow a prolonged delivery and increase the effectiveness of the therapy. The aim of this project is to develop a polymeric solid mucoadhesive system, loaded with lipoplexes, able to be progressively rehydrated by the vaginal fluids to form a hydrogel and to deliver siRNA to vaginal tissues. To minimize adhesive interactions with vaginal mucus components, lipoplexes were coated with different derivatives of polyethylene glycol: DPSE-PEG2000, DPSE-PEG750 and ceramide-PEG2000. Based on stability and diffusion properties in simulated vaginal fluids, lipoplexes containing DSPE-PEG2000 were selected and incorporated in hydroxyethyl cellulose (HEC) hydrogels. Solid systems, called sponges, were then obtained by freeze-drying. Sponges meet acceptable mechanical characteristics and their hardness, eformability and mucoadhesive properties are not influenced by the presence of lipoplexes. Finally, mobility and stability of lipoplexes inside sponges rehydrated with vaginal mucus, mimicking in situ conditions, were evaluated by advanced fluorescence microscopy. The release rate was found to be influenced by the HEC concentration and consequently by the viscosity after rehydration. This study demonstrates the feasibility of entrapping pegylated lipoplexes into a solid matrix system for a prolonged delivery of siRNA into the vagina. [less ▲]

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See detailModeling, sensitivity analysis and parameter identification of a twin screw extruder
Grimard, Jonathan; Dewasme, Laurent; Thiry, Justine ULg et al

Conference (2016, June)

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See detailHot Melt Extrusion as a New Method to Form Inclusion Complexes with Cyclodextrins
Thiry, Justine ULg; Krier, Fabrice; Ratwatte, Shenelka et al

Conference (2016, April 06)

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See detailNanocarriers for the treatment of glioblastoma multiforme: Current state-of-the-art
Karim, Reatul ULg; Palazzo, Claudio ULg; Evrard, Brigitte ULg et al

in Journal of Controlled Release (2016)

Glioblastoma multiforme, a grade IV glioma, is the most frequently occurring and invasive primary tumor of the central nervous system, which causes about 4% of cancer-associated-deaths, making it one of ... [more ▼]

Glioblastoma multiforme, a grade IV glioma, is the most frequently occurring and invasive primary tumor of the central nervous system, which causes about 4% of cancer-associated-deaths, making it one of the most fatal cancers. With present treatments, using state-of-theart technologies, the median survival is about 14 months and 2 year survival rate is merely 3–5%. Hence, novel therapeutic approaches are urgently necessary. However, most drug molecules are not able to cross the blood–brain barrier, which is one of the major difficulties in glioblastoma treatment. This review describes the features of blood–brain barrier, and its anatomical changes with different stages of tumor growth. Moreover, various strategies to improve brain drug delivery i.e. tight junction opening, chemical modification of the drug, efflux transporter inhibition, convection-enhanced delivery, craniotomy-based drug delivery and drug delivery nanosystems are discussed. Nanocarriers are one of the highly potential drug transport systems that have gained huge research focus over the last few decades for site specific drug delivery, including drug delivery to the brain. Properly designed nanocolloids are capable to cross the blood–brain barrier and specifically deliver the drug in the brain tumor tissue. They can carry both hydrophilic and hydrophobic drugs, protect them from degradation, release the drug for sustained period, significantly improve the plasma circulation half-life and reduce toxic effects. Among various nanocarriers, liposomes, polymeric nanoparticles and lipid nanocapsules are the most widely studied, and are discussed in this review. For each type of nanocarrier, a general discussion describing their composition, characteristics, types and various uses is followed by their specific application to glioblastoma treatment. Moreover, some of the main challenges regarding toxicity and standardized evaluation techniques are narrated in brief. [less ▲]

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See detailSelection of a discriminant and biorelevant in vitro dissolution test for the development of fenofibrate self-emulsifying lipid-based formulations
Pestieau, Aude ULg; Krier, Fabrice; Brouwers, Adeline et al

in European Journal of Pharmaceutical Sciences (2016)

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See detailInvestigation of a suitable in vitro dissolution test for itraconazole-based solid dispersions
Thiry, Justine ULg; Broze, Guy ULg; Pestieau, Aude ULg et al

in European Journal of Pharmaceutical Sciences (2016)

Detailed reference viewed: 78 (33 ULg)
See detailPegylated Polyplexes Based On HDAC5 siRNA And Aliphatic Polycarbonate Polymers For An Anticancer Therapy
Frère, Antoine ULg; Baroni, Alexandra; Peulen, Olivier ULg et al

Poster (2015, November 23)

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See detailIn vitro and in vivo evaluation of an optimized fenofibrate lipid based solid dispersion produced by a PGSS process
Pestieau, Aude ULg; Lebrun, Sonia; Cahay, Bernard et al

Conference (2015, October 28)

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See detailIn vitro and in vivo evaluation of an optimized fenofibrate lipid based solid dispersion produced by a PGSS process
Pestieau, Aude ULg; Lebrun, Sonia; Cahay, Bernard et al

Poster (2015, October 28)

Detailed reference viewed: 29 (4 ULg)