References of "Du Jardin, Patrick"
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See detailCharacterization of volatile organic compounds emitted by Barley (Hordeum vulgare L.) roots and their attractiveness to wireworms
Gfeller, Aurélie; Laloux, Morgan; Barsics, Fanny ULg et al

in Journal of Chemical Ecology (2013), 39(8), 1129-1139

Root volatile organic compounds (VOCs), their chemistry and ecological functions have garnered less attention than aboveground emitted plant VOCs. We report here for the first time on the identification ... [more ▼]

Root volatile organic compounds (VOCs), their chemistry and ecological functions have garnered less attention than aboveground emitted plant VOCs. We report here for the first time on the identification of VOCs emitted by barley roots (Hordeum vulgare L.). Twenty nine VOCs were identified from isolated 21-d-old roots. The detection of root volatiles was dependent on the medium used for root cultivation. From 7-d-old roots cultivated on sterile Hoagland gelified medium, 24 VOCs were identified, on sterile vermiculite 33 VOCs, and on non-sterile vermiculite 34 VOCs. The major VOCs identified were fatty acid derived compounds, including hexanal, methyl hexanoate, (E)-hex-2-enal, 2-pentylfuran, pentan-1-ol, (Z)-2-(pentenyl)-furan, (Z)-pent-2-en-1-ol, hexan-1-ol, (Z)-hex-3-en-1-ol, (E)-hex-2-en-1-ol, oct-1-en-3-ol, 2-ethylhexan-1-ol (likely a contaminant), (E)-non-2-enal, octan-1-ol, (2E,6Z)-nona-2,6-dienal), methyl (E)-non-2-enoate, nonan-1-ol, (Z)-non-3-en-1-ol, (E)-non-2-en-1-ol, nona-3,6-dien-1-ol and nona-2,6-dien-1-ol. In an olfactometer assay, wireworms (larvae of Agriotes sordidus Illiger, Coleoptera: Elateridae) were attracted to chemical cues emanating from barley seedlings. We discuss the role of individual root volatiles or a blend of the root volatiles detected here and their interaction with CO2for wireworm attraction. [less ▲]

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See detailImprovement of Ylang-Ylang Essential Oil Characterization by GCxGC-TOFMS
Brokl, Michal ULg; Fauconnier, Marie-Laure ULg; Benini, Céline et al

in Molecules (2013), 18(2), 1783-1797

A single fraction of essential oil can often contain hundreds of compounds. Despite of the technical improvements and the enhanced selectivity currently offered by the state-of-the-art gas chromatography ... [more ▼]

A single fraction of essential oil can often contain hundreds of compounds. Despite of the technical improvements and the enhanced selectivity currently offered by the state-of-the-art gas chromatography (GC) and mass spectrometry (MS) instruments, the complexity of essential oils is frequently underestimated. Comprehensive two-dimensional GC coupled to time-of-flight MS (GC×GC-TOFMS) was used to improve the chemical characterization of ylang-ylang essential oil fractions recently reported in a previous one-dimensional (1D) GC study. Based on both, the enhanced chromatographic separation and the mass spectral deconvolution, 161 individual compounds were identified and labeled as potentially characteristic analytes found in both low and high boiling fractions issued from distillation of mature ylang-ylang flowers. Compared to the most recent full GC-MS characterization, this represents 75 new compounds, essentially consisting of terpenes, terpenoid esters, and alcohols. [less ▲]

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See detailAllelopathic potential of Tunisian barley against weeds
Bouhaouel, Imen ULg; Gfeller, Aurélie; Fauconnier, Marie-Laure ULg et al

Poster (2012, December)

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See detailPlant growth- promoting rhizobacteria emit volatiles compounds with biostimulation activity in dicot and monocot plant species.
du Jardin, Patrick ULg; Delaplace, Pierre ULg; Varin, Sébastien et al

in Perata, Pierdomenico; Brown, Patrick; Ponchet, Michel (Eds.) Abstracts Book for Oral and Poster Presentations of the 1st World Congress on the use of Biostimulants in Agriculture (2012, November)

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See detailConservation and management of a threatened traditional agroresource, ylang-ylang Cananga odorata [Lam.] Hook f. & Thomson forma genuina, in the Indian Ocean islands
benini, céline; Mahy, Grégory ULg; jacquemin, jean-marie et al

in Crop Science (2012), 52(6), 2606-2618

In order to handle future economic, social and environmental changes, the assessment, management and conservation of the local genetic resources of cash crop species is a fundamental requirement. We ... [more ▼]

In order to handle future economic, social and environmental changes, the assessment, management and conservation of the local genetic resources of cash crop species is a fundamental requirement. We investigated the pattern of genetic and morphological diversity of Cananga odorata (Lam.) Hook f. & Thomson forma genuina, an important essential oil tree for the perfume industry in the introduction area of the western Indian Ocean islands. We sought to identify key elements for developing a conservation and management strategy for ylang-ylang genetic resources. Genetic and morphological variations were assessed using Amplified Fragments Length Polymorphism and morphometrics traits and information about farmers' practices were collected. The existence of substantial overall genetic diversity (HT = 0.2599) and the grouping of plantations into different genetic groups suggest that there have been a series of introduction events in the area, with limited exchanges of genetic material within and between islands, which is not what is suggested in the historical records. The morphological study revealed high phenotypic variability despite very similar agronomical practices throughout the studied area. The morphological and genetic variability might have been created and maintained without any planned or conscious management, and this has largely determined the genetic structuring in the area (11.74% genetic variation among islands and 20.68% among plantations). With this species, where past introduction events and farmers’ practices have shaped the genetic variation, on-farm preservation and the maintenance of the current management practices is recommended. Ex situ conservation efforts should also be undertaken, if economically affordable. [less ▲]

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See detailComparative Chemical and Molecular Variability of Cananga odorata (Lam.) Hook.f. Thomson forma genuina (Ylang-Ylang) in the Western Indian Ocean Islands: Implication for Valorization
Benini, Céline ULg; Mahy, Grégory ULg; Bizoux, Jean-Philippe ULg et al

in Chemistry & Biodiversity (2012), 9(7), 13891402

Cananga odorata (Lam.) Hook.f. Thomson forma genuina (Annonaceae) is a tropical tree, grown for the production of ylang-ylang essential oil, which is extracted from its fresh and mature flowers. Despite ... [more ▼]

Cananga odorata (Lam.) Hook.f. Thomson forma genuina (Annonaceae) is a tropical tree, grown for the production of ylang-ylang essential oil, which is extracted from its fresh and mature flowers. Despite its economic and social importance, very little information is available on its variability and the possible factors causing it. Therefore, the relationship between the genetic structure, revealed by amplified fragment length polymorphism (AFLP), and the essential oil chemical composition, determined by GC/MS analysis, of ylang-ylang grown in semi-managed systems in three Indian Ocean islands (Grande Comore, Mayotte, and Madagascar) was investigated. Our results revealed a low genetic variation within plantations and contrasted situations between islands. Variations of the chemical composition could be observed within plantations and between islands. The genetic differentiation pattern did not match the observed pattern of chemical variability. Hence, the chemical variation could not be attributed to a genetic control. As Grande Comore, Madagascar, and Mayotte present different environmental and agronomic conditions, it can be concluded that the influence of these conditions on the ylang-ylang essential oil composition is consistent with the patterns observed. Finally, several strategies were proposed to valorize the chemical composition variations. [less ▲]

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See detailRhizobacterial volatile organic compounds modulate biomass production and root architecture in Arabidopsis thaliana (L.) Heynh.
Varin, Sébastien; Saunier de Cazenave-Mendaluk, Magdalena ULg; Ormeno-Lafuente, Elena et al

Poster (2012, February 10)

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See detailPossible consequences of the overlap between the CaMV 35S promoter regions in plant transformation vectors used and the viral gene VI in transgenic plants.
Podevin, Nancy; du Jardin, Patrick ULg

in GM crops & food (2012), 3(4), 296-300

Multiple variants of the Cauliflower mosaic virus 35S promoter (P35S) are used to drive the expression of transgenes in genetically modified plants, for both research purposes and commercial applications ... [more ▼]

Multiple variants of the Cauliflower mosaic virus 35S promoter (P35S) are used to drive the expression of transgenes in genetically modified plants, for both research purposes and commercial applications. The genetic organization of the densely packed genome of this virus results in sequence overlap between P35S and viral gene VI, encoding the multifunctional P6 protein. The present paper investigates whether introduction of P35S variants by genetic transformation is likely to result in the expression of functional domains of the P6 protein and in potential impacts in transgenic plants. A bioinformatic analysis was performed to assess the safety for human and animal health of putative translation products of gene VI overlapping P35S. No relevant similarity was identified between the putative peptides and known allergens and toxins, using different databases. From a literature study it became clear that long variants of the P35S do contain an open reading frame, when expressed, might result in unintended phenotypic changes. A flowchart is proposed to evaluate possible unintended effects in plant transformants, based on the DNA sequence actually introduced and on the plant phenotype, taking into account the known effects of ectopically expressed P6 domains in model plants. [less ▲]

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See detailScaphandre La science rencontre l'art: L'art
Haubruge, Eric ULg; Bay, Daniel ULg; Semal, Jean et al

in Haubruge, Eric; Bay, Daniel; Semal, Jean (Eds.) Scaphandre La science rencontre l'art (2012)

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See detailVariations in the essential oils from ylang-ylang (Cananga odorata [Lam.] Hook f. & Thomson forma genuina) in the Western Indian Ocean islands
Benini, Céline; Ringuet, Mélanie; Wathelet, Jean-Paul ULg et al

in Flavour and Fragrance Journal (2012), 27(5), 356-366

Ylang-ylang essential oil is an important rawmaterial for the fragrance industry. Despite its economic importance, to the best of our knowledge, no study has yet been undertaken to assess the chemical ... [more ▼]

Ylang-ylang essential oil is an important rawmaterial for the fragrance industry. Despite its economic importance, to the best of our knowledge, no study has yet been undertaken to assess the chemical polymorphism of the different production areas. This underestimated variability is an interesting source of raw material for perfumers. That is why the variation in the chemical composition of four fractions of the essential oils extracted from Cananga odorata, grown in four locations Grande Comore, Mayotte, Nossi Bé and Ambanja, was studied. A total of 119 compounds, representing 85.7–96.4%of the total essential oil composition, were identified using gas chromatography–mass spectrometry and quantified by gas chromatography with a flame ionization detector. Thirty-two compounds previously unreported in ylang-ylang essential oil were identified. The distinction between the Comoros and Madagascar groups wasmade on the basis of the chemical classes. It was possible to significantly distinguish the Grande Comore and Mayotte essential oil samples, as well as the Ambanja and Nossi Bé essential oil samples, on the basis of their main compounds. The aromatic compounds profile for the origin of each essential oil fraction was established. <br />Regression trees were built, allowing the provenance of the essential oils prepared at the laboratory level to be easily differentiated on the basis of a limited number of major compounds. [less ▲]

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See detailOrgan-dependent oxylipin signature in leaves and roots of salinized tomato plants (Solanum lycopersicum)
Ghanem, Michel Edmond; Ghars, Mohamed ali; Frettinger, Patrick et al

in Journal of Plant Physiology (2012), 169

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See detailDescribing and modelling root and shoot growth and development in Brachypodium distachyon (L.) Beauv
Delory, Benjamin ULg; Delaplace, Pierre ULg; Gfeller, Aurélie ULg et al

Poster (2011, October 19)

Due to its small size, its short developmental cycle and its close phylogenetic relationship with the Triticeae tribe, Brachypodium distachyon (L.) Beauv. has been proposed as a model species for ... [more ▼]

Due to its small size, its short developmental cycle and its close phylogenetic relationship with the Triticeae tribe, Brachypodium distachyon (L.) Beauv. has been proposed as a model species for temperate cereals. In this context, this work aims to describe and model root and shoot growth and development of B. distachyon (Bd21-1) grown under controlled environmental conditions [22°C, 65% RH, 20h light, 95 µmol.m-2.s-1 (PAR, LED lighting)]. For this purpose, vernalized caryopses were sown in a substrate consisting of vermiculite and compost (80/20, v/v). Growth and development of the above and belowground parts were monitored for 70 days. Dry and fresh masses of plant organs were measured every seven days from sowing. Biomasses of adventitious and seminal roots were measured separately. The number of spikelets on the main stem and on tillers was also counted on plants aged of 70 days. The modelling of root and shoot growth was achieved by calibrating sigmoidal growth models to the mean biomass values measured at each day of analysis. For each plant organ, the growth model selected was the one with the lowest residual variance. Finally, developmental stages identified for B. distachyon were compared with those defined for cereal crops by Zadoks et al. (1974). Maximum rates of fresh and dry shoot biomass production were 29,5 and 14,2 mg.day-1 respectively. Based on modelling, these values seem to be reached 49 and 72 days after sowing. Results also show that the fresh mass of adventitious roots at day 42 is significantly higher than that of seminal roots. Maximum rates of fresh and dry root biomass production were 6,9 and 0,8 mg.day-1 respectively, and were reached after 37 and 43 days. [less ▲]

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