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See detailThe A5 gene of alcelaphine herpesvirus 1 encodes a constitutively active G-protei n-coupled receptor that is non-essential for the induction of malignant catarrhal fever in rabbits
Boudry, Christel ULg; Markine-Goriaynoff, Nicolas ULg; Delforge, Cédric ULg et al

in Journal of General Virology (2007), 88(Pt 12), 3224-3233

Many gammaherpesviruses encode G-protein-coupled receptors (GPCRs). Several in vivo studies have revealed that gammaherpesvirus GPCRs are important for viral replication and for virus-induced pathogenesis ... [more ▼]

Many gammaherpesviruses encode G-protein-coupled receptors (GPCRs). Several in vivo studies have revealed that gammaherpesvirus GPCRs are important for viral replication and for virus-induced pathogenesis. The gammaherpesvirus alcelaphine herpesvirus 1 (AlHV-1) is carried asymptomatically by wildebeest, but causes malignant catarrhal fever (MCF) following cross-species transmission to a variety of susceptible species. The A5 ORF of the AlHV-1 genome encodes a putative GPCR. In the present study, we investigated whether A5 encodes a functional GPCR and addressed its role in viral replication and in the pathogenesis of MCF. In silico analysis supported the hypothesis that A5 could encode a functional GPCR as its expression product contained several hallmark features of GPCRs. Expression of A5 as tagged proteins in various cell lines revealed that A5 localizes in cell membranes, including the plasma membrane. Using [35S]GTPgammaS and reporter gene assays, we found that A5 is able to constitutively couple to alpha i-type G-proteins in transfected cells, and that this interaction is able to inhibit forskolin-triggered cAMP response element-binding protein (CREB) activation. Finally, using an AlHV-1 BAC clone, we produced a strain deleted for A5 and a revertant strain. Interestingly, the strain deleted for A5 replicated comparably to the wild-type parental strain and induced MCF in rabbits that was indistinguishable from that of the parental strain. The present study is the first to investigate the role of an individual gene of AlHV-1 in MCF pathogenesis. [less ▲]

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See detailA thromboxane receptor antagonist, reduces development of atherosclerosis in apoE-deficient mice.
Cherdon, Céline ULg; Rolin, S.; Hanson, S. et al

in Journal of Molecular and Cellular Cardiology (2007), 42

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See detailBM-573, a thromboxane receptor antagonist, reduces development of atherosclerosis in apoE–deficient mice
Cherdon, Céline ULg; Rolin, Stéphanie; Hanson, Julien ULg et al

in Journal of Molecular and Cellular Cardiology (2007), 42(suppl 1.), 33-34

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See detailNatural antibody--complement dependent neutralization of bovine herpesvirus 4 by human serum
Machiels, Bénédicte ULg; Gillet, Laurent ULg; Brito, Sieberth Do Nascimento et al

in Microbes & Infection (2007), 9(14-15), 1530-1537

In contrast to most gammaherpesviruses, Bovine herpesvirus 4 (BoHV-4) has a broad range of host species both in vitro and in vivo. Several in vitro studies demonstrated that some human cell lines are ... [more ▼]

In contrast to most gammaherpesviruses, Bovine herpesvirus 4 (BoHV-4) has a broad range of host species both in vitro and in vivo. Several in vitro studies demonstrated that some human cell lines are sensitive or even permissive to BoHV-4. These observations led to the hypothesis that cross-species transmission of BoHV-4 could lead to human infections. In the present study, we investigate the sensitivity of BoHV-4 to neutralization by naïve human sera in order to determine if humans exhibit innate anti-viral activities against this virus. Our results demonstrate that human sera from naïve individuals, in contrast to the sera of naïve subjects from various animal species, neutralize BoHV-4 efficiently. A series of complementary experiments were performed to unravel the mechanism(s) of this neutralization. The data obtained in this study demonstrates that human serum neutralizes BoHV-4 in a complement dependent manner activated by natural antibodies raised against the Galalpha1-3Galbeta1-4GlcNAc-R epitope expressed by bovine cells [less ▲]

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See detailEstablishment and characterisation of two novel human KSHV- and EBV-negative Burkitt cell lines, GAL-01 and GAL-02, from a primary lymphomatous effusion
Thielen, Caroline ULg; Herens, Christian ULg; Fassotte, Marie-France ULg et al

in European Journal of Haematology (2006), 77(4), 318-326

Objectives: Burkitt's lymphoma (BL) is a highly aggressive mature B-cell neoplasm comprising endemic, sporadic and immunodeficiency-associated variants. Human cell lines constitute a very useful tool to ... [more ▼]

Objectives: Burkitt's lymphoma (BL) is a highly aggressive mature B-cell neoplasm comprising endemic, sporadic and immunodeficiency-associated variants. Human cell lines constitute a very useful tool to investigate the biology of lymphoid neoplasia. In this study, we succeeded in establishing two human cell lines, GAL-01 and GAL-02, from a HIV-negative patient with Epstein-Barr virus (EBV) -negative sporadic BL presenting as an effusion. GAL-01 and GAL-02 were established at diagnosis and after one course of polychemotherapy, respectively. The in vivo effusion occurred in a very peculiar clinical setting; the patient having a previous history of intestinal diffuse large B-cell lymphoma. Methods: The morphologic, immunophenotypic and molecular genetic features of GAL cell lines are reported and compared with those of the parental tumour. The findings clearly demonstrated that the Burkitt effusion did not represent disease progression of the intestinal tumour, but represented a second primary haematological malignancy. The in vivo tumorigenic properties of the cells were tested by subcutaneous injection to NOD/SCID mice. Results: Both cell lines were composed of medium-sized lymphoid cells with clumped chromatin, multiple medium-sized nucleoli and moderate amounts of vacuolated cytoplasm. GAL cells display the phenotype and genotype of a B-cell lineage (positive for CD20, CD79a and clonal rearrangement of Ig heavy chain), carry the c-MYC rearrangement by t(8;22)(q24;q11) translocation and are characterised by the expression of the germinal centre-associated antigens CD10, BCL6, CD38 and absent to low BCL2 expression. EBV and HHV8 were not identified within parental tumour or in cultured cells. Subcutaneous injection of both cell lines to NOD/SCID mice induced tumour formation. Conclusions: GAL-01 and GAL-02, two novel EBV-negative human BL cell lines represent a potentially useful experimental model to study the biology of BL possibly including the resistance to chemotherapy. [less ▲]

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See detailThe use of an apoE–deficient (apoE-/-) mice model to characterize the therapeutic benefits of original thromboxane modulators
Cherdon, Céline ULg; Rolin, stephanie; Hanson, Julien ULg et al

Poster (2006, May 17)

The aim of our study was to use an apoE–deficient mouse model to test drugs acting as thromboxane A2 antagonist. Presented here is the “en face” method 6 which allows the evaluation of atherosclerosis ... [more ▼]

The aim of our study was to use an apoE–deficient mouse model to test drugs acting as thromboxane A2 antagonist. Presented here is the “en face” method 6 which allows the evaluation of atherosclerosis lesions development in wild type and APO E-/- mice. This method involves pinning out the aorta and quantifying lesion area as a percentage of total surface area. The use of this mice model offers a unique opportunity to characterize the therapeutic benefits of pharmacologicals agents designed in our laboratory of which antioxidants and thromboxane modulators. The pharmacological characterization of BM-573 as potential antiatherosclerotic agent will also be discussed [less ▲]

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See detailTowards a better use of the rabbit as an experimental model: review and perspectives
Dewree, R.; Drion, Pierre ULg

in Annales de Médecine Vétérinaire (2006), 150(3), 153-162

The rabbit is an essential model in scientific research, particularly in the study of cardiovascular diseases such as hypertension and atherosclerosis and for the investigation of the osteoarticular ... [more ▼]

The rabbit is an essential model in scientific research, particularly in the study of cardiovascular diseases such as hypertension and atherosclerosis and for the investigation of the osteoarticular system. Due to the fact that various factors (environmental, microbial, stress, pain.) can influence data obtained from experiments, special care must be given to housing conditions. Sensory enrichment (olfactive, auditive, visual or tactile stimuli) and physical enrichment (nutritional and social stimuli) allow rabbits to express specific behaviours and reduce stereotypies. This synthesis reminds of the importance of these factors and gives to the researcher some ways to control them. [less ▲]

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See detailFrom the design to the clinical application of thromboxane modulators
Dogné, Jean-Michel ULg; Hanson, Julien ULg; de Leval, Xavier et al

in Current Pharmaceutical Design (2006), 12(8), 903-923

Arachidonic acid (AA) metabolites are key mediators involved in the pathogenesis of numerous cardiovascular, pulmonary, inflammatory, and thromboembolic diseases. One of these bioactive metabolites of ... [more ▼]

Arachidonic acid (AA) metabolites are key mediators involved in the pathogenesis of numerous cardiovascular, pulmonary, inflammatory, and thromboembolic diseases. One of these bioactive metabolites of particular importance is thromboxane A(2) (TXA(2)). It is produced by the action of thromboxane synthase on the prostaglandin endoperoxide H-2 (PGH(2)) which results from the enzymatic transformation of AA by the cyclooxygenases. It is a potent inducer of platelet aggregation, vasoconstriction and bronchoconstriction, and has been involved in a series of major pathophysiological conditions. Therefore, TXA(2) receptor antagonists, thromboxane synthase inhibitors and drugs combining both properties have been developed by different laboratories since the early 1980s. Several Compounds have been launched on the market and others are tinder clinical evaluation. In the first part of this review. we will describe the physiological properties of TXA(2), thromboxane synthase and thromboxane receptors. The second part is dedicated to a description of each class of thromboxane modulators with the advantages and disadvantages they offer. In the third part. we aim to describe recent studies performed with the most interesting thromboxane modulators in major pathologies: myocardial infarction and thrombosis, atherosclerosis, diabetes, pulmonary embolism, septic shock.. preeclampsia, and asthma. Each pathology will be systematically reviewed. Finally, in the last part we will highlight the latest perspectives in drug design of thromboxane modulators and in their future therapeutic applications such as cancer, metastasis and angiogenesis. [less ▲]

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See detailPhysiologie de la Reproduction animale TOME 3 (de 3)
Drion, Pierre ULg; Beckers, Jean-François ULg; Derivaux, Jules et al

Learning material (2005)

Ce document reprend le 3è TOME de "Physiologie de la Reproduction" de "Drion, Beckers, Derivaux, Hanzen, Ectors". Daprès le même intitulé ("Derivaux, Ectors, Reproduction chez les animaux domestiques ... [more ▼]

Ce document reprend le 3è TOME de "Physiologie de la Reproduction" de "Drion, Beckers, Derivaux, Hanzen, Ectors". Daprès le même intitulé ("Derivaux, Ectors, Reproduction chez les animaux domestiques", Cabay, Louvain la neuve, 1986), revisité et mis à jour, avec l'aide des auteurs, annuellement de 2000 à 2005. Il constitue la base de l'enseignement du cours de 3è Bac en Médecine Vétérinaire à l'Université de Liège. [less ▲]

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See detailPhysiologie de la Reproduction animale TOME 2 (de 3)
Drion, Pierre ULg; Beckers, Jean-François ULg; Derivaux, Jules et al

Learning material (2005)

Ce document reprend le 2è TOME de "Physiologie de la Reproduction" de "Drion, Beckers, Derivaux, Hanzen, Ectors". Daprès le même intitulé ("Derivaux, Ectors, Reproduction chez les animaux domestiques ... [more ▼]

Ce document reprend le 2è TOME de "Physiologie de la Reproduction" de "Drion, Beckers, Derivaux, Hanzen, Ectors". Daprès le même intitulé ("Derivaux, Ectors, Reproduction chez les animaux domestiques", Cabay, Louvain la neuve, 1986), revisité et mis à jour, avec l'aide des auteurs, annuellement de 2000 à 2005. Il constitue la base de l'enseignement du cours de 3è Bac en Médecine Vétérinaire à l'Université de Liège. [less ▲]

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See detailPhysiologie de la Reproduction animale TOME 1 (de 3)
Drion, Pierre ULg; Beckers, Jean-François ULg; Derivaux, Jules et al

Learning material (2005)

Ce document reprend le 1er TOME de "Physiologie de la Reproduction" de "Drion, Beckers, Derivaux, Hanzen, Ectors". Daprès le même intitulé ("Derivaux, Ectors, Reproduction chez les animaux domestiques ... [more ▼]

Ce document reprend le 1er TOME de "Physiologie de la Reproduction" de "Drion, Beckers, Derivaux, Hanzen, Ectors". Daprès le même intitulé ("Derivaux, Ectors, Reproduction chez les animaux domestiques", Cabay, Louvain la neuve, 1986), revisité et mis à jour, avec l'aide des auteurs, annuellement de 2000 à 2005. Il constitue la base de l'enseignement du cours de 3è Bac en Médecine Vétérinaire à l'Université de Liège. [less ▲]

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See detailCharacterization of an original model of myocardial infarction provoked by coronary artery thrombosis induced by ferric chloride in pig
Dogné, Jean-Michel ULg; Rolin, Stéphanie; Petein, Michel et al

in Thrombosis Research (2005), 116(5), 431-442

Background: Great advances have been made in the prevention of thrombotic disorders by developments of new pharmacological and surgical treatments. Animal models of arterial thrombosis have largely ... [more ▼]

Background: Great advances have been made in the prevention of thrombotic disorders by developments of new pharmacological and surgical treatments. Animal models of arterial thrombosis have largely contributed to the discovery and to the validation of original treatments. The purpose of the present work was to develop and validate an original model of acute myocardial infarction provoked in pig by thrombosis of the left anterior descending (LAD) coronary artery induced by topical application of ferric chloride solution. Methods and results: Myocardial infarction, resulting from an occlusive and adherent mixed thrombus formed in the LAD coronary artery, was examined at macroscopic level using dual staining technique (Evans blue dye; triphenyltetrazolium chloride) and at microscopic level using conventional histological analyses and immunohistochemical detection of desmin. Biochemical markers (troponin T and ATP), platelet reactivity and standard hemodynamic parameters (such as stroke volume, ejection fraction, stroke work and cardiac output) have also been evaluated. From these analyses, it was demonstrated that each pig developed a transmural area of irreversible damage mainly located in the anteroseptal region of the left ventricle. The more progressive development of coronary artery occlusion, as compared to an abrupt Ligation, was accompanied by a correspondingly progressive impairment in hemodynamics. Conclusion: We conclude that this original porcine model of myocardial infarction is quite close to clinical pathophysiological conditions, such as thrombus formation occurring after atherosclerotic plaque rupture. This certainly constitutes a further argument in favour of this model to assess pharmaceutical or mechanical support of an acutely ischemic heart. (c) 2005 Elsevier Ltd. All rights reserved. [less ▲]

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See detailUtilisation raisonnable des gonadotrophines exogènes dans le contrôle de la reproduction
Drion, Pierre ULg

in Folia Veterinaria (2005)

De nombreuses substances pharmacologiques (tableau 1) incluant des stéroïdes, des peptides et des glycoprotéines potentiellement immunogènes sont fréquemment utilisées dans les programmes thérapeutiques ... [more ▼]

De nombreuses substances pharmacologiques (tableau 1) incluant des stéroïdes, des peptides et des glycoprotéines potentiellement immunogènes sont fréquemment utilisées dans les programmes thérapeutiques ou de gestion de la reproduction chez les animaux domestiques et dans les programmes de procréation assistée chez l'homme. De nombreuses investigations non invasives ont été menées dans différentes espèces en vue de récolter des informations objectives sur les effets secondaires possibles d'administrations répétées de gonadotrophines exogènes, tels la présence d'anticorps circulants et la diminution de la réponse biologique aux traitements. De manière synthétique, il apparaît que l'exposition répétée d'un animal à une molécule gonadotrope exogène, entraîne chez celui-ci la formation d'anticorps spécifiques dirigés contre cette molécule. Cependant, les taux de liaison des anticorps plasmatiques aux gonadotrophines injectées ne suffisent pas à expliquer l'intégralité d'une modification des performances de reproduction. La littérature portant sur les programmes d'immunisation contre les stéroïdes ou l'inhibine -utilisés pour améliorer les performances de reproduction- constitue une bonne illustration du contrôle multifactoriel de telles régulations et de la difficulté à obtenir les effets souhaités. [less ▲]

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See detailLes protéines de choc thermique (heat shock proteins). II Les protéines de choc thermique (heat shock proteins). II. Hsp70, biomarqueur, bioprotecteur.
Wirth, Delphine; Gustin, Pascal ULg; Drion, Pierre ULg et al

in Annales de Médecine Vétérinaire (2003), 147(2), 127-144

The exposure of any organism to high temperature induces rapid and transient cellular overexpression of specific proteins, the heat shock proteins (Hsps). This response, called heat shock response, was ... [more ▼]

The exposure of any organism to high temperature induces rapid and transient cellular overexpression of specific proteins, the heat shock proteins (Hsps). This response, called heat shock response, was initially discovered in Drosophila. The genes hsps were among the first eukaryotic genes to be cloned and whose regulation, which involves activation of heat shock factor (HSF), was elucidated. More recently, study of Hsp functions, particularly Hsp70, started. Their protective role, related to their molecular chaperon function, was inferred from experiments showing that Hsp70 expression, induced by a first stress, results in a cellular tolerance to second stress. Better understanding of the heat shock response had led to the development of two search fields on Hsp70 expression : (1) its use as biomarker of cellular stress and (2) the exploitation of its cytoprotective functions against various insults. This review includes historic of the research on the heat shock response, description of regulation mechanism of Hsp70 expression, and the interesting perspectives allocated to Hsp70 as biomarker and therapeutic tool. ----------- L’exposition de tout organisme à des températures élevées induit l'expression cellulaire rapide et transitoire de protéines spécifiques, les protéines de choc thermique (Hsps pour " heat shock proteins "). Cette réponse des cellules au choc thermique ou " heat shock response " a été initialement découverte chez la drosophile. Les gènes hsps furent parmi les premiers gènes eucaryotes à être clonés et utilisés comme paradigme dans l'étude des mécanismes de régulation transcriptionnelle faisant intervenir l'activation d'un ou plusieurs " heat shock factors" (HSFs). C'est plus récemment que l'étude des fonctions des Hsps, particulièrement Hsp70, a débuté. Le rôle protecteur de celle-ci, lié à sa fonction de " chaperon " protéique, a été déduit d'expériences montrant que l'induction de l'expression de Hsp70 lors d'un stress était associée au développement d'une tolérance cellulaire vis-à-vis d'un stress ultérieur. En plus d'un intérêt fondamental pour la compréhension de la "réponse au choc thermique", deux axes de recherche appliquée se sont développés visant à investiguer la possibilité d'utiliser l'expression de Hsp70 comme biomarqueur de souffrance cellulaire d'une part et, d'autre part, d'exploiter ses fonctions comme moyen de protection des cellules contre divers types d'agressions. Cette revue décrit l'historique des découvertes sur la " réponse au choc thermique ", le mécanisme régulant l'expression de Hsp70 ainsi que les perspectives intéressantes s'ouvrant à l'utilisation de l'expression de Hsp70 en tant que biomarqueur et outil thérapeutique. [less ▲]

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See detailThe use of blood pepsinogen as a biomarker of the integrity of the porcine gastric mucosa. 2. Measurements of blood pepsinogen and its usefull in the detection of gastric diseases
Banga-Mboko, Henri; Godeau, Jean-Marie ULg; Drion, Pierre ULg et al

in Annales de Médecine Vétérinaire (2003), 147(2, APR-MAY), 95-104

Pepsinogen is one component of the gastric juice which participes in the digestion. This macromolecule enters the blood circulation in a small measurable quantities in healthy subjects. Therefore, blood ... [more ▼]

Pepsinogen is one component of the gastric juice which participes in the digestion. This macromolecule enters the blood circulation in a small measurable quantities in healthy subjects. Therefore, blood pepsinogen is claimed to be an indicator of the integrity of the gastric mucosa. This paper was written to review the use of porcine in the diagnostic of stomach ulcers and Hyostrongylus rubidus infection. The methods of measurement of blood pepsinogen and the diagnostic values are discussed. [less ▲]

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See detailPhysiology of reproduction and endocrinology in cervids. A review
Drion, Pierre ULg; Hanzen, Christian ULg; Wirth, Delphine - in memoriam et al

in Annales de Médecine Vétérinaire (2003), 147(5), 291-313

Knowledge about cervids are rapidly growing. This work aims to present the more recent scientific information on reproductive physiology and endocrinology of the cervids: anatomy of the genital tract ... [more ▼]

Knowledge about cervids are rapidly growing. This work aims to present the more recent scientific information on reproductive physiology and endocrinology of the cervids: anatomy of the genital tract, length of breeding season, parameters and endocrinology of the reproductive cycle, endocrinology of pregnancy. It also aims to highlight differences between cervids and domestic ruminants such as embryonic diapause observed in Capreolus capreolus. [less ▲]

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