References of "Dreesen, Laurent"
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See detailSuccessive Droplets Encapsulation Revealed by Optofluidics
Lismont, Marjorie ULiege; Robert, Damien; Vandewalle, Nicolas ULiege et al

Poster (2013, November)

Digital microfluidics: an easy way to encapsulate successively water droplets by oil. Digital microfluidics is implemented to coat successively water droplets with a thin layer of silicon oil. Small water ... [more ▼]

Digital microfluidics: an easy way to encapsulate successively water droplets by oil. Digital microfluidics is implemented to coat successively water droplets with a thin layer of silicon oil. Small water droplets are settled at three fibers nodes while a larger oil drop slides down the vertical fiber. Optofluidics: an interesting way to reveal the droplet sliding motion and the encapsulation. To distinguish the different fluids, quantum dots (QDs) and rhodamine labelled polystyrene beads are diluted in oil and water, respectively.These two fluorescent probes are lighted by a 532 nm radiation.The fluorescence signal is recorded by a high speed camera, perpendicularly to the laser and to the fibers network. This set-up reveals that the oil drop passes through the water droplets without removing them from the nodes, leaving a thin oil layer around the water droplets. The coating, clearly seen on top pictures of Fig 2, prevents water evaporation. The key parameters for a good encapsulation are the fiber diameter and the oil viscosity that determine droplets size and velocity, respectively. [less ▲]

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See detailEncapsulation of water droplets on fibers
Weyer, Floriane ULiege; Dreesen, Laurent ULiege; Vandewalle, Nicolas ULiege

Poster (2013, November)

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See detailComparative study of Ag and Au nanoparticles biosensors based on surface plasmon resonance phenomenon
Lismont, Marjorie ULiege; Dreesen, Laurent ULiege

in Materials Science & Engineering : C (2012)

The specific sensitivity of surface plasmon resonance to changes in the local environment of nanoparticles allows their use as platforms to probe chemical and biochemical binding events on their surfaces ... [more ▼]

The specific sensitivity of surface plasmon resonance to changes in the local environment of nanoparticles allows their use as platforms to probe chemical and biochemical binding events on their surfaces without any labeling [1- 4]. In this paper, we perform a comparative study of gold and silver nanoparticle based biosensors, prepared within the same conditions, in order to determine which metal seems the best for biological sensing. The prototypical biocytin-avidin interaction is used to study gradual changes over time and with avidin concentration in the absorption spectra bands of biocytinylated 10 nm silver and gold nanospheres. First, the Ag nanoparticles plasmon resonance absorbance signal is about 10 times larger than the Au one. Secondly, for an equivalent concentration of avidin, the optical properties modifications are more pronounced for silver nanoparticles than for gold ones of the same geometry. These observations attest the superiority of Ag on Au nanoparticles when optical considerations are only taken into account. Finally, with both biosensors, the specificity of the interaction, checked by replacing avidin with bovine serum albumin, is relatively poor and needs to be improved. [less ▲]

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See detailCOMPARATIVE STUDY OF SPR BIOSENSORS BASED GOLD AND SILVER COLLOIDAL NANOPARTICLES
Lismont, Marjorie ULiege; Dreesen, Laurent ULiege

Poster (2011, June)

Noble metal nanoparticles (NPs) can be used as a robust tool for optical bio-sensing. These NPs are known for their strong interactions with light through their surface plasmon resonance (SPR), which ... [more ▼]

Noble metal nanoparticles (NPs) can be used as a robust tool for optical bio-sensing. These NPs are known for their strong interactions with light through their surface plasmon resonance (SPR), which corresponds to the collective oscillations of the conduction electrons on the particles [1]. Among metals, silver and gold NPs are of particular interest not only because they are air-stable but also because their SPR absorption bands are in the visible and near ultra-violet spectral regions, that appear as the most appropriate for technological applications [2]. The first advantage of such optical SPR biosensors is their ability to measure complex formation in real time. Indeed, the SPR absorption spectrum band of the NPs is sensitive to the shape, size, inter-particle distance and composition of the NP as well as the dielectric properties of the surrounding medium [2]. Because of the sensitivity of SPR to the local dielectric environment, plasmonic NPs can act as transducers that convert small changes in the local refractive index and the inter-particle distance into spectral shifts and broadening in the absorption spectra bands [3]. Biotin is a water-soluble B complex vitamin necessary for the production of fatty acids and the metabolism of fats and amino acids. The avidin is a tetrameric protein which can react with biotin to form the strongly bonded biotin-avidin complex.The prototypical biotin-avidin interaction forms the basis of a simple sol-based diagnostic technique for biological analytes. We focused on this well-known couple of bio-molecules to compare optical properties of silver and gold colloidal NPs. Gradual changes with time in the absorption spectra bands of biotinylated 10 nm silver and gold NPs were studied as a function of added avidin. After avidin addition, an increased red-shift of the SPR wavelength and a broadening of the absorption band with time are observed. These changes in the optical properties of colloidal NPs are due to the biomolecular recognition process between biotin and avidin which leads to aggregation of these NPs arising from cross-linking by the tetrameric protein. Moreover, the recognition process induces a variation of the local refractive index around these NPs and thus induces a red-shift of SPR also. The maximum SPR red-shift was reached after 45 minutes and was equal to 25 nm and 15 nm for silver NPs and gold NPs respectively. We concluded that the dielectric sensitivity of gold NPs is smaller than the silver NPs one for a same geometry and for an equivalent concentration of avidin. Therefore, the silver sol is more adapted to detection of avidin than the gold sol. The detection limit, described as the lowest concentration for clear identification of wavelength shift [4] due to biomolecular recognition is determined to be 4 nM for both silver and gold NPs. In this case, the corresponding wavelength shift is about 3 nm. The specificity of the interaction between biocytin and avidin was checked by replacing avidin by BSA. When BSA was added, we observed a SPR shift which was smaller than the detection limit of 3 nm. Future works will be devoted to transpose this kind of biomolecular recognition experiments on gold nanorods in order to improve the dynamic phototherapy efficiency of cancers. [less ▲]

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See detailComparative study of gold and silver based nanobiosensors
Lismont, Marjorie ULiege; Dreesen, Laurent ULiege

Poster (2011, May 25)

Due to their particular optical properties, resulting from the surface plasmon resonance (SPR) phenomenon, silver and gold nanoparticles (NPs) can be used as robust tools for optical biosensing [1 ... [more ▼]

Due to their particular optical properties, resulting from the surface plasmon resonance (SPR) phenomenon, silver and gold nanoparticles (NPs) can be used as robust tools for optical biosensing [1]. Optical SPR biosensors are able to measure complex formation in real time. Indeed, the SPR absorption spectrum band of the NPs is sensitive to the shape, size, inter-particle distance and composition of the NP as well as the dielectric properties of the surrounding medium [2]. Due to the sensitivity of SPR to the local dielectric environment, plasmonic NPs can act as transducers that convert small changes in the local refractive index and the inter-particle distance into spectral shifts and broadening in the absorption spectra bands [3]. In our study, the prototypical biocytin-avidin interaction was used to study gradual changes with time in the absorption spectra bands of biotinylated 10 nm silver and gold NPs as a function of added avidin. After avidin addition, a SPR red-shift and a broadening of the SPR bands were observed with both NPs. These optical changes evolved with time and reached their final values after around 45 min for each system. The maximum SPR red-shifts were equal to 25 nm and 15 nm for silver NPs and gold NPs, respectively. The detection limit, described as the lowest concentration for clear identification of wavelength shift due to biomolecular recognition, is determined to be 4 nM for both silver and gold NPs. The specificity of the biocytin-avidin biosensors was checked by replacing avidin by BSA. When BSA was added, we observed a SPR band shift which was smaller than the detection limit of 3 nm, attesting the biosensor selectivity. Our work demonstrates the superiority of Ag over Au NPs for the elaboration of biosensors based on SPR. [less ▲]

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See detailGold and silver nanomaterials based biosensors : a comparative study
Dreesen, Laurent ULiege; Lismont, Marjorie ULiege

Poster (2011, April)

Noble metal nanoparticles (NPs) are intensively studied due to their particular optical properties, mainly high optical absorption and diffusion yields, leading to interesting applications in biochemical ... [more ▼]

Noble metal nanoparticles (NPs) are intensively studied due to their particular optical properties, mainly high optical absorption and diffusion yields, leading to interesting applications in biochemical sensing, molecular tracking and imaging, drug delivery and photothermal therapies [1]. These unique optical properties arise from a physical process named surface plasmon resonance (SPR) which is a resonant coupling of incident light to the collective motion of electrons along the nanoparticles surface [2]. Optical SPR biosensors are able to measure complex formation in real time. Indeed, the SPR absorption spectrum band of the NPs is sensitive to the shape, size, inter-particle distance and composition of the NP as well as the dielectric properties of the surrounding medium [2]. Due to the sensitivity of SPR to the local dielectric environment, plasmonic NPs can thus act as transducers that convert small changes in the local refractive index or in the inter-particle distance into spectral shifts and broadenings of the absorption spectral bands [3]. Among metals, silver and gold NPs have received considerable interest for many reasons. For instance, they are stable in ambient atmosphere and exhibit good biocompatibility even if particular surface treatments are sometimes required. The Ag and Au NPs are also relatively easy to fabricate with different sizes and shapes allowing the tuning of the SPR optical absorption band from the near ultraviolet (400 nm) to the near infrared (1000 nm) wavelengths. In this study, our aim is to characterize two biosensors based on silver and gold spherical NPs in order to detect which one seems the best. Both NPs have a diameter close to 15 nm. We use the well-known biocytin-avidin complex as a model system because the bonding of avidin with biocytin is extremely strong with a dissociation constant three order of magnitude higher than the typical constants of antigen-antibody interactions. More precisely, we compare the intensities, the band shapes and the spectral locations of the SPR adsorption bands before and after the biomolecular recognition of avidin by biocytin molecules adsorbed on the Ag and Au NPs. The kinetic of the interaction is also discussed. Before surface treatment with biocytin, the NPs SPR bands are located around 390 and 520 nm for Ag and Au NPs, respectively. The SPR band intensity is higher for silver than for gold. Biocytin adsorption does not significantly modify the SPR spectral features. NPs do not therefore form aggregates and the local refraction index has not significantly changed. After avidin addition, a SPR red-shift and a broadening of the SPR bands are observed with both NPs as shown on Figure 1. These parameters evolved with time and reach their final values after around 45 min for each system. The aforementioned spectral changes arise from the biomolecular recognition process between biotin and avidin which leads to the NPs aggregation. The recognition process also induces a variation of the local refractive index around these NPs which contributes to the red-shift. The maximum SPR shifts are equal to 25 nm and 12 nm for silver NPs and gold NPs, respectively. Our results highlight the smaller dielectric sensitivity of gold NPs compared to the silver NPs one for a same particles’ size and for an equivalent concentration of avidin. The detection limit, described as the lowest concentration for clear identification of wavelength shift due to biomolecular recognition, is equal to 4 nM for both silver and gold NPs. With this protein concentration, 3 nm is the typical wavelength shift. The specificity of the biocytin - avidin biosensors is verified by replacing avidin by Bovine Serum Albumina (BSA). When BSA is added, we observe a SPR band shift which is smaller than the detection limit of 3 nm attesting the biosensor selectivity. Our work demonstrates the superiority of Ag over Au NPs for the elaboration of biosensors based on SPR. However, it is well-known that Ag NPs are less biocompatible than gold. This problem can be circumvented by an appropriate coating of the NPs surface prior ligand adsorption. [less ▲]

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See detailSelf-Assembled Film Organization in Fast Microcontact Printing Investigated by Sum Frequency Generation Spectroscopy
Lis, Dan; Peremans, André; Sartenaer, Yannick et al

in Journal of Physical Chemistry C: Nanomaterials, Interfaces, and Hard Matter (2009), 113

The ability of microcontact printing to build highly ordered alkanethiol self-assembled monolayers (SAMs) on Pt substrates within a short time is studied by sum frequency generation (SFG) spectroscopy and ... [more ▼]

The ability of microcontact printing to build highly ordered alkanethiol self-assembled monolayers (SAMs) on Pt substrates within a short time is studied by sum frequency generation (SFG) spectroscopy and contact angle measurements. The deposition of ordered hexadecanethiol and dodecanethiol monolayers onto platinum substrates is achieved in less than 1 s. The film order and the alkane chain orientation are deduced from the SFG fingerprint acquired under different sets of laser polarization. Comparisons between the SAMs prepared by printing or by immersion demonstrate that both methods lead to the same high quality organization. Patterning effects within printed films are also investigated with respect to the layer conformation. Finally, wetting properties of printed layers are correlated with the printing duration, corroborating the spectroscopic results. [less ▲]

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See detailDielectric Multilayer Films Fabricated by Magnetron Sputtering: How Far Can the Iridescence Be Tuned?
Deparis, Olivier; Rassart, Marie; Vandenbem, Cedric et al

in Plasma Processes and Polymers (2009), 6

Periodic dielectric multilayer structures can generate interferential colours from optically transparent basic materials. Their iridescence property, i.e. the change of colour with the illumination or ... [more ▼]

Periodic dielectric multilayer structures can generate interferential colours from optically transparent basic materials. Their iridescence property, i.e. the change of colour with the illumination or viewing angle, is exploited in the industry to produce structurally coloured coatings and paints. Magnetron sputtering is an efficient technique for producing multilayer films owing to its ability to deposit films on large surfaces with excellent uniformity and reproducibility in both the film thickness and composition. Based on a theoretical model of the optical response, we investigated the iridescence tuning range of the technologically important SiO2/TiO2 material system. Radically different iridescent behaviours were predicted theoretically and demonstrated experimentally by selecting appropriate combinations of period and layer thickness ratio. [less ▲]

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See detailClose-packed array of gold nanoparticles and Sum Frequency Generation spectroscopy in total internal reflection: A platform for studying biomolecules and biosensors
Tourillon, Gérard; Dreesen, Laurent ULiege; Volcke, Cédric et al

in Journal of Materials Science (2009), 44(24), 6805-6810

An approach is introduced for studying the adsorption and recognition mechanisms of biomolecules, without using any markers. We show for the first time, that the Sum Frequency Generation spectroscopy ... [more ▼]

An approach is introduced for studying the adsorption and recognition mechanisms of biomolecules, without using any markers. We show for the first time, that the Sum Frequency Generation spectroscopy performed in the Total Internal Reflection (TIR-SFG) geometry, combined with a regular close-packed array of gold nanoparticles allows to probe with a high sensitivity the changes in conformation and orientation induced by the recognition process of avidin by biocytin. This approach represents a new platform with potential use in biosensors, diagnostics and bioactive layers. [less ▲]

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See detailDC Magnetron Sputtering Deposition of Titanium Oxide Nanoparticles: Influence of Temperature, Pressure and Deposition Time on the Deposited Layer Morphology, the Wetting and Optical Surface Properties
Dreesen, Laurent ULiege; Cecchet, Francesca; Lucas, Stéphane

in Plasma Processes and Polymers (2009), 6

Titanium dioxide nanoparticles were prepared on glass substrates by reactive DC magnetron sputtering. As highlighted by the atomic force microscopy characterization, we were able to control the ... [more ▼]

Titanium dioxide nanoparticles were prepared on glass substrates by reactive DC magnetron sputtering. As highlighted by the atomic force microscopy characterization, we were able to control the nanoparticles' surface coverage and diameter by varying the deposition time and the total pressure, respectively. The titanium dioxide energy band gap, determined by using ultraviolet-visible, spectroscopy, depends on the total pressure but is quite independent of the deposition temperature. On the contrary, it is blue shifted when the pressure increases. Finally, the contact angles slightly decrease after ultraviolet illumination irrespective of the different deposition parameters, indicating an improvement of the hydrophilic properties of the adsorbed layer. After 21 h in dark, the contact angles are nearly identical to the ones before exposure to UV light: the samples do not keep their hydrophilic behaviour. [less ▲]

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See detailSynthesis of titanium dioxide nanoparticles by reactive DC magnetron sputtering
Dreesen, Laurent ULiege; Colomer, Jean-François; Limage, Hervé et al

in Thin Solid Films (2009), 518

Nanometer-sized titanium dioxide (TiO2) particles were prepared on carbon substrates by reactive direct-current magnetron sputtering. By performing measurements with high resolution electron microscopes ... [more ▼]

Nanometer-sized titanium dioxide (TiO2) particles were prepared on carbon substrates by reactive direct-current magnetron sputtering. By performing measurements with high resolution electron microscopes, the mean nanoparticle diameter and the coverage fraction of the substrate by the nanoparticles (NPs) were measured at 19 nm and 30%, respectively. Moreover, electron diffraction analysis showed that the TiO2 NPs' crystalline structure on the carbon substrate was a mixture of anatase and rutile. Finally,we provided information on the TiO2 initial growth stage: crystalline NPs were formed after deposition of amorphous nanoparticles on the substrate and heating. [less ▲]

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See detailDC magnetron sputtering deposition of titanium oxide nanoparticles : influence
Dreesen, Laurent ULiege; Cecchet, Francesca; Lucas, Stéphane

Conference (2008, September)

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See detailOrientation and Order of Self-Assembled p-Benzenedimethanethiol Films on Pt(111) Obtained by Direct Adsorption and via Alkanethiol Displacement
Silien, Christophe; Dreesen, Laurent ULiege; Cecchet, Francesca et al

in Journal of Physical Chemistry C: Nanomaterials, Interfaces, and Hard Matter (2007), 111

Self-assembly of p-benzenedimethanethiol (p-BDMT) on Pt(111) has been investigated by high-resolution electron energy loss spectroscopy and sum-frequency generation spectroscopy. The molecules adsorb on ... [more ▼]

Self-assembly of p-benzenedimethanethiol (p-BDMT) on Pt(111) has been investigated by high-resolution electron energy loss spectroscopy and sum-frequency generation spectroscopy. The molecules adsorb on Pt-(111) like on gold, attached through one single thiol, leaving the second thiol moiety unreacted and exposed to the outer surface. Yet, the order of the p-BDMT self-assembled film is poor in comparison to alkanethiol SAMs. Displacement in solution of a preadsorbed pentanethiol (PTT) SAM by p-BDMT molecules proved to be a successful approach to achieve films of higher quality, where the size of the dithiol molecular domains tends toward the size measured for PTT. Nearly complete exchange is accomplished after 42 h. However, no significant exchange is observed when dodecanethiol replaces PTT. Displacing the alkanethiols by benzenethiol leads to the same conclusions, suggesting that the exchange processes are lessened as compared to gold. [less ▲]

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See detailAdsorption properties of decyl thiocyanate and decanethiol on platinum substrates studied by sum-frequency generation spectroscopy
Sartenaer, Yannick; Dreesen, Laurent ULiege; Humbert, Christophe et al

in Surface Science (2007), 601

Recently, thiocyanate groups were successfully used as precursors for thiolate assemblies. Indeed, they provide a useful alternative to thiol groups for self-assembly on metallic substrates. In order to ... [more ▼]

Recently, thiocyanate groups were successfully used as precursors for thiolate assemblies. Indeed, they provide a useful alternative to thiol groups for self-assembly on metallic substrates. In order to check the adsorption properties and the quality of the thiocyanate-based self-assembled monolayers (SAMs), we use sum-frequency generation spectroscopy (SFG) and apply it to characterize the ad-layers of two similar molecules: decanethiol (DT) and decyl thiocyanate (DTCN) adsorbed on platinum surfaces. By comparing the SFG signals of the methyl and methylene vibration modes, we show that DTCN SAMs are less ordered than DT ones. These effects are related to the SAMs quality which depends on the molecular packing as highlighted by scanning tunnelling microscopy and X-ray photoelectron spectroscopy. [less ▲]

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See detailDevelopment of an all-solid-state pulsed picosecond laser system for nonlinear spectroscopy
Mani, A. A.; Jazmati, A. K.; Zidan, M. D. et al

in Physical Chemical News (2007), 36

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See detailSum-frequency generation spectroscopy of DNA monolayers
Sartenaer, Yannick; Tourillon, Gérard; Dreesen, Laurent ULiege et al

in Biosensors & Bioelectronics (2007), 22

The anchoring of thiolated single-strandedDNA(HS-ssDNA) monolayers onto platinum substrates was investigated by sum-frequency generation spectroscopy. Different buffer solutions were used for the ... [more ▼]

The anchoring of thiolated single-strandedDNA(HS-ssDNA) monolayers onto platinum substrates was investigated by sum-frequency generation spectroscopy. Different buffer solutions were used for the preparation of the adlayers. Vibrational fingerprints in the 2700–3100 cm−1 spectral range showed the intercalation of Tris/EDTA (TE) buffer molecules within the HS-ssDNA self-assembled monolayer. Buffer contribution to SFG can be quenched either by using SFG inactive molecules like KH2PO4/K2HPO4/NaCl (PBS) or by repeated rinsing of the DNA layer with pure water. Comparing the SFG spectra of HS-ssDNA and mercaptohexanol (MCH), which had been self-assembled onto the same substrate, enabled us to infer ordering of the anchor arms and strong disordering of the DNA strands of HS-ssDNA monolayers self-assembled on platinum. [less ▲]

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See detailThe interaction between a protein and a ligand molecule studied by linear and non-linear optical spectroscopies
Dreesen, Laurent ULiege; Méthivier, C.; Sartenaer, Y. et al

in Physical Chemical News (2007), 36

We use infrared reflection-adsorption (IRRAS) and sum-frequency generation (SFG) spectroscopies to study the interaction between a protein (avidin) and a ligand molecule (biocytin or its thiol derivative ... [more ▼]

We use infrared reflection-adsorption (IRRAS) and sum-frequency generation (SFG) spectroscopies to study the interaction between a protein (avidin) and a ligand molecule (biocytin or its thiol derivative) adsorbed on metals (Au and Ag) and insulators (CaF2 in two separate spectral ranges 2800-3500 cm-1 and 1400-1800 cm-1, respectively. No specific interaction is detected by SFG when the measurements are carried out on metals although IRRAS measurements attest the presence of a protein ad-layer. This is explained by the disordered character of the adsorbed film of avidin which is therefore SFG inactive. When the experiments are performed on an insulating substrate, no change of the biocytin/CaF2 SFG spectrum is detected in the low spectral range (1400-1800 cm-1) after immersion in an avidin solution in contrast with what was clearly demonstrated in the 2800-3500 cm-1 spectral range. Finally, we report, for the first time, the observation by SFG of the CH2 scissor vibration mode at 1465 cm-1. [less ▲]

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See detailTotal internal reflection sum-frequency generation spectroscopy and dense gold nanoparticles monolayer: a route for probing adsorbed molecules
Tourillon, Gérard; Dreesen, Laurent ULiege; Volcke, Cédric et al

in Nanotechnology (2007), 18

We show that sum-frequency generation spectroscopy performed in the total internal reflection configuration (TIR–SFG) combined with a dense gold nanoparticles monolayer allows us to study, with an ... [more ▼]

We show that sum-frequency generation spectroscopy performed in the total internal reflection configuration (TIR–SFG) combined with a dense gold nanoparticles monolayer allows us to study, with an excellent signal to noise ratio and high signal to background ratio, the conformation of adsorbed molecules. Dodecanethiol (DDT) was used as probe molecules in order to assess the potentialities of the approach. An enhancement of more than one order of magnitude of the SFG signals arising from the adsorbed species is observed with the TIR geometry compared to the external reflection one while the SFG non-resonant contribution remains the same for both configurations. Although further work is required to fully understand the origin of the SFG process on nanoparticles, our work opens new possibilities for studying nanostructures. [less ▲]

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