References of "Detilleux, Johann"
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See detailA hidden Markov model to predict early mastitis from test-day somatic cell scores
Detilleux, Johann ULg

in Animal (2011), 5(2), 175-181

In many countries, high somatic cell scores (SCS) in milk are used as an indicator for mastitis because they are collected on a routine basis. However, individual test-day SCS are not very accurate in ... [more ▼]

In many countries, high somatic cell scores (SCS) in milk are used as an indicator for mastitis because they are collected on a routine basis. However, individual test-day SCS are not very accurate in identifying infected cows. Mathematical models may improve the accuracy of the biological marker by making better use of the information contained in the available data. Here, a simple hidden Markov model (HMM) is described mathematically and applied to SCS recorded monthly on cows with or without clinical mastitis to evaluate its accuracy in estimating parameters (mean, variance and transition probabilities) under healthy or diseased states. The SCS means were estimated at 1.96 (s.d.50.16) and 4.73 (s.d.50.71) for the hidden healthy and infected states, and the common variance at 0.83 (s.d.50.11). The probability of remaining uninfected, recovering from infection, getting newly infected and remaining infected between consecutive test days was estimated at 78.84%, 60.49%, 11.70% and 15%, respectively. Three different health-related states were compared: clinical stages observed by farmers, subclinical cases defined for somatic cell counts below or above 250 000 cells/ml and infected stages obtained from the HMM. The results showed that HMM identifies infected cows before the appearance of clinical and subclinical signs, which may critically improve the power of the studies on the genetic determinants of SCS and reduce biases in predicting breeding values for SCS. [less ▲]

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See detailSurgical hand antisepsis in veterinary practice: evaluation of soap scrubs and alcohol based rub techniques
Verwilghen, Denis ULg; Mainil, Jacques ULg; Mastrocicco, Emilie et al

in Veterinary Journal (2011), 190(3), 372-377

Recent evidence shows hydro-alcoholic solutions are more efficient than traditional medicinated soaps in the presurgical hand antisepsis of human surgeons. However, little veterinary literature is ... [more ▼]

Recent evidence shows hydro-alcoholic solutions are more efficient than traditional medicinated soaps in the presurgical hand antisepsis of human surgeons. However, little veterinary literature is available on the subject. The aims of this study were to compare the efficiency of medicinated soaps and a hydro-alcoholic solution prior to surgery using an in use testing method in a veterinary setting. A preliminary trial was performed that compared the mean Log10 number of bacterial colony forming units (CFU) and the reduction factors (RF) between 2 five-minute hand-scrubbing sessions using different soaps (povidone iodine (PVP) and chlorhexidine gluconate (CHX)) and the 1.5-min application of a hydro-alcoholic rub (Sterillium). A clinical in use trial comparing mean log10 number of bacterial CFU’s and RF for Sterillium and CHX soap was performed in a surgical set-up. Sampling was performed using finger printing on agar plates. Sterillium and CHX had a similar immediate effect, although the sustained effect was significantly better for Sterillium. PVP showed a significantly lower immediate and sustained effect. Sterillium showed good efficiency in the clinical trial. This study shows that, as previously reported for human medicine, Sterillium can safely be considered as an attractive alternative method for surgical hand antisepsis in a veterinary surgical setting. [less ▲]

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See detailTrend of hip fracture incidence in Belgium between 2000 and 2007 and future projections
Hiligsmann, Mickaël ULg; Bruyère, Olivier ULg; Detilleux, Johann ULg et al

in Value in Health (2010, November), 13(7), 303

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See detailAssociation between Myeloperoxidase concentration in equine frozen semen and post thawing parameters
Ponthier, Jérôme ULg; Franck, Thierry ULg; Detilleux, Johann ULg et al

in Reproduction in Domestic Animals (2010), 45(5), 811-816

Despite improvement of techniques, semen of 20% of stallions remains unfreezable. Recent studies focused on impact of reactive oxygen species and oxidant enzymes on semen characteristics. Myeloperoxidase ... [more ▼]

Despite improvement of techniques, semen of 20% of stallions remains unfreezable. Recent studies focused on impact of reactive oxygen species and oxidant enzymes on semen characteristics. Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in and released by neutrophils during degranulation or after cell lysis. It is responsible for the formation of hypochlorous acid, a strong oxidant agent which could damage spermatozoa. The aim of this study was to determine the relation between MPO concentration and characteristics of frozen semen from stallions. Thirty five straws from different stallions were analyzed. Post-thawing spermatozoal concentration, progressive and total motility were determined by CASA. Freezability was determined according to post-thawing progressive motility (over or under 15%). Percent of alive spermatozoa and abnormal forms were determined after Eosin-Nigrosin and Diff-Quick® staining respectively. Post-thawing MPO concentration was measured by ELISA. Our study shows that frozen thawed semen contains large amounts of free MPO. We also observed that post-thawing MPO ELISA assay can be used as an indicator of equine semen freezability. High MPO concentration samples showed lower total and progressive motility. A higher proportion of abnormal head shape associated with acrosome reaction was observed in our late examinations of the high concentration MPO group. Our results show that MPO adversely affects total and progressive motility of equine semen. A negative correlation between normal motile forms and MPO concentration was also observed. The effect of MPO on dead or abnormal forms remains to be precised. [less ▲]

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See detailRelationship between changes in bone mineral density or bone turnover markers and vertebral fracture incidence in patients treated with bazedoxifene
Bruyère, Olivier ULg; Detilleux, Johann ULg; Akadi, Chines et al

in Arthritis and Rheumatism (2010, October), 62(10), 406-407

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See detailEmergence of coagulase negative staphylococci in Southern Belgium - Facts and Figures
Theron, Léonard ULg; Bughin, Jean; Detilleux, Johann ULg et al

Conference (2010, September 16)

Since the early 1990s in Wallonia, coagulase negative staphylococci (CNS) have been identified as mastitis agent, both clinical and subclinical. As there epidemiological cluster has not yet been clearly ... [more ▼]

Since the early 1990s in Wallonia, coagulase negative staphylococci (CNS) have been identified as mastitis agent, both clinical and subclinical. As there epidemiological cluster has not yet been clearly classified between environmental and contagious pathogen, several questions subsists toward this class of mastitis etiological agents. From the late 1990s were the CNS represented 12% of infected quarters to 23% of all positive identifications in 2008, the CNS have taken a large part of the clinical cases of mastitis. Comparatively, CNS in Flanders represents only 7% of clinical cases identifications and 24% of subclinical cases. They still represent low inflammation pathogens, as for the average somatic cell count (SCC) of a CNS infection is 450.000 cells/ml (DETILLEUX, J. 1999. Preventive Veterinary Medicine, 41, 75-88.) but they represent for sure a source of treatment events in farm as for they provoke clinical mastitis. So, however they are seen as minor pathogens of a lesser matter regarding milk production drop, they still can imply a treatment a loss of discarded milk. Whether or not they should be treated could be approached through the Mammipack® project results. 124 common clinical mastitis events where sampled for bacteriology at day 0 and day 21 (Fig. 1). Farmer were asked to qualify the severity of the mastitis whether it had abnormal milk (score 1); abnormal quarter (score 2) or hyperthermia/anorexia (score 3). Also, they recorded the treatment used and we collected the SCC of each cow recorded, 2 months before the clinical signs until 3 months after. We decided to define as “chronic cases” cows showing SCC > 250.000 cells/ml the month before the clinical event and as “cured” cows showing SCC < 250.000 cells/ml the month after the event or the next one if the mastitis took place less than 15 days before test day. We could show that CNS infections still account for the least severe cases (Fig. 2), except for S. simulans, S. saprophyticus and S. hominis that shown some quarter indurations. While only 10% of clinical infection does remain clinically uncured to the farmer, figure 3 explains how 50% of CNS infections in our essay remained globally cured (Bacteriological and tissular), even if 40% of their treatments shown to be inadequate. Also, only one infection could be referred as a chronic case, all the others were acute cases. Though this study recorded a small amount of cases, yet we did not find any results differing from known characteristics of CNS infections. One interesting facts is the quite easy cure of the infected quarters given the poor quality of treatment. [less ▲]

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See detailRelationship between 3-month changes in biochemical markers of bone remodelling and changes in bone mineral density and fracture incidence in patients treated with strontium ranelate for 3 years.
Bruyère, Olivier ULg; Collette, Julien ULg; Rizzoli, R. et al

in Osteoporosis International (2010), 21

From two randomised controlled trials, it is shown that 3-month changes in biochemical markers of bone formation (bone-specific alkaline phosphatase and C-terminal propeptide of type I procollagen) were ... [more ▼]

From two randomised controlled trials, it is shown that 3-month changes in biochemical markers of bone formation (bone-specific alkaline phosphatase and C-terminal propeptide of type I procollagen) were associated with 3-year bone mineral density (BMD) changes, but not fracture incidence in patients treated with strontium ranelate. INTRODUCTION: The purpose of this study was to assess if short-term change in biochemical markers of bone remodelling is associated with long-term BMD change and fracture incidence observed during treatment with strontium ranelate. METHODS: From the SOTI and TROPOS trials, bone-specific alkaline phosphatase (BALP), C-terminal propeptide of type I procollagen (PICP), serum C-terminal telopeptides (S-CTX) and urine N-terminal telopeptides of type I collagen (U-NTX) were assessed at baseline and after 3 months. RESULTS: Two thousand three hundred seventy-three women were included in this study. Multiple regression analysis showed that 3-month changes in PICP and BALP but not s-CTX I nor s-NTX I were significantly (p < 0.001) associated with 3-year BMD changes at the lumbar spine and the femoral neck. Changes in s-CTX I, PICP and BALP were significantly associated with change in total proximal femur BMD. Changes in biochemical markers explain less than 8% of the BMD changes. The 3-month changes in BALP, PICP s-CTX I and s-NTX I were not significantly associated with fracture incidence. CONCLUSIONS: Short-term changes in biochemical markers of bone formation are associated with future BMD changes in patients treated with strontium ranelate, suggesting a bone-forming activity of this treatment, but are not appropriate to monitor the efficacy of strontium ranelate at the individual level. [less ▲]

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See detailEvaluation of low-molecular-weight heparin for the prevention of equine laminitis after colic surgery
de la Rebière de Pouyade, Geoffroy ULg; Grulke, Sigrid ULg; Detilleux, Johann ULg et al

in Journal of Veterinary Emergency and Critical Care (2009), 19(1), 113-119

Objectives – The aim of this study is to describe the prevalence of postoperative laminitis in colic cases and to determine if low-molecular-weight heparin (LMWH) is effective in preventing this ... [more ▼]

Objectives – The aim of this study is to describe the prevalence of postoperative laminitis in colic cases and to determine if low-molecular-weight heparin (LMWH) is effective in preventing this complication. Design – Retrospective clinical study. Animals – Client-owned horses. Interventions – SC administration of enoxaparin during the postoperative period. Measurements and Main Results – Medical records of 360 horses undergoing surgery for colic and surviving at least 3 days were evaluated. Fifty-six horses admitted before 1995 did not receive LMWH (control group) and 304 admitted after 1995 received LMWH as a prophylaxis for laminitis (treatment group). Three grades of severity were defined for laminitis. Prevalence and severity of laminitis were compared between the 2 groups. Several parameters recorded on admission (sex, age, breed, site and nature of the disease, heart rate, PCV, gravity score, and shock score) and the administration of LMWH were tested as risk factors in the development of laminitis in a logistic regression procedure. Prevalence and grade of laminitis were significantly lower in the treatment group. Only the absence of LMWH was recognized as a significant risk factor in the logistic regression model. Conclusions – The administration of LMWH appears to be effective in the prophylaxis of laminitis following colic surgery and may be useful in the postoperative management of these horses. [less ▲]

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See detailRelationship between biochemical markers and radiographic scores in the evaluation of the osteoarticular status of Warmblood stallions
Verwilghen, Denis ULg; Busoni, Valeria ULg; Gangl, Monika et al

in Research in Veterinary Science (2009), 87(2), 319-28

Establishing the osteoarticular status of the horse is often performed by means of radiological screening of the animals. Widespread blood sampling could reveal to be an interesting alternative to this ... [more ▼]

Establishing the osteoarticular status of the horse is often performed by means of radiological screening of the animals. Widespread blood sampling could reveal to be an interesting alternative to this procedure which is time consuming and sometimes technically difficult. The aim of this study was to investigate the relationship between the radiological status of the horses and the levels of biochemical markers of cartilage degradation and synovial inflammation. A specific radiological scoring and classification system was therefore developed and applied on 63 stallions presented for studbook admission. Additionally, groups of horses were established according to the occurrence of osteochondrosis, degenerative joint disease and distal interphalangeal joint effusion. Insulin growth factor-I, myeloperoxidases, Coll2-1 and Coll2-1NO(2) were used as blood markers. The combination of the blood parameters did not seem to correlate with the used scoring system. Coll2-1NO(2) levels however tended to increase with poorer radiological class and this could therefore potentially be a useful predictor of the osteoarticular status in the horse. Coll2-1 levels were significantly higher in the degenerative joint disease group. A high percentage of horses with distal interphalangeal joint effusion was present in this study and was associated with decreased IGF-I and increased Coll2-1 levels. [less ▲]

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See detailReproductive Performance of the Ardennaise Chicken Breed under Traditional and Modern Breeding Management Systems
Larivière, Jean-Marc ULg; Michaux, Charles ULg; Farnir, Frédéric ULg et al

in International Journal of Poultry Sciences (2009), 8(5), 446-451

Enhancing the use of traditional chicken breeds is necessary to insure long-term conservation of global genetic diversity in poultry. A logical start in this process is to evaluate performance. The ... [more ▼]

Enhancing the use of traditional chicken breeds is necessary to insure long-term conservation of global genetic diversity in poultry. A logical start in this process is to evaluate performance. The objective of this study was to estimate reproductive traits in the Ardennaise chicken breed under traditional and modern management systems. Reproductive performance indicated lighting programs [Natural (NAT) or Artificial (ART) day length] were highly significant for egg weight, hatchability (p<0.001), early and mid-term embryonic mortality (p<0.01). Feeding treatments [ad libitum (AL) or mild Feed-restriction (FR)] varied greatly with egg weight, hatchability, fertility (p<0.001) and late embryonic mortality (p<0.05). Most precocious age at first egg (23 weeks), longest duration of laying period (>27 weeks), utmost maximum production peak (56.67%), largest egg number (70.13 eggs/hen) and number of chicks hatched (24.10 chicks/hen) were performed under ART-AL. Heaviest egg weight (53.21g), highest fertility (70.20%), greatest hatchability (55.58%), highest liveability during rearing (95.74%) and lay (100%), lowest early (3.51%) and mid-term embryonic mortality (1.17%) were achieved under NAT-AL. To summarize, most of traits studied in the Ardennaise chicken were moderate when compared to those expected by broiler breeders. [less ▲]

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See detailLoss of hip bone mineral density over time is associated with spine and hip fracture incidence in osteoporotic postmenopausal women.
Bruyère, Olivier ULg; Varela, A. R.; Adami, S. et al

in European journal of epidemiology (2009), 24

The objective of the study assess the relationship between bone mineral density (BMD) loss over time and fracture incidence in postmenopausal women. This is a posthoc analysis that includes women from the ... [more ▼]

The objective of the study assess the relationship between bone mineral density (BMD) loss over time and fracture incidence in postmenopausal women. This is a posthoc analysis that includes women from the placebo group of two large randomized controlled trials having assessed the efficacy of a new anti-osteoporotic drug. BMD was assessed every 6 months during 3 years at the lumbar spine, the femoral neck and the total proximal femur. Vertebral fractures were assessed using a semiquantitative method. Hip fractures were based on written documentation. All patients received calcium and vitamin D. In the present study that included 1,775 patients (with complete data at baseline and after 3 years), the logistic regression analysis, adjusted for covariates, showed that 3-year change in lumbar BMD was not statistically associated with the new vertebral fractures after 3 years. However, femoral neck and total proximal femur BMD changes was statistically correlated with the incidence of new vertebral fractures (P < 0.001). When considering change in BMD after the first year of follow-up, a decrease in total proximal femur BMD was statistically associated with an increase in the incidence of new vertebral fractures during the last 2 years of follow-up (P = 0.048). The 3-year change in femoral neck and total proximal BMD was statistically correlated with the incidence of hip and fragility fracture after 3 years (all P < 0.001). In this elderly osteoporotic population receiving calcium and vitamin D, a decrease in hip BMD after 1 or 3 year of follow-up, is associated with an increased risk of fracture incidence. However, spine BMD changes do not influence vertebral fracture incidence. [less ▲]

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See detailCardiac Power Output during Dobutamine Stress
Sandersen, Charlotte ULg; Mc Entee, Kathleen ULg; Deleuze, Stefan ULg et al

in Journal of Equine Veterinary Science (2009)

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See detailGenetic factors affecting susceptibility to udder pathogens
Detilleux, Johann ULg

in Veterinary Microbiology (2009), 134(1-2), 157-164

Many studies have identified genetic factors underlying resistance or susceptibility to mastitis in dairy cows and heifers. Some authors focused on polygenic variation while others searched for genes and ... [more ▼]

Many studies have identified genetic factors underlying resistance or susceptibility to mastitis in dairy cows and heifers. Some authors focused on polygenic variation while others searched for genes and/or quantitative trait loci with major effects on mastitis. Classical traits related to mastitis include somatic cell counts, electrical conductivity and clinical cases of the disease. With the development of automatic milking devices and '-omics' technologies, new traits are considered, such as acute phase proteins, immunological assays, and milk flow patterns, and new biological pathways are discovered, for example the role of mammary epithelium and the nervous system. The usefulness of these traits for the identification of resistant cows is discussed in relation to the biological mechanisms underlying the development of the disease. In addition, the utility of these traits for genetic improvement is reviewed. Finally, the problem of durability in resistance is addressed, including co-evolution and the cost of resistance. [less ▲]

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See detailInfluence de l'apport énergétique et de l'hibernation sur la croissance des tortues terrestres
Diez, Marianne ULg; Justet, Delphine; Detilleux, Johann ULg et al

in 15th FECAVA Eurocongress - Proceedings (2009)

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See detailEffects of different levels of energy allowances and of hibernation on growth in hatchling Testudo hermanni boettgeri (Mojsisovic, 1889)
Diez, Marianne ULg; Vanstrazeele, Boris; Justet, Delphine et al

in Clauss, M.; Fidgett, A.; Hatt, J. M. (Eds.) et al Zoo Animal Nutrition IV (2009)

Cet article présente des données de croissance de Testudo nouveaux nés dont les apports alimentaires et l'hibernation ont été contrôlés.

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