Somatic Cell (Neutrophil) Counts in the War Against Staphylococcus aureus: Predator-Prey Models at the RescueDetilleux, Johann ![]() in Journal of Dairy Science (2004), 87(11), 3716-3724 To address the question of whether a minimum concentration of blood neutrophils is necessary to decrease Staphylococcus aureus concentration in mastitic milk, literature was searched for studies in which ... [more ▼] To address the question of whether a minimum concentration of blood neutrophils is necessary to decrease Staphylococcus aureus concentration in mastitic milk, literature was searched for studies in which neutrophils were incubated with Staph. aureus. Different mathematical models that describe the changes in Staph. aureus population as a function of neutrophilic concentrations were applied to the collected data. The best fitted model established (1) that the rate of bacterial killing depended on the ratio of neutrophils to bacteria with neutrophilic attack rate accelerating at first before decelerating as the ratio increases, and (2) that neutro-phil concentration should be within a limited range to trigger a decline in the bacterial population. Outcomes of this model are supported by what is known about neutrophilic functions and laboratory findings in bovine and human neutrophils. These results may be of assistance in setting selection goals for a better resilience to Staph. aureus mastitis in dairy cattle. Indeed, an optimal neutrophilic concentration appears to exist for successful clearance of Staph. aureus infection, which is neither the lowest nor the highest one. [less ▲] Detailed reference viewed: 12 (2 ULg) Ad libitum feeding following ovariectomy in female Beagle dogs: effect on maintenance energy requirement and on blood metabolites.; Detilleux, Johann ; Cuvelier, Christine et alin Journal of Animal Physiology & Animal Nutrition (2004), 88(3-4), 117-21 The aim of this study was (i) to evaluate the effect of ovariectomy on daily energy requirement in Beagle dogs, and (ii) to evaluate the effects of ad libitum feeding with a high energy diet on energy ... [more ▼] The aim of this study was (i) to evaluate the effect of ovariectomy on daily energy requirement in Beagle dogs, and (ii) to evaluate the effects of ad libitum feeding with a high energy diet on energy consumption, body weight gain and blood metabolites in these spayed dogs. Four young adult female Beagle dogs were used. Ovariectomy induced a significant decrease of daily energy requirement in dogs. Ad libitum feeding, initiated 6 months after spaying, induced a significant increase in consumption in spayed dogs. This overconsumption was greatest during the first month of ad libitum feeding but continued for the entire 4 months of this period. When fed ad libitum, dogs gained excess body weight without significant effect on plasma cholesterol, triglycerides, glucose and insulin, at short term. These data suggest that energy intake should be strictly controlled to avoid excess weight gain in spayed dogs. [less ▲] Detailed reference viewed: 49 (1 ULg) Mixture model for inferring susceptibility to mastitis in dairy cattle: a procedure for likelihood-based inference; ; et al in Genetics, Selection, Evolution [=GSE] (2004), 36(1, JAN-FEB), 3-27 A Gaussian mixture model with a finite number of components and correlated random effects is described. The ultimate objective is to model somatic cell count information in dairy cattle and to develop ... [more ▼] A Gaussian mixture model with a finite number of components and correlated random effects is described. The ultimate objective is to model somatic cell count information in dairy cattle and to develop criteria for genetic selection against mastitis, an important udder disease. Parameter estimation is by maximum likelihood or by an extension of restricted maximum likelihood. A Monte Carlo expectation- maximization algorithm is used for this purpose. The expectation step is carried out using Gibbs sampling, whereas the maximization step is deterministic. Ranking rules based on the conditional probability of membership in a putative group of uninfected animals, given the somatic cell information, are discussed. Several extensions of the model are suggested. [less ▲] Detailed reference viewed: 7 (0 ULg) Stress echocardiography in horses: comparison of cardiac output during incremental dobutamine infusion in atropinised and non-atropinised poniesSandersen, Charlotte ; Detilleux, Johann ; Delguste, Catherine et alin Proceedings of the 42d Annual Congress of the British Equine Veterinairy Association (2003, September) Detailed reference viewed: 10 (3 ULg) Clinicopathological data in equids suffering from ante-hepatic, hepatic and post-hepatic diseasesAmory, Hélène ; ; Sandersen, Charlotte et alin Proceedings of the 42th Annual Congress of the British Equine Veterinary Association (BEVA) (2003) Detailed reference viewed: 4 (0 ULg) Prognostic value of clinical signs and blood parameters in equids suffering from acute hepatic insufficiency: a retrospective study on 31 casesAmory, Hélène ; ; Sandersen, Charlotte et alin Proceedings of the 21st ACVIM annual meeting, Charlotte, North Carolina (2003) Detailed reference viewed: 8 (1 ULg) Prognostic value of clinical signs and blood parameters in equids suffering from acute hepatic insufficiency: a retrospective study on 31 casesAmory, Hélène ; ; Sandersen, Charlotte et alin Journal of Veterinary Internal Medicine (2003) Detailed reference viewed: 3 (0 ULg) Rhinitis/Bronchopneumonia syndrome in Irish Wolfhounds.Clercx, Cécile ; ; Peeters, Dominique et alin Journal of Veterinary Internal Medicine (2003), 17(6), 843-9 This study describes the clinical, immunologic, genetic, and pathologic features of Irish Wolfhounds with rhinitis/bronchopneumonia syndrome. The dogs examined were from Belgium, The Netherlands, UK ... [more ▼] This study describes the clinical, immunologic, genetic, and pathologic features of Irish Wolfhounds with rhinitis/bronchopneumonia syndrome. The dogs examined were from Belgium, The Netherlands, UK, Canada, Germany, and Switzerland. Signs included transient to persistent mucoid or mucopurulent rhinorrhea, cough, and respiratory dyspnea. Radiographic, rhinoscopic, and bronchoscopic findings were variable. Analysis of ciliary ultrastructure was performed in 5 affected dogs, but no characteristic primary ciliary defects (primary ciliary dyskinesia) were detected. Serum and bronchoalveolar lavage fluid (BALF) concentrations of IgA, IgG, and IgM were determined in some affected dogs and clinically normal Irish Wolfhounds. Serum IgA concentration was below the reference range in 5 of 8 affected dogs tested, whereas BALF IgA concentration was above the normal range in 2 affected adult dogs. The CD4 to CD8 lymphocyte subset ratio (CD4:CD8) in peripheral blood was tested in 3 affected dogs and was within the normal range. BALF CD4:CD8 was tested in 1 affected dog and was higher than the normal range. Decreased neutrophil phagocytosis was observed in 1 of the 4 dogs tested. Analysis of pedigrees of the Belgian, Canadian, German, and Swiss dogs revealed common ancestry, suggesting a heritable syndrome. [less ▲] Detailed reference viewed: 45 (2 ULg) Use of atropine to inhibit vagally mediated baroreceptor reflex in horses undergoing dobutamine stress testSandersen, Charlotte ; Detilleux, Johann ; Delguste, Catherine et alin Pflügers Archiv : European Journal of Physiology (2003), 446(5), 4 Detailed reference viewed: 5 (2 ULg) Defense of the bovine mammary gland by polymorphonuclear neutrophil leukocytes; ; et al in Journal of Mammary Gland Biology and Neoplasia (2002), 7(2), 109-121 The primary phagocytic cells of the bovine mammary gland, polymorphonuclear neutrophil leukocytes (PMN), and macrophages, comprise the first line of defense against invading pathogens. In the normal ... [more ▼] The primary phagocytic cells of the bovine mammary gland, polymorphonuclear neutrophil leukocytes (PMN), and macrophages, comprise the first line of defense against invading pathogens. In the normal healthy mammary gland, macrophages predominate and act as sentinels to invading mastitis-causing pathogens. Once invaders are detected, macrophages, and possibly mammary epithelial cells, release chemoattractants that direct migration of PMN into the area. In the mammary gland, protection is only effective if rapid influx of PMN from the circulation and subsequent phagocytosis and killing of bacteria occur. The second line of defense against infection consists of a network of memory cells and immunoglobulins that interact with the first line of defense. To minimize mammary tissue damage caused by bacterial toxins and oxidative products released by PMN, elimination of invading bacteria must proceed quickly. Therefore, the inflammatory response needs to be regulated. Hormones, metabolites, and acute phase proteins act to influence the outcome of mastitis, especially around parturition. The number of circulating PMN in cows during early lactation is highly heritable and closely related to susceptibility to clinical mastitis at this time. Advances in molecular biology are making available the tools, techniques, and products to study and modulate host-pathogen interactions. For example, the cloning and expression of proteins such as recombinant bovine soluble (rbos) CD (cluster of differentiation) 14 antigens, may provide ways of minimizing damaging effects of endotoxin during acute coliform mastitis. Soluble CD14 binds and neutralizes lipopolysacharide (LPS) and causes local recruitment of PMN after binding of CD14-LPS complexes to mammary epithelial cells. Development of transgenic animals that express rbosCD14 in their milk could prevent infection by Gram-negative pathogens. [less ▲] Detailed reference viewed: 45 (4 ULg) The assessment of third metacarpal bone speed of sound measurements in young exercising thorougbreds; ; Detilleux, Johann et alin Proceedings of the European Society of the Veterinary Society (ESVOT) (2002) Detailed reference viewed: 2 (0 ULg) Speed of sound measurements in the evaluation of bone properties in HolsteinSandersen, Charlotte ; Guyot, Hugues ; Vandeputte, Sébastien et alin Proceedings of the XXII World Buiatrics Congress (2002) Detailed reference viewed: 6 (2 ULg) Einsatz der quantitativen Ultrasonometrie in der Knochendiagnostik beim Pferd; ; Detilleux, Johann et alPoster (2002) Detailed reference viewed: 3 (0 ULg) Approche clinique des ictères et des suspicions d’insuffisance hépatique aiguë chez le cheval : une étude rétrospective sur 40 casAmory, Hélène ; Sandersen, Charlotte ; Delguste, Catherine et alin Proceedings of the Annual meeting of the French Equine Veterinary Association (AVEF) (2002) Detailed reference viewed: 26 (1 ULg) Genetic factors affecting susceptibility to udder pathogensDetilleux, Johann ![]() in Veterinary Immunology and Immunopathology (2002), 88(3-4), 103-110 Bovine mastitis remains the most costly disease in dairy cattle. Breeding for resistance to udder pathogens has been proposed as a complementary tool to therapeutic and prophylactic measures not totally ... [more ▼] Bovine mastitis remains the most costly disease in dairy cattle. Breeding for resistance to udder pathogens has been proposed as a complementary tool to therapeutic and prophylactic measures not totally effective against the disease. This paper reviews factors affecting cows’ susceptibility to pathogens at the animal, cellular/hormonal and DNA levels. Such factors will be useful in achieving genetic improvement for resistance only if they have desirable properties at the genetic and immunological levels. Because such properties are not always of significant magnitude, further research is necessary to identify characteristics of resistance in cows, considering the constant and complex interactions that occur between hosts and pathogens. [less ▲] Detailed reference viewed: 10 (1 ULg) An Immunologic investigation of canine eosinophilic bronchopneumopathy.Clercx, Cécile ; Peeters, Dominique ; et alin Journal of Veterinary Internal Medicine (2002), 16 Detailed reference viewed: 8 (3 ULg) A role for the Clostridium perfringens beta 2 toxin in bovine enterotoxaemia?; Daube, Georges ; Jauniaux, Thierry et alin Veterinary Microbiology (2002), 86(3), 191-202 Non-enterotoxigenic type A Clostridium perfringens are associated with bovine enterotoxaemia, but the alpha toxin is not regarded as responsible for the production of typical lesions of necrotic and ... [more ▼] Non-enterotoxigenic type A Clostridium perfringens are associated with bovine enterotoxaemia, but the alpha toxin is not regarded as responsible for the production of typical lesions of necrotic and haemorrhagic enteritis. The purpose of this study was to investigate the putative role of the more recently described beta2 toxin. Seven hundred and fourteen non-enterotoxigenic type A C. perfringens isolated from 133 calves with lesions of enterotoxaemia and high clostridial cell counts (study population) and 386 isolated from a control population of 87 calves were tested by a colony hybridisation assay for the beta2 toxin. Two hundred and eighteen (31%) C perfringens isolated from 83 calves (62%) of the study population and 113 (29%) C. perfringens isolated from 51 calves (59%) of the control population tested positive with the beta2 probe. Pure and mixed cultures of four C perfringens (one alpha+beta2+, one alpha+enterotoxin-1 and two alpha+) were tested in the ligated loop assay in one calf. Macroscopic haemorrhages of the intestinal wall, necrosis and haemorrhages of the intestinal content, and microscopic lesions of necrosis and polymorphonuclear and mononuclear cell infiltration of the intestinal villi were more pronounced in loops inoculated with the a and beta2-toxigenic C. perfringens isolate. These results suggest in vivo synergistic role of the alpha and beta2 toxins in the production of necrotic and haemorrhagic lesions of the small intestine in cases of bovine enterotoxaemia. However, isolation of beta2-toxigenic C. perfringens does not confirm the clinical diagnosis of bovine enterotoxaemia and a clostridial cell counts must still be performed. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲] Detailed reference viewed: 122 (25 ULg) Determination of a gravity and shock score for prognosis in equine surgical colicGrulke, Sigrid ; ; Detilleux, Johann et alin Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine (2001), 48(8), 465-73 A retrospective study evaluated 200 surgical colic cases. A gravity score (GS) based on four clinical parameters estimating intestinal obstruction (rectal palpation, borborygmi, abdominal distension, pain ... [more ▼] A retrospective study evaluated 200 surgical colic cases. A gravity score (GS) based on four clinical parameters estimating intestinal obstruction (rectal palpation, borborygmi, abdominal distension, pain) and classified into three categories was established and tested to determine if it could evaluate prognosis. A shock score (SS) based on six parameters was also attributed to each case. The overall survival rate was 54%. The statistical analysis showed a significant (P < 0.01) difference in the survival rate in the different categories of the GS, as well as in the categories of the SS. A multivariate logistic regression model showed that horses with GS 3 are 10.6 times more likely to die than those with GS 1. A model combining the two scores showed an odds ratio of 7.1 for GS 3 versus GS 1, and for SS 3 versus SS 1, the odds ratio was 7.2. [less ▲] Detailed reference viewed: 40 (12 ULg) Cross-Sectional Study of the Association between Pathological Conditions and Myxoma-Virus Seroprevalence in Intensive Rabbit Farms in EuropeMarlier, Didier ; ; Detilleux, Johann et alin Preventive Veterinary Medicine (2001), 48(1), 55-64 Myxomatosis is a major viral disease of the European rabbit (Oryctolagus cuniculus). Two forms of the disease (nodular and amyxomatous) exist. The clinical diagnosis of the nodular form is easily ... [more ▼] Myxomatosis is a major viral disease of the European rabbit (Oryctolagus cuniculus). Two forms of the disease (nodular and amyxomatous) exist. The clinical diagnosis of the nodular form is easily performed on the basis of typical skin lesions whereas that of amyxomatous forms must be based on virus isolation or detection of specific antibodies to myxoma virus (MV). The seroprevalence of MV was studied between March 1998 and February 1999 in 16 farms from three European countries considered free of myxomatosis on the basis of the absence of typical clinical signs. MV antibodies were detected by enzyme-linked immunosorbent assay (ELISA) (sensitivity 100%, specificity 90%) in all 16 farms; the seroprevalences corrected for test inaccuracy (95% confidence interval) were 55+/-7.7% and 37+/-6.1% for does and broilers, respectively. The association between herd sizes, types of rabbitries, and presence of recurrent respiratory or digestive troubles and seroprevalence of MV antibodies was tested in logistic multiple regressions. In all models, the seroprevalence of MV antibodies was significantly higher in herds (does and broilers) with recurrent respiratory or digestive troubles than in herds without these problems. The seroprevalence was also higher in herds (does and broilers) where animals were housed totally or partially in outdoors rabbitries than in totally enclosed rabbitries. The effect of herd sizes on the presence of MV antibodies was the same in does and broilers; intermediate sizes were at lower risk than the smaller and larger ones. [less ▲] Detailed reference viewed: 24 (1 ULg) Spirometric performance in Belgian Blue calves: I. Effects on economic losses due to the bovine respiratory disease complex.Bureau, Fabrice ; Detilleux, Johann ; et alin Journal of Animal Science (2001), 79(5), 1301-1304 The aim of this study was to determine whether high spirometric performances in calves are associated with low economic losses due to the bovine respiratory disease complex (BRDC). Five spirometric ... [more ▼] The aim of this study was to determine whether high spirometric performances in calves are associated with low economic losses due to the bovine respiratory disease complex (BRDC). Five spirometric variables (SV) were measured in 909 double-muscled Belgian Blue calves from 15 to 60 d of age. Afterward, calves were monitored for 6 mo to determine whether they developed BRDC and to determine the costs due to BRDC (i.e., medicine costs and veterinarians' fees, plus estimated financial losses due to mortality in case of death). To analyze the effects of spirometric performances on BRDC cost, a fixed linear model was used for each SV. In addition to SV, each model included the effects of sex, dam's parity, vaccination status, muscular development score, herd-period, and BW. Only herd-period and the maximal ventilation and the vital capacity had significant effects on costs due to BRDC, indicating that these two SV are major physiological determinants of economic losses associated with BRDC. Accordingly, it is assumed that an amelioration of maximal ventilation and vital capacity could result in increased resistance to BRDC in calves. [less ▲] Detailed reference viewed: 24 (2 ULg) |
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