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See detailThe Resistance to Freeze-Drying and to Storage Was Determined as the Cellular Ability to Recover Its Survival Rate and Acidification Activity
Coulibaly, Ibourahema ULg; Dubois-dauphin, Robin; Destain, Jacqueline ULg et al

in International Journal of Microbiology (2010), 2010(625239),

The protective effects of the fatty acid composition and membrane action of the acidification activity of two strains of Lactobacillus kept at 20◦C were studied. The addition of sorbitol, monosodium ... [more ▼]

The protective effects of the fatty acid composition and membrane action of the acidification activity of two strains of Lactobacillus kept at 20◦C were studied. The addition of sorbitol, monosodium glutamate and glycerol during storage is causing the decline of acidification and increased concentrations of unsaturated fatty acids observed in both strains. The addition of sorbitol and monosodium glutamate does not alter the fatty acid composition, whatever the strain, but increases the resistance to freeze-drying of L. plantarum CWBI-B1419 and improves survival during storage. The addition of these preservatives and decreased activity of acidification improves the ratio unsaturated. These results indicate that the survival during storage and freeze-drying resistance are closely related to the composition of membrane fatty acids. This behaviour can be interpreted as an adaptation of L. plantarum B1419-CWBI supplemented by cryoprotectant additives such as sorbitol or monosodium glutamate sorbitol and monosodium glutamate as an additive. L. plantarum CWBI-B1419 presents a greater adaptation to culture conditions than L. paracasei ssp. paracasei LMG9192T. [less ▲]

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See detailIsolation and characterisation of new spore-forming lactic acid bacteria with prospects of use in food fermentations and probiotic preparations
Bayane, Ali; Diawara, B.; Dubois Dauphin, Robin ULg et al

in African Journal of Microbiology Research [=AJMR] (2010), 4(11), 1016-1025

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See detailIntegrated “omics” approaches to investigate the chemical aspects of symbiosis in termites and potential application in ligno-cellulosic use.
Bauwens, Julien ULg; Matteotti, Christel ULg; Brognaux, Alison ULg et al

Scientific conference (2010, May 05)

Cellulosic bioethanol could be one of the solutions to satisfy the increasing demand in renewable energy. The most limitative problem is the efficiency of cellulose transformation into fermentable sugars ... [more ▼]

Cellulosic bioethanol could be one of the solutions to satisfy the increasing demand in renewable energy. The most limitative problem is the efficiency of cellulose transformation into fermentable sugars. Investigations to select new glycosyl hydrolases are an interesting approach that constitutes a potential opportunity to improve the valorization of lignocellulosic materials. Three major types of glycosyl hydolases are generally produced by organism’s that are able to efficiently use cellulosic compounds: the endoglucanases, the exoglucanases/cellobiohydrolases and the β-glucosidases. In the ability to transform lignocellulosic materials by animals, symbioses are generally observed with a range of micro-organisms including bacteria, protists and/or fungi that largely (or completely) contribute to the production of the needed enzymatic complexes. In termites, such active enzymes are produced in the insect digestive tract, by the termite insect itself or by symbiotic organisms. Within lower termites gut, such as in our model Reticulitermes santonensis (Feytaud), protists and bacteria are associated and involved in a complex symbiotic system. To investigate the respective role of the insect and different groups of symbionts, multidisciplinary “omics” approaches were here developed including proteomics (ESI-LC-MS-MS, 2D-Dige gel coupled with MALDI-TOF mass spectrometry for protein identification), genomics (with a metagenomic approach based on large cDNA bank construction), metabolomics (LC-MS stragety for carbohydrate degradation product characterization). Moreover, microorganism isolation was used to investigate and characterize glycosyl hydrolases diversity and activity in R. santonensis. The integration of this broad range of “omics” techniques allowed characterizing the role of symbionts in insects in a fundamental approach and to invtigate the chemical ecology of xylophagous insects but also corresponding to an efficient way to promote the selection of efficient enzymatic activities to potentially produce biofuels based on the use of existing lignocellulosic materials. [less ▲]

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See detailParticularities of Aphidius ervi embryo development and main limitations of its in vitro culture
Sabri, Ahmed ULg; Leroy, Pascal ULg; Hance, Thierry et al

Poster (2010, May 05)

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See detailLa production de bioéthanol à partir de biomasse lignocellulosique
Didderen, I.; Destain, Jacqueline ULg; Thonart, Philippe ULg

Article for general public (2010)

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See detailDetermination of phospholipids extracted from Tsukamurella paurometabola C-924 after freeze-drying and during the subsequent storage
Coulibaly, I.; Dauphin, R. D.; Destain, Jacqueline ULg et al

Scientific conference (2010)

The total lipids of Tsukamurella paurometabola C-924 were analyzed after freeze-drying. Seven individual lipids classes were identifiednamelyneutrallipids(NLs),fattyacids(FAs),phospholipids(PLs ... [more ▼]

The total lipids of Tsukamurella paurometabola C-924 were analyzed after freeze-drying. Seven individual lipids classes were identifiednamelyneutrallipids(NLs),fattyacids(FAs),phospholipids(PLs),sterolester(SEs),triglycerides(TGs),diglycerides (DGs) and monoglycerides (MGs). The principal fatty acids identified in most lipid classes were palmitic (C 16:0), palmitoleic (C 16:1), oleic (C 18:1), linoleic (C 18:2), and linolenic (C 18:3). PLs were the major constituents and accounted for 50-60% of the total lipids. PLs were fractionated. PLs of Tsukamurella paurometabola content phosphatidic acid (PA), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylcholine (PC), sphingomyelin (SM), lysophosphatidylcholine (LPC) and phosphatidylglycerol (PG). It was observed that PG had the highest proportion at most points relative to other PLs and was the predominant component of PLs (30%-56%). Evolution of individual rate was followed during storage at 20°C and 40°C with or without lithothamne400®, respectively. [less ▲]

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See detailIsolation, pure culture and characterization of Serratia symbiotica, the R-type of secondary endosymbionts of the black bean aphid Aphis fabae.
Sabri, Ahmed ULg; Leroy, Pascal ULg; Haubruge, Eric ULg et al

in International Journal of Systematic and Evolutionary Microbiology (2010)

An intracellular symbiotic bacterium was isolated from the flora of a natural clone of the black bean aphid Aphis fabae. The strain was able to grow freely in aerobic condition on a rich medium containing ... [more ▼]

An intracellular symbiotic bacterium was isolated from the flora of a natural clone of the black bean aphid Aphis fabae. The strain was able to grow freely in aerobic condition on a rich medium containing 1% of each of following substrates; dextrose, yeast extract and peptone of casein. Pure culture was achieved through the use of solid-phase culture on the same medium and was designated CWBI-2.3T. 16S ribosomal DNA sequence analysis revealed that CWBI-2.3T strain was a member of the gamma-3 subgroup of the class Proteobacteria, having high sequence identity (>99%) with the R-Type of secondary endosymbiont found in several aphid species Serratia symbiotica. As strain CWBI-2.3T (=LMG 25624T =DSM 23270T) was the first R-type symbiont to be isolated and characterized, we proposed to designate it as the type strain of Serratia symbiotica species. [less ▲]

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See detailHydrolysis of starch by sorghum malt for maltodextrin production
Ba, K.; Destain, Jacqueline ULg; Thonart, Philippe ULg

Scientific conference (2010)

Maltodextrin is a mixture of saccharides with a molecular weight between polysaccharides and oligosaccharides with DE lower than 20. Maltodextrin is more soluble in water than native starches, also is ... [more ▼]

Maltodextrin is a mixture of saccharides with a molecular weight between polysaccharides and oligosaccharides with DE lower than 20. Maltodextrin is more soluble in water than native starches, also is cheaper in comparison with other major edible hydrocolloids. Maltodextrin is obtained by moderate enzymatic or acidic hydrolysis of starch. The hydrolysis of starch, catalyzed by amylases, is the most important commercial enzyme process. The hydrolyzed products are widely applied in food, paper and textile industries. Because of the increasing demand for these enzymes in various industries, there is enormous interest in developing enzymes with better properties, such as raw starch-degrading amylases suitable for industrial applications and their cost-effective production techniques. Sorghum (Sorghum bicolor) is a widely grown crop in Africa. Obtaining enzymes from sorghum requires a transformation. The objective of this study was application of sorghum amylase for maltodextrin. Sorghum seeds were supplied by the ISRA (Bambey, Senegal). Seeds were germinated in the laboratory at 30°C for 72 h and the sorghum malt was dried at 40°C for 48 h. Corn starch (from Roquette, France) hydrolysis was assayed in a bioreactor of 2 l at a temperature of 65°C gently stirred. Raw starch was slurried in water (30% w/v) and sorghum malt was introduced, chloride calcium was added, pH was adjusted to 6. Maltodextrin was characterized in term of the dextrose equivalent (DE) during the hydrolysis. The yield of hydrolysis was evaluated by soluble solids (° BRIX) at different hydrolysis time. The glucose concentration released was measured by DNS method (Miller method). [less ▲]

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See detailA highly stable Yarrowia lipolytica lipase formulation for the treatment of pancreatic exocrine insufficiency
Turki, S.; Mrabet, G.; Jabloun, Z. et al

in Biotechnology & Applied Biochemistry (2010), 57(4), 139-149

Yarrowia lipolytica lipase has been assumed to be a good candidate for the treatment of fat malabsorption in patients with pancreatic insufficiency. Nevertheless, no systematic studies on its stability ... [more ▼]

Yarrowia lipolytica lipase has been assumed to be a good candidate for the treatment of fat malabsorption in patients with pancreatic insufficiency. Nevertheless, no systematic studies on its stability under physiological conditions pertaining to the human GI (gastrointestinal) tract have been published. Stability of various Y. lipolytica lipase powder formulations at various physiological pH values as well as the effect of digestive proteases and bile salts on enzyme activity were investigated. Results were compared with those obtained from another competing fungal lipase sourced fromCandida rugosa. Among the studied formulations, Y. lipolytica lipase stabilized with gum arabic and skimmed milk powder was the most promising powder formulation. Under acidic conditions (pH 3-5), this formulation showed higher stability than those observed with the other Y. lipolytica lipase formulations and C. rugosa lipase. In addition, in the presence of gum arabic and skimmed milk powder as additives, Y. lipolytica lipase exhibited markedly higher resistance to pepsin, trypsin and chymotrypsin actions. Resistance to proteolytic degradation by digestive proteases was also by far higher than that observed with C. rugosa lipase. Similar behaviour was, however, observed when these two fungal lipases were incubated with increased concentrations of bile salts. Residual lipase activity of both fungal lipases showed a slight decrease in NaTDC (sodium taurodeoxycholate) concentration above 4 mM. Consequently, Y. lipolytica lipase formulated with gum arabic and milk powder seemed to have great potential for use as a therapeutic tool for patients with pancreatic insufficiency. © 2010 Portland Press Limited. [less ▲]

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See detailThe utilization of gum tragacanth to improve the growth of Rhodotorula aurantiaca and the production of γ-decalactone in large scale
Alchihab, Mohamed ULg; Destain, Jacqueline ULg; Aguedo, Mario ULg et al

in Applied Biochemistry and Biotechnology (2010), 162(1), 233-241

The production of γ-decalactone and 4-hydroxydecanoic acid by the psychrophilic yeast R. aurantiaca was studied. The effect of both compounds on the growth of R. aurantiaca was also investigated and our ... [more ▼]

The production of γ-decalactone and 4-hydroxydecanoic acid by the psychrophilic yeast R. aurantiaca was studied. The effect of both compounds on the growth of R. aurantiaca was also investigated and our results show that γ-decalactone must be one of the limiting factors for its production. The addition of gum tragacanth to the medium at concentrations of 3 and 4 g/l seems to be an adequate strategy to enhance γ-decalactone production and to reduce its toxicity towards the cell. The production of γ-decalactone and 4- hydroxydecanoic acid was significantly higher in 20-l bioreactor than in 100-l bioreactor. By using 20 g/l of castor oil, 6.5 and 4.5 g/l of γ-decalactone were extracted after acidification at pH 2.0 and distillation at 100 °C for 45 min in 20- and 100-l bioreactors, respectively. We propose a process at industrial scale using a psychrophilic yeast to produce naturally γ-decalactone from castor oil which acts also as a detoxifying agent; moreover the process was improved by adding a natural gum. [less ▲]

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See detailProduction d’arômes de type lactone par des levures.
Alchihab, Mohamed ULg; Destain, Jacqueline ULg; Aguedo, Mario ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2010), 14(4), 681-691

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See detailLes produits de la mer au Sénégal et le potentiel des bactéries lactiques et des bactériocines pour la conservation
Diop, M.; Destain, Jacqueline ULg; Tine, E. et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2010), 14(2), 341-350

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See detailEtude comparative des composés phénoliques, du pouvoir antioxydant de différentes variétés de sorgho sénégalais et des enzymes amylolytiques de leur malt
Ba, Khady ULg; Tine, Emmanuel; Destain, Jacqueline ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2010), 14(1), 131-139

The study involved seven cultivars of white sorghum selected to ISRA Bambey and consumed in many regions of Senegal. Several characters and biochemical compounds of the grains (presence of pigmented testa ... [more ▼]

The study involved seven cultivars of white sorghum selected to ISRA Bambey and consumed in many regions of Senegal. Several characters and biochemical compounds of the grains (presence of pigmented testa, total phenols, condensed tannins and antioxidant activity) were determined in all cultivars. A three days malting was also carried out and amylolytic enzymes such as α-amylase, β-amylase and limit-dextrinase which are essential for a malt of good quality were proportioned. These enzymes are measured specifically with kits of Megazyme: Amylazyme (α-amylase), Betamyl (β-amylase) and Limit-Dextrizyme (limit-dextrinase). Two cultivars, CE 180-33 and CE 145-66 proved to be tannin sorghums, have the highest levels in total phenols and the most important antioxidant activities (ABTS and DPPH). The results of enzymatic analysis and the index of Kolbach indicated the F-2-20 like cultivar presenting the best potentialities for malt production [less ▲]

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See detailPeroxisomal β-oxidation and Production of γ-decalactone by the Yeast Rhodotorula aurantiaca
Alchihab, Mohamed ULg; Dubois Dauphin, Robin ULg; Aguedo, Mario ULg et al

in Notulae Botanicae Horti Agrobotanici Cluj-Napoca (2010), 2

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See detailThe use of Macronet resins to recover decalactone produced by Rhodotorula aurantiaca from the culture broth
Alchihab, Mohamed ULg; Aldric, Jean-Marc ULg; Aguedo, Mario ULg et al

in Journal of Industrial Microbiology & Biotechnology (2010), 37(2), 167-172

During the biotransformation of castor oil into -decalactone, R. aurantiaca produced both the lactone form and its precursor (4-hydroxydecanoic acid). After six days of culture, a maximum yield of ... [more ▼]

During the biotransformation of castor oil into -decalactone, R. aurantiaca produced both the lactone form and its precursor (4-hydroxydecanoic acid). After six days of culture, a maximum yield of -decalactone of 6.5 g/l was obtained. The parameters of -decalactone adsorption on three Macronet resins (MN-202, MN-102 and MN-100) were investigated in water. Adsorption isotherms of -decalactone for the three Macronet resins were linear. The trapping of -decalactone produced by R. aurantiaca on these resins was then carried out. -Decalactone was eVectively retained by all the studied Macronet resins. The resin MN-202 trapped -decalactone more eYciently than MN-102 and MN-100. The percentages of -decalactone adsorbed on the resins MN-202, MN-102 and MN-100 were, respectively, 85, 75 and 81%, whereas around 70% of the adsorbed -decalactone was then desorbed. We propose an industrial process that uses Macronet resins to extract -decalactone from culture broth of R. aurantiaca. [less ▲]

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See detailL'extrapolation des bioréacteurs : un problème de génie des procédés ou de physiologie microbienne ?
Delvigne, Frank ULg; Destain, Jacqueline ULg; Brognaux, Alison ULg et al

in Chimie Nouvelle (2010), 104

Bioreactor scale-up often pose a serious issue during the industrial development of a bioprocess considering the numerous physical and biological phenomena occurring in the reacting volume. The basic ... [more ▼]

Bioreactor scale-up often pose a serious issue during the industrial development of a bioprocess considering the numerous physical and biological phenomena occurring in the reacting volume. The basic principles of scale-up coming from the traditional chemical and process engineering approaches will be first reviewed and will be then compared to a new one involving recent development at the level of microbial strain manipulation. This "physiological" approach of scale-up involves directly a biological component of the system (by comparison with the traditional approach for scaling-up involving physical parameters indirectly linked to the physiological phenomena occurring in the bioreactor), i.e. the synthesis of a reporter fluorescent protein when microbial cells are exposed to stress. It will be shown how this principle can be used for a better understanding of the relationship between bioreactor hydrodynamics and microbial stress. [less ▲]

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See detailImpact of scaled-down dissolved oxygen fluctuations at different levels of the lipase synthesis pathway of Yarrowia lipolytica
Kar, Tambi ULg; Destain, Jacqueline ULg; Thonart, Philippe ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2010), 14

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See detailHydrolysis of starch by sorghum malt for maltodextrin production
Ba, Khady ULg; Destain, Jacqueline ULg; Thonart, Philippe ULg

Poster (2009, December 16)

Maltodextrin is a mixture of saccharides with a molecular weight between polysaccharides and oligosaccharides with DE lower than 20. Maltodextrin is more soluble in water than native starches, also is ... [more ▼]

Maltodextrin is a mixture of saccharides with a molecular weight between polysaccharides and oligosaccharides with DE lower than 20. Maltodextrin is more soluble in water than native starches, also is cheaper in comparison with other major edible hydrocolloids. Maltodextrin is obtained by moderate enzymatic or acidic hydrolysis of starch. The hydrolysis of starch, catalyzed by amylases, is the most important commercial enzyme process. The hydrolyzed products are widely applied in food, paper and textile industries. Because of the increasing demand for these enzymes in various industries, there is enormous interest in developing enzymes with better properties, such as raw starch-degrading amylases suitable for industrial applications and their cost-effective production techniques. Sorghum (Sorghum bicolor) is a widely grown crop in Africa. Obtaining enzymes from sorghum requires a transformation. The objective of this study was application of sorghum amylase for maltodextrin. Sorghum seeds were supplied by the ISRA (Bambey, Senegal). Seeds were germinated in the laboratory at 30°C for 72 h and the sorghum malt was dried at 40°C for 48 h. Corn starch (from Roquette, France) hydrolysis was assayed in a bioreactor of 2 l at a temperature of 65°C gently stirred. Raw starch was slurried in water (30% w/v) and sorghum malt was introduced, chloride calcium was added, pH was adjusted to 6. Maltodextrin was characterized in term of the dextrose equivalent (DE) during the hydrolysis. The yield of hydrolysis was evaluated by soluble solids (° BRIX) at different hydrolysis time. The glucose concentration released was measured by DNS method (Miller method). [less ▲]

Detailed reference viewed: 126 (4 ULg)