References of "Destain, Jacqueline"
     in
Bookmark and Share    
Full Text
See detailLa production de bioéthanol à partir de biomasse lignocellulosique
Didderen, I.; Destain, Jacqueline ULg; Thonart, Philippe ULg

Article for general public (2010)

Detailed reference viewed: 47 (6 ULg)
Full Text
Peer Reviewed
See detailIsolation, pure culture and characterization of Serratia symbiotica, the R-type of secondary endosymbionts of the black bean aphid Aphis fabae.
Sabri, Ahmed ULg; Leroy, Pascal ULg; Haubruge, Eric ULg et al

in International Journal of Systematic and Evolutionary Microbiology (2010)

An intracellular symbiotic bacterium was isolated from the flora of a natural clone of the black bean aphid Aphis fabae. The strain was able to grow freely in aerobic condition on a rich medium containing ... [more ▼]

An intracellular symbiotic bacterium was isolated from the flora of a natural clone of the black bean aphid Aphis fabae. The strain was able to grow freely in aerobic condition on a rich medium containing 1% of each of following substrates; dextrose, yeast extract and peptone of casein. Pure culture was achieved through the use of solid-phase culture on the same medium and was designated CWBI-2.3T. 16S ribosomal DNA sequence analysis revealed that CWBI-2.3T strain was a member of the gamma-3 subgroup of the class Proteobacteria, having high sequence identity (>99%) with the R-Type of secondary endosymbiont found in several aphid species Serratia symbiotica. As strain CWBI-2.3T (=LMG 25624T =DSM 23270T) was the first R-type symbiont to be isolated and characterized, we proposed to designate it as the type strain of Serratia symbiotica species. [less ▲]

Detailed reference viewed: 168 (40 ULg)
Full Text
See detailHydrolysis of starch by sorghum malt for maltodextrin production
Ba, K.; Destain, Jacqueline ULg; Thonart, Philippe ULg

Scientific conference (2010)

Maltodextrin is a mixture of saccharides with a molecular weight between polysaccharides and oligosaccharides with DE lower than 20. Maltodextrin is more soluble in water than native starches, also is ... [more ▼]

Maltodextrin is a mixture of saccharides with a molecular weight between polysaccharides and oligosaccharides with DE lower than 20. Maltodextrin is more soluble in water than native starches, also is cheaper in comparison with other major edible hydrocolloids. Maltodextrin is obtained by moderate enzymatic or acidic hydrolysis of starch. The hydrolysis of starch, catalyzed by amylases, is the most important commercial enzyme process. The hydrolyzed products are widely applied in food, paper and textile industries. Because of the increasing demand for these enzymes in various industries, there is enormous interest in developing enzymes with better properties, such as raw starch-degrading amylases suitable for industrial applications and their cost-effective production techniques. Sorghum (Sorghum bicolor) is a widely grown crop in Africa. Obtaining enzymes from sorghum requires a transformation. The objective of this study was application of sorghum amylase for maltodextrin. Sorghum seeds were supplied by the ISRA (Bambey, Senegal). Seeds were germinated in the laboratory at 30°C for 72 h and the sorghum malt was dried at 40°C for 48 h. Corn starch (from Roquette, France) hydrolysis was assayed in a bioreactor of 2 l at a temperature of 65°C gently stirred. Raw starch was slurried in water (30% w/v) and sorghum malt was introduced, chloride calcium was added, pH was adjusted to 6. Maltodextrin was characterized in term of the dextrose equivalent (DE) during the hydrolysis. The yield of hydrolysis was evaluated by soluble solids (° BRIX) at different hydrolysis time. The glucose concentration released was measured by DNS method (Miller method). [less ▲]

Detailed reference viewed: 13 (1 ULg)
Full Text
See detailDetermination of phospholipids extracted from Tsukamurella paurometabola C-924 after freeze-drying and during the subsequent storage
Coulibaly, I.; Dauphin, R. D.; Destain, Jacqueline ULg et al

Scientific conference (2010)

The total lipids of Tsukamurella paurometabola C-924 were analyzed after freeze-drying. Seven individual lipids classes were identifiednamelyneutrallipids(NLs),fattyacids(FAs),phospholipids(PLs ... [more ▼]

The total lipids of Tsukamurella paurometabola C-924 were analyzed after freeze-drying. Seven individual lipids classes were identifiednamelyneutrallipids(NLs),fattyacids(FAs),phospholipids(PLs),sterolester(SEs),triglycerides(TGs),diglycerides (DGs) and monoglycerides (MGs). The principal fatty acids identified in most lipid classes were palmitic (C 16:0), palmitoleic (C 16:1), oleic (C 18:1), linoleic (C 18:2), and linolenic (C 18:3). PLs were the major constituents and accounted for 50-60% of the total lipids. PLs were fractionated. PLs of Tsukamurella paurometabola content phosphatidic acid (PA), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylcholine (PC), sphingomyelin (SM), lysophosphatidylcholine (LPC) and phosphatidylglycerol (PG). It was observed that PG had the highest proportion at most points relative to other PLs and was the predominant component of PLs (30%-56%). Evolution of individual rate was followed during storage at 20°C and 40°C with or without lithothamne400®, respectively. [less ▲]

Detailed reference viewed: 12 (2 ULg)
Full Text
Peer Reviewed
See detailA highly stable Yarrowia lipolytica lipase formulation for the treatment of pancreatic exocrine insufficiency
Turki, S.; Mrabet, G.; Jabloun, Z. et al

in Biotechnology & Applied Biochemistry (2010), 57(4), 139-149

Yarrowia lipolytica lipase has been assumed to be a good candidate for the treatment of fat malabsorption in patients with pancreatic insufficiency. Nevertheless, no systematic studies on its stability ... [more ▼]

Yarrowia lipolytica lipase has been assumed to be a good candidate for the treatment of fat malabsorption in patients with pancreatic insufficiency. Nevertheless, no systematic studies on its stability under physiological conditions pertaining to the human GI (gastrointestinal) tract have been published. Stability of various Y. lipolytica lipase powder formulations at various physiological pH values as well as the effect of digestive proteases and bile salts on enzyme activity were investigated. Results were compared with those obtained from another competing fungal lipase sourced fromCandida rugosa. Among the studied formulations, Y. lipolytica lipase stabilized with gum arabic and skimmed milk powder was the most promising powder formulation. Under acidic conditions (pH 3-5), this formulation showed higher stability than those observed with the other Y. lipolytica lipase formulations and C. rugosa lipase. In addition, in the presence of gum arabic and skimmed milk powder as additives, Y. lipolytica lipase exhibited markedly higher resistance to pepsin, trypsin and chymotrypsin actions. Resistance to proteolytic degradation by digestive proteases was also by far higher than that observed with C. rugosa lipase. Similar behaviour was, however, observed when these two fungal lipases were incubated with increased concentrations of bile salts. Residual lipase activity of both fungal lipases showed a slight decrease in NaTDC (sodium taurodeoxycholate) concentration above 4 mM. Consequently, Y. lipolytica lipase formulated with gum arabic and milk powder seemed to have great potential for use as a therapeutic tool for patients with pancreatic insufficiency. © 2010 Portland Press Limited. [less ▲]

Detailed reference viewed: 7 (0 ULg)
Full Text
Peer Reviewed
See detailThe utilization of gum tragacanth to improve the growth of Rhodotorula aurantiaca and the production of γ-decalactone in large scale
Alchihab, Mohamed ULg; Destain, Jacqueline ULg; Aguedo, Mario ULg et al

in Applied Biochemistry and Biotechnology (2010), 162(1), 233-241

The production of γ-decalactone and 4-hydroxydecanoic acid by the psychrophilic yeast R. aurantiaca was studied. The effect of both compounds on the growth of R. aurantiaca was also investigated and our ... [more ▼]

The production of γ-decalactone and 4-hydroxydecanoic acid by the psychrophilic yeast R. aurantiaca was studied. The effect of both compounds on the growth of R. aurantiaca was also investigated and our results show that γ-decalactone must be one of the limiting factors for its production. The addition of gum tragacanth to the medium at concentrations of 3 and 4 g/l seems to be an adequate strategy to enhance γ-decalactone production and to reduce its toxicity towards the cell. The production of γ-decalactone and 4- hydroxydecanoic acid was significantly higher in 20-l bioreactor than in 100-l bioreactor. By using 20 g/l of castor oil, 6.5 and 4.5 g/l of γ-decalactone were extracted after acidification at pH 2.0 and distillation at 100 °C for 45 min in 20- and 100-l bioreactors, respectively. We propose a process at industrial scale using a psychrophilic yeast to produce naturally γ-decalactone from castor oil which acts also as a detoxifying agent; moreover the process was improved by adding a natural gum. [less ▲]

Detailed reference viewed: 82 (33 ULg)
Full Text
Peer Reviewed
See detailProduction d’arômes de type lactone par des levures.
Alchihab, Mohamed ULg; Destain, Jacqueline ULg; Aguedo, Mario ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2010), 14(4), 681-691

Detailed reference viewed: 18 (6 ULg)
Full Text
Peer Reviewed
See detailLes produits de la mer au Sénégal et le potentiel des bactéries lactiques et des bactériocines pour la conservation
Diop, M.; Destain, Jacqueline ULg; Tine, E. et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2010), 14(2), 341-350

Detailed reference viewed: 11 (3 ULg)
Full Text
Peer Reviewed
See detailEtude comparative des composés phénoliques, du pouvoir antioxydant de différentes variétés de sorgho sénégalais et des enzymes amylolytiques de leur malt
Ba, Khady ULg; Tine, Emmanuel; Destain, Jacqueline ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2010), 14(1), 131-139

The study involved seven cultivars of white sorghum selected to ISRA Bambey and consumed in many regions of Senegal. Several characters and biochemical compounds of the grains (presence of pigmented testa ... [more ▼]

The study involved seven cultivars of white sorghum selected to ISRA Bambey and consumed in many regions of Senegal. Several characters and biochemical compounds of the grains (presence of pigmented testa, total phenols, condensed tannins and antioxidant activity) were determined in all cultivars. A three days malting was also carried out and amylolytic enzymes such as α-amylase, β-amylase and limit-dextrinase which are essential for a malt of good quality were proportioned. These enzymes are measured specifically with kits of Megazyme: Amylazyme (α-amylase), Betamyl (β-amylase) and Limit-Dextrizyme (limit-dextrinase). Two cultivars, CE 180-33 and CE 145-66 proved to be tannin sorghums, have the highest levels in total phenols and the most important antioxidant activities (ABTS and DPPH). The results of enzymatic analysis and the index of Kolbach indicated the F-2-20 like cultivar presenting the best potentialities for malt production [less ▲]

Detailed reference viewed: 473 (12 ULg)
Full Text
Peer Reviewed
See detailPeroxisomal β-oxidation and Production of γ-decalactone by the Yeast Rhodotorula aurantiaca
Alchihab, Mohamed ULg; Dubois Dauphin, Robin ULg; Aguedo, Mario ULg et al

in Notulae Botanicae Horti Agrobotanici Cluj-Napoca (2010), 2

Detailed reference viewed: 29 (13 ULg)
Full Text
Peer Reviewed
See detailThe use of Macronet resins to recover decalactone produced by Rhodotorula aurantiaca from the culture broth
Alchihab, Mohamed ULg; Aldric, Jean-Marc ULg; Aguedo, Mario ULg et al

in Journal of Industrial Microbiology & Biotechnology (2010), 37(2), 167-172

During the biotransformation of castor oil into -decalactone, R. aurantiaca produced both the lactone form and its precursor (4-hydroxydecanoic acid). After six days of culture, a maximum yield of ... [more ▼]

During the biotransformation of castor oil into -decalactone, R. aurantiaca produced both the lactone form and its precursor (4-hydroxydecanoic acid). After six days of culture, a maximum yield of -decalactone of 6.5 g/l was obtained. The parameters of -decalactone adsorption on three Macronet resins (MN-202, MN-102 and MN-100) were investigated in water. Adsorption isotherms of -decalactone for the three Macronet resins were linear. The trapping of -decalactone produced by R. aurantiaca on these resins was then carried out. -Decalactone was eVectively retained by all the studied Macronet resins. The resin MN-202 trapped -decalactone more eYciently than MN-102 and MN-100. The percentages of -decalactone adsorbed on the resins MN-202, MN-102 and MN-100 were, respectively, 85, 75 and 81%, whereas around 70% of the adsorbed -decalactone was then desorbed. We propose an industrial process that uses Macronet resins to extract -decalactone from culture broth of R. aurantiaca. [less ▲]

Detailed reference viewed: 130 (31 ULg)
Full Text
See detailL'extrapolation des bioréacteurs : un problème de génie des procédés ou de physiologie microbienne ?
Delvigne, Frank ULg; Destain, Jacqueline ULg; Brognaux, Alison ULg et al

in Chimie Nouvelle (2010), 104

Bioreactor scale-up often pose a serious issue during the industrial development of a bioprocess considering the numerous physical and biological phenomena occurring in the reacting volume. The basic ... [more ▼]

Bioreactor scale-up often pose a serious issue during the industrial development of a bioprocess considering the numerous physical and biological phenomena occurring in the reacting volume. The basic principles of scale-up coming from the traditional chemical and process engineering approaches will be first reviewed and will be then compared to a new one involving recent development at the level of microbial strain manipulation. This "physiological" approach of scale-up involves directly a biological component of the system (by comparison with the traditional approach for scaling-up involving physical parameters indirectly linked to the physiological phenomena occurring in the bioreactor), i.e. the synthesis of a reporter fluorescent protein when microbial cells are exposed to stress. It will be shown how this principle can be used for a better understanding of the relationship between bioreactor hydrodynamics and microbial stress. [less ▲]

Detailed reference viewed: 193 (52 ULg)
Full Text
Peer Reviewed
See detailImpact of scaled-down dissolved oxygen fluctuations at different levels of the lipase synthesis pathway of Yarrowia lipolytica
Kar, Tambi ULg; Destain, Jacqueline ULg; Thonart, Philippe ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2010), 14

Detailed reference viewed: 35 (14 ULg)
Full Text
Peer Reviewed
See detailHydrolysis of starch by sorghum malt for maltodextrin production
Ba, Khady ULg; Destain, Jacqueline ULg; Thonart, Philippe ULg

Poster (2009, December 16)

Maltodextrin is a mixture of saccharides with a molecular weight between polysaccharides and oligosaccharides with DE lower than 20. Maltodextrin is more soluble in water than native starches, also is ... [more ▼]

Maltodextrin is a mixture of saccharides with a molecular weight between polysaccharides and oligosaccharides with DE lower than 20. Maltodextrin is more soluble in water than native starches, also is cheaper in comparison with other major edible hydrocolloids. Maltodextrin is obtained by moderate enzymatic or acidic hydrolysis of starch. The hydrolysis of starch, catalyzed by amylases, is the most important commercial enzyme process. The hydrolyzed products are widely applied in food, paper and textile industries. Because of the increasing demand for these enzymes in various industries, there is enormous interest in developing enzymes with better properties, such as raw starch-degrading amylases suitable for industrial applications and their cost-effective production techniques. Sorghum (Sorghum bicolor) is a widely grown crop in Africa. Obtaining enzymes from sorghum requires a transformation. The objective of this study was application of sorghum amylase for maltodextrin. Sorghum seeds were supplied by the ISRA (Bambey, Senegal). Seeds were germinated in the laboratory at 30°C for 72 h and the sorghum malt was dried at 40°C for 48 h. Corn starch (from Roquette, France) hydrolysis was assayed in a bioreactor of 2 l at a temperature of 65°C gently stirred. Raw starch was slurried in water (30% w/v) and sorghum malt was introduced, chloride calcium was added, pH was adjusted to 6. Maltodextrin was characterized in term of the dextrose equivalent (DE) during the hydrolysis. The yield of hydrolysis was evaluated by soluble solids (° BRIX) at different hydrolysis time. The glucose concentration released was measured by DNS method (Miller method). [less ▲]

Detailed reference viewed: 116 (4 ULg)
Full Text
See detailLe développement de starters lactiques : de la cellule au produit fini
Thonart, Philippe ULg; Kouakou, P.; Dortu, C. et al

Conference (2009, November 02)

Detailed reference viewed: 49 (14 ULg)
Full Text
See detailRecovery of γ-decalactone produced by Rhodotorula aurantiaca from the culture broth using Macronet resins
Alchihab, Mohamed; Aldric, Jean-Marc ULg; Aguedo, Mario ULg et al

in New Biotechnology (2009, September), 25S

Detailed reference viewed: 8 (2 ULg)
Full Text
Peer Reviewed
See detailProduction of γ-Decalactone by a Psychrophilic and a Mesophilic Strain of the Yeast
Alchihab, Mohamed ULg; Destain, Jacqueline ULg; Aguedo, Mario ULg et al

in Applied Biochemistry and Biotechnology (2009), 158

Among 18 psychrophilic strains isolated near the Antarctic Station, the psychrophilic <br />strain Rhodotorula aurantiaca A19 was selected for its ability of growth and γ- <br />decalactone production at ... [more ▼]

Among 18 psychrophilic strains isolated near the Antarctic Station, the psychrophilic <br />strain Rhodotorula aurantiaca A19 was selected for its ability of growth and γ- <br />decalactone production at low temperatures. The effects of temperature, initial pH, and castor <br />oil concentration on the growth and γ-decalactone production by a psychrophilic and a <br />mesophilic strain of R. aurantiaca were investigated. The highest γ-decalactone production <br />in flasks (5.8 g/l) was obtained with the strain A19 at 14 °C and initial pH 7.0 in medium <br />containing 20 g/l castor oil. On the other hand, these factors did not affect the production of <br />γ-decalactone by the mesophilic strain. In fermentor, a γ-decalactone concentration of 6.6 g/l <br />was reached with the strain A19, whereas a maximum of 0.1 g/l was obtained with the <br />mesophilic strain. Our results suggest that the ability to synthesize γ-decalactone is a <br />particularity of the strain A19, since the mesophilic strain (no. 30645) produced small amounts, <br />and the other (no. 31354) did not exhibit this property. It is, to our knowledge, the first report of γ-decalactone production by R. aurantiaca and furthermore by a psychrophilic yeast strain. <br />Moreover, the amount of γ-decalactone obtained in fermentor with the strain 19 was on the <br />order of concentrations usually described in patents. [less ▲]

Detailed reference viewed: 113 (41 ULg)
Full Text
See detailThe use of Macronet resins to recover γ-decalactone produced by Rhodotorula aurantiaca from the culture broth
Alchihab, Mohamed ULg; Destain, Jacqueline ULg; Aguedo, Mario ULg et al

Poster (2009)

The objective of this study was to recover the γ-decalactone produced by R. aurantiaca from the culture broth using three Macronet resins (MN-100, MN-102 and MN-202).

Detailed reference viewed: 27 (4 ULg)