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See detailUse of Bacillus subtilis S499 to control mould growth during malting of red sorghum from the D.R. of Congo
Bwanganga Tawaba, Jean-Claude ULg; Destain, Jacqueline ULg; Bera, François ULg et al

Poster (2011, November)

In this work, moulds growth was monitored during malting of a red sorghum from D.R. of Congo. Results obtained with chemical treatments [diluted alkalines: NaOH 0.2% (T1) and Ca(OH)2 0.1% (T2)] were ... [more ▼]

In this work, moulds growth was monitored during malting of a red sorghum from D.R. of Congo. Results obtained with chemical treatments [diluted alkalines: NaOH 0.2% (T1) and Ca(OH)2 0.1% (T2)] were compared with those of biocontrols [use of Bacillus subtilis S499 108 cells / mL: supernatant + cells (T3), cells (T4) and cells-free supernatant (T5)]. Chemical treatments have shown the highest fungal contamination levels, the highest malting loss, but better enzyme activities (α and β-amylases, limit-dextrinase, α-glucosidase and β-glucanase). The inhibition tests showed that the concentration of lipopeptides is correlated with the reduction of moulds growth. Malting trials with starters of Bacillus subtilis S499 showed that culture dilution affects the growth of moulds during red sorghum malting. The combination of a chemical treatment such as diluted NaOH with a biocontrol could be used to improve sorghum malts quality in terms of different enzymes activities and by reducing the level of fungal contamination. Key words: Bacillus subtilis S499, mould, sorghum malting [less ▲]

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See detailChanges in termites feeding diets for gut micro-organisms selection and further cultivation
Bauwens, Julien ULg; Brasseur, Catherine ULg; Matteotti, Christel ULg et al

Poster (2011, October 02)

Termites gut may overcome important dietary perturbations, initial diversity acting as key point buffering effects on host, although termites possess their own enzymatic system. Some artificial diets ... [more ▼]

Termites gut may overcome important dietary perturbations, initial diversity acting as key point buffering effects on host, although termites possess their own enzymatic system. Some artificial diets permitted a simplification of the lower termites gut symbiotic system, which could be used as first step in symbionts isolation and cultivation. Preliminary assay of cultivation actually gave encouraging results. Proteomic proved to be suitable tool to investigate such a complex system. Nevertheless, for some symbionts very few genes are sequenced, which should lead to more targeted proteomic studies. Protein chromatography will allow to split up the proteome and more accurate analysis. [less ▲]

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See detailPotentialités d’application des technologies biologiques pour la depollution des sols en Wallonie
Aldric, Jean-Marc ULg; Druart, P.; Maesen, Philippe ULg et al

in Journal des Ingénieurs (Le) (2011), 132

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See detailEx vivo ruminal cellulosome for by-product biomass conversion
Hissette, Mathias ULg; Destain, Jacqueline ULg; Thewis, André ULg et al

Conference (2011, April 27)

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See detailIn vitro culture of the parasitic wasp Aphidius ervi: Sweet dream or Reality
Sabri, Ahmed ULg; Leroy, Pascal ULg; Haubruge, Eric ULg et al

Poster (2011, March 03)

Aphidius ervi is an entomophagous parasitoid and represents an effective parasitoid of several aphid species that cause great damages in agriculture. Several investigations, for its in vitro mass ... [more ▼]

Aphidius ervi is an entomophagous parasitoid and represents an effective parasitoid of several aphid species that cause great damages in agriculture. Several investigations, for its in vitro mass production, have achieved a limited success and suggest that in vitro culture of this valuable biological control agent is rather closer to a dream than reality. Our work provides a chronological study of A. ervi development, from the oviposition until hatching of the first instar larva, in the body of the pea aphid Acyrthosiphon pisum. We show that this parasitoid has some characteristics in its embryological development that are rather complex and different from most other reported insects, which can be phylogenetically very close. Some of these characteristics concern extraembryonic membranes and could be among the causes of the limited success achieved in the in vitro culture. [less ▲]

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See detailBiochemistry of lactone formation in yeast and fungi and its utilisation for the production of flavour and fragrance compounds
Romero-Guido, C.; Belo, I.; Ta, T. M. N. et al

in Applied Microbiology & Biotechnology (2011), 89

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See detailDimensionnement et extrapolation des bioréacteurs sur base de paramètres physiologiques : cas de la production de lipase par Yarrowia lipolytica
Kar, Tambi ULg; Delvigne, Frank ULg; Destain, Jacqueline ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2011), 15(4), 585-595

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See detailHigh-level production of extracellular lipase by Yarrowia lipolytica mutants from methyl oleate
Darvishi, F.; Destain, Jacqueline ULg; Nahvi, I. et al

in New Biotechnology (2011), 28(6), 756-760

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See detailTechniques de séchage des starters lactiques et mécanismes affectant la viabilité cellulaire suite à la lyophilisation
Coulibaly, Ibourahema ULg; Dubois Dauphin, Robin ULg; Danthine, Sabine ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2011), 15(2), 287-299

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See detailPlacenta-Like Structure of the Aphid Endoparasitic Wasp Aphidius ervi: A Strategy of Optimal Resources Acquisition.
Sabri, Ahmed ULg; Hance, Thierry; Leroy, Pascal ULg et al

in PloS one (2011), 6(4), 18847

Aphidius ervi (Hymenoptera: Braconidae) is an entomophagous parasitoid known to be an effective parasitoid of several aphid species of economic importance. A reduction of its production cost during mass ... [more ▼]

Aphidius ervi (Hymenoptera: Braconidae) is an entomophagous parasitoid known to be an effective parasitoid of several aphid species of economic importance. A reduction of its production cost during mass rearing for inundative release is needed to improve its use in biological control of pests. In these contexts, a careful analysis of its entire development phases within its host is needed. This paper shows that this parasitoid has some characteristics in its embryological development rather complex and different from most other reported insects, which can be phylogenetically very close. First, its yolkless egg allows a high fecundity of the female but force them to hatch from the egg shell rapidly to the host hemocoel. An early cellularisation allowing a rapid differentiation of a serosa membrane seems to confirm this hypothesis. The serosa wraps the developing embryo until the first instar larva stage and invades the host tissues by microvilli projections and form a placenta like structure able to divert host resources and allowing nutrition and respiration of embryo. Such interspecific invasion, at the cellular level, recalls mammal's trophoblasts that anchors maternal uterine wall and underlines the high adaptation of A. ervi to develop in the host body. [less ▲]

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See detailLa biométhanisation : technologies de choix
Hiligsmann, Serge ULg; Hamilton, Christopher; Beckers, Laurent et al

Conference (2010, November 23)

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See detailCaractérisation de la diversité des organismes symbiotiques et des activités glycosyl hydrolases dans le tube digestif de Reticulitermes santonensis (Feytaud) par une approche multidisciplinaire
Bauwens, Julien ULg; Matteotti, Christel ULg; Brognaux, Alison ULg et al

Scientific conference (2010, July 08)

Le bioéthanol cellulosique pourrait être une solution pour satisfaire le besoin croissant en énergie renouvelable. Actuellement, l’efficience de la transformation de la cellulose en sucres fermentescibles ... [more ▼]

Le bioéthanol cellulosique pourrait être une solution pour satisfaire le besoin croissant en énergie renouvelable. Actuellement, l’efficience de la transformation de la cellulose en sucres fermentescibles reste le principal facteur limitant. La recherche de nouvelles glycosyl hydrolases constitue une voie potentielle d’amélioration de la valorisation des composés ligno-cellulosiques. Trois types de glycosyl hydrolases sont généralement produites par les organismes capables d’utiliser efficacement ces composés : les endoglucanases, les exoglucanases/cellobiohydrolases, et les β-glucosidases. Dans les processus de digestion de la cellulose par les animaux, des organismes symbiotiques tels que des bactéries, des protistes et/ou des champignons sont fréquemment observés. Ces organismes contribuent en grande partie voir totalement à la production des complexes enzymatiques nécessaires. Chez les termites inférieures, comme notre modèle Reticulitermes santonensis (Feytaud), des protistes et des bactéries sont impliqués dans un système symbiotique complexe. Une étude multidisciplinaire est menée afin d’approfondir les rôles respectifs des différents groupes de symbiontes, via des approches « omiques », à savoir la protéomique (ESI-LC-MS-MS, 2D-SDS-PAGE couplée avec une analyse en spectrométrie de masse du type MALDI-TOF pour l’identification des protéines), la génomique (avec une approche métagénomique basée sur la construction d’une large banque de cDNA), la métabolomique (caractérisation des produits de dégradation de carbohydrates via une strategie LC-MS). De plus, l’isolation de microorganismes a également été employée dans la caractérisation de la diversité et de l’activité des glycosyl hydrolases chez R. santonensis. [less ▲]

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