References of "Destain, Jacqueline"
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See detailEffect of humic substances on in vitro rooting of alder (Alnus glutinosa (L.) Gaertn) and birch (Betula pendula Roth)
Tahiri, Abdelghani ULg; Destain, Jacqueline ULg; Druart, Philippe et al

Poster (2013)

Humic substances (HS) are organic compounds resulting from the physical, chemical and microbiological transformations of organic residues. Present everywhere in the nature; they are taking part in basic ... [more ▼]

Humic substances (HS) are organic compounds resulting from the physical, chemical and microbiological transformations of organic residues. Present everywhere in the nature; they are taking part in basic functionalities in any ecosystems involving soils, sediments, water and landfills. They are heterogeneous and complex carbon macromolecules. Our study aims to compare the effect of HS from différent sources on in vitro rooting of woody plants. [less ▲]

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See detailPhysicochemical characterization of dextrins prepared with amylases from sorghum malt
Ba, Khady; Blecker, Christophe ULg; Danthine, Sabine ULg et al

in Stärke = Starch (2013)

Dextrins are partially hydrolyzed starch products which are used in a wide range of applications. Hydrolysis can be performed by acid or enzymatic reaction. However the properties of dextrins are ... [more ▼]

Dextrins are partially hydrolyzed starch products which are used in a wide range of applications. Hydrolysis can be performed by acid or enzymatic reaction. However the properties of dextrins are influenced by the type of reaction and the source of starch. The aim of the present study was to determine physicochemical properties of dextrins obtained from hydrolysis of starches (corn and wheat) and flours (wheat and cassava) by using sorghum malt amylases. Hydrolysis of starches and flours was performed at 65°C, the hydrolysates were centrifuged and spray-dried for analysis. Physicochemical and structure of the powders were investigated. Results showed that the proportion of DP2 (maltose and isomaltose) was higher in dextrins and more in dextrins from wheat flour. Low water activity and low dissolution time were found in all dextrins. XRD patterns revealed that the crystalline structure partially disappeared in some of them. At 30% concentration, we noted a Newtonian behavior for the dextrins. The freeze-concentrated T0 g and the peak melting temperature (T0 m) determined by DSC (DSC) showed lowest values for dextrins from wheat. Thermogravimetric analysis (TGA) revealed that the dextrins were quite stable until 200°C. [less ▲]

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See detailMunkoyo : des racines comme sources potentielles en enzymes amylolytiques et une boisson fermentée traditionnelle (synthèse bibliographique)
Foma, Roland Kibwega; Destain, Jacqueline ULg; Kayisu, Kalenga et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2013), 17(2), 352-363

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See detailInfluence of lignin in Reticulitermes santonensis: symbiotic interations investigated through proteomics
Bauwens, Julien ULg; Tarayre, Cédric ULg; Brasseur, Catherine ULg et al

in Symbiosis (2013)

The gut of lower termites is populated by numerous microbial species belonging to prokaryotes, fungi, yeasts and protists. These micro-organisms are organized in a complex symbiotic system, interacting ... [more ▼]

The gut of lower termites is populated by numerous microbial species belonging to prokaryotes, fungi, yeasts and protists. These micro-organisms are organized in a complex symbiotic system, interacting together and with the insect host. Their likely ability to degrade ligno-cellulosic compounds could lead to improvements in second generation biofuels production. Lignin elimination represents a critical point as this polymer significantly interferes with industrial process of cellulose. Although host produces its own lignin-degrading enzymes, some symbionts may participate in digestion of lignin and its degradation products in termite gut. Here, we compared gut proteomes from R. santonensis after rearing on artificial diets composed of cellulose with and without lignin. The effect of lignin in artificial diets on different parts of the digestive tract was compared through liquid chromatography associated with tandem mass spectrometry (LC-MS/MS) experiments. Enzymatic assays were performed to characterize activities present in R. santonensis digestive tract after feeding on artificial diets. Microscopic observations of microbial communities provided some information on population balances after feeding experiment. [less ▲]

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See detailHydrolysis of starches and flours by sorghum malt amylases for dextrins production
Ba, Khady ULg; Aguedo, Mario ULg; Tine, Emmanuel et al

in European Food Research & Technology (2013)

Corn and wheat starches as well as wheat and cassava flours were hydrolyzed using sorghum malt at 65 °C for 6 hours. During these reactions, dextrose equivalent (DE) values were followed under 3 ... [more ▼]

Corn and wheat starches as well as wheat and cassava flours were hydrolyzed using sorghum malt at 65 °C for 6 hours. During these reactions, dextrose equivalent (DE) values were followed under 3 concentrations of sorghum malt and of calcium chloride. Wheat flour presented the highest DE values and cassava flour had the highest hydrolysis yield. Thus, different dextrins were produced in a pilot plant and were analyzed by HPSEC and HPAEC-PAD for their molecular weight distribution and oligosaccharides composition respectively. The results indicated that oligosaccharides with broad molecular weight distributions were present in the dextrins produced and that the proportion of maltose was very high. [less ▲]

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See detailUtilisation des termites comme source de microorganismes dans la filière de production du bioéthanol de seconde génération
Tarayre, Cédric ULg; Bauwens, Julien ULg; Brasseur, Catherine ULg et al

Poster (2012, November 14)

Les termites abritent une microflore symbiotique qui intervient dans la dégradation des fibres constitutives du bois, synthétisant des enzymes capables d’hydrolyser ses composants. Les sucres ... [more ▼]

Les termites abritent une microflore symbiotique qui intervient dans la dégradation des fibres constitutives du bois, synthétisant des enzymes capables d’hydrolyser ses composants. Les sucres fermentescibles libérés suite à cette hydrolyse sont utilisables dans le cadre de la production du bioéthanol de seconde génération. [less ▲]

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See detailEffects of glycerol on Pseudomonas fluorescens BTP1 freeze-dried
Mputu Kanyinda, Jean-Noël ULg; Pierart, C.; Weekers, F. et al

in International Journal of Biotechnology and Biochemistry. (2012), 8(2), 245-258

The storage stability of freeze-dried powders was studied by parameters such as loss of viability on the Plate Count Agar (PCA). Powder with glycerol (PG) contains 8.4x1010cfu/g before storage 1 ... [more ▼]

The storage stability of freeze-dried powders was studied by parameters such as loss of viability on the Plate Count Agar (PCA). Powder with glycerol (PG) contains 8.4x1010cfu/g before storage 1.1x1010cfug after 3 months at 4°C and 6.0x108cfu/g after 3 months at 20°C. The concentration of soluble proteins (mg/g) decrease during storage at 4°C from 3.77 to 0.80 after 90 days; and the ratios of unsaturated to saturated fatty acids (C18:3/C16:0 and C18:2/C16:0) decrease respectively from 0.05 to 0.04 and 0.007 to 0.004 after 3 months at 4°C. This ratio characterises the membrane fluidity. Powder without glycerol (PS) contains 1.1x1010 cfu/g before storage and 1.4 x 108 cfu/g after 3 months at 4°C and 1.4 x 107 cfu/g after 3 months at 20°C. The concentration of soluble proteins (mg/g) decrease during storage at 4°C from 4.08 to 0.42 after 90 days, the glutathione concentration decrease during storage at 4°C from 2.2 to 1.4. The beneficial effect of glycerol on fatty acid composition during freezedrying is shown and the ratios of unsaturated to saturated fatty acids (C18:2/C16:0 and C18:3/C16:0) decrease respectively from 0.019 to 0.004 and 0.054 to 0.036 after 90 days storage at 4°C. Analysis by flow cytometry was used to assess the physiological state in which cells are at the end of freeze-drying. We found 13.5% live cells, 36.1% dead cells and 50.4% cells in an intermediate state for powder with glycerol (PG) after freeze-drying. These results shows that glycerol play an important role in Pseudomonas fluorescens BTP1 desiccation during freeze-drying, by maintaining a degree of viability after freeze-drying and during storage. [less ▲]

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See detailTermites artificially-fed on unusual diet and resulting enzymatic switches
Bauwens, Julien ULg; Tarayre, Cédric ULg; Matteotti, Christel et al

Poster (2012, August)

Wood-feeding termites as Reticulitermes santonensis generally feed on cellulose, hemicelluloses and lignin. However, these opportunistic insects are also able to degrade other carbohydrates, such as ... [more ▼]

Wood-feeding termites as Reticulitermes santonensis generally feed on cellulose, hemicelluloses and lignin. However, these opportunistic insects are also able to degrade other carbohydrates, such as starch. The production of putative endogenous α - amylase has been previously shown in R. flavipes, as the disappearance of the major symbiotic flagellates from the hindgut. Here, we compared enzymatic activities (CMCase, MCCase, xylanase, amylase, α- and β-glucosidase) between different fractions of the digestive tract of starch-, cellulose-, and wood-fed termites. Main compounds of the artificial diets, namely starch or MCC, resulted in differential enzymatic activity. Even the substitution of wood by artificial diets itself seemed to induce changes in enzymatic activities, regardless of the main substrate in the diet, as we observed strong midgut α-glucosidase activity only for artificially-fed termites. Preliminary assays to isolate and characterize enzymes were performed using proteomic methods. [less ▲]

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See detailSelection and cultivation of hydrolytic microorganisms extracted from the digestive tract of the termite Reticulitermes santonensis (3DV.1.55)
Tarayre, Cédric ULg; Bauwens, Julien ULg; Matteotti, Christel ULg et al

Poster (2012, June 21)

Biofuel production can be based on the use of fermentable substrates issued from the hydrolysis of lignocellulosic biomass stemming from agricultural residues and by-products. However, such substrates are ... [more ▼]

Biofuel production can be based on the use of fermentable substrates issued from the hydrolysis of lignocellulosic biomass stemming from agricultural residues and by-products. However, such substrates are not easy to degrade. Enzymes (cellulases, xylanases, etc.) can be used for this purpose and pre-treatments can increase their action by providing more available extremities. The digestive tract of the termite Reticulitermes santonensis contains various microorganisms (bacteria, molds, protists) able to degrade the wood components. These microorganisms act as consortia, leading to a better hydrolysis than in the cow rumen. Our purpose is the isolation of microorganisms from termite guts in order to evaluate their potential for hydrolysis of lignocellulosic materials. This approach led us to isolate and to study a bacteria (Bacillus sp.) displaying a xylanase activity, a mold (Aspergillus sp.) displaying a cellulase activity and a chrysophyte (protist) displaying an amylase activity. [less ▲]

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See detailImpact of Protective Compounds on the Viability, Physiological State and Lipid Degradation of FreezeDried Pseudomonas Fluorescens BTP1 during Storage
Mputu Kanyinda, Jean-Noël ULg; Pierart, C.; Weekers, F. et al

in International Journal of Biotechnology and Biochemistry (2012), 8(4), 17-26

The drying of bacteria remains a major alternative in order to keep them long term. After centrifugation, the bacterial pellet of Pseudomonas fluorescensBTP1 was divided in two fractions one with ... [more ▼]

The drying of bacteria remains a major alternative in order to keep them long term. After centrifugation, the bacterial pellet of Pseudomonas fluorescensBTP1 was divided in two fractions one with protecting compounds (2% glycerol or 5% maltodextrine) and one without and freeze-dried. After freeze drying, powders were sealed in aluminium bag under vacuum and stored at 4 or 20°C. The parameters such as viability, the conductivity and the ratio of polyunsaturated fatty acids/saturated fatty acids were used to investigate the viability of freeze-dried powders during storage. For example cell concentration of powder with glycerol (PG) at CFU/g before storage is 4.109 and after 7 month 2.108 at 4°C and 3,5.107 at 20°C). The ratio of polyunsaturated fatty acids/saturated fatty acids decrease in function of time (e.g. at 4°C the ratios of C18:3 and C18:2 by C16:0 decreases respectively of 0,013 to 0,001 and 0,05 to 0,03 after 60 days of storage). In the present study, flow cytometric analysis was applied to evaluate the state in which the cells are at the end of storage time. We compared the survival results of bacteria obtained by plate count with the flow cytometric analysis results. [less ▲]

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See detailBiofuel by-product for poultry diets
Hissette, Mathias ULg; Destain, Jacqueline ULg; Thewis, André ULg et al

Poster (2012, February 28)

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See detailImpact of glycerol and storage temperature on gluatathione concentration and physiological state of Pseudomonas fluorescens BTP1 freeze-dried
Mputu Kanyinda, Jean-Noël ULg; Pierart, C.; Delvigne, Frank ULg et al

Poster (2012, February 15)

Pseudomonas fluorescens is commonly used as bio-fungicides in agriculture. For this use it requires formulations as either liquid or powder. Formulations have two advantages, storage and transport. Freeze ... [more ▼]

Pseudomonas fluorescens is commonly used as bio-fungicides in agriculture. For this use it requires formulations as either liquid or powder. Formulations have two advantages, storage and transport. Freeze-drying is a commonly used method to preserve bacteria. However, freeze-drying damages the cells, which results in loss of viability. Protective compounds are used to reduce loss of viability during process (freeze-drying and storage). In our study we used flow cytometry analysis to assess the physiological state in which cells are at the end of freeze-drying and Glutathione (GSH) was measured before and during storage. [less ▲]

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See detailContribution to the study of physicochemical and functional properties of hemicelluloses and xylooligosaccharides (XOS) extracted from rapeseed meal
Mertens, Cécile ULg; Paquot, Michel ULg; Destain, Jacqueline ULg et al

Poster (2012, February 08)

Rapeseed meal is one of the main industrial co-products from agriculture in Belgium, with more than 30 000 T produced each year. Beside its agricultural and energetic applications, new paths of ... [more ▼]

Rapeseed meal is one of the main industrial co-products from agriculture in Belgium, with more than 30 000 T produced each year. Beside its agricultural and energetic applications, new paths of valorization are being developed in order to add value to the rapeseed meal, usually by extraction of interesting molecules. In this thesis project, the aimed molecules are hemicelluloses, which are obtained via a global fractionation method that is being developed and optimized, constituting the first part of this project. Indeed, while rapeseed meals are globally already well exploited (mainly for their feed value), rapeseed hemicelluloses have not yet been studied for food application. Yet, hemicelluloses can be used as food additives (thickener, stabilizer, etc) mainly in bakery products. The literature being incomplete regarding the chemical structure of rapeseed hemicelluloses, one of the objectives of this project is to study their physicochemical properties, in regard with their technofunctional properties. Rapeseed hemicelluloses can also be used as raw material to produce an emergent type of prebiotic: xylooligosaccharides (XOS). These molecules can be introduced as well in the food supplement sector. Their production will be achieved by enzymatic hydrolysis, for a polymerization degree between two and five. Thus, the production and the physicochemical and technofunctional characterization of rapeseed XOS constitute the last objective of this project. This thesis is part of the SYNBIOFOR project, which aim is to create new symbiotic ingredients. [less ▲]

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See detailEffect of humic substances on in vitro rooting and acclimatization of Alder (Alnus glutinosa (L.) Gaertn).
Tahiri, Abdelghani ULg; Destain, Jacqueline ULg; Druart, Philippe et al

Poster (2012)

Humic substances (HS) are organic compounds resulting from the physical, chemical and microbiological transformations of organic residues. Present every where in the nature; they are taking part in basic ... [more ▼]

Humic substances (HS) are organic compounds resulting from the physical, chemical and microbiological transformations of organic residues. Present every where in the nature; they are taking part in basic functionalities in any ecosystems involving soils, sediments, waterand landfills. They are heterogeneous and complex carbon macromolecules. Our study aims to compare the effect of HS on in vitro rooting and acclimatization of the Alder species (Alnus glutinosa (L.) Gaertn) as tree growing on river banks or wasted areas. [less ▲]

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See detailPreliminary results on growing tree roots in vitro in presence of humic substances
Tahiri, Abdelghani ULg; Destain, Jacqueline ULg; Druart, Philippe et al

Poster (2012)

Humic substances (HS) are organic compounds resulting from the physical, chemical and microbiological transformations of organic residues. Present everywhere in the nature; they are taking part in basic ... [more ▼]

Humic substances (HS) are organic compounds resulting from the physical, chemical and microbiological transformations of organic residues. Present everywhere in the nature; they are taking part in basic functionalities in any ecosystems involving soils, sediments, water and landfills. They are heterogeneous and complex carbon macromolecules. Our study aims to determine the main biological properties of HS on plant growth in relation to their physicochemical properties. [less ▲]

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See detailLes entérocoques : avantages et inconvénients en biotechnologie (synthèse bibliographique)
Aguilar Galvez, A.; Dubois Dauphin, Robin ULg; Destain, Jacqueline ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2012), 16(1), 67-76

Detailed reference viewed: 21 (6 ULg)