References of "Destain, Jacqueline"
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See detailNovel use of lipopeptide preparations
Deleu, Magali ULg; Brans, Alain; Brasseur, Robert ULg et al

Patent (2004)

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See detailCarbon and nitrogen sources modulate lipase production in the yeast Yarrowia lipolytica
Fickers, Patrick ULg; Nicaud, J. M.; Gaillardin, C. et al

in Journal of Applied Microbiology (2004), 96(4), 742-749

AIMS: To analyse the influence of nitrogen and carbon sources on extracellular lipase production by Yarrowia lipolytica-overproducing mutant in order to optimize its production in large-scale bioreactors ... [more ▼]

AIMS: To analyse the influence of nitrogen and carbon sources on extracellular lipase production by Yarrowia lipolytica-overproducing mutant in order to optimize its production in large-scale bioreactors. METHODS AND RESULTS: The level of lipase production and LIP2 induction, measured using an LIP2-LacZ reporter gene, were compared for different carbon and nitrogen sources and for different concentrations. The localization of the enzyme during growth was also determined by Western blotting analysis using a six-histidine-tagged lipase. SIGNIFICANCE AND IMPACT OF THE STUDY: Tryptone N1 and oleic acid are the most suitable nitrogen and carbon sources for the production of the extracellular lipase by the Y. lipolytica mutant. Higher levels of lipase production were obtained as the tryptone concentration increased in the culture medium. Such a positive correlation was not observed with oleic acid media where the highest lipolytic productivities were obtained in the presence of low concentration. We also demonstrate that in the presence of oleic acid, lipase is cell-bound during the growth phase before being released in the media. CONCLUSIONS: This work provides a better understanding of the mechanism controlling LIP2 expression and, thus, extracellular lipase production in the yeast Y. lipolytica. [less ▲]

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See detailOverproduction of lipase by Yarrowia lipolytica mutants
Fickers, Patrick ULg; Nicaud, J. M.; Destain, Jacqueline ULg et al

in Applied Microbiology & Biotechnology (2003), 63(2), 136-142

Non-genetically modified mutants with increased capacities of extracellular lipase production were obtained from Yarrowia lipolytica strain CBS6303 by chemical mutagenesis. Of the 400 mutants isolated ... [more ▼]

Non-genetically modified mutants with increased capacities of extracellular lipase production were obtained from Yarrowia lipolytica strain CBS6303 by chemical mutagenesis. Of the 400 mutants isolated, LgX64.81 had the highest potential for the development of an industrial lipase production process. This mutant exhibits lipase production uncoupled from catabolite repression by glucose, and a 10-fold increased productivity upon addition of oleic acid. Using a LIP2-LacZ reporter gene, we demonstrate that the mutant phenotype originates from a trans-acting mutation. The glucose uptake capacity of LgX64.81 is reduced 2.5-fold compared to the wild-type-strain, and it exhibits high lipase production on glucose medium. A trans-acting mutation in a gene involved in glucose transport could thus explain this mutant phenotype. [less ▲]

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See detailA novel lox/Cre-mediated multiple gene knockout procedue in the yeast Yarrowia lipolytica
Fickers, Patrick ULg; Le Dal, M.; Gaillardin, C. et al

Poster (2003, May 09)

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See detailMonitoring of the de-esterification and depolymerisation of pectins by nirs.
Sinnaeve, G.; Ciza, A.; Deconinck, T. et al

Poster (2003, May 09)

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See detailCellulose enzymatic availability in Municipal solid waste
Rodriguez, C.; Hiligsmann, Serge ULg; Ongena, MARC ULg et al

in Christensen; Cossu; Stegmann (Eds.) Proceedings Sardinia 03, Ninth International Waste Management and Landfill Symposium (2003)

We recently developed a new and rapid method to evaluate the biological reactivity of cellulose in Municipal Solid Waste (MSW). This test is based on the enzymatic hydrolysis of cellulose in MSW and can ... [more ▼]

We recently developed a new and rapid method to evaluate the biological reactivity of cellulose in Municipal Solid Waste (MSW). This test is based on the enzymatic hydrolysis of cellulose in MSW and can be related to the digestibility of this polymer in MSW. In this work, we showed that the enzymatic cellulose degradation test (ECD) correlated well with a Biochemical Methane Potential (BMP) assay so that the ECD test can be reliably applied to estimate cellulose bioavailability in various waste samples. These tests have also been used to evaluate the influence of some important parameters that could affect the extent and the rate of cellulose degradation in waste. It appeared that cellulose enzymatic hydrolysis is related to the moisture content but no clear relationship could be established with the cellulose content. Moreover, it is shown that acetic acid produced by the anaerobic degradation of organic matter, and responsible for high COD in young leachates, may slow down or inhibit the enzymatic hydrolysis step and the methanogenesis. [less ▲]

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See detailLa problématique de la gestion des déchets ménagers
Thonart, Philippe ULg; Lardinois, M.; Rodriguez, Ch. et al

Conference (2002, November)

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See detailCellulose bioavailability in waste
Rodriguez, Ch.; Hiligsmann, Serge ULg; Lardinois, M. et al

Poster (2002, October)

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See detailLa problématique de la gestion des déchets ménagers en République d'Haïti
Thonart, Philippe ULg; Lardinois, M.; Rodriguez, Ch. et al

Conference (2002, July 01)

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See detailLipase production in Yarrowia lipolytica overproducing mutants.
Fickers, Patrick ULg; Nicaud, J. M.; Destain, Jacqueline ULg et al

Poster (2002, July)

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See detailTraitement biologique des eaux usées : le cas des bacs à graisses.
Destain, Jacqueline ULg; Antoine, P.; Evrard, P. et al

Poster (2002, June)

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See detailScreening of pectolytic microorganisms by near infrared reflectance spectroscopy.
Sinnaeve, G.; Ciza, A.; Deconinck, T. et al

Poster (2001, June)

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See detailCellulose enzymatic availability in waste refuse
Rodriguez, C.; Hiligsmann, Serge ULg; Lardinois, M. et al

in Christensen; Cossu; Stegmann (Eds.) Proceedings Sardinia 01, Eight International Waste Management and Landfill Symposium (2001)

This work presents an original method for testing the biological reactivity of cellulose. This test specifically targets one of the limiting steps of cellulose bioconversion into biogas, i.e. the ... [more ▼]

This work presents an original method for testing the biological reactivity of cellulose. This test specifically targets one of the limiting steps of cellulose bioconversion into biogas, i.e. the cellulose hydrolysis. From this point of view, enzymes-mediated hydrolysis has been performed to evaluate cellulose bioavailability in refuse samples from different layers of an old landfill. The relationship between the cellulose enzymatic accessibility and the lignin contents has also been studied. Similarly, the correlation between cellulose, lignin and humic acid contents has been investigated. On the other hand, moisture content in landfills is considered as one of the most important factors that favours methanogenesis. In this respect, the influence of moisture content on cellulose enzymatic hydrolysis has also been evaluated. [less ▲]

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See detailA pluridisciplinary model to predict municipal landfill life
Hiligsmann, Serge ULg; Rodriguez, C.; Lardinois, M. et al

Conference (2001)

Most degradation processes that occur within municipal wastes landfills result from different microbial activities interacting all along the landfill lifetime and that are influenced by several non ... [more ▼]

Most degradation processes that occur within municipal wastes landfills result from different microbial activities interacting all along the landfill lifetime and that are influenced by several non-biological factors. These complex biological and chemical processes require a multidisciplinary pattern in order to assess and control their environmental impact. Thanks to Walloon Region support, we have developed a pattern or model combining the evolution of different key parameters. Such parameters qualify biogas (composition, production rate), leachate (pH, BOD5, COD, volatile fatty acids, redox potential, nitrogen balance, humic acids...), or solid waste (moisture, settlement, cellulose content...). This paper presents relationships, including our model, between these parameters. Variations of pH, BOD5/COD ratio, sulfate concentration in leachates, gas composition analysis and settlement measures allow to predict landfill age, on a standard scale, since completion. Evaluation of cellulose and water content in refuse as well as settlement degree are useful to estimate the time required for complete biological degradation. [less ▲]

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See detailChemical characterization of Yarrowia lipolytica extracellular lipase.
Destain, Jacqueline ULg; Moreau, B.; Fickers, Patrick ULg et al

Poster (2000, September)

Detailed reference viewed: 9 (4 ULg)