References of "Destain, Jacqueline"
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See detailBioreactor hydrodynamic effect on Escherichia coli physiology: experimental results and stochastic simulations.
Delvigne, Frank ULg; Destain, Jacqueline ULg; Thonart, Philippe ULg

in Bioprocess and Biosystems Engineering (2005), 28(2), 131-7

A microorganism circulating in a bioreactor can be submitted to hydrodynamic conditions inducing a significant effect on its physiology. The mixing time exhibited by the stirred bioreactor and the ... [more ▼]

A microorganism circulating in a bioreactor can be submitted to hydrodynamic conditions inducing a significant effect on its physiology. The mixing time exhibited by the stirred bioreactor and the circulation of microorganisms are both involved in this reacting system. The mixing component determines the intensity of the concentration gradient and the circulation component determines the way in which the microorganism is exposed to this gradient. These two components linked to the experimental evaluation of microbial physiology can be analysed by a structured stochastic model in the case of a partitioned or "scale-down" reactor (SDR). A stochastic model indeed enables to simulate the mixing process as well as the circulation of microorganisms in SDRs. The superimposition of mixing and circulation processes determines the concentration profile experienced by a microorganism in the reactor. In the present case, the glucose concentration experienced by Escherichia coli has been modelled during a fed-batch culture. In this context, the use of a stochastic hydrodynamic model has permitted to point out an interesting feed pulse retardant effect in the SDRs. Nevertheless, the metabolic response of E. coli is not easy to interpret because of the possible simultaneous developments of overflow metabolism and mixed acid fermentation induced by the strong glucose concentration in the reactor. [less ▲]

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See detailTraitement biologique d’un effluent de l’industrie des olives
Alloue; Aldric, Jean-Marc ULg; Destain, Jacqueline ULg et al

in Tribune de l'Eau (La) (2005)

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See detailUtilization of methyloleate in production of microbial lipase
Destain, Jacqueline ULg; Fickers, Patrick ULg; Weekers, F. et al

in Applied Biochemistry and Biotechnology (2005), 121(Spring), 269-277

In this article, we report the development and optimization of an industrial culture medium for the production of extracellular lipase in the yeast Yarrowia lipolytica. Until now olive oil in combination ... [more ▼]

In this article, we report the development and optimization of an industrial culture medium for the production of extracellular lipase in the yeast Yarrowia lipolytica. Until now olive oil in combination with glucose was used as the carbon source and inducer for the production of lipase. Our results demonstrate that methyloleate, a cheap hydrophobic compound, could efficiently substitute olive oil as the inducer and carbon source for lipase production. A new process of lipase production was developed yielding a twofold increase in the level of production compared with the levels in previous reports. [less ▲]

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See detailMethyl Oleate Modulates Lip2 Expression In The Lipolytic Yeast Yarrowia Lipolytica
Fickers, Patrick; Destain, Jacqueline ULg; Thonart, Philippe ULg

in Biotechnology Letters (2005), 27(22),

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See detailUtilization of methyloleate in the production of Yarrowia lipolytica.
Destain, Jacqueline ULg; Moreau, B.; Weekers, F. et al

Poster (2004, May)

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See detailExtracellular catalase production by Aspergillus phoenicis.
Kacem-Chaouche, N.; Meraihi, Z.; Destain, Jacqueline ULg et al

Poster (2004, May)

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See detailCarbon and nitrogen sources modulate lipase production in the yeast Yarrowia lipolytica
Fickers, Patrick ULg; Nicaud, J. M.; Gaillardin, C. et al

in Journal of Applied Microbiology (2004), 96(4), 742-749

AIMS: To analyse the influence of nitrogen and carbon sources on extracellular lipase production by Yarrowia lipolytica-overproducing mutant in order to optimize its production in large-scale bioreactors ... [more ▼]

AIMS: To analyse the influence of nitrogen and carbon sources on extracellular lipase production by Yarrowia lipolytica-overproducing mutant in order to optimize its production in large-scale bioreactors. METHODS AND RESULTS: The level of lipase production and LIP2 induction, measured using an LIP2-LacZ reporter gene, were compared for different carbon and nitrogen sources and for different concentrations. The localization of the enzyme during growth was also determined by Western blotting analysis using a six-histidine-tagged lipase. SIGNIFICANCE AND IMPACT OF THE STUDY: Tryptone N1 and oleic acid are the most suitable nitrogen and carbon sources for the production of the extracellular lipase by the Y. lipolytica mutant. Higher levels of lipase production were obtained as the tryptone concentration increased in the culture medium. Such a positive correlation was not observed with oleic acid media where the highest lipolytic productivities were obtained in the presence of low concentration. We also demonstrate that in the presence of oleic acid, lipase is cell-bound during the growth phase before being released in the media. CONCLUSIONS: This work provides a better understanding of the mechanism controlling LIP2 expression and, thus, extracellular lipase production in the yeast Y. lipolytica. [less ▲]

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See detailOverproduction of lipase by Yarrowia lipolytica mutants
Fickers, Patrick ULg; Nicaud, J. M.; Destain, Jacqueline ULg et al

in Applied Microbiology & Biotechnology (2003), 63(2), 136-142

Non-genetically modified mutants with increased capacities of extracellular lipase production were obtained from Yarrowia lipolytica strain CBS6303 by chemical mutagenesis. Of the 400 mutants isolated ... [more ▼]

Non-genetically modified mutants with increased capacities of extracellular lipase production were obtained from Yarrowia lipolytica strain CBS6303 by chemical mutagenesis. Of the 400 mutants isolated, LgX64.81 had the highest potential for the development of an industrial lipase production process. This mutant exhibits lipase production uncoupled from catabolite repression by glucose, and a 10-fold increased productivity upon addition of oleic acid. Using a LIP2-LacZ reporter gene, we demonstrate that the mutant phenotype originates from a trans-acting mutation. The glucose uptake capacity of LgX64.81 is reduced 2.5-fold compared to the wild-type-strain, and it exhibits high lipase production on glucose medium. A trans-acting mutation in a gene involved in glucose transport could thus explain this mutant phenotype. [less ▲]

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See detailA novel lox/Cre-mediated multiple gene knockout procedue in the yeast Yarrowia lipolytica
Fickers, P.; Le Dal, M.; Gaillardin, C. et al

Poster (2003, May 09)

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See detailMonitoring of the de-esterification and depolymerisation of pectins by nirs.
Sinnaeve, G.; Ciza, A.; Deconinck, T. et al

Poster (2003, May 09)

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See detailCellulose enzymatic availability in Municipal solid waste
Rodriguez, C.; Hiligsmann, Serge ULg; Ongena, MARC ULg et al

in Christensen; Cossu; Stegmann (Eds.) Proceedings Sardinia 03, Ninth International Waste Management and Landfill Symposium (2003)

We recently developed a new and rapid method to evaluate the biological reactivity of cellulose in Municipal Solid Waste (MSW). This test is based on the enzymatic hydrolysis of cellulose in MSW and can ... [more ▼]

We recently developed a new and rapid method to evaluate the biological reactivity of cellulose in Municipal Solid Waste (MSW). This test is based on the enzymatic hydrolysis of cellulose in MSW and can be related to the digestibility of this polymer in MSW. In this work, we showed that the enzymatic cellulose degradation test (ECD) correlated well with a Biochemical Methane Potential (BMP) assay so that the ECD test can be reliably applied to estimate cellulose bioavailability in various waste samples. These tests have also been used to evaluate the influence of some important parameters that could affect the extent and the rate of cellulose degradation in waste. It appeared that cellulose enzymatic hydrolysis is related to the moisture content but no clear relationship could be established with the cellulose content. Moreover, it is shown that acetic acid produced by the anaerobic degradation of organic matter, and responsible for high COD in young leachates, may slow down or inhibit the enzymatic hydrolysis step and the methanogenesis. [less ▲]

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See detailLa problématique de la gestion des déchets ménagers
Thonart, Philippe ULg; Lardinois, M.; Rodriguez, Ch. et al

Conference (2002, November)

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See detailCellulose bioavailability in waste
Rodriguez, Ch.; Hiligsmann, Serge ULg; Lardinois, M. et al

Poster (2002, October)

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