References of "Destain, Jacqueline"
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See detailFormulation de la lipase de Yarrowia lipolytica
Alloue Waze, M.; Destain, Jacqueline ULg; Thonart, Philippe ULg

Poster (2006, September)

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See detailSelection of bacteriocin-producing lactic acid bacteria from Senegal traditional fermented food
Diop, M.; Dubois Dauphin, Robin ULg; Tine, E. et al

Poster (2006, September)

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See detailImmobilisation de la lipase de Yarrowia lipolytica.
Kabran, P.; Alloue, M.; Destain, Jacqueline ULg et al

Poster (2006, September)

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See detailProduction and down-stream processing of an extracellular lipase from the yeast Yarrowia lipolytica
Fickers, Patrick ULg; Ongena, MARC ULg; Destain, Jacqueline ULg et al

in Enzyme & Microbial Technology (2006), 38(6), 756-759

Lipase constitutes an interesting class of enzyme with many biotechnological applications. However, the development of a fruitful process must be set up to obtained a product compatible with the ... [more ▼]

Lipase constitutes an interesting class of enzyme with many biotechnological applications. However, the development of a fruitful process must be set up to obtained a product compatible with the industrial and commercial needs. Here, we report the development of such a process for the extracellular lipase secreted by the yeast Yarrowia lipolytica. The enzyme production, carried out in a 2000 L bioreactor, led to a lipase activity of approximately 1100 U mL(-1) after 53 h of fermentation. The post-culture treatment, consisting of a centrifugation, a filtration and an ultra-filtration steps, led to 15-fold volume reduction and a 8-fold increased of the lipase activity. Finally, addition of 12% of milk powder and 3% of gum arabic before spray-drying dehydration led to a stable powder with a lipolytic activity of 37,500 U g D.W.(-1). (c) 2005 Elsevier Inc. All rights reserved. [less ▲]

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See detailBacteriocin Activity By Lactobacillus Curvatus Cwbi-B28 To Inactivate Listeria Monocytogenes In Cold-Smoked Salmon During 4 Degrees C Storage
Ghalfi, H.; Allaoui, A.; Destain, Jacqueline ULg et al

in Journal of Food Protection (2006), 69(5),

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See detailStochastic models to study the impact of mixing on a fed-batch culture of Saccharomyces cerevisiae.
Delvigne, Frank ULg; Lejeune, Annick ULg; Destain, Jacqueline ULg et al

in Biotechnology Progress (2006), 22(1), 259-69

The mechanisms of interaction between microorganisms and their environment in a stirred bioreactor can be modeled by a stochastic approach. The procedure comprises two submodels: a classical stochastic ... [more ▼]

The mechanisms of interaction between microorganisms and their environment in a stirred bioreactor can be modeled by a stochastic approach. The procedure comprises two submodels: a classical stochastic model for the microbial cell circulation and a Markov chain model for the concentration gradient calculus. The advantage lies in the fact that the core of each submodel, i.e., the transition matrix (which contains the probabilities to shift from a perfectly mixed compartment to another in the bioreactor representation), is identical for the two cases. That means that both the particle circulation and fluid mixing process can be analyzed by use of the same modeling basis. This assumption has been validated by performing inert tracer (NaCl) and stained yeast cells dispersion experiments that have shown good agreement with simulation results. The stochastic model has been used to define a characteristic concentration profile experienced by the microorganisms during a fermentation test performed in a scale-down reactor. The concentration profiles obtained in this way can explain the scale-down effect in the case of a Saccharomyces cerevisiae fed-batch process. The simulation results are analyzed in order to give some explanations about the effect of the substrate fluctuation dynamics on S. cerevisiae. [less ▲]

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See detailInvolvement of fengycin-type lipopeptides in the multifaceted biocontrol potential of Bacillus subtilis
Ongena, MARC ULg; Jacques, P.; Toure, Y. et al

in Applied Microbiology & Biotechnology (2005), 69(1), 29-38

In this work, the potential of Bacillus subtilis strain M4 at protecting plants against fungal diseases was demonstrated in different pathosystems. We provide evidence for the role of secreted ... [more ▼]

In this work, the potential of Bacillus subtilis strain M4 at protecting plants against fungal diseases was demonstrated in different pathosystems. We provide evidence for the role of secreted lipopeptides, and more particularly of fengycins, in the protective effect afforded by the strain against damping-off of bean seedlings caused by Pythium ultimum and against gray mold of apple in post-harvest disease. This role was demonstrated by the strong biocontrol activity of lipopeptide-enriched extracts and through the detection of inhibitory quantities of fengycins in infected tissues. Beside such a direct antagonism of the pathogen, we show that root pre-inoculation with M4 enabled the host plant to react more efficiently to subsequent pathogen infection on leaves. Fengycins could also be involved in this systemic resistance-eliciting effect of strain M4, as these molecules may induce the synthesis of plant phenolics involved in or derived from the defense-related phenylpropanoid metabolism. Much remains to be discovered about the mechanisms by which Bacillus spp suppress disease. Through this study on strain M4, we reinforce the interest in B. subtilis as a pathogen antagonist and plant defense-inducing agent. The secretion of cyclic fengycin-type lipopeptides may be tightly related to the expression of these two biocontrol traits. [less ▲]

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See detailSynthese enzymatique d’esters de sucre au départ d’huiles végétales
Detry, J.; Destain, Jacqueline ULg; Lognay, Georges ULg et al

Poster (2005, October 14)

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See detailSugar esters lipase mediated synthesis from vegetable oils
Destain, Jacqueline ULg; Detry, Jean; Lognay, Georges ULg et al

Scientific conference (2005, September 19)

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See detailSugar esters lipase mediated synthesis from vegetable oils
Destain, Jacqueline ULg; Detry, J.; Lognay, Georges ULg et al

Poster (2005, September)

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See detailFormulation solide de la lipase de Yarrowia lipolytica
Alloue, M.; Destain, Jacqueline ULg; Thonart, Philippe ULg

Poster (2005, March 05)

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See detailInvolvement of hexokinase HXK1 in glucose catabolite repression of LIP2 encoding extracellular lipase in the yeast Yarrowia lipolytica
Fickers, Patrick ULg; Nicaud, J. M.; Destain, Jacqueline ULg et al

in Current Microbiology (2005), 50(3), 133-137

The yeast Yarrowia lipolytica produces an extracellular lipase encoded by the LIP2 gene. However, very little is known about the mechanisms controlling its expression, especially on glucose media. In this ... [more ▼]

The yeast Yarrowia lipolytica produces an extracellular lipase encoded by the LIP2 gene. However, very little is known about the mechanisms controlling its expression, especially on glucose media. In this work, the involvement of hexokinase Hxk1 in the glucose catabolite repression of LIP2 was investigated in a lipase overproducing mutant less sensitive to glucose repression. This mutant has a reduced capacity to phosphorylate hexose compared with the wild-type strain, but no differences could be observed between the HXK1 sequences in the two isolates. This suggested that the reduced phosphorylating activity of the mutant strain probably resulted from a modification in the level of HXK1 expression. However, overexpression of the HXK1 gene in this mutant led to a decrease of both LIP2 induction and extracellular lipase activity, suggesting that the hexokinase is involved in the glucose catabolite repression of LIP2 in Y lipolytica. [less ▲]

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See detailSelection of new over-producing derivatives for the improvement of extracellular lipase production by the non-conventional yeast Yarrowia lipolytica
Fickers, Patrick ULg; Fudalej, F.; Nicaud, J. M. et al

in Journal of Biotechnology (2005), 115(4), 379-386

The non-conventional yeast Yarrowia lipolytica produces an extracellular lipase encoded by the LIP2 gene. Mutant strains with enhanced productivity were previously obtained either by chemical mutagenesis ... [more ▼]

The non-conventional yeast Yarrowia lipolytica produces an extracellular lipase encoded by the LIP2 gene. Mutant strains with enhanced productivity were previously obtained either by chemical mutagenesis or genetic engineering. In this work, we used one of these mutants, named LgX64.81 to select new overproducing strains following by amplification of the LIP2 gene. We also developed a process for lipase production in bioreactors and compared lipase production levels in batch and fed-batch cultures. Batch culture led to a lipase production of 26 450 U ml(-1) in a media containing olive oil and tryptone as carbon and nitrogen sources. Feeding of a combination of tryptone and olive oil at the end of the exponential growth phase yielded to lipase activity of 158 246 U ml(-1) after 80 h of cultivation. In addition this production system developed for the extracellular lipase could also be applied for other heterologous protein production since we have demonstrated that LgX64.81 is an interesting alternative host strain. (C) 2004 Elsevier B.V. All rights reserved. [less ▲]

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See detailThe two-phase water/silicon oil bioreactor prospects in off-gas treatment
Aldric, Jean-Marc ULg; Destain, Jacqueline ULg; Thonart, Philippe ULg

in Applied Biochemistry and Biotechnology (2005), 121(Spring), 707-719

Research was carried out to develop a biphasic biologic reactor able to clean the gas effluents polluted by volatile organic compounds. Initially, Rhodococcus erythropolis T 902.1 was selected on the ... [more ▼]

Research was carried out to develop a biphasic biologic reactor able to clean the gas effluents polluted by volatile organic compounds. Initially, Rhodococcus erythropolis T 902.1 was selected on the basis of its capacity to degrade isopropylbenzene (IPB). The effect of gas flow and IPB concentration on the biodegadation of IPB was evaluated. The results show that the use of silicon oil allows large quantities of IPB to be absorbed within the medium of biologic abatement. On the other hand, the biodegradation rate was directly correlated to the inlet flow of IPB. Thus, the reactor presents interesting opportunities for the biologic treatment of gas effluents. [less ▲]

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