References of "Destain, Jacqueline"
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See detailPhysiological and bio-functional properties of gum arabic: a notable interest for certain human diseases
Eloundou Mballa, Pierre; Goffin, Dorothée ULg; Destain, Jacqueline ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (in press)

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See detailWood digestion in lower termites: multidisciplinary approaches based on differential feeding
Bauwens, Julien ULg; Brasseur, Catherine ULg; Tarayre, Cédric ULg et al

Poster (2014, December)

Termites digestive tract and hindgut especially still holds many secrets despites hundreds of years of research. The complexity of the symbiotic microbial community and the contrast of physio-chemical ... [more ▼]

Termites digestive tract and hindgut especially still holds many secrets despites hundreds of years of research. The complexity of the symbiotic microbial community and the contrast of physio-chemical environments found in lower termites paunch are potentially the key point to explain the efficiency of ligno-cellulose digestion. Contribution of advancing technologies accelerates the progress of our knowledge in this field. Here, we present multiple approaches combining old and recent techniques used to highlight the effect of ligno-cellulosic compounds on termite gut and the role of populations from the symbiotic microbial community. Termites Reticulitermes flavipes (Kollar) submitted to various artificial diets showed variations in flagellates populations profile and enzymatic activities. Differential protein expression was investigated using 2D-DIGE MALDI-TOF-TOF and 2D-LC-MS/MS using high resolution orbitrap analyzer. Results from both proteomic experiments tend to support each-other and bring complementary points of view. The gel-free analysis resulted in highly contrasted identification of enzymes involved in ligno-cellulose digestion and metabolism. Finally, differential feeding experiments leaded to in vivo selection of different symbiotic communities. These communities were characterized following some metabolism assays and allowed the cultivation of diverse microbial consortia using media closely related to the respective artificial diets. This work provides relevant data on termite and associated microbial community response to alimentary diets. [less ▲]

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See detailIsolation and cultivation of xylanolytic and cellulolytic Sarocladium kiliense and Trichoderma virens from the gut of the termite Reticulitermes santonensis
Tarayre, Cédric ULg; Bauwens, Julien ULg; Brasseur, Catherine ULg et al

in Environmental Science & Pollution Research (2014)

The purpose of this work was the isolation and cultivation of cellulolytic and xylanolytic microorganisms extracted from the gut of the lower termite Reticulitermes santonensis. Microcrystalline cellulose ... [more ▼]

The purpose of this work was the isolation and cultivation of cellulolytic and xylanolytic microorganisms extracted from the gut of the lower termite Reticulitermes santonensis. Microcrystalline cellulose (with and without lignin) and beech wood xylan were used as diets instead of poplar wood in order to select cellulose and hemicellulose-degrading fungi. The strain Sarocladium kiliense (Acremonium kiliense) CTGxxyl was isolated from the termites fed on xylan, while the strain Trichoderma virens CTGxAviL was isolated from the termites fed on cellulose (with and without lignin). Both molds were cultivated in liquid media containing different substrates: agro-residues or purified polymers. S. kiliense produced maximal β-glucosidase, endo-1,4-β-D-glucanase, exo-1,4-β-D-glucanase and endo-1,4-β-D-xylanase activities of 0.103, 3.99, 0.53, and 40.8 IU/ml, respectively. T. virens produced maximal β-xylosidase, endo-1,4-β-D-glucanase, exo-1,4-β-D-glucanase, and endo-1,4-β-D-xylanase activities of 0.38, 1.48, 0.69, and 426 IU/ml. The cellulase and the xylanase of S. kiliense, less common than T. virens, were further investigated. The optimal activity of the xylanase was observed at pH 9–10 at 60 °C. The cellulase showed its maximal activity at pH 10, 70 °C. Zymography identified different xylanases produced by both molds, and some fragment sizes were highlighted: 35, 100, and 170 kDa for S. kiliense and 20, 40, 80, and 170 kDa for T. virens. In both cases, endo-1,4-β-D-xylanase activitieswere confirmed through mass spectrometry. [less ▲]

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See detailMultidisciplinary approaches and fractionations to study lower termite symbiotic system and ligno-cellulose digestion
Bauwens, Julien ULg; Brasseur, Catherine ULg; Tarayre, Cédric ULg et al

Poster (2014, August)

Wood-feeding termites are a considerable source of enzymes active on ligno-cellulosic compounds. These enzymes are produced by the termite host and some representatives of its symbiotic microbial ... [more ▼]

Wood-feeding termites are a considerable source of enzymes active on ligno-cellulosic compounds. These enzymes are produced by the termite host and some representatives of its symbiotic microbial community, and are of particular interest in regard second generation biofuel. However, the complexity of microbial interactions renders micro-organisms isolation very difficult. Culture-independent methods permitted to gather a large amount of data and to understand a little more the role of each microbial population, particularly the prokaryotes. Proteomics allows working on the final product of gene expression, and corresponds more to the real operation of the digestive system. In order to investigate such a complex system, it is necessary to use multidisciplinary approaches and to fractionate this system. Zymography or affinity chromatography are used in parallel of routine proteomics techniques such as two-dimensional gel electrophoresis associated to MALDI-TOF mass spectrometry and nano-LC ESI-MS/MS. We used an artificial-diet based rearing to induce changes in microbial population balance. We performed preliminary assay to investigate the glycosylated proteome in the hindgut of a lower termite, using Multi-Lectin Affinity Chromatography (M-LAC) and enzymatic activity of harvested fractions was assessed on cellulosic substrates. [less ▲]

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See detailTest of humic substances on in vitro roots initiation using isolated leaves of woody species
Tahiri, Abdelghani ULg; Destain, Jacqueline ULg; Thonart, Philippe ULg et al

Poster (2014, May 15)

Arise from the chemical and biological degradation of plant and animal residues and from the synthetic activities of microorganisms in the soil, humic substances (HS) are natural heterogeneous aromatic ... [more ▼]

Arise from the chemical and biological degradation of plant and animal residues and from the synthetic activities of microorganisms in the soil, humic substances (HS) are natural heterogeneous aromatic and organic compounds. These substances are chemically complex with no clearly defined chemical structure, although generalized models have been proposed and they can be divided into fractions of humic acids, fulvic acids and humins depending on their solubility in water as a function of the pH. The stimulation of plant growth and development by HS are the activities that have attracted the attention of many scientists. They influence plant productivity directly by the stimulation of biochemical and metabolic processes or indirectly through the modification of soil characteristics and microflora activities. All together, these properties mainly affect root architecture. By inducing root hairs proliferation, differentiating root cells and enhancing lateral root emergence, an increase of the total root biomass is observed. Experiments targeting the rooting stages in absence of interferences were conducted in vitro using HS extracted from landfill leachate and a stable commercial formulation (“Humifirst” from TRADECORP company: 12% humic acid and fulvic acid 3%) issued from leonardite. Shoots and leaves explants of silver birch (Betula pendula Roth) and black alder (Alnus glutinosa L. Gaertn) vitro-plants were treated with 10 ppm leachate HS and 100 ppm Humifirst HS for 5 days during the rooting induction/initiation phase. The treated explants were then transferred into elongation medium containing only nitrate calcium for 4 weeks. The results show that application of HS during the root induction/initiation phase did not significantly influence root growth of both species in comparison with control explants. [less ▲]

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See detailIsolation of an amylolytic chrysophyte, Poterioochromonas sp. from the digestive tract of the termite R. santonensis
Tarayre, Cédric ULg; Bauwens, Julien ULg; Brasseur, Catherine ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2014), 18(1),

The aim of this work was the isolation and cultivation of amylolytic protists living in the digestive tract of the termite Reticulitermes santonensis (Feytaud). A chrysophyte identified as ... [more ▼]

The aim of this work was the isolation and cultivation of amylolytic protists living in the digestive tract of the termite Reticulitermes santonensis (Feytaud). A chrysophyte identified as Poterioochromonas sp. was isolated in a special medium containing rice grains as a source of carbon and nitrogen. Then, the protist was grown in a medium containing starch as a carbon source, tryptone, and a phosphate buffer at different pH values (5, 6 and 7). Yeast extract was added or not. Ciprofloxacin was used to avoid the bacterial development. Other antibiotics were also tested but showed an inhibitive effect on the growth of Poterioochromonas sp. Yeast extract allowed reaching 1.9 (pH 5), 2.3 (pH 6) and 2.2 (pH 7) times higher final cell concentrations, and 2.8 (pH 5), 2.8 (pH 6) and 2.2 (pH 7) times higher biomass yields. The starch concentration did not decrease in the medium until 3 and 4 days of culture, with and without yeast extract, respectively. Eight days of culture were necessary for hydrolyzing the starch completely, with and without yeast extract. Maltose and maltotriose were detected in the culture media and were hydrolyzed progressively. Maximal maltose concentrations were 0.68, 0.66 and 0.51 g.l-1 in the medium containing yeast extract. Maltotriose concentrations were only 0.17, 0.14 and 0.12 g.l-1. Other glucose oligomers were also detected but in lower quantities. It was determined that the protist developed a weak amylase activity, particularly at a weakly acidic pH (5-6). Such a pH also allowed a better growth of the protist. A maximal amylase activity of 112 nkat.l-1 was measured with yeast extract at pH 5. No other enzymatic activity (protease, cellulase or xylanase) was detected except amylase. The degradation products of starch which were obtained by enzymatic hydrolysis allow the identification of α-amylase, amyloglucosidase and possibly β-amylase activities. [less ▲]

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See detailValorisation et propriétés des substances humiques des lixiviats de décharge
Tahiri, Abdelghani ULg; Destain, Jacqueline ULg; Thonart, Philippe ULg et al

Conference (2013, December 18)

Des expérimentations ont été menées dans notre laboratoire en utilisant des substances humiques (SH) extraites des lixiviats de cEntre d’Enfouissemnt Techique et des une formulation stable de SH « ... [more ▼]

Des expérimentations ont été menées dans notre laboratoire en utilisant des substances humiques (SH) extraites des lixiviats de cEntre d’Enfouissemnt Techique et des une formulation stable de SH « Humifirst » (12%d’acides humiques et 3% d’acides fulviques) de la compagnie TRADECORP s.a. (Espagne) commercialisée sous l’appelation Humifirst en vue d’en étudier leurs effets sur le développement du système racinaire de vitro-plants de bouleau et d’aulne. Les résultats montrent qu‘un traitement à faible concentration (10ppm) pendant la phase d’élongation des racines affecte la formation des racines latérales formée en présence de SH de lixiviat avec une intensité variable selon l’espèce testée. [less ▲]

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See detailShort communication - Isolation of amylolytic, xylanolytic, and cellulolytic microorganisms extracted from the gut of the termite Reticulitermes santonensis by means of a micro-aerobic atmosphere
Tarayre, Cédric ULg; Brognaux, Alison ULg; Bauwens, Julien ULg et al

in World Journal of Microbiology & Biotechnology (2013)

The aim of this work was to isolate enzyme-producing microorganisms from the tract of the termite Reticulitermes santonensis. The microorganisms were extracted from the guts and anaerobic (CO2 or CO2/H2 ... [more ▼]

The aim of this work was to isolate enzyme-producing microorganisms from the tract of the termite Reticulitermes santonensis. The microorganisms were extracted from the guts and anaerobic (CO2 or CO2/H2) and micro-aerobic atmospheres were used to stimulate growth. Three different strategies were tried out. First, the sample was spread on Petri dishes containing solid media with carboxymethylcellulose, microcrystalline cellulose or cellobiose. This technique allowed us to isolate two bacteria: Streptomyces sp. strain ABGxAviA1 and Pseudomonas sp. strain ABGxCellA. The second strategy consisted in inoculating a specific liquid medium containing carboxymethylcellulose, microcrystalline cellulose, or cellobiose. The samples were then spread on Petri dishes with the same specific medium containing carboxymethylcellulose, microcrystalline cellulose, or cellobiose. This led to the isolation of the mold Aspergillus sp. strain ABGxAviA2. Finally, the third strategy consisted in heating the first culture and spreading samples on agar plates containing rich medium. This led to the isolation of the bacterium Bacills subtilis strain ABGx. All those steps were achieved in controlled atmospheres. The four enzyme-producing strains which were isolated were obtained by using a micro-aerobic atmosphere. Later, enzymatic assays were performed on the four strains. Streptomyces sp. strain ABGxAviA1 was found to produce only amylase, while Pseudomonas sp. strain ABGxCellA was found to produce β-glucosidase as well. Aspergillus sp. strain ABGxAviA2 showed β-glucosidase, amylase, cellulase, and xylanase activities. Finally, Bacillus subtilis strain ABGx produced xylanase and amylase. [less ▲]

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See detailPrésentation des projets Interreg IVB BIOREFINE et RENEW
Tarayre, Cédric ULg; Delvigne, Frank ULg; Destain, Jacqueline ULg et al

Conference (2013, October 22)

Les projets BioRefine et Renew sont financés par le programme de coopération européen Interreg IVB. Leur but est de promouvoir les transferts de compétences, d'informations et de matières entre les ... [more ▼]

Les projets BioRefine et Renew sont financés par le programme de coopération européen Interreg IVB. Leur but est de promouvoir les transferts de compétences, d'informations et de matières entre les différents pays de la zone européenne du Nord-Ouest. Le projet BioRefine a pour but de récupérer les nutriments (N, P et K) ainsi que les éléments traces métalliques à partir de déchets bien spécifiques: le fumier, le lisier, les digestats de méthanisation et les boues de stations d'épuration. Cette récupération requiert l'élaboration de procédés industriels mis en place après une étude en laboratoire. Le projet Renew, lui, est beaucoup plus général. En ce qui concerne le rôle de Gembloux Agro-Bio Tech dans le projet, le travail sera essentiellement focalisé sur la production d'acide succinique à partir de déchets. Cette production sera assurée par la souche bactérienne Actinobacillus succinogenes. [less ▲]

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See detailStudy of the sorption behavior of Hibscus sabdariffa anthocyanins on a macroporous resin
Beye, Cheikh ULg; Souk Tounkara, Lat; Destain, Jacqueline ULg et al

in Journal of Food Process Engineering (2013), 36(5), 579-590

The adsorption of Hibiscus sabdariffa sp. anthocyanins onto a macroporous resin was studied in laboratory experiments. The anthocyanin aqueous extracts obtained from the calyces were used in agitated ... [more ▼]

The adsorption of Hibiscus sabdariffa sp. anthocyanins onto a macroporous resin was studied in laboratory experiments. The anthocyanin aqueous extracts obtained from the calyces were used in agitated batch experiments with a macroporous resin. The adsorption kinetics and isotherms of H. sabdariffa anthocyanins on a selected macroporous resin was studied to determine the parameters that have to be optimized to recover the anthocyanins from aqueous extracts of the calyces. The adsorption kinetic experimental data were fit into a pseudo second order kinetic model, which was then used to determine several parameters such as the contact time necessary to reach equilibrium as well as the maximum adsorption capacity. The best fit for the equilibrium adsorption isotherm experimental data was obtained with the Langmuir isotherm model. Based on qualitative and quantitative interpretation of the experimental data, the resin that was tested here appeared to have good adsorption capacity for the anthocyanins even though the necessary time to reach equilibrium was particularly long. Three aspects of the desorption of anthocyanins from the resin were studied: the speed of release of the anthocyanins (desorption kinetics), the extent of the anthocyanin desorption depending on the adsorption contact time and finally, the influence of the mobile phase's characteristics [less ▲]

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See detailSuitability of the Weibull 4-parameters model to predict the induction phase of α-amylase production during red sorghum malting when a steep in dilute NaOH is used prior to a resteep in a Bacillus subtilis-S499 based treatment
Bwanganga Tawaba, Jean-Claude ULg; Benjamin Pondo Kouadio; Malumba Kamba, Paul ULg et al

in Journal of the Institute of Brewing (2013)

In previous studies, Bacillus subtilis has been used to control mould growth during red sorghum malting. The use of this biocontrol in steeping liquor has been optimized with some success and the combined ... [more ▼]

In previous studies, Bacillus subtilis has been used to control mould growth during red sorghum malting. The use of this biocontrol in steeping liquor has been optimized with some success and the combined use of a 0.2% NaOH steep, followed by a resteep in a Bacillus subtilis-based biocontrol, has been proposed. The sharpness and variability of the β-amylase peak and the higher levels of β-glucanase obtained in the presence of B. subtilis cells were highlighted. In this work, the suitability of the Weibull 4 Parameters Model to predict sorghum malt α-amylase activity during the enzyme induction stage of red sorghum germination has been compared with those of a 2nd Order Polynomial Model and a General Linear Model. Results obtained showed that the Weibull 4 Parameters Model could be used to predict α-amylase activity, with significant goodness of fit when compared to the 2nd Order Polynomial Model and to the General Linear Model. The effects of steeping treatment (combined use of 0.2% NaOH and Bacillus subtilis S499 starters) and the germination temperature are presented. When the Bacillus subtilis culture used as a starter was diluted, the treatment efficacy to develop α-amylase activity was lost. This study also showed that the germination temperature affected the α-amylase activity rate increase during the induction phase. [less ▲]

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See detailIsolation and Cultivation of a Xylanolytic Bacillus subtilis Extracted from the Gut of the Termite Reticulitermes santonensis
Tarayre, Cédric ULg; Brognaux, Alison ULg; Brasseur, Catherine ULg et al

in Applied Biochemistry and Biotechnology (2013)

The aim of this work was the isolation of xylanolytic microorganisms from the digestive tract of the termite Reticulitermes santonensis. The reducing sugars released after the hydrolysis of xylans can be ... [more ▼]

The aim of this work was the isolation of xylanolytic microorganisms from the digestive tract of the termite Reticulitermes santonensis. The reducing sugars released after the hydrolysis of xylans can be further fermented to provide bioethanol. A xylanolytic strain of Bacillus subtilis was isolated from the hindgut of the termite and displayed amylase and xylanase activities. The bacterium was grown on media containing agricultural residues: wheat bran, wheat distiller’s grains, and rapeseed oil cake. Wheat bran led to the highest induction of xylanase activity, although the development of the strain was less fast than in the other media. It was possible to reach maximal xylanase activities of 44.3, 33.5, and 29.1 I.U./ml in the media containing wheat bran, wheat distiller’s grains, and rapeseed oil cake, respectively. Mass spectrometry identified a wide range of xylose oligomers, highlighting an endoxylanase activity. The enzyme was stable up to 45 °C and displayed an optimal pH close to 8. [less ▲]

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See detailIsolation and cultivation of cellulolytic and xylanolytic bacteria and molds extracted from the gut of the termite Reticulitermes santonensis (3DV.1.14)
Tarayre, Cédric ULg; Bauwens, Julien ULg; Mattéotti, Christel et al

Poster (2013, June)

Biofuel production can be based on the use of agro-residues, consisting in a complex lignocellulosic structure which is not easily hydrolysable. The digestive tract of the termite Reticulitermes ... [more ▼]

Biofuel production can be based on the use of agro-residues, consisting in a complex lignocellulosic structure which is not easily hydrolysable. The digestive tract of the termite Reticulitermes santonensis contains a diversified microflora able to hydrolyze the wood components. Bacteria, molds and protists form efficient consortia, able to break the lignocellulosic complex by producing enzymes, such as xylanases and cellulases. Our purpose is the isolation of microbial strains from termite guts in order to evaluate their potential for hydrolysis of lignocellulosic materials. Termites were fed using different diets chosen to improve the xylanolytic and cellulolytic microflora: wood, microcristalline cellulose (added with lignin or not), α-cellulose (added with lignin or not) and birchwood xylan. Then, dissections were realized to isolate the potential xylanolytic and cellulolytic strains. This approach led us to isolate and to study several strains of bacteria (Bacillus sp. strain CTGx and Chryseobacterium sp. strain CTGx) and molds (Trichoderma virens strain CTGx and Sarocladium kiliense strain CTGx). These microorganisms were able to hydrolyze starch, xylan, cellulose, carboxymethylcellulose, esculin, β-glucan and Whatman® filter paper. They can produce glucose and xylose monomers and oligomers which can be further fermented to produce bioethanol. [less ▲]

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See detailResearch of New Enzyme Producing Strains in the Gut of the Termite Reticulitermes santonensis
Tarayre, Cédric ULg; Bauwens, Julien ULg; Mattéotti, Christel et al

Poster (2013, June)

Termites contain a complex microflora inside of their guts. Inferior termites contain bacteria, mycetes and protists that interact to degrade vegetable components. These strains act as consortia to break ... [more ▼]

Termites contain a complex microflora inside of their guts. Inferior termites contain bacteria, mycetes and protists that interact to degrade vegetable components. These strains act as consortia to break natural materials by secreting various enzymes. Our aim was the isolation and cultivation of microorganisms in order to produce new enzymes that can be further used in green chemistry. Termites were fed with different diets: pinewood, microcristalline cellulose (added with lignin or not), α-cellulose (added with lignin or not) and birchwood xylan. Then, dissections were realized to isolate interesting strains. All the microorganisms were subjected to enzyme assays. That technique allowed us to isolate and to cultivate various strains of bacteria, molds and protists. Three strains of bacteria, two strains of molds and one strain of protist were isolated and displayed different enzymatic activities. The bacteria Bacillus subtilis strain ABGx, Bacillus sp. strain CTGx and Chryseobacterium sp. strain CTGx displayed amylase, cellulase and xylanase activities. The molds Trichoderma virens strain CTGx and Sarocladium kiliense strain CTGx were also able to produce those enzymes. However, the protist Poterioochromonas sp. was found to produce only amylase. In conlusion, the termite gut is a complex culivation medium that provides a habitat for many microorganisms that show interesting enzymatic activities. [less ▲]

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