References of "Desmecht, Daniel"
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See detailEvaluation of systolic function in calves by Doppler echocardiography: preliminary results
Brihoum, M; Rollin, Frédéric ULg; Desmecht, Daniel ULg et al

in Proceedings of the XXIVth World Buiatrics Congress (2006)

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See detailExpression of the interferon-alpha/beta-inducible bovine Mx1 dynamin interferes with replication of rabies virus
Leroy, Michael; Pire, Grégory; Baise, Etienne ULg et al

in Neurobiology of Disease (2006), 21(3), 515-521

Rabies is a fatal anthropozoonotic viral infection of the central nervous system that remains a serious public health problem in many countries. As several animal cases of spontaneous survival to ... [more ▼]

Rabies is a fatal anthropozoonotic viral infection of the central nervous system that remains a serious public health problem in many countries. As several animal cases of spontaneous survival to infection were reported and because type 1 interferons were shown to protect against the virus, it was suggested that innate resistance mechanisms exist. Among the antiviral proteins that are synthesized in response to interferon-alpha/beta stimulation, Mx proteins from several species are long known to block the replication of vesicular stomatitis virus (VSV). As both VSV and rabies virus belongs to the Rhabdoviridae family, this study was started with the aim to establish whether the anti-VSV activity of a mammalian Mx protein could be extended to rabies virus. This question was addressed by inoculating the virus onto a bovine Mx1 or human MxA-expressing Vero cell clone. Plaque formation was unambiguously blocked, and viral yields were reduced 100- to 1000-fold by bovine Mx1 expression for both SAG2 and SADB19 viral strains. In opposition, only SAG2 strain could be inhibited by the expression of human MxA protein. The effect of both proteins expression was then evaluated at the viral protein expression level. Again, boMx1 was able to repress protein expression in both strain, whereas only SAG2 proteins were inhibited in human MxA-expressing cells. These results suggest that protection conferred by interferon-alpha/beta against rabies could be, at least partially, attributable to the Mx pathway. Alternatively, bovine Mx1 could be unique in its ability to repress rabies virus which, if confirmed in vivo, would open an avenue for the development of new antirabies therapeutic strategies. [less ▲]

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See detailDifferential resistance/susceptibility patterns to pneumovirus infection among inbred mouse strains
Bui Tran Anh, Dao; Faisca, Rui-Pedro; Desmecht, Daniel ULg

in American Journal of Physiology - Lung Cellular and Molecular Physiology (2006), 291

Respiratory syncytial virus (RSV) is a prominent cause of airway morbidity in children under 1 yr of age. It is assumed that host factors influence the severity of the disease presentation and thus the ... [more ▼]

Respiratory syncytial virus (RSV) is a prominent cause of airway morbidity in children under 1 yr of age. It is assumed that host factors influence the severity of the disease presentation and thus the need for hospitalization. As a first step toward the identification of the underlying genes involved, this study was undertaken to establish whether inbred mouse strains differ in susceptibility to pneumonia virus of mice (PVM), the murine counterpart of RSV, which has been shown to accurately mimic the RSV disease of children. With this purpose in mind, double-chamber plethysmography and carbon monoxide uptake data were collected daily for 7 days after inoculation of PVM in six inbred strains of mice. In parallel, histological examinations and lung viral titration were carried out from day 5 to day 7 after inoculation. Pulmonary structure/function values reflected the success of viral replication in the lungs and revealed a pattern of continuous variation, with resistant, intermediate, and susceptible strains. The results suggest that SJL (resistant) and 129/Sv (susceptible) strains should be used in crossing experiments aimed at identifying genes controlling pneumovirus replication by the positional cloning approach. Similarly, crossing experiments using BALB/c or C57BL/6 (resistant) and DBA/2 or 129/Sv (susceptible) will allow the identification of the genes involved in the control of pulmonary inflammation during pneumovirus infection. [less ▲]

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See detailKey roles of LFA-1 in leukocyte migration and immune response
Zecchinon, Laurent ULg; Fett, Thomas ULg; Vanden Bergh, Philippe ULg et al

in Clinical & Applied Immunology Reviews (2006), 6

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See detailAnatomy of the Lymphocyte function-associated antigen-1
Zecchinon, Laurent ULg; Fett, Thomas ULg; Vanden Bergh, Philippe ULg et al

in Clinical & Applied Immunology Reviews (2006), 6

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See detailGenomic structure, promoter analysis, and expression of the porcine (Sus scrofa) Mx1 gene
Thomas, Anne; Palm, Mélanie; Broers, Aurore ULg et al

in Immunogenetics (2006), 58

Allelic polymorphisms at the mouse Mx1 locus affect the probability of survival after experimental influenzal disease, raising the possibility that marker-assisted selection using the homologous locus ... [more ▼]

Allelic polymorphisms at the mouse Mx1 locus affect the probability of survival after experimental influenzal disease, raising the possibility that marker-assisted selection using the homologous locus could improve the innate resistance of pigs to natural influenza infections. Several issues need to be resolved before efficient large scale screening of the allelic polymorphism at the porcine (Sus scrofa) Mx1 locus can be implemented. First, the Mx1 genomic structure has to be established and sufficient flanking intronic sequences have to be gathered to enable simple PCR amplification of the coding portions of the gene. Then, a basic knowledge of the promoter region needs to be obtained as an allelic variation there can significantly alter absolute levels and/or tissue-specificity of MX protein expression. The results gathered here show that the porcine Mx1 gene and promoter share the major structural and functional characteristics displayed by their homologs described in cattle, mouse, chicken, and man. The crucial function of the proximal interferon-sensitive response elements motif for gene expression is also demonstrated. The sequence data compiled here will allow an extensive analysis of the polymorphisms present among the widest spectrum possible of porcine breeds with the aim to identify an Mx1 allele providing antiviral resistance. [less ▲]

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See detailLFA-1 and associated diseases: the dark side of a receptor
Zecchinon, Laurent ULg; Fett, Thomas ULg; Vanden Bergh, Philippe ULg et al

in Clinical & Applied Immunology Reviews (2006), 6

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See detailBind another day: the LFA-1/ICAM-1 interation as therapeutic target
Zecchinon, Laurent ULg; Fett, Thomas ULg; Vanden Bergh, Philippe ULg et al

in Clinical & Applied Immunology Reviews (2006), 6

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See detailSuppression of pattern recognition receptor TLR4 sensing does not alter lung responses to pneumovirus infection
Faisca, Pedro; Bui Tran Anh, Dao; Thomas, Anne et al

in Microbes & Infection (2006), 8

Toll-like receptors (TLR) are an important component in the innate immune response to a wide variety of pathogens. Recently, a series of studies has addressed the hypothesis that TLR4 also participates in ... [more ▼]

Toll-like receptors (TLR) are an important component in the innate immune response to a wide variety of pathogens. Recently, a series of studies has addressed the hypothesis that TLR4 also participates in the host innate response against respiratory syncytial virus (RSV), the leading cause of lower respiratory tract infections in infants and young children. In most of the studies available, RSV, which is not a natural pathogen of mice, has been systematically used in mouse models of human bronchiolitis, with conflicting results. Pneumonia virus of mice (PVM), a member of the pneumovirus genus, shares many similarities with RSV. The serological and structural relationships that exist between them suggest that the immune response to these viruses may be similar in their respective natural hosts. To determine the role of TLR4 in host defense against PVM, TLR4-competent and TLR4-deficient mice were intranasally infected with PVM. Variation of body weight, pulmonary function values, histopathology, and pulmonary viral loads were analyzed. None of the investigated clinical, functional, histological and virological parameters was different between strains, which demonstrates that the sensitivity of the mouse to its natural pneumovirus infection is independent of the presence or absence of TLR4 sensing. [less ▲]

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See detailFlow Cytometry for the follow-up of Trypanosoma evansi infection in a mouse model
Antoine-Moussiaux, Nicolas ULg; Leroy, ; Faisca, et al

Poster (2005, November 18)

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See detailFirst results of chronic wasting disease (CWD) surveillance in the south-eastern part of Belgium
Roels, Stefan; De Bosschere, H.; Saegerman, Claude ULg et al

in Veterinary Quarterly (The) (2005), 27(3), 98-104

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See detailProanthocyanidins, from Ribes Nigrum Leaves, Reduce Endothelial Adhesion Molecules Icam-1 and Vcam-1
Garbacki, Nancy ULg; Kinet, Marie; Nusgens, Betty ULg et al

in Journal of Inflammation (London, England) (2005), 2

BACKGROUND: The effects of proanthocyanidins (PACs), isolated from blackcurrant (Ribes nigrum L.) leaves, on neutrophil accumulation during inflammatory processes were investigated in vivo and in vitro ... [more ▼]

BACKGROUND: The effects of proanthocyanidins (PACs), isolated from blackcurrant (Ribes nigrum L.) leaves, on neutrophil accumulation during inflammatory processes were investigated in vivo and in vitro. METHODS: In vivo studies were performed using carrageenin-induced pleurisy in rats pre-treated with PACs. Exudate volume and PMNs accumulation were measured. Leukocyte cell adhesion molecules (LFA-1, Mac-1 and VLA-4) mobilization in circulating granulocytes were analysed by flow cytometry and endothelial cell adhesion molecules (ICAM-1 and VCAM-1) were detected by immunohistochemistry on lung sections. In vitro studies were conducted on endothelial LT2 cells, stimulated with TNF-alpha, to evaluate ICAM-1, IL-8 and VEGF mRNA expression upon PACs treatment. Data sets were examined by one-way analysis of variance (ANOVA) followed by a Scheffe post-hoc test. RESULTS: Pretreatment of the animals with PACs (10, 30 and 60 mg/kg) inhibited dose-dependently carrageenin-induced pleurisy in rats by reducing pleural exudate formation and PMNs infliltration. Leukocyte cell adhesion molecules mobilization was not down-regulated on granulocytes by PACs. Immunohistochemistry on lung sections showed a decreased production of endothelial cell adhesion molecules. In vitro experiments demonstrated that PACs were able to significantly inhibit ICAM-1 but not IL-8 and VEGF165 mRNA expression. Moreover, VEGF121 mRNA expression was dose-dependently enhanced. CONCLUSION: This study provides evidence to support the anti-inflammatory activity of proanthocyanidins is related to an inhibition of leukocyte infiltration which can be explained at least in part by a down-regulation of endothelial adhesion molecules, ICAM-1 and VCAM-1 and that these compounds are capable of modulating TNF-alpha-induced VEGF transcription. [less ▲]

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See detailEpidemiology of atypical myopathy: descriptive phase
Votion, Dominique ULg; Linden, Annick ULg; Desmecht, Daniel ULg et al

in In Proceeding: First Scientific Symposium of the European College of Equine Internal Medicine (ECEIM) (2005, January)

Atypical myopathy (AM) in grazing horses is a frequently fatal condition that has been reported in several European countries. In Belgium, since autumn 2000, AM was confirmed in 39 equids based on ... [more ▼]

Atypical myopathy (AM) in grazing horses is a frequently fatal condition that has been reported in several European countries. In Belgium, since autumn 2000, AM was confirmed in 39 equids based on characteristic histological lesions in postural and respiratory muscles. This study describes the history, clinical signs and biochemical changes associated with AM in these confirmed cases. [less ▲]

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See detailResistance of paramyxoviridae to type I interferon-induced Bos taurus Mx1 dynamin
Leroy, Michael; Baise, Etienne ULg; Pire, Grégory et al

in Journal of Interferon & Cytokine Research (2005), 25(4), 192-201

Typical targets of type I interferon (IFN)-induced antiviral Mx proteins known to date have been shown to share a common profile: single-stranded negative-sense RNA viruses. Among them, human MxA is known ... [more ▼]

Typical targets of type I interferon (IFN)-induced antiviral Mx proteins known to date have been shown to share a common profile: single-stranded negative-sense RNA viruses. Among them, human MxA is known to interfere with the replication of measles, human, and bovine parainfluenza-3 viruses (BoPi3V), that is, three members of the Paramyxoviridae family. Recently, bovine Mx1 protein (BoMx1) was included in the group of Mx proteins with authenticated antiviral potential, as it dramatically represses the replication of vesicular stomatitis virus (VSV). As replication in bovine cells of Pi3, respiratory syncytial (RS), and Sendai (Se) viruses, all members of the same family, is known to be reduced on IFN-alpha incorporation into the culture medium, it was hypothesized that the BoMx1 pathway possibly was involved, its antiviral spectrum thus probably extending to Paramyxoviridae. In this study, probing of BoMx1-inhibiting effects was carried out by infecting a transgenic Vero cell line that allows tightly regulated conditional expression of BoMx1 after doxycycline treatment with a wide array of Paramyxoviridae. Expressing and nonexpressing cells displayed similar viability, cytopathic effects (CPEs), and amounts of infectious virus yields, whatever the infecting virus or the multiplicity of infection (moi) imposed. It is, therefore, concluded that BoMx1 does not interfere with Paramyxoviridae. [less ▲]

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See detailCloning and characterisation of the primary structure of the sheep lymphocyte function-associated antigen-1 alpha subunit
Fett, Thomas ULg; Zecchinon, Laurent ULg; Baise, Etienne ULg et al

in Molecular Immunology (2005), 42(12), 1503-1508

The leukocyte integrins play a critical role in a number of cellular adhesive interactions during the immune response. The ovine cDNA encoding CD1 1a, the predominant a subunit of the beta(2)-integrin ... [more ▼]

The leukocyte integrins play a critical role in a number of cellular adhesive interactions during the immune response. The ovine cDNA encoding CD1 1a, the predominant a subunit of the beta(2)-integrin family, was sequenced and compared with the human, bovine and murine sequences. Despite some focal differences, it shares all the main characteristics of its known mammalian homologues. Along with the ovine CD18-encoding cDNA, which is available for a few months, the sequence data provided here will allow the Ovis aries beta(2)-integrin CD1 1a/CD18 (LEA-1, alpha(L)beta 2) expression in vitro as a tool to examine the specificities of inflammation in the ovine species. (c) 2005 Elsevier Ltd. All rights reserved. [less ▲]

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See detailHow Mannheimia haemolytica defeats host defence through a kiss of death mechanism
Zecchinon, Laurent ULg; Fett, Thomas ULg; Desmecht, Daniel ULg

in Veterinary Research (2005), 36(Mar-Apr), 133-156

Mannheimia haemolytica induced pneumonias are only observed in goats, sheep and cattle. The bacterium produces several virulence factors,whose principal ones are lipopolysaccharide and leukotoxin. The ... [more ▼]

Mannheimia haemolytica induced pneumonias are only observed in goats, sheep and cattle. The bacterium produces several virulence factors,whose principal ones are lipopolysaccharide and leukotoxin. The latter is cytotoxic only for ruminant leukocytes, a phenomenon that is correlated with its ability to bind and interact with the ruminant beta2-integrin Lymphocyte Function-associated Antigen 1. This paper globally reviews all the information available on host-pathogen interactions underlying respiratory mannheimiosis (formerly pasteurellosis), from the stable and the Petri dish to the biochemical cascade of events triggered by the leukotoxin inside ruminant leukocytes. One conclusion can be made: the most widespread cattle respiratory disease with the most important impact on beef production worldwide, is probably due to a tiny ruminant-specific focal variation in the CD18- and/or CD11a-expressing genes. [less ▲]

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See detailSendai virus-induced alterations in lung structure/function correlate with viral loads and reveal a wide resistance/susceptibility spectrum among mouse strains
Faisca, Pedro; Bui Tran Anh, Dao; Desmecht, Daniel ULg

in American Journal of Physiology - Lung Cellular and Molecular Physiology (2005), 289

The Paramyxoviridae family includes some of the most important and ubiquitous disease-causing viruses of infants and children, most of which cause significant infections of the respiratory tract. Evidence ... [more ▼]

The Paramyxoviridae family includes some of the most important and ubiquitous disease-causing viruses of infants and children, most of which cause significant infections of the respiratory tract. Evidence is accumulating in humans that genetic factors are involved in the severity of clinical presentation. As a first step toward the identification of the genes involved, this study was undertaken to establish whether laboratory mouse strains differ in susceptibility to Sendai virus, the murine counterpart of human type-1 parainfluenza virus which, historically, has been used extensively in studies that have defined the basic biological properties of paramyxoviruses in general. With this purpose in mind, double-chamber plethysmography data were collected daily for 7 days after inoculation of Sendai virus in six inbred strains of mice. In parallel, histological examinations and lung viral titration were carried out from day 5 to day 7 after inoculation. Pulmonary structure/function values closely reflected the success of viral replication in the lungs and revealed a pattern of continuous variation with resistant, intermediate, and susceptible strains. The results unambiguously suggest that BALB/c (resistant) and 129Sv (susceptible) strains should be used in crossing experiments aimed at identifying the genes involved in resistance to Paramyxoviridae by the positional cloning approach. [less ▲]

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See detailMolecular characterization of the caprine (Capra hircus) lymphocyte function-associated antigen-1 alpha subunit
Fett, Thomas ULg; Zecchinon, Laurent ULg; Baise, Etienne ULg et al

in BMC Veterinary Research (2005)

Background: Lymphocyte function-associated antigen-1 (LFA-1, CD11a/CD18, alpha L beta 2) is required for many cellular adhesive interactions during the immune response. Results: The Capra hircus CD11a ... [more ▼]

Background: Lymphocyte function-associated antigen-1 (LFA-1, CD11a/CD18, alpha L beta 2) is required for many cellular adhesive interactions during the immune response. Results: The Capra hircus CD11a-encoding cDNA was sequenced and compared with its human, murine, rat, bovine and ovine counterparts. Despite some focal differences, it shares all the main characteristics of its known mammalian homologues. Conclusion: Therefore, along with the caprine CD18-encoding cDNA, which has been available for a few months, the sequence data revealed here will allow the Capra hircus LFA-1 expression in vitro as a tool to explore the specificities of inflammation in the caprine species. [less ▲]

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