References of "Desmecht, Daniel"
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See detailFlow Cytometry for the follow-up of Trypanosoma evansi infection in a mouse model
Antoine-Moussiaux, Nicolas ULg; Leroy, ; Faisca, et al

Poster (2005, November 18)

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See detailFirst results of chronic wasting disease (CWD) surveillance in the south-eastern part of Belgium
Roels, Stefan; De Bosschere, H.; Saegerman, Claude ULg et al

in Veterinary Quarterly (The) (2005), 27(3), 98-104

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See detailProanthocyanidins, from Ribes Nigrum Leaves, Reduce Endothelial Adhesion Molecules Icam-1 and Vcam-1
Garbacki, Nancy ULg; Kinet, Marie; Nusgens, Betty ULg et al

in Journal of Inflammation (London, England) (2005), 2

BACKGROUND: The effects of proanthocyanidins (PACs), isolated from blackcurrant (Ribes nigrum L.) leaves, on neutrophil accumulation during inflammatory processes were investigated in vivo and in vitro ... [more ▼]

BACKGROUND: The effects of proanthocyanidins (PACs), isolated from blackcurrant (Ribes nigrum L.) leaves, on neutrophil accumulation during inflammatory processes were investigated in vivo and in vitro. METHODS: In vivo studies were performed using carrageenin-induced pleurisy in rats pre-treated with PACs. Exudate volume and PMNs accumulation were measured. Leukocyte cell adhesion molecules (LFA-1, Mac-1 and VLA-4) mobilization in circulating granulocytes were analysed by flow cytometry and endothelial cell adhesion molecules (ICAM-1 and VCAM-1) were detected by immunohistochemistry on lung sections. In vitro studies were conducted on endothelial LT2 cells, stimulated with TNF-alpha, to evaluate ICAM-1, IL-8 and VEGF mRNA expression upon PACs treatment. Data sets were examined by one-way analysis of variance (ANOVA) followed by a Scheffe post-hoc test. RESULTS: Pretreatment of the animals with PACs (10, 30 and 60 mg/kg) inhibited dose-dependently carrageenin-induced pleurisy in rats by reducing pleural exudate formation and PMNs infliltration. Leukocyte cell adhesion molecules mobilization was not down-regulated on granulocytes by PACs. Immunohistochemistry on lung sections showed a decreased production of endothelial cell adhesion molecules. In vitro experiments demonstrated that PACs were able to significantly inhibit ICAM-1 but not IL-8 and VEGF165 mRNA expression. Moreover, VEGF121 mRNA expression was dose-dependently enhanced. CONCLUSION: This study provides evidence to support the anti-inflammatory activity of proanthocyanidins is related to an inhibition of leukocyte infiltration which can be explained at least in part by a down-regulation of endothelial adhesion molecules, ICAM-1 and VCAM-1 and that these compounds are capable of modulating TNF-alpha-induced VEGF transcription. [less ▲]

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See detailEpidemiology of atypical myopathy: descriptive phase
Votion, Dominique ULg; Linden, Annick ULg; Desmecht, Daniel ULg et al

in In Proceeding: First Scientific Symposium of the European College of Equine Internal Medicine (ECEIM) (2005, January)

Atypical myopathy (AM) in grazing horses is a frequently fatal condition that has been reported in several European countries. In Belgium, since autumn 2000, AM was confirmed in 39 equids based on ... [more ▼]

Atypical myopathy (AM) in grazing horses is a frequently fatal condition that has been reported in several European countries. In Belgium, since autumn 2000, AM was confirmed in 39 equids based on characteristic histological lesions in postural and respiratory muscles. This study describes the history, clinical signs and biochemical changes associated with AM in these confirmed cases. [less ▲]

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See detailResistance of paramyxoviridae to type I interferon-induced Bos taurus Mx1 dynamin
Leroy, Michael; Baise, Etienne ULg; Pire, Grégory et al

in Journal of Interferon & Cytokine Research (2005), 25(4), 192-201

Typical targets of type I interferon (IFN)-induced antiviral Mx proteins known to date have been shown to share a common profile: single-stranded negative-sense RNA viruses. Among them, human MxA is known ... [more ▼]

Typical targets of type I interferon (IFN)-induced antiviral Mx proteins known to date have been shown to share a common profile: single-stranded negative-sense RNA viruses. Among them, human MxA is known to interfere with the replication of measles, human, and bovine parainfluenza-3 viruses (BoPi3V), that is, three members of the Paramyxoviridae family. Recently, bovine Mx1 protein (BoMx1) was included in the group of Mx proteins with authenticated antiviral potential, as it dramatically represses the replication of vesicular stomatitis virus (VSV). As replication in bovine cells of Pi3, respiratory syncytial (RS), and Sendai (Se) viruses, all members of the same family, is known to be reduced on IFN-alpha incorporation into the culture medium, it was hypothesized that the BoMx1 pathway possibly was involved, its antiviral spectrum thus probably extending to Paramyxoviridae. In this study, probing of BoMx1-inhibiting effects was carried out by infecting a transgenic Vero cell line that allows tightly regulated conditional expression of BoMx1 after doxycycline treatment with a wide array of Paramyxoviridae. Expressing and nonexpressing cells displayed similar viability, cytopathic effects (CPEs), and amounts of infectious virus yields, whatever the infecting virus or the multiplicity of infection (moi) imposed. It is, therefore, concluded that BoMx1 does not interfere with Paramyxoviridae. [less ▲]

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See detailCloning and characterisation of the primary structure of the sheep lymphocyte function-associated antigen-1 alpha subunit
Fett, Thomas ULg; Zecchinon, Laurent ULg; Baise, Etienne ULg et al

in Molecular Immunology (2005), 42(12), 1503-1508

The leukocyte integrins play a critical role in a number of cellular adhesive interactions during the immune response. The ovine cDNA encoding CD1 1a, the predominant a subunit of the beta(2)-integrin ... [more ▼]

The leukocyte integrins play a critical role in a number of cellular adhesive interactions during the immune response. The ovine cDNA encoding CD1 1a, the predominant a subunit of the beta(2)-integrin family, was sequenced and compared with the human, bovine and murine sequences. Despite some focal differences, it shares all the main characteristics of its known mammalian homologues. Along with the ovine CD18-encoding cDNA, which is available for a few months, the sequence data provided here will allow the Ovis aries beta(2)-integrin CD1 1a/CD18 (LEA-1, alpha(L)beta 2) expression in vitro as a tool to examine the specificities of inflammation in the ovine species. (c) 2005 Elsevier Ltd. All rights reserved. [less ▲]

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See detailHow Mannheimia haemolytica defeats host defence through a kiss of death mechanism
Zecchinon, Laurent ULg; Fett, Thomas ULg; Desmecht, Daniel ULg

in Veterinary Research (2005), 36(Mar-Apr), 133-156

Mannheimia haemolytica induced pneumonias are only observed in goats, sheep and cattle. The bacterium produces several virulence factors,whose principal ones are lipopolysaccharide and leukotoxin. The ... [more ▼]

Mannheimia haemolytica induced pneumonias are only observed in goats, sheep and cattle. The bacterium produces several virulence factors,whose principal ones are lipopolysaccharide and leukotoxin. The latter is cytotoxic only for ruminant leukocytes, a phenomenon that is correlated with its ability to bind and interact with the ruminant beta2-integrin Lymphocyte Function-associated Antigen 1. This paper globally reviews all the information available on host-pathogen interactions underlying respiratory mannheimiosis (formerly pasteurellosis), from the stable and the Petri dish to the biochemical cascade of events triggered by the leukotoxin inside ruminant leukocytes. One conclusion can be made: the most widespread cattle respiratory disease with the most important impact on beef production worldwide, is probably due to a tiny ruminant-specific focal variation in the CD18- and/or CD11a-expressing genes. [less ▲]

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See detailSendai virus-induced alterations in lung structure/function correlate with viral loads and reveal a wide resistance/susceptibility spectrum among mouse strains
Faisca, Pedro; Bui Tran Anh, Dao; Desmecht, Daniel ULg

in American Journal of Physiology - Lung Cellular and Molecular Physiology (2005), 289

The Paramyxoviridae family includes some of the most important and ubiquitous disease-causing viruses of infants and children, most of which cause significant infections of the respiratory tract. Evidence ... [more ▼]

The Paramyxoviridae family includes some of the most important and ubiquitous disease-causing viruses of infants and children, most of which cause significant infections of the respiratory tract. Evidence is accumulating in humans that genetic factors are involved in the severity of clinical presentation. As a first step toward the identification of the genes involved, this study was undertaken to establish whether laboratory mouse strains differ in susceptibility to Sendai virus, the murine counterpart of human type-1 parainfluenza virus which, historically, has been used extensively in studies that have defined the basic biological properties of paramyxoviruses in general. With this purpose in mind, double-chamber plethysmography data were collected daily for 7 days after inoculation of Sendai virus in six inbred strains of mice. In parallel, histological examinations and lung viral titration were carried out from day 5 to day 7 after inoculation. Pulmonary structure/function values closely reflected the success of viral replication in the lungs and revealed a pattern of continuous variation with resistant, intermediate, and susceptible strains. The results unambiguously suggest that BALB/c (resistant) and 129Sv (susceptible) strains should be used in crossing experiments aimed at identifying the genes involved in resistance to Paramyxoviridae by the positional cloning approach. [less ▲]

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See detailMolecular characterization of the caprine (Capra hircus) lymphocyte function-associated antigen-1 alpha subunit
Fett, Thomas ULg; Zecchinon, Laurent ULg; Baise, Etienne ULg et al

in BMC Veterinary Research (2005)

Background: Lymphocyte function-associated antigen-1 (LFA-1, CD11a/CD18, alpha L beta 2) is required for many cellular adhesive interactions during the immune response. Results: The Capra hircus CD11a ... [more ▼]

Background: Lymphocyte function-associated antigen-1 (LFA-1, CD11a/CD18, alpha L beta 2) is required for many cellular adhesive interactions during the immune response. Results: The Capra hircus CD11a-encoding cDNA was sequenced and compared with its human, murine, rat, bovine and ovine counterparts. Despite some focal differences, it shares all the main characteristics of its known mammalian homologues. Conclusion: Therefore, along with the caprine CD18-encoding cDNA, which has been available for a few months, the sequence data revealed here will allow the Capra hircus LFA-1 expression in vitro as a tool to explore the specificities of inflammation in the caprine species. [less ▲]

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See detailTreatment of experimental asthma by decoy-mediated local inhibition of activator protein-1
Desmet, Christophe ULg; Gosset, P.; Henry, E. et al

in American Journal of Respiratory & Critical Care Medicine (2005), 172(6), 671-678

Rationale: Asthma is associated with increased expression of a typical array of genes involved in immune and inflammatory responses, including those encoding the prototypic Th2 cytokines interleukin (IL ... [more ▼]

Rationale: Asthma is associated with increased expression of a typical array of genes involved in immune and inflammatory responses, including those encoding the prototypic Th2 cytokines interleukin (IL) 4, IL-5, and IL-13. Most of these genes contain binding sites for activator protein-1 (AP-1) within their promoter and are therefore believed to depend on AP-1 for their expression, suggesting that this transcription factor could be of particular importance in asthma pathophysiology. Objective: To clarify the role of AP-1 in the effector phase of pulmonary allergy. Methods: Ovalbumin (OVA)-sensitized mice were intratracheally given decoy oligodeoxyribonucleotides (ODNs) specifically directed to AP-1 or scrambled control ODNs before challenge with aerosolized OVA. Twenty-four hours after the last OVA challenge, airway hyperresponsiveness was measured and allergic airway inflammation was evaluated quantitatively. AP-1 decoys were localized using flow cytometry and immunohistochemistry. AP-1 activity in the lung was assessed using electrophoretic mobility shift assay. Measurements and Main Results: Intratracheally delivered AP-1 decoys efficiently targeted airway immune cells, thus precluding AP-1 activation on OVA challenge. Decoy-mediated local inhibition of AP-1 resulted in significant attenuation of all the pathophysiologic features of experimental asthma-namely, eosinophilic airway inflammation, airway hyperresponsiveness, mucous cell hyperplasia, production of allergen-specific immunoglobulins, and synthesis of IL-4, IL-5, and IL-13. Scrambled control ODNs had no detectable effects. Conclusions: Our results reveal a key role for AP-1 in the effector phase of pulmonary allergy and indicate that specific AP-1 inhibition in the airways may have therapeutic value in the control of established asthma. [less ▲]

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See detailClues for Differential Diagnosis of atypical myopathy
Votion, Dominique ULg; Amory, Hélène ULg; Sandersen, Charlotte ULg et al

in Proceeding of the Maastricht International Congress on Equine Medicine (MICEM) (2004, December)

Atypical myopathy (AM), also called “atypical myoglobinuria”, is a frequently and rapidly fatal myopathy of unknown origin occurring sporadically in grazing horses. As opposed to the exertional ... [more ▼]

Atypical myopathy (AM), also called “atypical myoglobinuria”, is a frequently and rapidly fatal myopathy of unknown origin occurring sporadically in grazing horses. As opposed to the exertional rhabdomyolysis syndrome (ERS), clinical signs of AM are not induced by exercise. The condition has been reported in several European countries including Belgium, France, Germany and Great Britain. Clinical signs of AM are characterised by muscular weakness, stiffness, recumbency, sweating and when urine is observed, myoglobinuria. These signs are characteristic but not pathognomonic of the disease; the differential diagnosis of sudden weakness, severe myopathy and/or unexpected death includes several neurogenic and myopathic disorders. The main pathologies that share clinical similarities with AM include the acute form of grass sickness (GS), acute piroplasmosis, botulism, ERS, the hyperkalemic periodic paresis (HPP), nutritional myopathy (NM; i.e. vitamin E and/or selenium deficiency), plants or drugs (i.e. ionophores intoxication) intoxication, tetanus and postanaesthesia myopathy. This report aims at reviewing key facts in history, clinical signs, clinical examination and laboratory findings that contribute towards the diagnosis of AM and/or invalidate the diagnosis of other pathologies. [less ▲]

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See detailErgot poisoning in cattle
Brihoum, M.; Desmecht, Daniel ULg; Bony, S. et al

in Annales de Médecine Vétérinaire (2004), 148(2), 97-101

In addition to the financial losses that ergot ( Claviceps purpurea) contamination causes in crops, the ingestion of ergot-contaminated food by cattle results in an intoxication that develops in several ... [more ▼]

In addition to the financial losses that ergot ( Claviceps purpurea) contamination causes in crops, the ingestion of ergot-contaminated food by cattle results in an intoxication that develops in several patterns. The observed clinical troubles may involve different systems. In this paper, we reviewed the clinical signs observed in ergot-poisoned cattle and some diagnostic and prevention aspects. [less ▲]

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See detailBiochemical findings associated with atypical myopathy in grazing horses
Votion, Dominique ULg; Delguste, Catherine ULg; Amory, Hélène ULg et al

in Pflügers Archiv : European Journal of Physiology (2004), 447

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See detailAtypical Myopathy (Atypical Myoglobinuria)
Votion, Dominique ULg; Amory, Hélène ULg; Demoulin, Vincent ULg et al

in IVIS Reviews in Veterinary Medicine (2004)

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See detailMolecular cloning and characterisation of the CD18 partner in ovine (Ovis aries) beta(2)-integrins
Zecchinon, Laurent ULg; Fett, Thomas ULg; Baise, Etienne ULg et al

in Gene (2004), 334

The leukocyte integrins play a critical role in a number of cellular adhesive interactions during the immune response. We describe here the isolation and characterization of the ovine beta(2) (CD18 ... [more ▼]

The leukocyte integrins play a critical role in a number of cellular adhesive interactions during the immune response. We describe here the isolation and characterization of the ovine beta(2) (CD18) subunit, common to the leukocyte beta(2)-integrin family. The deduced 770-amino-acid sequence reveals a transmembrane protein with 81%, 83% and 95% identity with its murine, human and bovine homologues, respectively. Comparisons of CD18 sequences emphasize the functional importance of the beta(2) subunit I-like domain and included metal ion-dependent adhesion site (MIDAS)-like motif and confirm that of the cytoplasmic tail. The data provided here will offer the possibility to explore new avenues in studies based on the ovine model. (C) 2004 Elsevier B.V. All rights reserved. [less ▲]

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See detailThe bovine (Bos taurus) CD11a-encoding cDNA: molecular cloning, characterisation and comparison with the human and murine glycoproteins
Fett, Thomas ULg; Zecchinon, Laurent ULg; Baise, Etienne ULg et al

in Gene (2004), 325

The bovine cDNA encoding CD11 a cell-surface glycoprotein involved in multiple leukocyte functions, was sequenced and compared with the human and murine sequences. Despite some focal differences, it ... [more ▼]

The bovine cDNA encoding CD11 a cell-surface glycoprotein involved in multiple leukocyte functions, was sequenced and compared with the human and murine sequences. Despite some focal differences, it shares all the main characteristics of its known mammalian homologs. Along with the bovine CD18-encoding cDNA, which is available for a long time, the sequence data provided here will allow the successful expression of bovine CD11a, thus giving the first opportunity to express the Bos taurus beta(2)-integrin CD11a/CD18 (LFA-1, alpha(L)beta(2)) in vitro as a tool to examine the specificities of inflammation in the bovine species. (C) 2003 Elsevier B.V. All rights reserved. [less ▲]

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See detailCharacterization of the caprine (Capra hircus) beta-2 integrin CD18-encoding cDNA and identification of mutations potentially responsible for the ruminant-specific virulence of Mannheimia haemolytica
Zecchinon, Laurent ULg; Fett, Thomas ULg; Baise, Etienne ULg et al

in Molecular Membrane Biology (2004), 21(5, Sep-Oct), 289-295

The leukocyte integrins play a critical role in a great number of cellular adhesive interactions during the immune response. We describe here the isolation and characterization of the caprine beta(2 ... [more ▼]

The leukocyte integrins play a critical role in a great number of cellular adhesive interactions during the immune response. We describe here the isolation and characterization of the caprine beta(2) (CD18) sub-unit, common to the leukocyte beta(2)-integrin family. The deduced 770-amino-acid sequence reveals a transmembrane protein with 80, 81, 83, 96 and 99% identity with its canine, murine, human, bovine and ovine homologues respectively. Analysis of CD18 sequences emphasizes the functional importance of the beta(2) sub-unit I-like domain, and included metal ion-dependent adhesion site-like motif and confirms that of the cytoplasmic tail. Moreover, comparisons of ruminant versus non-ruminant CD18 sequences allowed the identification of 16 potential mutation sites that could be held responsible for the unique virulence of Mannheimia haemolytica for ruminants. Mannheimiosis is known to be the major respiratory disease among ruminants, whereas it is not pathogenic for other mammals, an observation that has been attributed to a specific interaction between M. haemolytica leukotoxin and ruminants' CD18. Therefore, the data provided here offer the possibility to explore new avenues in studies based on the caprine model and provide key information for future studies aimed at elucidating the molecular mechanisms underlying the ruminant-specific virulence of M. haemolytica. [less ▲]

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See detailExtensive mast cell degranulation in bovine respiratory syncytial virus-associated paroxystic respiratory distress syndrome
Jolly, Sandra ULg; Detilleux, Johann ULg; Desmecht, Daniel ULg

in Veterinary Immunology and Immunopathology (2004), 97(3-4), 125-136

Bovine respiratory syncytial virus (BRSV) infection is an important cause of outbreaks of respiratory disease among calves. This virus commonly induces mild to severe respiratory signs but, in a ... [more ▼]

Bovine respiratory syncytial virus (BRSV) infection is an important cause of outbreaks of respiratory disease among calves. This virus commonly induces mild to severe respiratory signs but, in a substantial proportion of cases, is also reported to be associated with paroxystic respiratory distress syndrome (PRDS). The pathogenesis of this 'malignant' clinical form has not been fully elucidated. The present study aimed at determining whether mast cell (MC) degranulation plays a role in the physiopathologic cascade leading to the PRDS. Paired serum samples were taken in herds during outbreaks of severe respiratory diseases (acute sera) and 3 weeks after (convalescent sera). Based on seroconversion to BRSV and clinical picture, 67 pairs of sera were selected from calves with a BRSV-associated PRDS for circulating MC tryptase determination. A MC metachromatic score was measured in post-mortem lungs from animals died from a BRSV-associated PRDS (principals) and compared with reference scores obtained from healthy lungs (controls). Levels of tryptase were significantly higher in acute sera (26.6+/-12.4 mug/l) compared to convalescent sera (8.4+/-7.8 mug/l; P < 0.001). Metachromatic scores yielded significantly different results between controls and principals (P < 0.01), demonstrating a significant disappearance of metachromatic granules from lung MCs in principals. Taken together, these data demonstrate the presence of an extensive MC degranulation in BRSV-associated PRDS. (C) 2003 Elsevier B.V. All rights reserved. [less ▲]

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See detailConditional expression of type I interferon-induced bovine Mx1 GTPase in a stable transgenic vero cell line interferes with replication of vesicular stomatitis virus
Baise, Etienne ULg; Pire, Grégory; Leroy, Michael et al

in Journal of Interferon & Cytokine Research (2004), 24(9), 513-521

In some vertebrate species, type I interferon(IFN)-induced Mx gene expression has been shown to confer resistance to some single-stranded RNA (ssRNA) viruses in vitro. Because the bovine species is ... [more ▼]

In some vertebrate species, type I interferon(IFN)-induced Mx gene expression has been shown to confer resistance to some single-stranded RNA (ssRNA) viruses in vitro. Because the bovine species is subject to an exceptionally wide array of infections caused by such viruses, it is anticipated that an antiviral allele should have been retained by evolution at the bovine Mx locus. The identification of such allele may help in evaluating the real significance of the Mx genotype for disease resistance in vivo, in deciphering host-virus molecular interactions involved, or in improving innate disease resistance of livestock through marker-assisted selection. We validated a double transgenic Vero cell clone in which the bovine Mx1 reference allele is placed under control of the human cytomegalovirus (CMV) enhancer-promoter sequence containing elements from the bacterial tetracycline resistance operon to regulate transcription. In the selected clone, transgene repression was very tight, and derepression by doxycycline led to homogeneous 48-h duration expression of physiologic levels of bovine Mx1. Expression of the transgene caused a dramatic decrease in cytopathic efficiency and a 500-5000-fold yield reduction of the Indiana and New Jersey serotypes of vesicular stomatitis virus (VSV). To our knowledge, the transgenic clone developed here is the first ever reported that allows conditional expression of an Mx protein, thus providing a valuable tool for studying functions of Mx proteins in general and that of bovine Mx1 in particular. This latter may henceforward be included in the group of Mx proteins with authenticated anti-VSV activity, which offers new research avenues into the field of host-virus interactions. [less ▲]

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