References of "Denooz, Raphael"
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See detailQuantification in postmortem blood and identification in urine of tramadol and its two main metabolites in two cases of lethal tramadol intoxication
De Backer, Benjamin ULg; Renardy, Françoise ULg; Denooz, Raphaël ULg et al

in Journal of Analytical Toxicology (2010), 34(9), 599-604

Tramadol has been extensively prescribed for two decades. It is an opioid analgesic considered to induce fewer side effects than other compounds of this class. However, serious complications may occur in ... [more ▼]

Tramadol has been extensively prescribed for two decades. It is an opioid analgesic considered to induce fewer side effects than other compounds of this class. However, serious complications may occur in case of intoxication. We report here two cases of fatal intoxication due to tramadol ingestion. Tramadol, O-desmethyltramadol (ODT) and N-desmethyltramadol (NDT) were quantitatively and qualitatively determined in postmortem blood and urine, respectively. An HPLC method coupled with fluorescence detection was validated using total error approach for the analysis of tramadol, ODT, and NDT in blood. In case1, concentrations of tramadol and its metabolites were 7.7 mg/L (tramadol), 1.33 mg/L (ODT), and 0.6mg/L (NDT). In case 2, concentrations found were 48.34 mg/L (tramadol), 2.43 mg/L (ODT), and 10.09 mg/L (NDT). Opposite ratios of ODT/NDT concentrations observed in different cases were suggested to be useful for the evaluation of the delay between ingestion and death. However, the changes in metabolites levels may also be explained by pharmacokinetic interactions and quantitative differences in the activity of the cytochrome-P450 2D6. Interestingly, norfluoxetine was detected in sub-therapeutic levels in case 2. Most of these aspects in tramadol-related fatalities are reviewed in this paper, and an overview of fatal intoxications due to tramadol is presented. [less ▲]

Detailed reference viewed: 73 (12 ULg)
See detailInterest of routine dosage of meropenem in difficult to treat infections
Frippiat, Frédéric ULg; Bensahi, Ilham; Denooz, Raphael ULg et al

Poster (2010, October 23)

Detailed reference viewed: 57 (11 ULg)
See detailScreening médicamenteux et stratégies analytiques en Toxicologie d'urgence : état des lieux
Denooz, Raphael ULg

Scientific conference (2010, October)

Detailed reference viewed: 36 (3 ULg)
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See detailTOLERANCE INTERVALS AS CONTROL CHART: COMPARISON TO CLASSIC SHEWHART X̅-R CONTROL CHART
Marini Djang'Eing'A, Roland ULg; Lambert, Véronique; Denooz, Raphael ULg et al

Poster (2010, September)

Detailed reference viewed: 144 (43 ULg)
See detailIntérêt clinique et économique du suivi thérapeutique pharmacologique pour des médicaments habituellement non contrôlés
Denooz, Raphael ULg

Doctoral thesis (2010)

L’objectif principal de ce travail est de démontrer l’intérêt du suivi thérapeutique pharmacologique appliqué à quelques médicaments ou classes pharmaceutiques habituellement non contrôlés. Le suivi ... [more ▼]

L’objectif principal de ce travail est de démontrer l’intérêt du suivi thérapeutique pharmacologique appliqué à quelques médicaments ou classes pharmaceutiques habituellement non contrôlés. Le suivi thérapeutique pharmacologique repose sur la mesure de la concentration sanguine d’un médicament administré à un patient dans le but d’adapter individuellement la posologie. Les deux principales justifications habituellement retenues pour le suivi thérapeutique pharmacologique sont d’une part la diminution du taux d’échecs thérapeutiques, liés à une mauvaise observance ou à l’emploi d’une posologie insuffisante, et d’autre part la réduction de la fréquence des manifestations indésirables et/ou toxiques des médicaments, plutôt associés à une posologie excessive. La liste des médicaments faisant en pratique l’objet de cette démarche est cependant restreinte à une quarantaine de substances, en raison principalement des différents critères qui justifient l’obtention d’une intervention des soins de santé, à savoir : o l’existence d’une corrélation entre la concentration plasmatique et les effets pharmacodynamiques ; o une zone thérapeutique étroite ; o une variabilité pharmacocinétique interindividuelle importante ; o l’absence d’autres marqueurs permettant d’évaluer l’effet thérapeutique. Le but de ce travail est de convaincre les Cliniciens des avantages d’un élargissement de cette liste conventionnelle à d’autres molécules, en leur présentant une multitude d’autres indications pour le suivi thérapeutique pharmacologique, comme la réduction du coût des thérapies antibactériennes par β-lactamines, l’aide au diagnostic d’une résistance ou d’une hypersensibilité aux anticoagulants oraux, l’évaluation des thérapies substitutives par la méthadone, la vérification de la compliance au traitement par sulfonylurées, ou encore la réduction de la toxicité des traitements à base de bupropion et de GHB. Pour répondre à cet objectif, il était nécessaire de développer et de valider plusieurs techniques qui sont en parfaite adéquation avec les besoins du suivi thérapeutique pharmacologique. [less ▲]

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See detailUPLC MS/MS method for the determination of bupropion and its main metabolites in human whole blood
Denooz, Raphael ULg; Mercerolle, M.; Lachatre, C. et al

in Journal of Analytical Toxicology (2010), 34(5), 280-286

A selective and sensitive ultra-performance liquid chromatography (UPLC)–electrospray ionization-tandem mass spectrometry (MS) method for simultaneous determination of bupropion and its main metabolites ... [more ▼]

A selective and sensitive ultra-performance liquid chromatography (UPLC)–electrospray ionization-tandem mass spectrometry (MS) method for simultaneous determination of bupropion and its main metabolites, hydroxybupropion, erythrohydrobupropion, and threohydrobupropion, in human whole blood is presented. The sample preparation consists of cleanup protein precipitation with methanol combined with a solid-phase extraction on Oasis HLB cartridges. Analytes were separated on a Waters Acquity UPLC® BEH phenyl column using a binary mobile phase consisting of ammonium formate buffer (2 mM, pH 4) and acetonitrile. Detection was performed on a Waters Acquity UPLC system coupled to a Quattro Premier triple-quadrupole MS in positive ion selected reaction monitoring. Internal standards were bupropion-d9 and hydroxybupropion-d6. Linearity was from 5 to 1000 ng/mL for bupropion and from 10 to 2000 ng/mL for metabolites. Accuracy profiles (80–120%), precision (< 15%), and limits of detection (1 ng/mL for bupropion and 2 ng/mL for metabolites) were also evaluated and responded to all criteria of validation. The aim of this study was to compare this presented method with a previously described method developed on a classic liquid chromatography–tandem MS system. [less ▲]

Detailed reference viewed: 107 (14 ULg)
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See detailIntérêt du dosage des sulfamidés hypoglycémiants: de la vérification de la compliance au diagnostic différentiel des hyperinsulinismes
Denooz, Raphael ULg; Charlier, Corinne ULg

in Revue Médicale de Liège (2010), 65(9), 493-497

Plus de cinquante ans après leur mise sur le marché, les sulfamidés hypoglycémiants font toujours partie du traitement de base du diabète non insulinodépendant. A côté de leurs remarquables propriétés ... [more ▼]

Plus de cinquante ans après leur mise sur le marché, les sulfamidés hypoglycémiants font toujours partie du traitement de base du diabète non insulinodépendant. A côté de leurs remarquables propriétés pharmacologiques, ces produits ont, à différentes reprises, été tenus responsables d’hypoglycémies sévères survenant de manière inexpliquée chez des sujets non diabétiques. Ce type d’hypoglycémie, qualifiée de factice, est notamment fréquemment retrouvée dans le syndrome de Munchausen, trouble psychiatrique poussant le malade à mimer une pathologie. Cette hypoglycémie est associée à un hyperinsulinisme endogène simulant une pathologie endocrine très rare, l’insulinome. Nous souhaitons faire connaître aux Cliniciens l’intérêt de la recherche et du dosage sanguin des sulfamidés hypoglycémiants dans le cadre du diagnostic différentiel des hyperinsulinismes. La mise en évidence d’une hypoglycémie induite par ces médicaments constitue un élément intéressant pour orienter le diagnostic et éventuellement éviter une pancréatectomie en cas de forte suspicion d’insulinome. La méthode analytique développée au laboratoire s’appuie sur la chromatographie liquide ultra performante couplée à la spectrométrie de masse tandem (UPLC -MS -MS ). Par cette technique, on peut mettre en évidence dans le sang une dizaine de sulfonylurées et le répaglinide. Elle constituera à l’avenir une méthode de choix face à toute suspicion de mésusage des sulfonylurées. [less ▲]

Detailed reference viewed: 77 (16 ULg)
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See detailQuantification in postmortem blood and identification in urine of tramadol and its two main metabolites in two cases of lethal tramadol intoxication
De Backer, Benjamin ULg; Renardy, Françoise ULg; Denooz, Raphael ULg et al

in Mitteilungsblatt der Gesellschaft für Toxikologische und Forensische Chemie (2010), 77(3), 252

Detailed reference viewed: 61 (5 ULg)
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See detailFatal intoxications by acenocoumarol, phenprocoumon and warfarin: method validation in blood using the total error approach.
Denooz, Raphael ULg; Douamba, Zoénabo; Charlier, Corinne ULg

in Journal of Chromatography. B : Analytical Technologies in the Biomedical & Life Sciences (2009), 877(23), 2344-2348

Detailed reference viewed: 44 (17 ULg)
See detailGestion du contrôle de qualité interne
Denooz, Raphael ULg; Pirson, Laurence ULg

Conference (2009, February)

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See detailIntérêt du dosage des sulfamidés hypoglycémiants
DENOOZ, Raphael ULg

Scientific conference (2009, January)

Detailed reference viewed: 12 (6 ULg)
See detailScreenings Toxicologiques: Apport de l'immunoanalyse
Denooz, Raphael ULg

Conference (2008, June)

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See detailSimultaneous Determination of Five Beta-Lactam Antibiotics (Cefepim, Ceftazidim, Cefuroxim, Meropenem and Piperacillin) in Human Plasma by High-Performance Liquid Chromatography with Ultraviolet Detection
Denooz, Raphael ULg; Charlier, Corinne ULg

in Journal of Chromatography. B : Analytical Technologies in the Biomedical & Life Sciences (2008), 864(1-2), 161-7

Monitoring of plasma antibiotic drugs is useful for better clinical management in infected patients, particularly in intensive care units. A simple and sensitive high-performance liquid chromatography ... [more ▼]

Monitoring of plasma antibiotic drugs is useful for better clinical management in infected patients, particularly in intensive care units. A simple and sensitive high-performance liquid chromatography (HPLC) method with ultraviolet (UV) detection has been developed and validated for simultaneous quantification of five beta-lactam antibiotics in human plasma: cefepim, ceftazidim, cefuroxim, meropenem and piperacillin. The plasma sample, after spiked with ceforanid as an internal standard (IS), was submitted to a solid-phase extraction (SPE) prior to HPLC analysis. A chromatographic separation was achieved on a C8 symmetry column with a mobile phase consisting of an acetonitrile and phosphate buffer (pH 7.4) mixture in a gradient mode. Detection was carried out at a wavelength between 200 and 400nm. The method developed was linear over the concentration range of 2.5-60mug/mL for each antibiotic in the plasma samples. Accuracy ranged from 93.2 to 107.1% and precision was between 0.9 and 12.2%. The method has been applied to plasma samples obtained from patients treated with beta-lactam antibiotics and is appropriated for easy determination of plasma concentrations for therapeutic monitoring applications. [less ▲]

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See detailMechanisms of Interaction of Endocrine-Disrupting Chemicals with Glutamate-Evoked Secretion of Gonadotropin-Releasing Hormone
Rasier, Gregory; Parent, Anne-Simone ULg; Gerard, Arlette ULg et al

in Toxicological Sciences (2008), 102(1), 33-41

In previous studies, we detected a dichlorodiphenyltrichloroethane (DDT) derivative in the serum of children with sexual precocity after migration from developing countries. Recently, we reported that DDT ... [more ▼]

In previous studies, we detected a dichlorodiphenyltrichloroethane (DDT) derivative in the serum of children with sexual precocity after migration from developing countries. Recently, we reported that DDT stimulated pulsatile gonadotropin-releasing hormone (GnRH) secretion and sexual maturation in the female rat. The aim of this study was to delineate the mechanisms of interaction of endocrine-disrupting chemicals including DDT with GnRH secretion evoked by glutamate in vitro. Using hypothalamic explants obtained from 15-day-old female rats, estradiol (E2) and DDT caused a concentration-related increase in glutamate-evoked GnRH release while p,p'-dichlorodiphenyldichloroethene and methoxychlor had no effect. The effective DDT concentrations in vitro were consistent with the serum concentrations measured in vivo 5 days after exposure of immature rats to 10 mg/kg/day of o,p'-DDT. Bisphenol A induced some stimulatory effect, whereas no change was observed with 4-nonylphenol. The o,p'-DDT effects in vitro were prevented partially by a selective antagonist of the alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) subtype of glutamate receptors. A complete prevention of o,p'-DDT effects was caused by an estrogen receptor (ER) antagonist as well as an aryl hydrocarbon receptor (AHR) antagonist and inhibitors of protein kinases A and C and mitogen-activated kinases. While an intermittent incubation with E2 caused no change in amplification of the glutamate-evoked GnRH release for 4 h, continuous incubation with E2 or o,p'-DDT caused an increase of this amplification after 3.5 h of incubation. In summary, DDT amplifies the glutamate-evoked GnRH secretion in vitro through rapid and slow effects involving ER, AHR, and AMPA receptor mediation. [less ▲]

Detailed reference viewed: 37 (10 ULg)
See detailScreenings Toxicologiques: apport d'un système HPLC/DAD
Denooz, Raphael ULg; Dubois, Nathalie ULg

Conference (2008, March)

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See detailSkin protection creams in medical settings: successful or evil ?
Xhauflaire, Emmanuelle ULg; Macarenko, Elena; Denooz, Raphael ULg et al

in Journal of Occupational Medicine and Toxicology (London, England) (2008), 3

ABSTRACT: BACKGROUND: Chronic exposure to mild irritants including cleansing and antiseptic products used for hand hygiene generates insults to the skin. To avoid unpleasant reactions, skin protection ... [more ▼]

ABSTRACT: BACKGROUND: Chronic exposure to mild irritants including cleansing and antiseptic products used for hand hygiene generates insults to the skin. To avoid unpleasant reactions, skin protection creams are commonly employed, but some fail to afford protection against a variety of xenobiotics. In this study, two skin protection creams were assayed comparatively looking for a protective effect if any against a liquid soap and an alcohol-based gel designed for hand hygiene in medical settings. METHODS: Corneosurfametry and corneoxenometry are two in vitro bioessays which were selected for their good reproducibility, sensitivity and ease of use. A Kruskal-Wallis ANOVA test followed by the Dunn test was realized to compare series of data obtained. RESULTS: Significant differences in efficacy were obtained between the two assayed skin protection creams. One of the two tested creams showed a real protective effect against mild irritants, but the other tested cream presented an irritant potential in its application with mild irritants. CONCLUSION: The differences observed for the two tested skin protection creams were probably due to their galenic composition and their possible interactions with the offending products. As a result, the present in vitro bioassays showed contrasted effects of the creams corresponding to either a protective or an irritant effect on human stratum corneum. [less ▲]

Detailed reference viewed: 224 (35 ULg)
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See detailA fatal case of bupropion (ZybanR) overdose
Mercerolle, Magali; Denooz, Raphael ULg; Lachâtre, Gérard et al

in Journal of Analytical Toxicology (2008), 32

Detailed reference viewed: 104 (55 ULg)