References of "Denooz, Raphael"
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See detailIntérêt du dosage des sulfamidés hypoglycémiants: de la vérification de la compliance au diagnostic différentiel des hyperinsulinismes
Denooz, Raphael ULg; Charlier, Corinne ULg

in Revue Médicale de Liège (2010), 65(9), 493-497

Plus de cinquante ans après leur mise sur le marché, les sulfamidés hypoglycémiants font toujours partie du traitement de base du diabète non insulinodépendant. A côté de leurs remarquables propriétés ... [more ▼]

Plus de cinquante ans après leur mise sur le marché, les sulfamidés hypoglycémiants font toujours partie du traitement de base du diabète non insulinodépendant. A côté de leurs remarquables propriétés pharmacologiques, ces produits ont, à différentes reprises, été tenus responsables d’hypoglycémies sévères survenant de manière inexpliquée chez des sujets non diabétiques. Ce type d’hypoglycémie, qualifiée de factice, est notamment fréquemment retrouvée dans le syndrome de Munchausen, trouble psychiatrique poussant le malade à mimer une pathologie. Cette hypoglycémie est associée à un hyperinsulinisme endogène simulant une pathologie endocrine très rare, l’insulinome. Nous souhaitons faire connaître aux Cliniciens l’intérêt de la recherche et du dosage sanguin des sulfamidés hypoglycémiants dans le cadre du diagnostic différentiel des hyperinsulinismes. La mise en évidence d’une hypoglycémie induite par ces médicaments constitue un élément intéressant pour orienter le diagnostic et éventuellement éviter une pancréatectomie en cas de forte suspicion d’insulinome. La méthode analytique développée au laboratoire s’appuie sur la chromatographie liquide ultra performante couplée à la spectrométrie de masse tandem (UPLC -MS -MS ). Par cette technique, on peut mettre en évidence dans le sang une dizaine de sulfonylurées et le répaglinide. Elle constituera à l’avenir une méthode de choix face à toute suspicion de mésusage des sulfonylurées. [less ▲]

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See detailQuantification in postmortem blood and identification in urine of tramadol and its two main metabolites in two cases of lethal tramadol intoxication
De Backer, Benjamin ULg; Renardy, Françoise ULg; Denooz, Raphael ULg et al

in Mitteilungsblatt der Gesellschaft für Toxikologische und Forensische Chemie (2010), 77(3), 252

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See detailFatal intoxications by acenocoumarol, phenprocoumon and warfarin: method validation in blood using the total error approach.
Denooz, Raphael ULg; Douamba, Zoénabo; Charlier, Corinne ULg

in Journal of Chromatography. B : Analytical Technologies in the Biomedical & Life Sciences (2009), 877(23), 2344-2348

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See detailGestion du contrôle de qualité interne
Denooz, Raphael ULg; Pirson, Laurence ULg

Conference (2009, February)

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See detailIntérêt du dosage des sulfamidés hypoglycémiants
DENOOZ, Raphael ULg

Scientific conference (2009, January)

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See detailScreenings Toxicologiques: Apport de l'immunoanalyse
Denooz, Raphael ULg

Conference (2008, June)

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See detailSimultaneous Determination of Five Beta-Lactam Antibiotics (Cefepim, Ceftazidim, Cefuroxim, Meropenem and Piperacillin) in Human Plasma by High-Performance Liquid Chromatography with Ultraviolet Detection
Denooz, Raphael ULg; Charlier, Corinne ULg

in Journal of Chromatography. B : Analytical Technologies in the Biomedical & Life Sciences (2008), 864(1-2), 161-7

Monitoring of plasma antibiotic drugs is useful for better clinical management in infected patients, particularly in intensive care units. A simple and sensitive high-performance liquid chromatography ... [more ▼]

Monitoring of plasma antibiotic drugs is useful for better clinical management in infected patients, particularly in intensive care units. A simple and sensitive high-performance liquid chromatography (HPLC) method with ultraviolet (UV) detection has been developed and validated for simultaneous quantification of five beta-lactam antibiotics in human plasma: cefepim, ceftazidim, cefuroxim, meropenem and piperacillin. The plasma sample, after spiked with ceforanid as an internal standard (IS), was submitted to a solid-phase extraction (SPE) prior to HPLC analysis. A chromatographic separation was achieved on a C8 symmetry column with a mobile phase consisting of an acetonitrile and phosphate buffer (pH 7.4) mixture in a gradient mode. Detection was carried out at a wavelength between 200 and 400nm. The method developed was linear over the concentration range of 2.5-60mug/mL for each antibiotic in the plasma samples. Accuracy ranged from 93.2 to 107.1% and precision was between 0.9 and 12.2%. The method has been applied to plasma samples obtained from patients treated with beta-lactam antibiotics and is appropriated for easy determination of plasma concentrations for therapeutic monitoring applications. [less ▲]

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See detailMechanisms of Interaction of Endocrine-Disrupting Chemicals with Glutamate-Evoked Secretion of Gonadotropin-Releasing Hormone
Rasier, Gregory; Parent, Anne-Simone ULg; Gerard, Arlette ULg et al

in Toxicological Sciences (2008), 102(1), 33-41

In previous studies, we detected a dichlorodiphenyltrichloroethane (DDT) derivative in the serum of children with sexual precocity after migration from developing countries. Recently, we reported that DDT ... [more ▼]

In previous studies, we detected a dichlorodiphenyltrichloroethane (DDT) derivative in the serum of children with sexual precocity after migration from developing countries. Recently, we reported that DDT stimulated pulsatile gonadotropin-releasing hormone (GnRH) secretion and sexual maturation in the female rat. The aim of this study was to delineate the mechanisms of interaction of endocrine-disrupting chemicals including DDT with GnRH secretion evoked by glutamate in vitro. Using hypothalamic explants obtained from 15-day-old female rats, estradiol (E2) and DDT caused a concentration-related increase in glutamate-evoked GnRH release while p,p'-dichlorodiphenyldichloroethene and methoxychlor had no effect. The effective DDT concentrations in vitro were consistent with the serum concentrations measured in vivo 5 days after exposure of immature rats to 10 mg/kg/day of o,p'-DDT. Bisphenol A induced some stimulatory effect, whereas no change was observed with 4-nonylphenol. The o,p'-DDT effects in vitro were prevented partially by a selective antagonist of the alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) subtype of glutamate receptors. A complete prevention of o,p'-DDT effects was caused by an estrogen receptor (ER) antagonist as well as an aryl hydrocarbon receptor (AHR) antagonist and inhibitors of protein kinases A and C and mitogen-activated kinases. While an intermittent incubation with E2 caused no change in amplification of the glutamate-evoked GnRH release for 4 h, continuous incubation with E2 or o,p'-DDT caused an increase of this amplification after 3.5 h of incubation. In summary, DDT amplifies the glutamate-evoked GnRH secretion in vitro through rapid and slow effects involving ER, AHR, and AMPA receptor mediation. [less ▲]

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See detailScreenings Toxicologiques: apport d'un système HPLC/DAD
Denooz, Raphael ULg; Dubois, Nathalie ULg

Conference (2008, March)

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See detailSkin protection creams in medical settings: successful or evil ?
Xhauflaire, Emmanuelle ULg; Macarenko, Elena; Denooz, Raphael ULg et al

in Journal of Occupational Medicine and Toxicology (London, England) (2008), 3

ABSTRACT: BACKGROUND: Chronic exposure to mild irritants including cleansing and antiseptic products used for hand hygiene generates insults to the skin. To avoid unpleasant reactions, skin protection ... [more ▼]

ABSTRACT: BACKGROUND: Chronic exposure to mild irritants including cleansing and antiseptic products used for hand hygiene generates insults to the skin. To avoid unpleasant reactions, skin protection creams are commonly employed, but some fail to afford protection against a variety of xenobiotics. In this study, two skin protection creams were assayed comparatively looking for a protective effect if any against a liquid soap and an alcohol-based gel designed for hand hygiene in medical settings. METHODS: Corneosurfametry and corneoxenometry are two in vitro bioessays which were selected for their good reproducibility, sensitivity and ease of use. A Kruskal-Wallis ANOVA test followed by the Dunn test was realized to compare series of data obtained. RESULTS: Significant differences in efficacy were obtained between the two assayed skin protection creams. One of the two tested creams showed a real protective effect against mild irritants, but the other tested cream presented an irritant potential in its application with mild irritants. CONCLUSION: The differences observed for the two tested skin protection creams were probably due to their galenic composition and their possible interactions with the offending products. As a result, the present in vitro bioassays showed contrasted effects of the creams corresponding to either a protective or an irritant effect on human stratum corneum. [less ▲]

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See detailA fatal case of bupropion (ZybanR) overdose
Mercerolle, Magali; Denooz, Raphael ULg; Lachâtre, Gérard et al

in Journal of Analytical Toxicology (2008), 32

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See detailGHB measurement by GC-MS in blood, urine and gastric contents, following an acute intoxication in Belgium
Bodson, Quentin; Denooz, Raphael ULg; Serpe, Philippe ULg et al

in Acta Clinica Belgica (2008), 63

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See detailRevue des cas d'intoxication mortelle a la methadone: observations recensees au CHU de Liege
Denooz, Raphael ULg; Charlier, Corinne ULg

in Acta Clinica Belgica. Supplementum (2006), 61(Suppl. 1), 32-36

All methadone-associated deaths from October 2002 to April 2005 are analysed. A regular increase in these fatal intoxications has been recorded, in Belgium as in other countries, due, in particular, to an ... [more ▼]

All methadone-associated deaths from October 2002 to April 2005 are analysed. A regular increase in these fatal intoxications has been recorded, in Belgium as in other countries, due, in particular, to an intensified prescription of this product, of which illicit use as street dope becomes problematic. Over the 30 months period covered by the study, 26 deaths related to methadone were listed, of which 3 occurred in accidental circumstances. In the other 23 cases, methadone can be found, yet always associated with psychotropic substances, mainly benzodiazepines (18 cases), narcotics (15 cases) and finally alcohol (5 cases). Based upon the nature of the products combined with methadone, records have been divided in two groups: In the first group (17 observations), xenobiotics at (infra)therapeutic levels are detected. In the other group (6 observations), xenobiotics at high and toxic levels are detected. Blood methadone concentrations are not so different between the 2 groups of individuals since the median values and the extreme values are worth respectively 308, and 110-11300 microg/L, for the first group and 776 and 93-2080 microg/L for the second group.There is thus an important overlap between the therapeutic blood methadone concentrations (150-400 microg/L) and blood concentrations observed in fatalities.Thus, it is necessary that all information and post-mortem results must be examined in a critical way to identify and justify cause of the death. [less ▲]

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See detailIntoxications au dextropropoxyphène et au tramadol: bilan d'un an de recueil
Cohen, Sabine; Manchon, Monique; Artur, Michèle et al

in Annales de Toxicologie Analytique (2006), 2

La prescription de plus en plus fréquente du dextropropoxyphène (DP) et du tramadol, deux antalgiques opioïdes, a conduit les membres du groupe de travail "Toxicologie hospitalière" de la Société ... [more ▼]

La prescription de plus en plus fréquente du dextropropoxyphène (DP) et du tramadol, deux antalgiques opioïdes, a conduit les membres du groupe de travail "Toxicologie hospitalière" de la Société Française de Toxicologie Analytique (SFTA) à étudier leur toxicité clinique observée. L'étude a consisté en l'analyse des cas où l'une ou l'autre de ces molécules a été identifiée au décours d'un screening toxicologique réalisé durant l'année 2004 dans les laboratoires hospitaliers des membres de ce groupe. Pour chaque patient ont été recueillis les données démographiques, les concentrations plasmatiques en dextropropoxyphène, norpropoxyphène (NP) ou tramadol, la concentration plasmatique en paracétamol, les médicaments associés et les signes cliniques (digestifs, respiratoires, cardiaques et neurologiques). Aucune relation entre les concentrations plasmatiques de dextropropoxyphène et de paracétamol, ni entre celles de dextropropoxyphène ou de tramadol et la gravité clinique n'a été observée. Les concentrations plasmatiques observées en dextropropoxyphène sont faibles mais doivent attirer l'attention sur une éventuelle intoxication au paracétamol. Les concentrations plasmatiques en tramadol peuvent être élevées mais les intoxications sont dans l'ensemble peu sévères et les concentrations toxiques semblent très supérieures à la concentration de 1 mg/L. [less ▲]

Detailed reference viewed: 84 (3 ULg)