References of "Delvigne, Frank"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailNew perspectives for the design of sustainable bioprocesses for phosphorus recovery from waste
Tarayre, Cédric ULg; De Clercq, Lies; Charlier, Raphaëlle et al

in Bioresource Technology (2016), 206

Phosphate rock has long been used for the production of phosphorus based chemicals. However, considering the depletion of the reservoirs and the decrease of the quality of phosphate rocks, a potential ... [more ▼]

Phosphate rock has long been used for the production of phosphorus based chemicals. However, considering the depletion of the reservoirs and the decrease of the quality of phosphate rocks, a potential market is now emerging for the recovery of phosphate from waste and its reuse for different applications. Notably, phosphate recovery from wastewater could be included in a circular economy approach. This review focuses on the use of microbial systems for phosphorus accumulation and recovery, by considering the actual range of analytical techniques available for the monitoring of phosphorus accumulating organisms, as well as the actual biochemical and metabolic engineering toolbox available for the optimization of bioprocesses. In this context, knowledge gathered from process, system and synthetic biology could potentially lead to innovative process design. [less ▲]

Detailed reference viewed: 32 (2 ULg)
Full Text
Peer Reviewed
See detailCharacterization and Evaluation of the Potential of a Diesel-Degrading Bacterial Consortium Isolated from Fresh Mangrove Sediment
Semboung Lang, Firmin ULg; Destain, Jacqueline ULg; Delvigne, Frank ULg et al

in Water, Air & Soil Pollution (2016)

Hydrocarbons are ubiquitous and persistent organic pollutants in the environment. In wetlands and marine environments, particularly in mangrove ecosystems, their increase and significant accumulation ... [more ▼]

Hydrocarbons are ubiquitous and persistent organic pollutants in the environment. In wetlands and marine environments, particularly in mangrove ecosystems, their increase and significant accumulation result from human activities such as oil and gas exploration and exploitation operations. Remediation of these ecosystems requires the development of adequate and effective strategies. Natural attenuation, biostimulation, and bioaugmentation are all biological soil treatment techniques that can be adapted to mangroves. Our experiments were performed on samples of fresh mangrove sediments from the Cameroon estuary and mainly from the Wouri River in Cameroon. This study aims to assess the degradation potential of a bacterial consortium isolated from mangrove sediment. The principle of our bioremediation experiments is based on a series of tests designed to evaluate the potential of an active indigenous microflora and three exogenous pure strains, to degrade diesel with/without adding nutrients. The experiments were conducted in laboratory flasks and a greenhouse in microcosms. In one case, as in the other, the endogenous microflora showed that it was able to degrade diesel. Under stress of the pollutant, the endogenous microflora fits well enough in the middle to enable metabolism of the pollutant. However, the Rhodococcus strain was more effective over time. The degradation rate was 77 and 90%in the vials containing the sterile sediments and non-sterile sediments, respectively. The results are comparable with those obtained in the microcosms in a greenhouse where only the endogenous microflora were used. The results of this study show that mangrove sediment contains an active microflora that can metabolize diesel. Indigenous and active microflora show an interesting potential for diesel degradation. [less ▲]

Detailed reference viewed: 18 (2 ULg)
Full Text
Peer Reviewed
See detailCharacterization and Evaluation of the Potential of a Diesel-Degrading Bacterial Consortium Isolated from Fresh Mangrove Sediment
Lang, Firmin Semboung; Destain, Jacqueline ULg; Delvigne, Frank ULg et al

in Water, Air & Soil Pollution (2016), 227(2), 1-20

Detailed reference viewed: 14 (1 ULg)
Full Text
Peer Reviewed
See detailBiodegradation of Polycyclic Aromatic Hydrocarbons in Mangrove Sediments Under Different Strategies: Natural Attenuation, Biostimulation, and Bioaugmentation with Rhodococcus erythropolis T902.1
Lang, F. S.; Destain, Jacqueline ULg; Delvigne, Frank ULg et al

in Water, Air & Soil Pollution (2016), 227(9),

Polycyclic aromatic hydrocarbons (PAHs) are pollutants that occur in mangrove sediments. Their removal by bacteria often depends on specific characteristics as the number of benzene rings they possess and ... [more ▼]

Polycyclic aromatic hydrocarbons (PAHs) are pollutants that occur in mangrove sediments. Their removal by bacteria often depends on specific characteristics as the number of benzene rings they possess and their solubility. Their removal also depends on environmental factors, such as pH, temperature, oxygen, and the ability of the endogenous or exogenous microflora to metabolize hydrocarbons. With the aim of treating mangrove sediments polluted by hydrocarbons in a biological way, a biodegradation experiment was conducted using mangrove sediments artificially contaminated with a mixture of four PAHs. The study used Rhodococcus erythropolis as an exogenous bacterial strain in order to assess the biodegradation of the PAH mixture by natural attenuation, biostimulation, bioaugmentation, and a combination of biostimulation and bioaugmentation. The results showed that the last three treatments were more efficient than natural attenuation. The biostimulation/bioaugmentation combination proved to be the most effective PAH degradation treatment. © 2016, Springer International Publishing Switzerland. [less ▲]

Detailed reference viewed: 13 (1 ULg)
Full Text
Peer Reviewed
See detailFlow cytometry community fingerprinting and amplicon sequencing for the assessment of landfill leachate cellulolytic bioaugmentation
Kinet, Romain ULg; Dzaomuho, Phidias; Baert, Jonathan ULg et al

in Bioresource Technology (2016)

Flow cytometry (FCM) is a high throughput single cell technology that is actually becoming widely used for studying phenotypic and genotypic diversity among microbial communities. This technology is ... [more ▼]

Flow cytometry (FCM) is a high throughput single cell technology that is actually becoming widely used for studying phenotypic and genotypic diversity among microbial communities. This technology is considered in this work for the assessment of a bioaugmentation treatment in order to enhance cellulolytic potential of landfill leachate. The experimental results reveal the relevant increase of leachate cellulolytic potential due to bioaugmentation. Cytometric monitoring of microbial dynamics along these assays is then realized. The Flow FP package is used to establish microbial samples fingerprint from initial 2D cytometry histograms. This procedure allows highlighting microbial communities' variation along the assays. Cytometric and 16S rRNA gene sequencing fingerprinting methods are then compared. The two approaches give same evidence about microbial dynamics throughout digestion assay. There are however a lack of significant correlation between cytometric and amplicon sequencing fingerprint at genus or species level. Same phenotypical profiles of microbiota during assays matched to several 16S rRNA gene sequencing ones. Flow cytometry fingerprinting can thus be considered as a promising routine on-site method suitable for the detection of stability/variation/disturbance of complex microbial communities involved in bioprocesses. [less ▲]

Detailed reference viewed: 42 (12 ULg)
Full Text
Peer Reviewed
See detailDeciphering how LIP2 and POX2 promoters can optimally regulate recombinant protein production in the yeast Yarrowia lipolytica.
Sassi, Hosni; Delvigne, Frank ULg; Nicaud, Jean-Marc et al

in Microbial Cell Factories (2016), 15(1), 159

Detailed reference viewed: 24 (6 ULg)
Full Text
Peer Reviewed
See detailInfluence of methanol/sorbitol co-feeding rate on pAOX1 induction in a Pichia pastoris Mut+ strain in bioreactor with limited oxygen transfer rate.
Carly, F.; Niu, H.; Delvigne, Frank ULg et al

in Journal of industrial microbiology & biotechnology (2016), 43(4), 517-23

High Pichia pastoris biomass density could be obtained using high co-feeding rate of methanol and sorbitol in a fed-batch or continuous culture, while further higher feeding rate finally leads to oxygen ... [more ▼]

High Pichia pastoris biomass density could be obtained using high co-feeding rate of methanol and sorbitol in a fed-batch or continuous culture, while further higher feeding rate finally leads to oxygen limitation in bioreactor. In the literature, there is lack of report about AOX1 promoter regulation with regard to dissolved oxygen level (DO). Therefore, in this work, chemostat cultures were performed to investigate the cell growth, metabolism and regulation of the AOX1 promoter (pAOX1) regarding co-feeding rate of optimized methanol/sorbitol mixture (methanol fraction 0.60 C-mol/C-mol) using a P. pastoris Mut+/pAOX1-lacZ strain. The oxygen transfer rates (OTR) in bioreactor were kept in the range of typical values of large bioreactor, i.e., 4-8 g/(L h) if DO equals 30 % saturation or 5-10 g/(L h) if DO nears zero. For DO >0, an increase of the carbon fed led to an increase of pAOX1 induction. By contrast, when dissolved oxygen was completely depleted, methanol accumulated, causing a 30 % decrease of pAOX1 induction. However, this decrease is more likely to be lined to methanol accumulation than to low level of dissolved oxygen (<4 % DO). Methanol/sorbitol co-feeding allowed cells to adapt to oxygen transient limitations that often occur at industrial scale with reduced effect on pAOX1 induction. The optimal feeding rate tested here was 6.6 mmol C (DCW h)(-1) at an OTR of 8.28 g O2(L h)(-1) with over fivefold pAOX1 induction (probably directly associated with target protein productivity) compared with previous work. [less ▲]

Detailed reference viewed: 60 (7 ULg)
Full Text
See detailTechniques for nutrient recovery from manure and slurry
Camargo-Valero, Miller; De Clercq, Lies; Delvigne, Frank ULg et al

Report (2015)

Detailed reference viewed: 35 (1 ULg)
Full Text
See detailTechniques for nutrient recovery from digestate derivatives
Bamelis, Lies; Blancke, Stieven; Camargo-Valero, Miller et al

Report (2015)

Detailed reference viewed: 17 (0 ULg)
Full Text
See detailTechniques for nutrient recovery from household and industrial wastes
Camargo-Valero, Miller; Bamelis, Lies; De Clercq, Lies et al

Report (2015)

Detailed reference viewed: 18 (4 ULg)
Full Text
Peer Reviewed
See detailFungal biofilm reactor improves the quality of a fusion protein GLA::GFP produced by Aspergillus oryzae
Zune, Quentin ULg; Delepierre, Anissa ULg; Bauwens, Julien ULg et al

Poster (2015, October)

Fungal biofilm is known to promote the excretion of secondary metabolites, in accordance with solid-state related physiological mechanisms. In this work, the potentialities of fungal biofilm will be ... [more ▼]

Fungal biofilm is known to promote the excretion of secondary metabolites, in accordance with solid-state related physiological mechanisms. In this work, the potentialities of fungal biofilm will be investigated in the context of the production of a Gla::GFP fusion protein by Aspergillus oryzae. Since the production of this protein is under the control of the promoter glaB, specifically induced in solid-state fermentation, biofilm mode of culture is expected to enhance the global productivity. However, we found that the glaB promoter is also activated in submerged bioreactor and the fusion protein production is higher in this mode of culture. This result is related to the high shear stress leading to biomass autolysis and leakage of intracellular fusion protein into the extracellular medium. Moreover, 2D-gel electrophoresis highlights preservation of the fusion protein integrity produced in biofilm conditions whereas proteolysis strongly affects fusion protein recovery in the submerged cultures performed at high stirring rate. Fungal biofilm reactor design was then further investigated and the scale-up potentialities were evaluated. Indeed, the specific design investigated in this work involves the use of metal structured packing exhibiting a high specific area and that can be easily expanded to large-scale bioprocessing conditions. [less ▲]

Detailed reference viewed: 98 (24 ULg)
Full Text
Peer Reviewed
See detailEuler–Lagrange approach to model heterogeneities in stirred tank bioreactors – Comparison to experimental flow characterization and particle tracking
Delafosse, Angélique ULg; Calvo, Sébastien ULg; Collignon, Marie-Laure ULg et al

in Chemical Engineering Science (2015), 134

The aim of this work is the validation of an Euler–Lagrange modeling approach coupling a CFD-based compartment model (Eulerian approach) and a stochastic model based on a Continuous-Time Markov Chain ... [more ▼]

The aim of this work is the validation of an Euler–Lagrange modeling approach coupling a CFD-based compartment model (Eulerian approach) and a stochastic model based on a Continuous-Time Markov Chain (Lagrangian approach). The turbulent flow structure and the mixing process in a bioreactor stirred by an axial Mixel TT impeller is characterized by PIV and tracer experiments. Comparison between experimental and numerical data shows that the CFD-based compartment model is able to reproduce accurately the spatial heterogeneities inside the bioreactor. The trajectory of a small tracer particle which perfectly follows the fluid flow is measured by optical trajectography. It is then simulated by a stochastic model which is either based on an homogeneous or on an inhomogeneous Continuous Time Markov Chain (CTMC). Comparison of residence and circulation time distributions in three zones defined inside the bioreactor shows that the inhomogeneous CTMC model predicts with an excellent accuracy the particle trajectories inside the bioreactor. The modeling approach proposed here could be an useful tool to design scale-down bioreactors in order to reproduce at lab-scale the stress levels encountered in large-scale production bioreactors and to characterize and compare different bioreactor configurations. [less ▲]

Detailed reference viewed: 62 (29 ULg)
Full Text
Peer Reviewed
See detailFluorescent reporter libraries as useful tools for optimizing microbial cell factories: a review of the current methods and applications
Delvigne, Frank ULg; Pêcheux, Hélène; Tarayre, Cédric ULg

in Frontiers in Bioengineering and Biotechnology (2015), 3

The use of genetically encoded fluorescent reporters allows speeding up the initial optimization steps of microbial bioprocesses. These reporters can be used for determining the expression level of a ... [more ▼]

The use of genetically encoded fluorescent reporters allows speeding up the initial optimization steps of microbial bioprocesses. These reporters can be used for determining the expression level of a particular promoter, not only the synthesis of a specific protein but also the content of intracellular metabolites. The level of protein/metabolite is thus proportional to a fluorescence signal. By this way, mean expression profiles of protein/ metabolites can be determined non-invasively at a high-throughput rate, allowing the rapid identification of the best producers. Actually, different kinds of reporter systems are available, as well as specific cultivation devices allowing the on-line recording of the fluorescent signal. Cell-to-cell variability is another important phenomenon that can be integrated into the screening procedures for the selection of more efficient microbial cell factories. [less ▲]

Detailed reference viewed: 25 (1 ULg)
Full Text
Peer Reviewed
See detailLa lutte contre les moustiques (Diptera: Culicidae): diversité des approches et application du contrôle biologique
Bawin, Thomas ULg; Seye, Fawrou; Boukraa, Slimane ULg et al

in Canadian Entomologist (2015), 147(4), 476-500

Many mosquito (Diptera: Culicidae) species are zoonotic vectors responsible for numerous infectious diseases of medical and veterinary importance. Currently, changes in the vectors’ geographical ... [more ▼]

Many mosquito (Diptera: Culicidae) species are zoonotic vectors responsible for numerous infectious diseases of medical and veterinary importance. Currently, changes in the vectors’ geographical distribution induced chiefly by anthropogenic factors are accompanied by emerging and reemerging infectious diseases in Europe and North America. Since the advent of synthetic insecticides during the Second World War, mosquitoes are the object of considerably expanded and deepened research. In an integrated pest management context, means of control are now mainly classified as: (1) environmental management and physical control, (2) chemical control, (3) genetic control, and (4) biological control by means of entomophagous predators and entomopathogenic microorganisms. In this context, these last have significant potential because of their ability to infect and kill their host with more or less targeted selectivity. This article proposes to emphasize biological control among other techniques in mosquito control, and to assess the potential and the opportunities offered by entomopathogenic bacteria, viruses and fungi. Finally, their use as biopesticides is discussed. [less ▲]

Detailed reference viewed: 170 (43 ULg)