References of "Deleuze, Stefan"
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See detailRaw semen concentration directly influences CASA velocity pathways
Parrilla Hernandez, Sonia ULiege; Deleuze, Stefan ULiege; Van Den Berghe, Femke ULiege et al

in Journal of Equine Veterinary Science (2012, August), 32(8), 505

We observed an influence of the stallion on volume, spermatozoa concentration and all CASA parameters (p<0.0001), which are highly dependant on each other due to geometrical association of these data ... [more ▼]

We observed an influence of the stallion on volume, spermatozoa concentration and all CASA parameters (p<0.0001), which are highly dependant on each other due to geometrical association of these data. That stallion effect may have interfered with the associations we observed as stallions seem to have specific concentration and motility pathways. More studies, with more replicates, will allow comparing results from a same stallion and further establish the correlations we report here. [less ▲]

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See detailTwo-dimensional and M-mode echocardiographic reference values in healthy adult Saanen goats
Leroux, Aurélia ULiege; Moonen, Marie ULiege; Farnir, Frédéric ULiege et al

in Veterinary Record : Journal of the British Veterinary Association (2012), 170(6), 154

Echocardiography has become a routine non invasive cardiac diagnostic tool in most species. Accurate measurement of cardiac dimensions requires reference values, which are poorly documented in goats. The ... [more ▼]

Echocardiography has become a routine non invasive cardiac diagnostic tool in most species. Accurate measurement of cardiac dimensions requires reference values, which are poorly documented in goats. The aim of this study was to test the inter-day repeatability and to establish the reference values of two-dimensional (2D-) and time-motion (M-) mode echocardiographic variables in healthy adult Saanen goats. Six goats were investigated three times by the same observer at one day interval using a standardized 2D- and M-mode echocardiographic protocol. The intra-observer inter-day repeatability was tested using analysis of variance, calculation of the coefficient of variation and confidence intervals. A single echocardiographic examination was performed in 6 other goats, and values obtained in the 12 goats were used to establish the 2D- and M-mode echocardiographic reference values in healthy adult female Saanen goats. Statistical analysis revealed a good inter-day repeatability of the echocardiographic cardiac measurements. Echocardiographic reference values obtained in healthy adult Saanen goats seemed slightly higher than those reported in healthy Swedish domestic goats and were similar to those reported in healthy adult sheep. [less ▲]

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See detailMyeloperoxidase in Equine Semen: Concentration and Localization during Freezing Processing
Ponthier, Jérôme ULiege; Desvals, Maud; Franck, Thierry ULiege et al

in Journal of Equine Veterinary Science (2012), (32), 32-37

Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in and released by neutro- phils during degranulation or after lysis. Post-thaw semen contains MPO, and concen- tration of this enzyme is associated ... [more ▼]

Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in and released by neutro- phils during degranulation or after lysis. Post-thaw semen contains MPO, and concen- tration of this enzyme is associated with decreased motility. The aim of this study was to determine kinetics of MPO concentration during freezing, its origin, and its impact on frozenethawed semen. Forty ejaculates were used. Semen was frozen using the classical freezing procedure. MPO concentrations were assayed in fresh semen, after centrifuga- tion, and after cooling down to 4 C. Post-thaw MPO assay results and spermogram characteristics were determined. MPO immunocytochemistry was performed on 4 different ejaculates at each step of freezing procedure. MPO concentration increased after cooling down to 4 C and thawing compared with fresh semen. As temperature decreased, MPO was higher or tended to be higher in post-thaw poor quality samples. Nonsperm cells showed various degrees of MPO immunostaining and were observed as epithelial cells with nuclear pyknosis and keratinization. MPO immunostaining increased in medium and decreased in nonsperm cells during freezing. Our study shows that MPO concentration in equine semen increases when temperature decreases. We hypothesize that nonsperm cells present in fresh semen could release MPO. [less ▲]

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See detailSperm quality analysis in XX, XY and YY males of the Nile tilapia (Oreochromis niloticus).
Gennotte, Vincent ULiege; François, Ekniel; Rougeot, Carole ULiege et al

in Theriogenology (2012)

In Nile tilapia (Oreochromis niloticus), individuals with atypical sexual genotype are commonly used in farming (use of YY males to produce all-male offsprings), but they also constitute major tools to ... [more ▼]

In Nile tilapia (Oreochromis niloticus), individuals with atypical sexual genotype are commonly used in farming (use of YY males to produce all-male offsprings), but they also constitute major tools to study sex determinism mechanisms. In other species, sexual genotype and sex reversal procedures affect different aspects of biology such as growth, behaviour and reproductive success. The aim of this study was to assess the influence of sexual genotype on sperm quality in Nile tilapia. Milt characteristics were compared in XX (sex-reversed), XY and YY males in terms of gonadosomatic index, sperm count, sperm motility and duration of sperm motility. Sperm motility was measured by computer-assisted sperm analysis (CASA) quantifying several parameters: total motility, progressive motility, curvilinear velocity, straight line velocity, average path velocity and linearity. None of the sperm trait measured differed significantly between the three genotypes. Mean values of gonadosomatic index, sperm concentration and sperm motility duration of XX, XY and YY males respectively ranged from 0.92 to 1.33 %, from 1.69 to 2.22 × 10(9) cells mL-1 and from 18’04’’ to 27’32’’. Mean values of total motility and curvilinear velocity 1 min after sperm activation respectively ranged from 53 to 58 % and from 71 to 76 µm s-1 for the three genotypes. After 3 min of activity, all the sperm motility and velocity parameters dropped by half and continued to slowly decrease thereafter. Seven min after activation, only 9 to 13 % of spermatozoa were still progressive. Our results prove that neither sexual genotype nor hormonal sex reversal treatments affect sperm quality in male Nile tilapias with atypical sexual genotype. [less ▲]

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See detailChanges in histone H4 acetylation during in vivo versus in vitro maturation of equine oocytes
Franciosi, Federica; Lodde, Valentina; Goudet, Ghylène et al

in Molecular Human Reproduction (2011)

Epigenetic modifications are established during gametogenesis and preimplantation embryonic development. Any disturbance of the normal natural environment during these critical phases could cause ... [more ▼]

Epigenetic modifications are established during gametogenesis and preimplantation embryonic development. Any disturbance of the normal natural environment during these critical phases could cause alterations of the epigenetic signature. Histone acetylation is an important epigenetic modification involved in the regulation of chromatin organization and gene expression. The present study was aimed to determine whether the proper establishment of post-translational histone H4 acetylation at lysine 8 (AcH4K8), 12 (AcH4K12), and 16 (AcH4K16) of equine oocytes is adversely affected during in vitro maturation when compared to in vivo matured oocytes collected from naturally cycling mares not undergoing ovarian hyperstimulation. The acetylation patterns were investigated by means of indirect immunofluorescence staining with specific antibodies directed against the acetylated lysine residues. Our results indicate that the acetylation state of H4 is dependent on the chromatin configuration in immature GV stage oocytes and it changes in a residue-specific manner along with the increase of chromatin condensation. In particular, the levels of AcH4K8 and AcH4K12 increased significantly, while AcH4K16 decreased significantly from the fibrillar to the condensed state of chromatin configuration within the GV. Moreover, during meiosis K8 and K12 were substantially deacetylated without any differences between in vivo and in vitro conditions, while K16 displayed a strong acetylation in oocytes matured in vivo, and in contrast, it was markedly deacetylated following in vitro maturation. Although the functional meaning of residue-specific acetylation during oocyte differentiation and meiotic resumption needs further investigation, our results support the hypothesis that in vitro maturation conditions can adversely affect oocyte ability to regulate the epigenetic reprogramming, critical for successful meiosis and subsequent embryonic development. [less ▲]

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See detailDystocies équines: Données descriptives et approche de la gestion des soins intensifs
Deleuze, Stefan ULiege; de la Rebière de Pouyade, Geoffroy ULiege; Sandersen, Charlotte ULiege et al

Conference (2011, December 02)

La fréquence de fourbures rencontrées après une dystocie semble faible par rapport à la littérature. L’ajout d’héparines fractionnées aux traitements habituels de la métrite du post-partum (ocytocine ... [more ▼]

La fréquence de fourbures rencontrées après une dystocie semble faible par rapport à la littérature. L’ajout d’héparines fractionnées aux traitements habituels de la métrite du post-partum (ocytocine, lavage utérins, antibiotiques et anti-inflammatoires) semble intéressant pour réduire le risque de fourbure. [less ▲]

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See detailGestion du poulinage
Ponthier, Jérôme ULiege; Deleuze, Stefan ULiege

Conference given outside the academic context (2011)

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See detailEffect of Deferoxamine Mesylate on Freezability of Blood Supplemented Canine Semen
Van Den Berghe, Femke ULiege; Rijsselaere, Tom; Van Soom, Ann et al

Poster (2011, September)

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See detailSuperovulation in the mare with commercially available pFSH
Parilla Hernandez, Sonia; Deleuze, Stefan ULiege; Beckers, Jean-François ULiege et al

in Reproduction in Domestic Animals (2011), 46(s3), 138

Further studies on Stimufol with larger numbers, should be conducted as it might prove inexpensive and easy to superovulate mares.

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See detailTwo-Dimensional, M-Mode and Pulsed Wave Doppler Echocardiographic Reference Values in Healthy Adult Saanen Goats
Leroux, Aurélia ULiege; Moonen, Marie ULiege; Farnir, Frédéric ULiege et al

Poster (2011, March 18)

Echocardiography has become a routine non invasive cardiac diagnostic tool in most species. Accurate measurement of cardiac dimensions requires reference values, which are poorly documented in goats ... [more ▼]

Echocardiography has become a routine non invasive cardiac diagnostic tool in most species. Accurate measurement of cardiac dimensions requires reference values, which are poorly documented in goats. Goats are animals easy to handle with a body and heart size comparable to humans’. This makes goats an attractive candidate for the development of animal models for human cardiology research. The aim of this study was to test the repeatability and to establish the reference values of bi-dimensional (2D-), time-motion (M-) mode and pulsed wave (PW) Doppler echocardiographic variables in adult goats. Six healthy female adult Saanen goats were investigated three times by the same observer at one day interval using a standardized 2D-, M-mode and PW Doppler echocardiographic protocol. Calculation of the coefficient of variation for each variable measured within day and depending on the day allowed to evaluate their degree of variability. A single echocardiographic examination was performed in 6 other goats by the same observer, and the obtained values were added to these obtained on the third day of the 6 first goats. Then the observed mean, the standard deviation and the range of these measurements were calculated to establish the reference values of echocardiographic parameters in unsedated adult healthy female Saanen goats. Statistical analysis revealed a good inter-day repeatability of the 2D- and M-mode echocardiographic cardiac measurements, but PW Doppler parameters presented moderate to high variability, as documented in other species. Echocardiographic reference values obtained in healthy adult Saanen goats were similar to those reported in healthy adult sheep and in healthy adult humans. [less ▲]

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See detailGestion de la jument poulinière et de l'étalon
Ponthier, Jérôme ULiege; Deleuze, Stefan ULiege

Conference given outside the academic context (2011)

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See detailCCDC39 is required for assembly of inner dynein arms and the dynein regulatory complex and for normal ciliary motility in humans and dogs.
Merveille, Anne-Christine ULiege; Davis, Erica E; Becker-Heck, Anita et al

in Nature Genetics (2011), 43(1), 72-8

Primary ciliary dyskinesia (PCD) is an inherited disorder characterized by recurrent infections of the upper and lower respiratory tract, reduced fertility in males and situs inversus in about 50% of ... [more ▼]

Primary ciliary dyskinesia (PCD) is an inherited disorder characterized by recurrent infections of the upper and lower respiratory tract, reduced fertility in males and situs inversus in about 50% of affected individuals (Kartagener syndrome). It is caused by motility defects in the respiratory cilia that are responsible for airway clearance, the flagella that propel sperm cells and the nodal monocilia that determine left-right asymmetry. Recessive mutations that cause PCD have been identified in genes encoding components of the outer dynein arms, radial spokes and cytoplasmic pre-assembly factors of axonemal dyneins, but these mutations account for only about 50% of cases of PCD. We exploited the unique properties of dog populations to positionally clone a new PCD gene, CCDC39. We found that loss-of-function mutations in the human ortholog underlie a substantial fraction of PCD cases with axonemal disorganization and abnormal ciliary beating. Functional analyses indicated that CCDC39 localizes to ciliary axonemes and is essential for assembly of inner dynein arms and the dynein regulatory complex. [less ▲]

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See detailLa spermatogenèse, la libido et la dose
Ponthier, Jérôme ULiege; Lecrenier, Marie-Caroline; Parrilla Hernandez, Sonia ULiege et al

Conference given outside the academic context (2010)

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See detailAssociation between Myeloperoxidase concentration in equine frozen semen and post thawing parameters
Ponthier, Jérôme ULiege; Franck, Thierry ULiege; Detilleux, Johann ULiege et al

in Reproduction in Domestic Animals (2010), 45(5), 811-816

Despite improvement of techniques, semen of 20% of stallions remains unfreezable. Recent studies focused on impact of reactive oxygen species and oxidant enzymes on semen characteristics. Myeloperoxidase ... [more ▼]

Despite improvement of techniques, semen of 20% of stallions remains unfreezable. Recent studies focused on impact of reactive oxygen species and oxidant enzymes on semen characteristics. Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in and released by neutrophils during degranulation or after cell lysis. It is responsible for the formation of hypochlorous acid, a strong oxidant agent which could damage spermatozoa. The aim of this study was to determine the relation between MPO concentration and characteristics of frozen semen from stallions. Thirty five straws from different stallions were analyzed. Post-thawing spermatozoal concentration, progressive and total motility were determined by CASA. Freezability was determined according to post-thawing progressive motility (over or under 15%). Percent of alive spermatozoa and abnormal forms were determined after Eosin-Nigrosin and Diff-Quick® staining respectively. Post-thawing MPO concentration was measured by ELISA. Our study shows that frozen thawed semen contains large amounts of free MPO. We also observed that post-thawing MPO ELISA assay can be used as an indicator of equine semen freezability. High MPO concentration samples showed lower total and progressive motility. A higher proportion of abnormal head shape associated with acrosome reaction was observed in our late examinations of the high concentration MPO group. Our results show that MPO adversely affects total and progressive motility of equine semen. A negative correlation between normal motile forms and MPO concentration was also observed. The effect of MPO on dead or abnormal forms remains to be precised. [less ▲]

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See detailCytological diagnosis of endometritis in the mare: a comparative study
Daspet, Sarah-Morgane; Ponthier, Jérôme ULiege; Jolly, Sandra ULiege et al

in Reproduction in Domestic Animals (2010, September), 45s3

It was concluded that the brush swab was a promising diagnostic tool for use in field conditions.

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See detailComparison of the Repeatability of Echocardiographic Measurements from Different Modes and Views in Horses of Various Breeds and Sizes
Al Haidar, Ali; Farnir, Frédéric ULiege; Deleuze, Stefan ULiege et al

in Journal of Equine Veterinary Science (2010), 30(6), 287-297

The objective of this study was to compare the repeatability of echocardiographic measurements obtained from different echocardiographic modes and views in healthy adult equids of various sizes, breeds ... [more ▼]

The objective of this study was to compare the repeatability of echocardiographic measurements obtained from different echocardiographic modes and views in healthy adult equids of various sizes, breeds and thorax shapes. Ten, free of cardiac disease, equids (body weight: 120 to 662 kg; age: 1 to 26 years) from various breeds were used in this study. Each animal was submitted to a standardized echocardiographic and Doppler protocol 3 times at 1 day interval. This protocol included the measurements of left and right ventricular, aortic, pulmonary, and left atrial parameters obtained from different views using the bidimensional (2D) or the motion (M) modes, and the measurement of several parameters of blood flow obtained from the pulsed wave Doppler mode. Repeatability of each measurement was estimated on the basis of the residual variance using a linear model and the coefficient of variation of repeated measurements. A two by two comparison of the repeatability of measurements performed in different views was performed using the residual variances in a variance ratio F test. Results showed that repeatability of echocardiographic or Doppler measurements in equids of various sizes, breeds and thorax shapes are comparable to previously reported results in thoroughbred and standardbred horses. Left ventricular morphologic parameters showed a good repeatability in the classic M-mode right parasternal short axis (Sax) view at the level of the chordae tendinae, but the 2D-mode right parasternal long axis (LAx) four- chambers view appeared to offer an interesting alternative to measure them. This latter view also allowed obtaining the mostre repeatable measurement of right ventricular internal diameter. The left atrial diameter was maximal and most repeatable in the 2D-mode left parasternal long axisLAx four- chambers angled view, and the repeatability of the aortic diameter was best in the 2D-mode right parasternal long axisLAx five-chambers view. Finally, aortic systolic time intervals were more repeatable when measured from the Doppler mode as compared to the M-mode. ThusIn conclusion, best repeatability of echocardiographic measurements in horses could be optimized following an echocardiographicthe following protocol in equids: 1-M-mode right parasternal short axis view at the level of chordae tendinae, to measure left ventricular morphologic parameters, and 2D-mode right parasternal long axis four-chamber as an alternative view. 2-2D-mode right parasternal long axis five-chambersre, to measure the aortic diameter. 3-2D-mode left parasternal long axis four-chambersre angled view, to measure the left atrial diameter. [less ▲]

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See detailDescriptive study of 32 cases of doxycycline-overdosed calves.
Brihoum, Mounir ULiege; Amory, Hélène ULiege; Desmecht, Daniel ULiege et al

in Journal of Veterinary Internal Medicine (2010), 24(5), 1203-10

BACKGROUND: Reports of doxycycline-induced toxicity are limited despite common use of this antibiotic to treat infectious respiratory disorders in calves. OBJECTIVE: To describe previously unreported ... [more ▼]

BACKGROUND: Reports of doxycycline-induced toxicity are limited despite common use of this antibiotic to treat infectious respiratory disorders in calves. OBJECTIVE: To describe previously unreported kidney lesions and diagnostic test results in doxycycline-overdosed calves and to compare these results with other findings reported previously. ANIMALS: Thirty-two calves that presented with adverse effects after receiving high doses of doxycycline as a treatment for mild respiratory disorders. METHOD: Retrospective review of medical records. RESULTS: Clinical examination identified mainly lethargy, dyspnea, cough, tongue paresia or paralysis associated with dysphagia and sialorrhea, tachycardia, tachypnea, and signs of myopathy. Blood analysis indicated increases in creatine kinase, lactate dehydrogenase, aspartate aminotransferase, and sorbitol dehydrogenase activities and increased serum creatinine and urea concentrations. ECG recordings and Doppler echocardiography examination identified ventricular premature beats and a decrease in left ventricular global and systolic function, respectively. Necropsy and histopathology disclosed necrosis of the myocardium, tongue, and some striated muscles, acute renal tubular necrosis, and fatty degeneration or congestion of the liver. CONCLUSIONS: Most of these findings corroborate previous observations made in doxycycline-overdosed calves, and further suggest myocardial and striated muscular toxicity as well as renal toxicity in doxycycline-overdosed calves. [less ▲]

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See detailIs neutrophil elastase associated with myeloperoxidase concentration and post-thawing parameters in equine frozen semen?
Ponthier, Jérôme ULiege; de la Rebière de Pouyade, Geoffroy ULiege; Desvals, Maud et al

in Animal Reproduction Science (2010), 121S

Non sperm cells concentration is higher in unfreezable semen. A relation between non-sperm cells in fresh semen and MPO and post thawing quality has been observed. Neutrophil Elastase seems to have no ... [more ▼]

Non sperm cells concentration is higher in unfreezable semen. A relation between non-sperm cells in fresh semen and MPO and post thawing quality has been observed. Neutrophil Elastase seems to have no effect on semen characteristics and to be not associated with on-sperm cells and freezability. [less ▲]

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See detailCysteamine supplementation of in vitro maturation media: a review.
Deleuze, Stefan ULiege; Goudet, G.

in Reproduction in Domestic Animals (2010), 45(6), 476-82

Under in vitro culture conditions, oxidative modifications of cell components via increased reactive oxygen species (ROS) represent a major culture induced stress. Anti-oxidant systems such as glutathione ... [more ▼]

Under in vitro culture conditions, oxidative modifications of cell components via increased reactive oxygen species (ROS) represent a major culture induced stress. Anti-oxidant systems such as glutathione (GSH) can attenuate the deleterious effects of oxidative stress by scavenging ROS. It has been suggested that GSH content in oocytes may serve as a reservoir protecting the zygote and the early embryos from oxidative damage before genomic activation and de novo GSH synthesis occur. Addition of low molecular weight compounds to culture media, such as cysteamine, can increase GSH levels by increasing cysteine uptake. Quite naturally, effects of supplementation of in vitro maturation (IVM) media with low molecular weight thiols have been studied in various species. This article reviews the use of cysteamine supplementation for IVM, its effects on maturation rates and further embryo development. [less ▲]

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See detailInfluence of cysteamine on in vitro maturation, in vitro and in vivo fertilization of equine oocytes
Deleuze, Stefan ULiege; Dubois, Clotilde; Caillaud, Maud et al

in Reprod Domest Anim (2010), 45(1), 1-7

Contents The effect of cysteamine on in vitro nuclear and cytoplasmic maturation of equine oocytes collected by transvaginal ultrasound guided follicular aspiration was assessed. Oocytes were matured in ... [more ▼]

Contents The effect of cysteamine on in vitro nuclear and cytoplasmic maturation of equine oocytes collected by transvaginal ultrasound guided follicular aspiration was assessed. Oocytes were matured in vitro with (cysteamine group) or without (control group) cysteamine. The nuclear stage after DNA Hoechst staining, penetration rates after two different in vitro fertilization (IVF) techniques (IVF media with ionophore and Hepes buffer with heparin) and the embryo yield following oocyte intra-oviductal transfer were used as a criterion for assessing nuclear and cytoplasmic maturation, respectively. Contrary to the data described in other domestic species, there was no effect of cysteamine on in vitro nuclear maturation, IVF or in vivo embryonic development under our conditions. Ovum pick up yields (52%) and maturation rates (control group: 47% and cysteamine group: 55%) were similar to those previously reported. From 57 oocytes transferred to the oviduct in each group, the number of embryos collected was 10 (17%) in the control group and five in the cysteamine group (9%). Those two percentages were not statistically different (p > 0.05). No effect of IVF technique was seen on the success rate (6%) in each group. [less ▲]

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