References of "Delaplace, Pierre"
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See detailAntioxidants involvement in the Ageing of Non-Green Organs
Delaplace, Pierre ULg; Fauconnier, Marie-Laure ULg; du Jardin, Patrick ULg

in Gupta, S. Dutta (Ed.) Reactive oxygen species and antioxidants in higher plants (2010)

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See detailOxylipin profiles of Solanum esculentum and Solanum pennellii under salt stress conditions
Ghars, Mohamed Ali ULg; Frettinger, P.; Ghanem, M. E. et al

Poster (2009, June 05)

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See detailEvaluation of two protein extraction protocols for Pichia anomala proteome analysis.
Bajji, Mohammed; Delaplace, Pierre ULg; Mauro, Sergio et al

Poster (2009, May 19)

Pichia anomala (strain Kh6) was isolated from the surface of apple fruits and selected for its high and reliable biocontrol activity against Botrytis cinerea and Penicillium expansum. Its main modes of ... [more ▼]

Pichia anomala (strain Kh6) was isolated from the surface of apple fruits and selected for its high and reliable biocontrol activity against Botrytis cinerea and Penicillium expansum. Its main modes of action have until now been studied using essentially microbiological and molecular approaches. The study continues now using the proteomic approach and considering the in situ P. anomala/B. cinerea/apple interaction. Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) is one of the most powerful tools used for proteomic analysis. It combines two sequential separation steps, the first dimension via isoelectric focusing (IEF) and the second one by SDS PAGE. Although recent advances in 2-D PAGE, the extraction of the whole proteome and the removal of interfering contaminants still limit its application. Sample preparation constitutes indeed a critical influential step for IEF which in turn affects 2-D gel quality. The objective of the present work was thus to develop an effective protein extraction protocol designed for 2-D PAGE analysis of the proteome of P. anomala strain Kh6. As a starting point, two contrasting protein extraction protocols were chosen to be evaluated in terms of protein yield and one-dimensional (1-D) SDS PAGE and 2-D PAGE gel patterns. The first protocol uses a urea/thiourea-based lysis buffer whereas the second protocol utilizes a hot SDS-based lysis buffer with an additional precipitation step. The comparison model used consisted of apples treated with strain Kh6 alone (K) and apples first treated with Kh6 and then inoculated with B. cinerea conidia (KB). Growth kinetics of strain Kh6 on wounded apples was determined and found to be not affected by the presence of B. cinerea conidia. Proteins were extracted from yeast pellets collected at both the exponential and stationary phases of strain Kh6 growth on apples. The evaluation of both extraction protocols indicates that more proteins were extracted with the SDS protocol and, according to 1-D assays, higher molecular weight proteins were obtained with the ‘urea/thiourea’ protocol and, regardless of the protocol used, more bands were obtained during the exponential phase. 2-D assays are currently underway and the corresponding results will be presented. [less ▲]

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See detailPlant and their soil environment
Delaplace, Pierre ULg

Conference (2009)

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See detailLipoxygenase pathway and antioxidant system in salt-stressed tomato seedlings (Lycopersicon esculentum Mill.).
Delaplace, Pierre ULg; Frettinger, P.; Ghanem, M. E. et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2009), 13

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See detailPotato (Solanum Tuberosum L.) Tuber Ageing Induces Changes In The Proteome And Antioxidants Associated With The Sprouting Pattern
Delaplace, Pierre ULg; Fauconnier, Marie-Laure ULg; Sergeant, K. et al

in Journal of Experimental Botany (2009), 60(4), 1273-1288

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See detailWheat root ecophysiology: understanding communication between wheat root system and soil microorganisms.
Delaplace, Pierre ULg; d'Ans, Séverine ULg; Wathelet, Jean-Paul ULg et al

Poster (2009)

Studies leading to a better understanding of the mechanisms involved in the communication between soil microorganisms and root system can potentially lead to improved fertility management strategies. Up ... [more ▼]

Studies leading to a better understanding of the mechanisms involved in the communication between soil microorganisms and root system can potentially lead to improved fertility management strategies. Up to now, the characterization of such interactions has been mainly focused on root exsudates but volatile organic compounds (VOC) also play a role as chemical messengers in positive interactions occurring in the rhizosphere. We aim to better understand the VOC-mediated chemical ecophysiology of soil. Wheat (Triticum aestivum L.) was chosen in this project based on its importance for food production and industrial applications. [less ▲]

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See detailevaluation of two protein extractions protocols for pichia anomala proteome analysis
Bajji, M.; Delaplace, Pierre ULg; Mauro, S. et al

Poster (2009)

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See detailChanges In Oxylipin Synthesis After Phytophthora Infestans Infection Of Potato Leaves Do Not Correlate With Resistance
Fauconnier, Marie-Laure ULg; Rojas-Beltran, J.; Dupuis, B. et al

in Plant Physiology and Biochemistry (2008), 46(8-9), 823-831

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See detailMethodes de mesure de l'age physiologique des tubercules semences de pomme de terre (Solanum tuberosum L.).
Delaplace, Pierre ULg; Fauconnier, Marie-Laure ULg; Du Jardin, Patrick ULg

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2008), 12(2), 171-184

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See detailCoelogyne pulverula or Coelogyne tomentosa
Delaplace, Pierre ULg

in The Journal of the Orchid Society of Great Britain (2008), 57

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See detailOxylipin Profile And Antioxidant Status Of Potato Tubers During Extended Storage At Room Temperature
Delaplace, Pierre ULg; Rojas-Beltran, J.; Frettinger, P. et al

in Plant Physiology and Biochemistry (2008), 46(12), 1077-1084

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See detailPotato (Solanum Tuberosum L.) Tuber Physiological Age Index Is A Valid Reference Frame In Postharvest Ageing Studies
Delaplace, Pierre ULg; Brostaux, Yves ULg; Fauconnier, Marie-Laure ULg et al

in Postharvest Biology & Technology (2008), 50(1), 103-106

Essential yield components of potato crops are influenced by the physiological age of the tubers at planting. Previous studies have attempted to develop biophysical, physiological or biochemical markers ... [more ▼]

Essential yield components of potato crops are influenced by the physiological age of the tubers at planting. Previous studies have attempted to develop biophysical, physiological or biochemical markers of potato tuber ageing. This research note focuses on characterising a physiological age index (PAI) based on sprouting parameters and originally developed by Caldiz et al. [Caldiz, D.O., Fernandez, L.V., Struik, P.C., 2001. Physiological age index: a new, simple and reliable index to assess the physiological age of seed potato tubers based on the haulm killing date and length of the incubation period. Field Crop. Res. 69, 69–79]. The PAI progression of two cultivars (‘Bintje’ and ‘Désirée’) was measured and modelled over 2 harvest years. The preliminary results were consistent with other ageing markers such as the incubation period (IP) and desprouting sensitivity. Under our experimental conditions, the PAI allowed us to describe cultivar-specific and harvest year-specific age variations measured from the time the haulm (foliage) was killed and the mother plant's influence on the tubers was no longer present. By contrast, the IP data displayed a higher inherent variability that impaired their discrimination ability. Critical PAI values corresponding to the main postharvest developmental steps (e.g., dormancy, apical dominance, multiple sprouting) were then identified on the basis of emergence pattern data, allowing us to propose this index as a useful reference frame for future ageing studies. [less ▲]

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See detailThe role of oxylipins and antioxidants on off-flavor precursor formation during potato flake processing.
Gosset, Virginie; Göbel, Cornelia; Laine, Gaëlle et al

in Journal of Agricultural and Food Chemistry (2008), 56

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See detailCaractérisation physiologique et biochimique du processus de vieillissement du tubercule de pomme de terre (Solanum tuberosum L.)
Delaplace, Pierre ULg

Doctoral thesis (2007)

Potato seed tuber is considered as a model organ to study plant ageing. Besides, its physiological age greatly influences germination pattern and thus agronomical performance. In order to unravel the ... [more ▼]

Potato seed tuber is considered as a model organ to study plant ageing. Besides, its physiological age greatly influences germination pattern and thus agronomical performance. In order to unravel the biochemical processes underlying ageing, potato seed tubers (cv. Bintje and Désirée) were stored at 4°C for 7 months and regularly sampled to assess both physiological and biochemical parameters. Physiological age markers (PAI, incubation period) evolution was measured and allowed us to define a reference frame more accurate than the chronological age. A correlation has also been established with the growth parameters (number of stems, duration before emergence). In parallel, two biochemical approaches allowed us to assay proteome and antioxidant system modifications occurring during ageing. Protein pattern evolution was assessed by DIGE after hot SDS extraction. During ageing, 93 spots were strictly up- or down- regulated while 4 others exhibited an abundance curve with a transient maximum. After manual picking of 43 selected spots based on their expression profile, 32 proteins were identified by MS/MS. Differentially expressed proteins are mainly involved in patatin catabolism, stress response, glycolysis, protein turn-over and lipid oxidation. A complementary targeted approach has then been set up based on the free radical ageing theory. SOD, CAT, APX and POX activities as well as ascorbate, glutathione, carotenoid and phenolic compound contents were measured during ageing. These data seem to indicate that the antioxidant system undergoes marked changes in response to an increased production of reactive oxygen species. However, no accumulation of oxidative damages has been observed on lipids and proteins. All these kinetic data allowed us to build an integrated ageing model. These results are discussed in the above-mentioned theoretical context. [less ▲]

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See detailMyzus persicae Sulzer aphid contains oxylipins that originate from phloem sap.
Harmel, Nicolas; Delaplace, Pierre ULg; Blée, Elizabeth et al

in Journal of Plant Interactions (2007), 2

Detailed reference viewed: 35 (13 ULg)