Cystatin C is a reliable marker for estimation of glomerular filtration rate in renal transplantation: validation of a new turbidimetric assay using monospecific sheep antibodies

in Clinical Chemistry & Laboratory Medicine (2011), 49(2), 265-70

Background: The potential use of Cystatin C was recently assessed in kidney transplantation. A new particle-enhanced turbidimetric immunoassay (PETIA) that uses sheep antibodies (Binding Site Human ... [more ▼]

Background: The potential use of Cystatin C was recently assessed in kidney transplantation. A new particle-enhanced turbidimetric immunoassay (PETIA) that uses sheep antibodies (Binding Site Human Cystatin C immunoassay) has been developed. Analytical performance of this new assay was evaluated. Clinical relevance was determined by comparison with a reference method in a cohort of kidney transplant patients. Patients and methods: First, the analytical performance of the Binding Site cystatin C kit was tested on SPAPLUS® and Hitachi® analyzers. Second, a comparison study was performed using SPAPLUS® analyzer against two other cystatin C methods (the Siemens-PENIA method on BNII® and the Dako-PETIA application on Olympus AU640®). Third, the glomerular filtration rate (GFR) was estimated using several predictive cystatin C- and creatinine-based equations and compared to GFR measured by an isotopic method (99mTc-DTPA). These predictive algorithms were analyzed with respect to bias, precision and accuracy. Results: Total intra-assay and inter-assay coefficients of variation were below 5%. Values obtained with the SPAPLUS® correlated with the Siemens-PENIA and the Dako-PETIA methods. The creatinine and cystatin C-based equation allowed reliable assessment of GFR in our population of renal transplantation. Conclusions: The use of algorithms based on cystatin C and creatinine could provide a reliable estimate of GFR in kidney transplantation. [less ▲]

Recommandations pour le choix et l'harmonisation des techniques de dosage de la créatinine

in Annales de Biologie Clinique (2011), 69(1), 9-16

En 2010, un groupe de travail mixte constitué de la Société française de biologie clinique (SFBC) et de la Société de nephrologie (SN) a formulé les propositions suivantes afin de réactualiser les ... [more ▼]

En 2010, un groupe de travail mixte constitué de la Société française de biologie clinique (SFBC) et de la Société de nephrologie (SN) a formulé les propositions suivantes afin de réactualiser les recommanadations pour le dosage de la créatinine plasmatique. [less ▲]

A multicentric evaluation of IDMS-traceable creatinine enzymatic assays

in Clinica Chimica Acta (2011), 412

Chronic kidney disease definition is based on glomerular filtration rate (GFR) estimations which are derived from creatinine-based equations. The accuracy of GFR estimation is thus largely dependent of ... [more ▼]

Chronic kidney disease definition is based on glomerular filtration rate (GFR) estimations which are derived from creatinine-based equations. The accuracy of GFR estimation is thus largely dependent of those of serum creatinine assays. International recommendations highlight the need for traceable creatinine assays. The French Society of Clinical Biochemistry conducted a study for measuring accuracy of creatinine enzymatic methods. This evaluation involved 25 clinical laboratories. Creatinine was measured in serum pools ranging from 35.9±0.9 μmol/L to 174.5±3.1 μmol/L (IDMS determination) using 12 creatinine enzymatic methods. For all creatinine values greater than 74.4±1.4 μmol/L, the bias and imprecision did not exceed 5% and 5.9%, respectively. For the lowest value (35.9±0.9 μmol/L), the bias ranged from −1.8 to 9.9% (with one exception). At this level, the imprecision ranged from 1.9 to 7.8%. The true performances of the assays (couples of bias and relative standard deviation), were evaluated using Monte-Carlo simulations. Most of the assays fall within the maximum Total Error of 12% at all concentrations. This study demonstrates substantial improvements in the calibration, traceability and precision of the enzymatic methods, reaching the NKDEP recommendations. Moreover, most of these assays allowed accurate creatinine measurements for creatinine levels lower than 40 μmol/L. [less ▲]

Cystatin C as a GFR Marker in Renal Transplantation: Promises and Challenges.

in Cohen, J. B.; Ryseck, L. P. (Eds.) Cystatins: Protease Inhibitors, Biomarkers and Immunomodulators (2011)

Exploration de la fonction glomérulaire rénale (I). Méthodes de référence et créatinine sérique.

Book published by EMC, Elsevier Masson SAS (2011)

Cross-reactivity of 25-hydroxy vitamin D2 from different commercial immunoassays for 25-hydroxy vitamin D: an evaluation without spiked samples.

in Clinical chemistry and laboratory medicine : CCLM / FESCC (2011), 49(3), 555-8

Analytical validation of the Liaison Calcitonin_II-Gen (DiaSorin)

in Clinical Chemistry & Laboratory Medicine (2011), 49(2), 271-275

Vascular calcifications in dialysis patients

Learning material (2010)

Les cibles thérapeutiques pour la PTH dans les nouvelles recommandations du KDIGO: la fin d'un cauchemar?

in Néphrologie & Thérapeutique (2010, September), 6(5), 424

Quels paramètres cliniques et biologiques peuvent être considérés comme prédictifs des calcifications vasculaires chez le patient hémodialysé?

in Néphrologie & Thérapeutique (2010, September), 6(5), 296