Can we use circulating biomarkers to monitor bone turnover in CKD haemodialysis patients? Hypotheses and facts
DELANAYE, Pierre ; ; et al
in Nephrology Dialysis Transplantation (2014), 29(5), 997-1004
Assessing bone turnover is a key diagnostic tool in the global management of chronic kidney disease-mineral and bone disorder (CKD-MBD). Since bone biopsy is invasive and cannot be repeated in clinical ... [more ▼]
Assessing bone turnover is a key diagnostic tool in the global management of chronic kidney disease-mineral and bone disorder (CKD-MBD). Since bone biopsy is invasive and cannot be repeated in clinical practice and because bone histomorphometry is less available due to the lack of specialized laboratories, we will focus on potential biomarkers used to assess and monitor bone turnover. After briefly reviewing the pathophysiology of bone turnover in CKD and haemodialysis patients, we will focus on the strengths and limitations of the now recommended biomarkers, i.e. parathormone and bone-specific alkaline phosphatase. We will consider the clinical and also the biological aspects of the topic and also insist on the use of these biomarkers for the monitoring, and the follow-up of the turnover in haemodialysis subjects. Finally, we will discuss some of the most promising, but still not recommended, emerging biomarkers. [less ▲]Detailed reference viewed: 149 (13 ULg)
Evaluation of automated immunoassays for 25 (OH)-vitamin D determination in different critical populations before and after standardization of the assays
CAVALIER, Etienne ; LUKAS, Pierre ; CRINE, Yannick et al
in Clinica Chimica Acta (2014), 431Detailed reference viewed: 87 (28 ULg)
Standardization of 25-hydroxyvitamin D assays: impact of vitamin-D binding protein concentrations and uremic media on the re-standardization of six different 25(OH) vitamin D immunoassays
CAVALIER, Etienne ; LUKAS, Pierre ; CRINE, Yannick et al
Poster (2014, April)Detailed reference viewed: 15 (3 ULg)
The Third/Second Generation PTH Assay Ratio as a Marker for Parathyroid Carcinoma: Evaluation Using an Automated Platform
CAVALIER, Etienne ; BETEA, Daniela ; SCHLECK, Marie-Louise et al
in Journal of Clinical Endocrinology and Metabolism (2014), 99(3), 453-7
Background: Parathyroid carcinoma (PCa) is rare and often difficult to differentiate initially from benign disease. Because PCa oversecretes amino PTH that is detected by third-generation but not by ... [more ▼]
Background: Parathyroid carcinoma (PCa) is rare and often difficult to differentiate initially from benign disease. Because PCa oversecretes amino PTH that is detected by third-generation but not by second-generation PTH assays, the normal generation PTH ( 1) is inverted in PCa (ie, 1). Objective: The objective of the investigation was to study the utility and advantages of automated generation PTH ratio measurements using the Liaison XL platform over existing manual techniques. Setting: The study was conducted at a tertiary-referral academic center. Design: This was a retrospective laboratory study. Subjects: Eleven patients with advanced PCa (mean age 56.0 y). The controls were patients with 1°-hyperparathyroidism (n 144;meanage 53.8 y), renal transplantation (n 41;meanage 50.6 y), hemodialysis (n 80; mean age 65.2 y), and healthy elderly subjects (n 40; mean age 72.6 y). Results: The median (interquartile range) generation PTH ratio was 1.16 (1.10 –1.38) in the PCa group, which was significantly higher than the control groups: 0.74 (hemodialysis, 0.71–0.75), 0.77 (renal transplant, 0.73–0.79), 0.80 (healthy elderly, 0.74–0.83), and 0.76 (1°-hyperparathyroidism, 0.74–0.78). An inverted -generation PTH ratio ( 1) was seen in 9 of 11 PCa patients (81.8%) and in 7 of 305 controls (2.3%), 3 of 80 hemodialysis (3.8%), and 4 of 144 1°-hyperparathyroidism patients (2.8%). Of four PCa patients who had a normal PTH ratio with the manual method, two had an inverted -generation PTH ratio with the automated method. Conclusions: Study of the -generation PTH ratio in large patient populations should be feasible using a mainstream automated platform like the Liaison XL. The current study confirms the utility of the inverted -generation PTH ratio as a marker of PCa (sensitivity: 81.8%; specificity: 97.3%). [less ▲]Detailed reference viewed: 77 (22 ULg)
Detection of decreased glomerular filtration rate in intensive care units: serum cystatin C versus serum creatinine
DELANAYE, Pierre ; CAVALIER, Etienne ; et al
in BMC Nephrology (2014), 15(9), 1471-2369Detailed reference viewed: 26 (9 ULg)
Enzymatic creatinine assays allowestimation of glomerular filtration rate in stages 1 and 2 chronic kidney disease using CKD-EPI equation
; ; CAVALIER, Etienne et al
in Clinica Chimica Acta (2014), 428
The National Kidney Disease Education Program group demonstrated that MDRD equation is sensitive to creatinine measurement error, particularly at higher glomerular filtration rates. Thus, MDRD-based eGFR ... [more ▼]
The National Kidney Disease Education Program group demonstrated that MDRD equation is sensitive to creatinine measurement error, particularly at higher glomerular filtration rates. Thus, MDRD-based eGFR above 60 mL/min/1.73 m2 should not be reported numerically. However, little is known about the impact of analytical error on CKD-EPI-based estimates. This study aimed at assessing the impact of analytical characteristics (bias and imprecision) of 12 enzymatic and 4 compensated Jaffe previously characterized creatinine assays on MDRD and CKD-EPI eGFR. In a simulation study, the impact of analytical error was assessed on a hospital population of 24 084 patients. Ability using each assay to correctly classify patients according to chronic kidney disease (CKD) stages was evaluated. For eGFR between 60 and 90 mL/min/1.73 m2, both equations were sensitive to analytical error. Compensated Jaffe assays displayed high bias in this range and led to poorer sensitivity/specificity for classification according to CKD stages than enzymatic assays. As compared to MDRD equation, CKD-EPI equation decreases impact of analytical error in creatinine measurement above 90 mL/min/1.73 m2. Compensated Jaffe creatinine assays lead to important errors in eGFR and should be avoided. Accurate enzymatic assays allow estimation of eGFR until 90 mL/min/1.73 m2 with MDRD and 120 mL/min/1.73 m2 with CKD-EPI equation. [less ▲]Detailed reference viewed: 32 (1 ULg)
Inter-method variability in bone alkaline phosphatase measurement: clinical impact on the management of dialysis patients.
DELANAYE, Pierre ; ; GADISSEUR, Romy et al
Poster (2014)Detailed reference viewed: 20 (6 ULg)
Mesure du NGAL dans le sang et les urines : comparaison des méthodes de mesure et éventuel impact sur la décision clinique.
DELANAYE, Pierre ; LAMBERMONT, Bernard ; MORIMONT, Philippe et al
Poster (2014)Detailed reference viewed: 17 (7 ULg)
Sclerostin concentration is not predictive of mortality in hemodialysis patients
DELANAYE, Pierre ; KRZESINSKI, Jean-Marie ; et al
Poster (2014)Detailed reference viewed: 23 (5 ULg)
Cockcroft&Gault and CKD-EPI equations: are these equations concordant to adjust drug dosage?
BOUQUEGNEAU, Antoine ; ; et al
Poster (2014)Detailed reference viewed: 17 (3 ULg)
Effet de la vitamine D native sur la concentration de parathormone chez le patient hémodialysé : y a-t-il un intérêt au dosage de la parathormone non oxydée ?
DELANAYE, Pierre ; DELANAYE, Pierre ; WEEKERS, Laurent et al
Poster (2014)Detailed reference viewed: 37 (9 ULg)
Impact of the stopping of vitamin K antagonist treatment on concentrations of Dephosphorylated and uncarboxylated Matrix Gla protein.
DELANAYE, Pierre ; DUBOIS, Bernard ; LUKAS, Pierre et al
Poster (2014)Detailed reference viewed: 19 (4 ULg)
Le néphrogramme isotopique (méthode de “Gates”) permet-il une estimation correcte du débit de filtration glomérulaire ? Revue de la littérature
DELANAYE, Pierre ; BECKERS, Catherine ; HUSTINX, Roland
Poster (2014)Detailed reference viewed: 13 (3 ULg)
Accuracy of Cockcroft&Gault and CKD-EPI equations to estimate glomerular filtration rate in obese population
BOUQUEGNEAU, Antoine ; ; et al
Poster (2014)Detailed reference viewed: 11 (2 ULg)
Estimation du DFG et adaptation posologique : la formule de Cockcroft-Gault et l’équation CKD-EPI désindexée sont-elles interchangeables ?
; ; et al
Poster (2014)Detailed reference viewed: 15 (0 ULg)
Estimateurs du débit de filtration glomérulaire : impact sur la prescription des antirétroviraux chez les sujets infectés par le VIH.
; DELANAYE, Pierre ; et al
Poster (2014)Detailed reference viewed: 4 (0 ULg)
Peut-on approcher l’ostéoporose de l’insuffisant rénal chronique par les biomarqueurs ?
Conference given outside the academic context (2014)Detailed reference viewed: 7 (0 ULg)
Nephrology and Clinical Chemistry: the essential link
Scientific conference (2014)Detailed reference viewed: 14 (0 ULg)