References of "Defraigne, Jean-Olivier"
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See detailActivation of the calcium-sensing receptor before renal ischemia/reperfusion exacerbates kidney injury
WEEKERS, Laurent ULg; De Tullio, Pascal ULg; BOVY, Christophe ULg et al

in American Journal of Translational Research (2015), 7(1), 128-138

Activation of the calcium-sensing receptor (CaSR) by ischemia/reperfusion (I/R) favours apoptosis in cardiomyocytes, hepatocytes and neurons. Its role in renal I/R is unknown. We investigated the impact ... [more ▼]

Activation of the calcium-sensing receptor (CaSR) by ischemia/reperfusion (I/R) favours apoptosis in cardiomyocytes, hepatocytes and neurons. Its role in renal I/R is unknown. We investigated the impact of pharmacological preactivation of the CaSR on kidney structure and function in a murine model of bilateral renal 30-min ischemia and 48-hour reperfusion, and in a 6-year cohort of kidney transplant recipients (KTR). C57BL/6J mice were administered daily with CaSR agonist, R-568, or with vehicle for 48 hours. Evaluation of serum urea and creatinine levels, renal histology and urine metabolome by nuclear magnetic resonance showed that R-568 was not nephrotoxic per se. Following I/R, serum urea and creatinine levels increased higher in R-568-treated animals than in controls. Jablonski’s score was significantly greater in R-568-treated kidneys, which showed a higher rate of cell proliferation and apoptosis in comparison to controls. Next, we retrospectively identified 36 patients (10.7% of our cohort) who were treated by CaSR agonist, cinacalcet, at the time of kidney transplantation (KTx). After matching these to 61 KTR upon type of donor, cold ischemic time, residual diuresis, and donor age, we observed that delayed graft function, i.e. need for dialysis in the first week after KTx, occurred in 42 and 23% of cinacalcet-treated and control groups, respectively (p≤0.05). These data suggest that pharmacological preactivation of the CaSR before renal I/R exacerbates kidney injury. [less ▲]

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See detailLow molecular weight poly (2-dimethylamino ethylmethacrylate) polymers with controlled positioned fluorescent labeling: Synthesis, characterization and in vitro interaction with human endothelial cells.
Flebus, Luca ULg; Lombart, François ULg; Sevrin, Chantal et al

in International journal of pharmaceutics (2015), 478(1), 278-287

Poly (2-dimethylamino ethylmethacrylate) (PDMAEMA) is an attractive non-degradable polymer studied as nonviral vector for gene delivery but it can be also adopted for delivery of other biopharmaceutical ... [more ▼]

Poly (2-dimethylamino ethylmethacrylate) (PDMAEMA) is an attractive non-degradable polymer studied as nonviral vector for gene delivery but it can be also adopted for delivery of other biopharmaceutical drugs. As a parenteral carrier, the PDMAEMA free form (FF) might interact with tissues and cells. Few data are available on its selective internalization and efflux from cells, while the majority of studies published have followed the distribution of DNA complexed with PDMAEMA. In order to address polycation safety, the first aim was to synthesize by atom transfer radical polymerisation (ATRP) fluorescent labeled PDMAEMA of low molecular weight (Mw) (below 15kDa), controlling the position and density of fluorescein. The second goal was to analyze the possible difference in uptake and subcellular distribution of this labeled FF polycation between human umbilical vein endothelial cells (HUVEC) and hCMEC/D3 cells. These two cell lines have been chosen in order to detect selectivity towards the blood-brain barrier (BBB). In both cases, polycation was detected along the plasma membrane followed by progressive migration to the peri-nuclear region, where it overlapped with lysosomal structures. The analysis by fluorescence-activated cell sorting (FACS) of the PDMAEMA uptake by hCMEC/D3 cells showed a significant (p<0.05) inhibition (40%) in presence of 2-dexoxy-d-glucose inhibitor, a result supporting an energy-dependence mechanism(s). Cytotoxicity study showed that low Mw PDMAEMA (10kDa) lead to a minor cytotoxicity compared to the higher ones. As main conclusion this study highlights the similitude in cell trafficking of FF PDMAEMA and data previously reported for PDMAEMA/DNA complexes. [less ▲]

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See detail(Tissue PET) Vascular metabolic imaging and peripheral plasma biomakers in the evolution of chronic arotic dissections
SAKALIHASAN, Natzi ULg; NIENABER, Christoph; HUSTINX, Roland ULg et al

in European Heart Journal - Cardiovascular Imaging (2015)

Enhanced FDG uptake may be considered as a complementary imaging marker associated with secondary complications in type B dissections. During follow-up, aneurysmal progression is related to PET/CT and ... [more ▼]

Enhanced FDG uptake may be considered as a complementary imaging marker associated with secondary complications in type B dissections. During follow-up, aneurysmal progression is related to PET/CT and biomarkers of thrombus renewal and lysis. [less ▲]

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See detailFamily Members of Patients with Abdominal Aortic Aneurysms are at Increased Risk for Aneurysms: Analysis of 618 Probands and their Families from the Liege AAA Family Study.
SakalihasanN, Natzi ULg; Defraigne, Jean-Olivier ULg; Kerstenne, Marie-Ange et al

in Annals of vascular surgery (2014), 28

BACKGROUND: The objectives were to answer the following questions using a well-characterized population in Liege, Belgium: 1) what percentage of abdominal aortic aneurysm (AAA) patients have a positive ... [more ▼]

BACKGROUND: The objectives were to answer the following questions using a well-characterized population in Liege, Belgium: 1) what percentage of abdominal aortic aneurysm (AAA) patients have a positive family history for AAA, 2) what is the prevalence of AAAs among relatives of AAA patients; and 3) do familial and sporadic AAA cases differ in clinical characteristics. METHODS AND RESULTS: Unrelated AAA patients diagnosed at the Cardiovascular Surgery Department, University Hospital of Liege, Belgium, between 1999 and 2012 were invited to the study. A detailed family history was obtained in interviews and recorded using Progeny software. In the initial interview 62 (10%) of the 618 AAA patients reported a positive family history for AAA. We divided the 618 patients into two study groups: Group I: 296 AAA patients (268; 91% males) were followed up with computerized tomography combined with positron emission tomography, and Group II: 322 AAA patients (295; 92% males) whose families were invited to ultrasonography screening. Ultrasonography screening identified 24 new AAAs among 186 relatives (>/= 50 years) of 144 families yielding a prevalence of 13%. The highest prevalence (25%) was found among brothers. By combining the number of AAAs found by ultrasonography screening with those diagnosed previously the observed lifetime prevalence of AAA was estimated to be 32% in brothers. The familial AAA cases were more likely to have a ruptured AAA than the sporadic cases (8% vs. 2.4%; P<0.0001). CONCLUSIONS: The findings confirm previously found high prevalence of AAA among brothers, support genetic contribution to AAA pathogenesis and provide rationale for targeted screening of relatives of AAA patients. [less ▲]

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See detailLa prothèse valvulaire idéale n’existe toujours pas. Quels facteurs entrent en compte pour orienter les choix d’une valve mécanique ou biologique ?
GREGOIRE, Céline ULg; Nellessen, Eric; Defraigne, Jean-Olivier ULg et al

in Revue Médicale de Liège (2014), 69(11), 600-604

The prevalence of valvular heart diseases reaches 2.5% in the overall population. Aortic valve replacement is one of the most common surgical procedures. We report the story of a female patient whose ... [more ▼]

The prevalence of valvular heart diseases reaches 2.5% in the overall population. Aortic valve replacement is one of the most common surgical procedures. We report the story of a female patient whose aortic mechanical valve, implanted at the age of 54 years at the time of a mitral valve repair surgery, had to be replaced 14 years later, due to the development of a subvalvular pannus narrowing the valvular orifice. We use this clinical story to compare the advantages and disadvantages of repair surgery and valve replacement with a biological or mechanical prosthesis, and summarize the latest evidence for the choice of the most adequate prosthesis for a particular patient’s profile. [less ▲]

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See detailPreliminary data from the Liège Screening programme suggests the reported decline in AAA prevalence is not global
Makrygiannis, Georgios ULg; EL HACHEMI, Mounia ULg; Labalue, Philippe et al

in Aorta (2014, October 01), 2(5), 212253

Background: Population based studies have shown evident benefit in terms of mortality from screening for Abdominal Aortic Aneurysm (AAA) among men aged over 65. However, recent studies from USA, UK and ... [more ▼]

Background: Population based studies have shown evident benefit in terms of mortality from screening for Abdominal Aortic Aneurysm (AAA) among men aged over 65. However, recent studies from USA, UK and Sweden suggest a decrease in the prevalence of AAA in the general population. Whether these findings are generalizable for the rest of Europe is unknown. Thus we decided to set up a screening program in order to detect AAA in Liege, Belgium. Material and Methods: During our ongoing study, over a first 3-month period, abdominal aortic ultrasound was performed on 541 participants, (198 women 343 men) to measure the maximal suprarenal and infrarenal aortic outer-outer diameter as well as the maximal diameter of the common iliac arteries. Moreover, we have measured arm and ankle blood pressure in each subject and the clinical characteristics of the patients, were collected. Results: The overall AAA prevalence was 4.62% (n = 25). In female participants aged ≥ 74 years the AAA prevalence amounted 1.01 %. While in male patients aged ≥ 65 years, it rose to 6.71%. Statistical analysis showed that male gender, aging, history of ischemic heart diseases, hyperlipidemia and varicose veins were significantly associated with AAA. Conclusion: Despite a reported declining prevalence of AAA in some recent population-based studies, we found that the prevalence of AAA in Liège population remains high in men aged 65 years or more. The prevalence of AAA seems to vary in different geographical regions. However, we need a larger sample to confirm our preliminary findings. [less ▲]

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See detailPREVENTION OF INCISIONAL HERNIA AFTER MIDLINE LAPAROTOMY FOR ABDOMINAL AORTIC ANEURYSM TREATMENT: A RANDOMIZED CONTROLLED TRIAL
Muysoms, F; Vierendeels, T; Huyghe, M et al

Conference (2014, September 11)

Background: The incidence of incisional hernias (IH) after open abdominal aortic aneurysm (AAA) repair is high. Prophylactic mesh augmentation (PMA) during laparotomy closure has been proposed in high ... [more ▼]

Background: The incidence of incisional hernias (IH) after open abdominal aortic aneurysm (AAA) repair is high. Prophylactic mesh augmentation (PMA) during laparotomy closure has been proposed in high-risk patients. Methods: A multicenter prospective randomized controlled study was conducted on patients undergoing elective repair of AAA through midline laparotomy (Clinical.Trials.gov: NCT00757133). In the study group a retro-muscular PMA was performed with a large-pore polypropylene mesh (Ultrapro™, width 7.5 cm). The primary endpoint was the incidence of IH at 24 months. Results: Between February 2009 and January 2013, 120 patients were recruited at 8 Belgian centers. Patients' characteristics at baseline were similar between the groups. Operative and postoperative characteristics showed no difference in morbidity or mortality. A highly significant reduction of IH incidence was found after PMA compared to conventional closure, respectively 0% (CI: 0 % - 5.5%) versus 27.6% (CI: 16.7% - 40.9%) (P<0.0001; Fisher's exact test). The estimated "freedom of IH" curves (Kaplan-Meier estimate) were significantly different across study arms (X2=18.93, P<0.0001; Mantel-Cox test). No adverse effects were observed, apart from an increased mean (SD) time to close the abdominal wall for PMA: 46.2 min (18.6) versus 29.6 min (18.5) (P<0.001; Mann-Whitney U test). Conclusion: Prophylactic retro-muscular mesh augmentation of a midline laparotomy in AAA patients is safe and effectively prevents the development of IH, with an extra time investment of 17 minutes. [less ▲]

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See detailDu processus aux soins intégrés : expérience de gestion de projet bottom-up
ERPICUM, Marie ULg; BASSLEER, Bernard ULg; Defraigne, Jean-Olivier ULg et al

Conference (2014, July 08)

Implementation of blood conservation strategies is a current issue in a public health view, especially in cardiac surgery. A service project has been initiated in the cardiovascular department of the CHU ... [more ▼]

Implementation of blood conservation strategies is a current issue in a public health view, especially in cardiac surgery. A service project has been initiated in the cardiovascular department of the CHU of Liege aiming to develop a blood conservation program. This project has evolved into the creation of a clinical pathway of the cardiac surgery patient and then into an institutional model for the development of other clinical pathways. This evolution leads to meet with the missions and strategic objectives of the Institution and some national projects. The process of this approach weaves a horizontal and vertical grid. The multidisciplinary membership, supported by medical and nursing leadership and the institutional support, will determine the sustainability of this project. [less ▲]

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See detailVascular Endothelial Growth Factor-111 (VEGF-111) and tendon healing: preliminary results in a rat model of tendon injury
Kaux, Jean-François ULg; Janssen, Lauriane ULg; Drion, Pierre ULg et al

in Muscles, Ligaments and Tendons Journal (2014), 4(1), 25-28

Tendon lesions are among the most frequent musculoskeletal pathologies. Vascular endothelial growth factor (VEGF) is known to regulate angiogenesis. VEGF-111, a biologically active and proteolysis ... [more ▼]

Tendon lesions are among the most frequent musculoskeletal pathologies. Vascular endothelial growth factor (VEGF) is known to regulate angiogenesis. VEGF-111, a biologically active and proteolysis-resistant splice variant of this family, was recently identified. This study aimed at evaluating whether VEGF-111 could have a therapeutic interest in tendon pathologies. Surgical section of one Achilles tendon of rats was performed before a local injection of either saline or VEGF-111. After 5, 15 and 30 days, the Achilles tendons of 10 rats of both groups were sampled and submitted to a biomechanical tensile test. The force necessary to induce tendon rupture was greater for tendons of the VEGF-111 group (p<0.05) while the section areas of the tendons were similar. The mechanical stress was similar at 5 and 15 days in the both groups but was improved for the VEGF-111 group at day 30 (p <0.001). No difference was observed in the mRNA expression of collagen III, tenomodulin and MMP-9. In conclusion, we observed that a local injection of VEGF-111 improves the early phases of the healing process of rat tendons after a surgical section. Further confirmatory experimentations are needed to consolidate our results. [less ▲]

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See detailDetermination of the plasma global antioxidant capacity: a critical review.
PINCEMAIL, Joël ULg; Cillard, Josiane; Neve, Jean et al

in Annales de biologie clinique (2014), 72(4), 413-421

With respect to prevention of cardiovascular diseases and cancers, the healthcare professionals are more and more interested in the blood determination of antioxidants (vitamins C and E, carotenoids ... [more ▼]

With respect to prevention of cardiovascular diseases and cancers, the healthcare professionals are more and more interested in the blood determination of antioxidants (vitamins C and E, carotenoids, glutathione, ubiquinone, antioxidant enzymes). The major problem of these analysis is their elevated cost. At the request of the healthcare professionals, the laboratories of clinical biology suggest the measurement of the plasma global antioxidant capacity (GAC) as a replacement of the individual determination of all these antioxidants. The present review shows that such a test presents a large number of gaps, the major one being that it essentially reflects the plasma concentration of uric acid and proteins. On basis of nine arguments, we show that the measurement of the plasma GAC cannot be considered as an in vivo marker of oxidative stress nor lead to the prescription antioxidant complement. [less ▲]

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See detailSex Differences in Abdominal Aortic Aneurysm: the Role of Sex Hormones.
Makrygiannis, Georgios ULg; Courtois, Audrey ULg; Drion, Pierre ULg et al

in Annals of vascular surgery (2014)

Abdominal aortic aneurysm (AAA) is a complex multifactorial disease with genetic and environmental components. AAA is more common in males, whereas women have a greater risk of rupture and more frequently ... [more ▼]

Abdominal aortic aneurysm (AAA) is a complex multifactorial disease with genetic and environmental components. AAA is more common in males, whereas women have a greater risk of rupture and more frequently have concomitant thoracic aortic aneurysms. Moreover, women are diagnosed with AAA about 10 years later and seem to be protected by female sex hormones. In this MEDLINE-based review of literature we examined human and animal, in vivo and in vitro studies, to further deepen our understanding of the sexual dimorphism of AAA. We focus on the role of sex hormones during the formation and growth of AAA. Endogenous estrogens and exogenous 17beta-estradiol were found to exert favorable actions protecting from AAA in animal models, whereas exogenous hormone replacement therapy in humans had inconclusive results. Androgens, known to have detrimental effects in the vasculature, in sufficient levels maintain the integrity of the aortic wall through their anabolic actions and act differentially in males and females, whereas lower levels of testosterone have been associated with AAA in humans. In conclusion, sex differences remain an important area of AAA research, but further studies especially in humans are needed. Furthermore, differential molecular mechanisms of sex hormones constitute a potential therapeutic target for AAA. [less ▲]

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See detailAntioxidant capacity of small dark fruits. Influence of cultivars and harvest time
Kevers, Claire ULg; PINCEMAIL, Joël ULg; Defraigne, Jean-Olivier ULg et al

in Journal of Berry Research (2014), 4

BACKGROUND: Small dark fruits represent one of the most important sources of bioactive compounds with antioxidant capacity in the human diet. The content of health-promoting antioxidants in these fruits ... [more ▼]

BACKGROUND: Small dark fruits represent one of the most important sources of bioactive compounds with antioxidant capacity in the human diet. The content of health-promoting antioxidants in these fruits may be important information to take into account when a fruit producer has to choose which cultivar to grow. OBJECTIVE: It is important to know how antioxidant capacity and antioxidant compounds as total phenolics and ascorbic acid vary between 9 small dark fruit species and for each species among cultivars (2 to 10 per species). METHODS: The antioxidant capacity (ORAC assay), total phenolic (Folin-Ciocalteu) and ascorbic acid content were measured in 9 fruits (plums, blackcurrants, blackberries, blueberries, cherries, redcurrants, raspberries, white currants and gooseberries) / 42 cultivars harvested at maturity during their high production period. RESULTS: The comparison of the average of the various cultivars of each small fruits showed that blackcurrants had the best antioxidant capacity (with plums), the highest ascorbic acid content and the highest total phenolic content (with blackberries). The present study shows that total phenolic compounds, ascorbic acid and antioxidant capacity strongly differed between genotypes of each small dark fruits. Other parameters as harvest time, culture conditions and maturity degree at the harvest may also influence the antioxidant capacity of small fruits. CONCLUSION: Among small dark fruits, blackcurrants have high qualities. Choices of variety, harvest time and maturity degree are important for all fruits. [less ▲]

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See detailAntioxidant capacity and phenolic composition of red wines from various grape varieties: Specificity of Pinot Noir.
Van Leeuw, Robin; Kevers, Claire ULg; PINCEMAIL, Joël ULg et al

in Journal of Food Composition and Analysis (2014), 36

Wines produced from various grape varieties present different properties affecting taste and color. The hypothesis was that grape genotype could have a deep impact on wine antioxidant properties and ... [more ▼]

Wines produced from various grape varieties present different properties affecting taste and color. The hypothesis was that grape genotype could have a deep impact on wine antioxidant properties and phenolic composition. But in this study on 38 different wines of 4 main grape varieties, large variability in the levels of individual phenolic compounds as well as in antioxidant capacity was observed in each grape variety. Comparisons of the wine varieties based on their individual phenolic profile (flavonols, anthocyanidins, flavan-3-ols, phenolic acids, resveratrol…) and antioxidant capacities (ORAC, DPPH, hemolysis, ESR, total phenolics) showed limited differences. An exception was the group of wines made from the grape variety Pinot Noir, in which the range of phenolic compounds was different from the other wines: anthocyanidins (87 mg L-1, 119 to 206 mg L-1 in other grape varieties) and flavonols (17 mg L-1, 20 to 57 mg L-1in others except Primitivo) showed lower levels while flavanols (327 mg L-1, 152 to 244 mg L-1 in others) and phenolic acids (161 mg L-1, 103 to 152 mg L-1 in others) showed levels higher than in the other wines. This different profile was associated with a lower antioxidant capacity (i.e. mean ORAC value: 20988 for Pinot Noir, 27820 to 33651 for others). [less ▲]

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See detailComment definir une circulation extracorporelle de qualite ?
Blaffart, F.; Defraigne, Jean-Olivier ULg

in Annales francaises d'anesthesie et de reanimation (2014)

Extracorporeal circulation (ECC) has to ensure homeostasis of the patient and to minimize pathophysiological disorders inherent in the technique. However, we cannot dissociate it from the surgery and ... [more ▼]

Extracorporeal circulation (ECC) has to ensure homeostasis of the patient and to minimize pathophysiological disorders inherent in the technique. However, we cannot dissociate it from the surgery and anesthesia knowing that the discipline involves a close partnership with these stakeholders. The purpose of this publication is to review the essential elements that at present can contribute to the quality of the CEC and suggest improvements and developments to be implemented in this area. [less ▲]

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