References of "Defaweux, Valérie"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailSpreading of prions from the immune to the peripheral nervous system: a potential implication of dendritic cells.
Dorban, Gauthier; Defaweux, Valérie ULg; Heinen, Ernst ULg et al

in Histochemistry & Cell Biology (2010), 133(5), 493-504

Detailed reference viewed: 34 (5 ULg)
See detailTravaux pratiques en Histologie Humaine, Quadrimestre 3
Defaweux, Valérie ULg; Mélot, France ULg; Florquin, Sandra ULg et al

Learning material (2010)

Detailed reference viewed: 60 (13 ULg)
See detailTravaux pratiques en Histologie Humaine, Quadrimestre 4, 5 et 6
Defaweux, Valérie ULg; Mélot, France ULg; Florquin, Sandra ULg et al

Learning material (2010)

Detailed reference viewed: 74 (15 ULg)
See detailImmunological discrimination between self and non self
Defaweux, Valérie ULg; Heinen, Ernst ULg

in Encyclopedia of Life Sciences (2010)

Detailed reference viewed: 43 (16 ULg)
Peer Reviewed
See detailVaccination with class-I restricted PrP peptides induces cytotoxic CD8+ T cells and prolongs the clinical phase duration of murine scrapie
Bruley Rosset, Martine; Sacquin, Antoine; Defaweux, Valérie ULg et al

Poster (2009, October)

Detailed reference viewed: 10 (2 ULg)
Full Text
Peer Reviewed
See detailGerminal centre innervation of bovine and human tonsils related to prion diseases.
Defaweux, Valérie ULg; Dorban, Gauthier; Antoine, Nadine ULg et al

in Brain, Behavior & Immunity (2009), 23(1), 10

Detailed reference viewed: 24 (7 ULg)
See detailIntroduction à la Cytologie et à l'Histologie générale
Defaweux, Valérie ULg

Learning material (2009)

Detailed reference viewed: 71 (8 ULg)
Peer Reviewed
See detailIn vitro modelisation of prions neuroinvasion mediated by dendritic cells
Dorban, G.; Defaweux, Valérie ULg; Heinen, Ernst ULg et al

Poster (2008, October)

Detailed reference viewed: 15 (10 ULg)
Full Text
Peer Reviewed
See detail. Do innervation of germinal centre and contacts between FDC and nerve fibers be keys to understand the susceptibility difference between bovines and humans to the BSE agent?
Defaweux, Valérie ULg; Dorban, G.; Antoine, Nadine ULg et al

Poster (2008, October)

Background: In regard to BSE and vCJD, the agent tropism for lymphoid tissues is completely different even if the infectious strain responsible and the way of inoculation are identical. During vCJD, the ... [more ▼]

Background: In regard to BSE and vCJD, the agent tropism for lymphoid tissues is completely different even if the infectious strain responsible and the way of inoculation are identical. During vCJD, the infectious agent crosses the digestive barrier and multiplies in lymphoid organs, before progressively reaching the brain. Indeed, in vCJD, it accumulates in the ileum, tonsils, spleen and appendix of infected individuals. In contrast, in cattle, the BSE agent has a low affinity for lymphoid tissues and mainly accumulates in the nervous system. During preclinical stages, infectivity, other than that in the peripheral nervous system or central nervous system, is confined in the distal ileum of orally infected cattle. So, it appears that, at least in the case of BSE and vCJD, host properties can influence the accumulation of the infectious agent in lymphoid organs. Objectives and methods: In this study, we analysed by confocale microscopy the mucosal innervation and the interface between nerve fibres and FDC in bovine and human tonsils using a panel of antibodies. Since differences in the innervation of lymphoid organs depending on species and on age have been reported, we analysed two categories of bovines (calves less than 12 months old and bovines older than 24 months) and two categories of humans (patients less than 5 years old and patients older than 25 years). Results: In both species, ways of innervation by-passing germinal centres could be postulated: nerve fibres are widely distributed in antigen/cell traffic area: the lamina propria, the interfollicular zone and the lymphoepithelial area. We pointed out that, only in tonsils of bovines older than 24 months, nerve fibres are observed to be in contact with FDC. In contrast, in human tonsils, no nerve fibres established contacts with FDC, whatever the age. Discussion: Innervation of germinal centres can be said to be an age-dependent dynamic process in bovines. The weak innervation of the secondary lymphoid organs could thus be a rate-limiting step to neuroinvasion in humans. This species difference could influence the way of neuroinvasion and thus, the susceptibility of bovines and humans to the BSE agent. [less ▲]

Detailed reference viewed: 13 (0 ULg)
Full Text
Peer Reviewed
See detailInteraction between dendritic cells and nerve fibres in lymphoid organs after oral scrapie exposure
Dorban, Gauthier ULg; Defaweux, Valérie ULg; Demonceau, Christine ULg et al

in Virchows Archiv (2007), 451(6), 1057-1065

In transmissible spongiform encephalopathies (TSEs), the infectious agent, called PrPsc, an abnormal isoform of the cellular prion protein, accumulates and replicates in lymphoid organs before affecting ... [more ▼]

In transmissible spongiform encephalopathies (TSEs), the infectious agent, called PrPsc, an abnormal isoform of the cellular prion protein, accumulates and replicates in lymphoid organs before affecting the nervous system. To clarify the cellular requirements for the neuro-invasion of the scrapie agent from the lymphoid organs to the central nervous system, we have studied, by confocal microscopy, the innervations within Peyer's patches, mesenteric lymph nodes and the spleen of mice in physiological conditions and after oral exposure to prion. Contacts between nerve fibres and PrPsc-associated cells, dendritic cells (DCs) and follicular dendritic cells (FDCs), were evaluated in preclinical prion-infected mice. Using a double immunolabelling strategy, we demonstrated the lack of innervation of PrPsc-accumulating cells (FDCs). Contacts between nerve fibers and PrPsc-propagating cells (DCs) were detected in T-cell zones and cell-trafficking areas. This supports, for the first time, the possible implication of dendritic cells in the prion neuroinvasion process. [less ▲]

Detailed reference viewed: 56 (16 ULg)
Peer Reviewed
See detailDialogue between peripheral nervous system and immunity: a confocal exploration
Dorban, G.; Defaweux, Valérie ULg; Demonceau, C. et al

Poster (2007, November)

Detailed reference viewed: 11 (5 ULg)
Full Text
Peer Reviewed
See detailOral scrapie infection modifies the homeostasis of Peyer's patches' dendritic cells
Dorban, Gauthier ULg; Defaweux, Valérie ULg; Levavasseur, Etienne et al

in Histochemistry & Cell Biology (2007), 128(3), 243-251

In transmitted prion diseases the immune system supports the replication and the propagation of the pathogenic agent (PrPSc). DCs, which are mobile cells present in large numbers within lymph organs, are ... [more ▼]

In transmitted prion diseases the immune system supports the replication and the propagation of the pathogenic agent (PrPSc). DCs, which are mobile cells present in large numbers within lymph organs, are suspected to carry prions through the lymphoid system and to transfer them towards the peripheral nervous system. In this study, C57Bl/6 mice were orally inoculated with PrPSc (scrapie strain 139A) and sacrificed at the preclinical stages of the disease. Immunolabelled cryosections of Peyer's patches were analysed by confocal microscopy. Membrane prion protein expression was studied by flow cytometry. In Peyer's patches (PP), dissected at day one and day 105 after oral exposure to scrapie, we observed an increased population of DCs localised in the follicular-associated epithelium. On day 105, PrPSc was found in the follicles inside the PP of prion-infected mice. A subset of Peyer's patches DCs, which did not express cellular prion protein on their surface in non-infected mice conditions, was prion-positive in scrapie conditions. Within Peyer's patches oral scrapie exposure thus induced modifications of the homeostasis of DCs at the preclinical stages of the disease. These results give new arguments in favour of the implication of DCs in prion diseases. [less ▲]

Detailed reference viewed: 35 (6 ULg)