References of "Deby-Dupont, G."
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See detailArachidonic acid and cyclooxygenase metabolism in acute lung injury
Lamy, Maurice ULg; Deby-Dupont, G.; Deby, C. et al

in Adult Respiratory Distress Syndrome (1992)

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See detailFast double antibody radioimmunoassay of human granulocyte myeloperoxidase and its application to plasma.
Pincemail, Joël ULg; Deby-Dupont, G.; Deby, Christiane ULg et al

in Journal of Immunological Methods (1991), 137(2), 181-191

The haem enzyme myeloperoxidase (MPO) (EC 1.11.1.7) with a spectral A430/A280 ratio greater than 0.7 and a specific activity of 125 U/mg was purified from isolated human neutrophils. To obtain a ... [more ▼]

The haem enzyme myeloperoxidase (MPO) (EC 1.11.1.7) with a spectral A430/A280 ratio greater than 0.7 and a specific activity of 125 U/mg was purified from isolated human neutrophils. To obtain a radioimmunoassay (RIA) for this enzyme, a specific antiserum against human neutrophil MPO was raised in rabbits and used at an initial dilution of 1/10,000. MPO labelled with 125iodine by a technique of self-labelling in the presence of H2O2, had a specific activity of 24 mCi/mg. After incubation at room temperature (2 h) and separation by double antibody precipitation in the presence of polyethylene glycol, the sensitivity of the RIA was 21 ng/ml. The RIA showed good precision and accuracy with intra- and interassay coefficients of variation of less than 7% for MPO concentrations ranging from 100 to 800 ng/ml, and satisfactory recoveries of known amounts of exogenous MPO in plasma. For the measurement of MPO in blood, the best sampling technique was to collect blood into EDTA. Rapid centrifugation (within 20 min) was necessary for blood collected into heparin. Mean MPO values in normal individuals were 340 +/- 98 ng/ml in EDTA plasma (n = 152) and 332 +/- 82 ng/ml in heparinized plasma (n = 34). When MPO was measured 12-6 h after injury in critically ill patients high values (above 1000 ng/ml) were found in 6/15 patients with multiple injuries. In patients with sepsis (n = 22), MPO values were always above 1000 ng/ml. [less ▲]

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See detailEquine Postanaesthetic Myositis: A Possible Role for Free Radical Generation and Membrane Lipoperoxidation
Serteyn, Didier ULg; Mottart, E.; Deby-Dupont, G. et al

in Research in Veterinary Science (1990), 48(1), 42-6

A method for the evaluation of total plasma antihydroxyl and antiperferryl activity is described. This method was applied to horse plasma obtained during halothane anaesthesia. In horses suffering from ... [more ▼]

A method for the evaluation of total plasma antihydroxyl and antiperferryl activity is described. This method was applied to horse plasma obtained during halothane anaesthesia. In horses suffering from postanaesthetic myositis, a significant decrease in the antiperferryl activity was observed during anaesthesia particularly when the muscular compression produced by the weight of the horse was released. In the affected muscles, strong oxidants could therefore be generated during the reperfusion of the ischaemic muscles and might initiate membrane lipid peroxidation. This phenomenon could possibly explain the muscular damage observed in equine postanaesthetic myositis. [less ▲]

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See detailProteases and antiproteases in adult respiratory distress syndrome
Deby-Dupont, G.; Lamy, Maurice ULg; Faymonville, Marie-Elisabeth ULg et al

in Acute Respiratory Failure Monograph Series: Lung Biology in Health and Disease (1989)

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See detailAcute-phase proteins and proteases-antiproteases in the inflammatory reaction
Lamy, Maurice ULg; Adam, A.; Deby-Dupont, G. et al

in New Horizons: Multiple Organ Failure (1989)

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See detailJustification biochimique du drainage du canal thoracique au cours de la pancréatite aiguë nécrotico-hémorragique
Deby-Dupont, G.; Reynaert, M.; Damas, Pierre ULg et al

in Acta Gastro-Enterologica Belgica (1988), 51(1), 31-40

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See detailA RIA for tumor necrosis factor (TNF a) and interleukin 1 a (IL-Ia) and their direct determination in serum
Reuter, A.; Bernier, J.; Gysen, P. et al

in Progress in Leukocyte Biology (1988)

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See detailShock pancreas
Deby-Dupont, G.; Faymonville, Marie-Elisabeth ULg; Damas, François ULg et al

in Intensive Care News (1988), 1

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See detailEtude du marquage de la beta-endorphine (beta-END) et de la beta-lipotropine (beta-LPH) par l'iode 125.
Deby-Dupont, G.; Reuter, A. M.; Joris, Jean ULg et al

in Comptes Rendus des Séances de la Société de Biologie et de ses Filiales (1983), 177(2), 259-68

5 micrograms of human beta-endorphin were labelled with 2 mCi 125I by the chloramine T technique. After two gel filtrations on Sephadex G-15 and on Sephadex G-50 in phosphate buffer with EDTA, Trasylol ... [more ▼]

5 micrograms of human beta-endorphin were labelled with 2 mCi 125I by the chloramine T technique. After two gel filtrations on Sephadex G-15 and on Sephadex G-50 in phosphate buffer with EDTA, Trasylol and mercapto-ethanol, a pure tracer was obtained with a specific activity about 150 microCi/ug. Kept at + 4 degrees C, the tracer remained utilizable for 30 days without loss of immunoreactivity. The labelling with lactoperoxydase and the use of another gel filtration method (filtration on Aca 202) gave a 125I beta-END tracer with the same immunoreactivity. The binding of this tracer to the antibody of an anti-beta-END antiserum diluted at 1/8000 was 32% with a non specific binding of 2%. 5 micrograms of human beta-lipotropin were labelled with 0.5 mCi 125I by the lactoperoxydase method. After two gel filtrations on Sephadex G-25 and on Sephadex G-75 in phosphate buffer with EDTA, Trasylol and mercapto-ethanol, a pure tracer with a specific activity of 140 microCi/micrograms was obtained. It remained utilizable for 30 days when kept at + 4 degrees C. Gel filtration on Aca 202 did not give good purification, while gel filtration on Aca 54 was good but slower than on Sephadex G-75. The binding to antibody in absence of unlabelled beta-LPH was 32% for an anti-beta-LPH antiserum diluted at 1/4000. The non specific binding was 2.5%. [less ▲]

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