Equine Postanaesthetic Myositis: A Possible Role for Free Radical Generation and Membrane Lipoperoxidation

in Research in Veterinary Science (1990), 48(1), 42-6

A method for the evaluation of total plasma antihydroxyl and antiperferryl activity is described. This method was applied to horse plasma obtained during halothane anaesthesia. In horses suffering from ... [more ▼]

A method for the evaluation of total plasma antihydroxyl and antiperferryl activity is described. This method was applied to horse plasma obtained during halothane anaesthesia. In horses suffering from postanaesthetic myositis, a significant decrease in the antiperferryl activity was observed during anaesthesia particularly when the muscular compression produced by the weight of the horse was released. In the affected muscles, strong oxidants could therefore be generated during the reperfusion of the ischaemic muscles and might initiate membrane lipid peroxidation. This phenomenon could possibly explain the muscular damage observed in equine postanaesthetic myositis. [less ▲]

Acute-phase proteins and proteases-antiproteases in the inflammatory reaction

in New Horizons: Multiple Organ Failure (1989)

A RIA for tumor necrosis factor (TNF a) and interleukin 1 a (IL-Ia) and their direct determination in serum

in Progress in Leukocyte Biology (1988)

Etude du marquage de la beta-endorphine (beta-END) et de la beta-lipotropine (beta-LPH) par l'iode 125.

in Comptes Rendus des Séances de la Société de Biologie et de ses Filiales (1983), 177(2), 259-68

5 micrograms of human beta-endorphin were labelled with 2 mCi 125I by the chloramine T technique. After two gel filtrations on Sephadex G-15 and on Sephadex G-50 in phosphate buffer with EDTA, Trasylol ... [more ▼]

5 micrograms of human beta-endorphin were labelled with 2 mCi 125I by the chloramine T technique. After two gel filtrations on Sephadex G-15 and on Sephadex G-50 in phosphate buffer with EDTA, Trasylol and mercapto-ethanol, a pure tracer was obtained with a specific activity about 150 microCi/ug. Kept at + 4 degrees C, the tracer remained utilizable for 30 days without loss of immunoreactivity. The labelling with lactoperoxydase and the use of another gel filtration method (filtration on Aca 202) gave a 125I beta-END tracer with the same immunoreactivity. The binding of this tracer to the antibody of an anti-beta-END antiserum diluted at 1/8000 was 32% with a non specific binding of 2%. 5 micrograms of human beta-lipotropin were labelled with 0.5 mCi 125I by the lactoperoxydase method. After two gel filtrations on Sephadex G-25 and on Sephadex G-75 in phosphate buffer with EDTA, Trasylol and mercapto-ethanol, a pure tracer with a specific activity of 140 microCi/micrograms was obtained. It remained utilizable for 30 days when kept at + 4 degrees C. Gel filtration on Aca 202 did not give good purification, while gel filtration on Aca 54 was good but slower than on Sephadex G-75. The binding to antibody in absence of unlabelled beta-LPH was 32% for an anti-beta-LPH antiserum diluted at 1/4000. The non specific binding was 2.5%. [less ▲]